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Q03338 (PRP3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
U4/U6 small nuclear ribonucleoprotein PRP3
Alternative name(s):
Pre-mRNA-splicing factor 3
Gene names
Name:PRP3
Synonyms:RNA3
Ordered Locus Names:YDR473C
ORF Names:D8035.16
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. Ref.3

Subunit structure

Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1. Ref.4 Ref.7

Subcellular location

Nucleus.

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PRP4P200535EBI-421,EBI-219

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 469469U4/U6 small nuclear ribonucleoprotein PRP3
PRO_0000097037

Sequences

Sequence LengthMass (Da)Tools
Q03338 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: E977FD9F708BDD91

FASTA46955,870
        10         20         30         40         50         60 
MPPRNTYEKG NPKRQNSPYY KPSFLRREET TNDEEKFQGH GLKTELHSAL KSSNLNLIRR 

        70         80         90        100        110        120 
TYQTGENPYL SDPHDRGSSS RFNRRYERGL KFYQKGEISK RIAQERTLQK QQEEEELKRK 

       130        140        150        160        170        180 
LKQEEDEKDK RKLIESGDLP NLELHEDKFL LDLSKFKIYY DNNHGYEWWD TAYLDEKGEL 

       190        200        210        220        230        240 
MEKYDMNGTS PAEEKLAEDI DEVDDDDDDE HPSIRYVAHP LPEKINEAKV SIKAYLTQHE 

       250        260        270        280        290        300 
RKRLRRNRRK MAREAREIKI KLGLLPKPEP KVKLSNMMSV FENDQNITDP TAWEKVVKDQ 

       310        320        330        340        350        360 
VDLRKRKHLE ENERRHEDAI KRRKEAVNMN VEKPTVYHCK VFQFKNLQNP KIRFKLKMNS 

       370        380        390        400        410        420 
KELSLKGLCL RIRDDGPGII IVVGNEKSCK FYENLVMKRI KWNEDFELHT NTGDIKMDMH 

       430        440        450        460 
NNSISKTWEG YLQDCKFKGW FMKVCNDQDS LLRTLGQFDS EHFYSPVQT 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"The yeast Prp3 protein is a U4/U6 snRNP protein necessary for integrity of the U4/U6 snRNP and the U4/U6.U5 tri-snRNP."
Anthony J.G., Weidenhammer E.M., Woolford J.L. Jr.
RNA 3:1143-1152(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."
Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P.
EMBO J. 18:4535-4548(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy."
Hacker I., Sander B., Golas M.M., Wolf E., Karagoz E., Kastner B., Stark H., Fabrizio P., Luhrmann R.
Nat. Struct. Mol. Biol. 15:1206-1212(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, ELECTRON MICROSCOPY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U33050 Genomic DNA. Translation: AAB64932.1.
BK006938 Genomic DNA. Translation: DAA12307.1.
PIRS69640.
RefSeqNP_010761.3. NM_001180781.3.

3D structure databases

ProteinModelPortalQ03338.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32526. 53 interactions.
DIPDIP-5266N.
IntActQ03338. 27 interactions.
MINTMINT-490402.
STRING4932.YDR473C.

Proteomic databases

PaxDbQ03338.
PRIDEQ03338.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR473C; YDR473C; YDR473C.
GeneID852084.
KEGGsce:YDR473C.

Organism-specific databases

CYGDYDR473c.
SGDS000002881. PRP3.

Phylogenomic databases

eggNOGNOG268901.
GeneTreeENSGT00390000011497.
HOGENOMHOG000205412.
KOK12843.
OrthoDBEOG74XSH4.

Enzyme and pathway databases

BioCycYEAST:G3O-30000-MONOMER.

Gene expression databases

GenevestigatorQ03338.

Family and domain databases

InterProIPR010541. DUF1115.
IPR013881. Pre-mRNA_splic_Prp3.
IPR027104. Prp3.
[Graphical view]
PANTHERPTHR14212. PTHR14212. 1 hit.
PfamPF06544. DUF1115. 1 hit.
PF08572. PRP3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio970400.
PROQ03338.

Entry information

Entry namePRP3_YEAST
AccessionPrimary (citable) accession number: Q03338
Secondary accession number(s): D6VT97
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD