Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q03338

- PRP3_YEAST

UniProt

Q03338 - PRP3_YEAST

Protein

U4/U6 small nuclear ribonucleoprotein PRP3

Gene

PRP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: SGD

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30000-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    U4/U6 small nuclear ribonucleoprotein PRP3
    Alternative name(s):
    Pre-mRNA-splicing factor 3
    Gene namesi
    Name:PRP3
    Synonyms:RNA3
    Ordered Locus Names:YDR473C
    ORF Names:D8035.16
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR473c.
    SGDiS000002881. PRP3.

    Subcellular locationi

    GO - Cellular componenti

    1. spliceosomal complex Source: UniProtKB-KW
    2. U4/U6 x U5 tri-snRNP complex Source: SGD

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 469469U4/U6 small nuclear ribonucleoprotein PRP3PRO_0000097037Add
    BLAST

    Proteomic databases

    MaxQBiQ03338.
    PaxDbiQ03338.
    PRIDEiQ03338.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03338.

    Interactioni

    Subunit structurei

    Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PRP4P200535EBI-421,EBI-219

    Protein-protein interaction databases

    BioGridi32526. 53 interactions.
    DIPiDIP-5266N.
    IntActiQ03338. 27 interactions.
    MINTiMINT-490402.
    STRINGi4932.YDR473C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03338.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG268901.
    GeneTreeiENSGT00390000011497.
    HOGENOMiHOG000205412.
    KOiK12843.
    OMAiNERRHED.
    OrthoDBiEOG74XSH4.

    Family and domain databases

    InterProiIPR010541. DUF1115.
    IPR013881. Pre-mRNA_splic_Prp3.
    IPR027104. Prp3.
    [Graphical view]
    PANTHERiPTHR14212. PTHR14212. 1 hit.
    PfamiPF06544. DUF1115. 1 hit.
    PF08572. PRP3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03338-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPRNTYEKG NPKRQNSPYY KPSFLRREET TNDEEKFQGH GLKTELHSAL    50
    KSSNLNLIRR TYQTGENPYL SDPHDRGSSS RFNRRYERGL KFYQKGEISK 100
    RIAQERTLQK QQEEEELKRK LKQEEDEKDK RKLIESGDLP NLELHEDKFL 150
    LDLSKFKIYY DNNHGYEWWD TAYLDEKGEL MEKYDMNGTS PAEEKLAEDI 200
    DEVDDDDDDE HPSIRYVAHP LPEKINEAKV SIKAYLTQHE RKRLRRNRRK 250
    MAREAREIKI KLGLLPKPEP KVKLSNMMSV FENDQNITDP TAWEKVVKDQ 300
    VDLRKRKHLE ENERRHEDAI KRRKEAVNMN VEKPTVYHCK VFQFKNLQNP 350
    KIRFKLKMNS KELSLKGLCL RIRDDGPGII IVVGNEKSCK FYENLVMKRI 400
    KWNEDFELHT NTGDIKMDMH NNSISKTWEG YLQDCKFKGW FMKVCNDQDS 450
    LLRTLGQFDS EHFYSPVQT 469
    Length:469
    Mass (Da):55,870
    Last modified:November 1, 1996 - v1
    Checksum:iE977FD9F708BDD91
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33050 Genomic DNA. Translation: AAB64932.1.
    BK006938 Genomic DNA. Translation: DAA12307.1.
    PIRiS69640.
    RefSeqiNP_010761.3. NM_001180781.3.

    Genome annotation databases

    EnsemblFungiiYDR473C; YDR473C; YDR473C.
    GeneIDi852084.
    KEGGisce:YDR473C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33050 Genomic DNA. Translation: AAB64932.1 .
    BK006938 Genomic DNA. Translation: DAA12307.1 .
    PIRi S69640.
    RefSeqi NP_010761.3. NM_001180781.3.

    3D structure databases

    ProteinModelPortali Q03338.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32526. 53 interactions.
    DIPi DIP-5266N.
    IntActi Q03338. 27 interactions.
    MINTi MINT-490402.
    STRINGi 4932.YDR473C.

    Proteomic databases

    MaxQBi Q03338.
    PaxDbi Q03338.
    PRIDEi Q03338.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR473C ; YDR473C ; YDR473C .
    GeneIDi 852084.
    KEGGi sce:YDR473C.

    Organism-specific databases

    CYGDi YDR473c.
    SGDi S000002881. PRP3.

    Phylogenomic databases

    eggNOGi NOG268901.
    GeneTreei ENSGT00390000011497.
    HOGENOMi HOG000205412.
    KOi K12843.
    OMAi NERRHED.
    OrthoDBi EOG74XSH4.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30000-MONOMER.

    Miscellaneous databases

    NextBioi 970400.
    PROi Q03338.

    Gene expression databases

    Genevestigatori Q03338.

    Family and domain databases

    InterProi IPR010541. DUF1115.
    IPR013881. Pre-mRNA_splic_Prp3.
    IPR027104. Prp3.
    [Graphical view ]
    PANTHERi PTHR14212. PTHR14212. 1 hit.
    Pfami PF06544. DUF1115. 1 hit.
    PF08572. PRP3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "The yeast Prp3 protein is a U4/U6 snRNP protein necessary for integrity of the U4/U6 snRNP and the U4/U6.U5 tri-snRNP."
      Anthony J.G., Weidenhammer E.M., Woolford J.L. Jr.
      RNA 3:1143-1152(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."
      Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P.
      EMBO J. 18:4535-4548(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy."
      Hacker I., Sander B., Golas M.M., Wolf E., Karagoz E., Kastner B., Stark H., Fabrizio P., Luhrmann R.
      Nat. Struct. Mol. Biol. 15:1206-1212(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, ELECTRON MICROSCOPY.

    Entry informationi

    Entry nameiPRP3_YEAST
    AccessioniPrimary (citable) accession number: Q03338
    Secondary accession number(s): D6VT97
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2003
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 432 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3