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Protein

U4/U6 small nuclear ribonucleoprotein PRP3

Gene

PRP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.1 Publication

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-30000-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6 small nuclear ribonucleoprotein PRP3
Alternative name(s):
Pre-mRNA-splicing factor 3
Gene namesi
Name:PRP3
Synonyms:RNA3
Ordered Locus Names:YDR473C
ORF Names:D8035.16
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR473C
SGDiS000002881 PRP3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000970371 – 469U4/U6 small nuclear ribonucleoprotein PRP3Add BLAST469

Proteomic databases

MaxQBiQ03338
PaxDbiQ03338
PRIDEiQ03338

PTM databases

iPTMnetiQ03338

Interactioni

Subunit structurei

Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32526175 interactors.
DIPiDIP-5266N
IntActiQ03338 44 interactors.
MINTiQ03338
STRINGi4932.YDR473C

Structurei

Secondary structure

1469
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi337 – 345Combined sources9
Helixi350 – 363Combined sources14
Beta strandi366 – 373Combined sources8
Beta strandi378 – 384Combined sources7
Helixi386 – 398Combined sources13
Beta strandi406 – 409Combined sources4
Beta strandi414 – 417Combined sources4
Beta strandi424 – 433Combined sources10
Beta strandi439 – 447Combined sources9
Helixi448 – 457Combined sources10
Helixi461 – 463Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCMelectron microscopy3.80K1-469[»]
4YHUX-ray2.70A/B/C296-469[»]
4YHVX-ray2.00A325-469[»]
4YHWX-ray3.25A/B296-469[»]
5GANelectron microscopy3.60G1-469[»]
5GAPelectron microscopy3.60G1-469[»]
5NRLelectron microscopy7.20G1-469[»]
ProteinModelPortaliQ03338
SMRiQ03338
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

GeneTreeiENSGT00390000011497
HOGENOMiHOG000205412
InParanoidiQ03338
KOiK12843
OrthoDBiEOG092C237O

Family and domain databases

InterProiView protein in InterPro
IPR010541 DUF1115
IPR013881 Pre-mRNA_splic_Prp3
IPR027104 Prp3
PANTHERiPTHR14212 PTHR14212, 1 hit
PfamiView protein in Pfam
PF06544 DUF1115, 1 hit
PF08572 PRP3, 1 hit

Sequencei

Sequence statusi: Complete.

Q03338-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPRNTYEKG NPKRQNSPYY KPSFLRREET TNDEEKFQGH GLKTELHSAL
60 70 80 90 100
KSSNLNLIRR TYQTGENPYL SDPHDRGSSS RFNRRYERGL KFYQKGEISK
110 120 130 140 150
RIAQERTLQK QQEEEELKRK LKQEEDEKDK RKLIESGDLP NLELHEDKFL
160 170 180 190 200
LDLSKFKIYY DNNHGYEWWD TAYLDEKGEL MEKYDMNGTS PAEEKLAEDI
210 220 230 240 250
DEVDDDDDDE HPSIRYVAHP LPEKINEAKV SIKAYLTQHE RKRLRRNRRK
260 270 280 290 300
MAREAREIKI KLGLLPKPEP KVKLSNMMSV FENDQNITDP TAWEKVVKDQ
310 320 330 340 350
VDLRKRKHLE ENERRHEDAI KRRKEAVNMN VEKPTVYHCK VFQFKNLQNP
360 370 380 390 400
KIRFKLKMNS KELSLKGLCL RIRDDGPGII IVVGNEKSCK FYENLVMKRI
410 420 430 440 450
KWNEDFELHT NTGDIKMDMH NNSISKTWEG YLQDCKFKGW FMKVCNDQDS
460
LLRTLGQFDS EHFYSPVQT
Length:469
Mass (Da):55,870
Last modified:November 1, 1996 - v1
Checksum:iE977FD9F708BDD91
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA Translation: AAB64932.1
BK006938 Genomic DNA Translation: DAA12307.1
PIRiS69640
RefSeqiNP_010761.3, NM_001180781.3

Genome annotation databases

EnsemblFungiiYDR473C; YDR473C; YDR473C
GeneIDi852084
KEGGisce:YDR473C

Similar proteinsi

Entry informationi

Entry nameiPRP3_YEAST
AccessioniPrimary (citable) accession number: Q03338
Secondary accession number(s): D6VT97
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome