Reviewed, UniProtKB/Swiss-Prot Q03346 (MPPB_RAT)

Names and origin

Protein names

Recommended name:
    Mitochondrial-processing peptidase subunit beta
    EC=3.4.24.64
Alternative name(s):
    Beta-MPP
    P-52

Gene names

Name:	Pmpcb
Synonyms:	Mppb

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cleaves presequences (transit peptides) from mitochondrial protein precursors.
Catalytic activity	Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Heterodimer of alpha and beta subunits.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the peptidase M16 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	mitochondrial protein processing during import Ref.1 Traceable author statement. Source: RGD proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 45	45	Mitochondrion Potential
	Chain	46 – 489	444	Mitochondrial-processing peptidase subunit beta		PRO_0000026779
Sites
	Active site	104	1	Proton acceptor By similarity
	Metal binding	101	1	Zinc By similarity
	Metal binding	105	1	Zinc By similarity
	Metal binding	181	1	Zinc By similarity
Experimental info
	Sequence conflict	167	1	G → R in BAA03007. Ref.3
	Sequence conflict	312	1	C → R in AAA41633. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q03346-1 [UniParc]. Last modified January 9, 2007. Version 3. Checksum: 79B9C1ED7385533F	FASTA	489	54,265
	10 20 30 40 50 60 MAAAAVSRTL LPVAGRRLWG FTRRLPLRAA AAQPLYFGGD RLRSTQAAPQ VVLNVPETQV 70 80 90 100 110 120 TCLENGLRVA SENSGISTCT VGLWIDAGSR YENEKNNGTA HFLEHMAFKG TKKRSQLDLE 130 140 150 160 170 180 LEIENMGAHL NAYTSREQTV YYAKAFSKDL PRAVEILADI IQNSTLGEAE IERERGVILR 190 200 210 220 230 240 EMQEVETNLQ EVVFDYLHAT AYQNTALGRT ILGPTENIKS ISRKDLVDYI TTHYKGPRIV 250 260 270 280 290 300 LAAAGGVCHN ELLELAKFHF GDSLCAHKGD VPALPPCKFT GSEIRVRDDK MPLAHLAVAI 310 320 330 340 350 360 EAVGWTHPDT ICLMVANTLI GNWDRSFGGG MNLSSKLAQL TCHGNLCHSF QSFNTSYTDT 370 380 390 400 410 420 GLWGLYMVCE QATVADMLHA VQKEWMRLCT AVSESEVARA KNLLKTNMLL QLDGSTPICE 430 440 450 460 470 480 DIGRQMLCYN RRIPIPELEA RIDAVDAEMV REVCTKYIYG KSPAIAALGP IERLPDFNQI CSNMRWTRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The beta subunit of the mitochondrial processing peptidase from rat liver: cloning and sequencing of a cDNA and comparison with a proposed family of metallopeptidases." Paces V., Rosenberg L.E., Fenton W.A., Kalousek F. Proc. Natl. Acad. Sci. U.S.A. 90:5355-5358(1993) [PubMed: 8506385] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	"Molecular cloning of the smaller subunit(P52) of rat liver mitochondrial processing protease." Kitada S., Niidome T., Nagano T., Ogishima T., Ito A. Biochem. Biophys. Res. Commun. 190:289-293(1993) [PubMed: 8422255] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-489. Tissue: Liver.
[4]	Kitada S. Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[5]	Lubec G., Afjehi-Sadat L. Submitted (NOV-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 153-173, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Spinal cord.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L12965 mRNA. Translation: AAA41633.1. BC078826 mRNA. Translation: AAH78826.1. D13907 mRNA. Translation: BAA03007.1.
IPI	IPI00209980.
PIR	S36390.
RefSeq	NP_071790.1.
UniGene	Rn.841
3D structure databases
HSSP	HSSP built from PDB template 1HR6 based on UniProtKB P10507.
ModBase	Search...
Protein family/group databases
MEROPS	M16.003. M16.980.
Genome annotation databases
Ensembl	ENSRNOG00000012693. Rattus norvegicus. [Contig view]
GeneID	64198.
KEGG	rno:64198.
NMPDR	fig|10116.3.peg.20650.
Organism-specific databases
RGD	621297. Pmpcb.
Phylogenomic databases
HOVERGEN	Q03346.
OMA	Q03346. IPLMVAN.
Enzyme and pathway databases
BRENDA	3.4.24.64. 248.
Gene expression databases
ArrayExpress	Q03346.
GermOnline	ENSRNOG00000012693. Rattus norvegicus.
Family and domain databases
InterPro	IPR011237. Pept_M16_core. IPR011765. Pept_M16_N. IPR001431. Pept_M16_Zn_BS. IPR007863. Peptidase_M16_C. [Graphical view]
Gene3D	G3DSA:3.30.830.10. Pept_M16_core. 2 hits.
Pfam	PF00675. Peptidase_M16. 1 hit. PF05193. Peptidase_M16_C. 1 hit. [Graphical view]
PROSITE	PS00143. INSULINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	612876.

Entry information

Entry name

MPPB_RAT

Accession

Primary (citable) accession number: Q03346
Secondary accession number(s): Q68G08

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 9, 2007
Last modified:	June 16, 2009
This is version 75 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)