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Protein

U4/U6 small nuclear ribonucleoprotein PRP3

Gene

PRP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.1 Publication

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-30000-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6 small nuclear ribonucleoprotein PRP3
Alternative name(s):
Pre-mRNA-splicing factor 3
Gene namesi
Name:PRP3
Synonyms:RNA3
Ordered Locus Names:YDR473C
ORF Names:D8035.16
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR473C.
SGDiS000002881. PRP3.

Subcellular locationi

GO - Cellular componenti

  • spliceosomal complex Source: UniProtKB-KW
  • U4/U6 x U5 tri-snRNP complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000970371 – 469U4/U6 small nuclear ribonucleoprotein PRP3Add BLAST469

Proteomic databases

MaxQBiQ03338.
PRIDEiQ03338.

PTM databases

iPTMnetiQ03338.

Interactioni

Subunit structurei

Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRP4P200535EBI-421,EBI-219

Protein-protein interaction databases

BioGridi32526. 45 interactors.
DIPiDIP-5266N.
IntActiQ03338. 28 interactors.
MINTiMINT-490402.

Structurei

Secondary structure

1469
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi337 – 345Combined sources9
Helixi350 – 363Combined sources14
Beta strandi366 – 373Combined sources8
Beta strandi378 – 384Combined sources7
Helixi386 – 398Combined sources13
Beta strandi406 – 409Combined sources4
Beta strandi414 – 417Combined sources4
Beta strandi424 – 433Combined sources10
Beta strandi439 – 447Combined sources9
Helixi448 – 457Combined sources10
Helixi461 – 463Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCMelectron microscopy3.80K1-469[»]
4YHUX-ray2.70A/B/C296-469[»]
4YHVX-ray2.00A325-469[»]
4YHWX-ray3.25A/B296-469[»]
5GANelectron microscopy3.60G1-469[»]
5GAPelectron microscopy3.60G1-469[»]
ProteinModelPortaliQ03338.
SMRiQ03338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

GeneTreeiENSGT00390000011497.
HOGENOMiHOG000205412.
InParanoidiQ03338.
KOiK12843.
OrthoDBiEOG092C237O.

Family and domain databases

InterProiIPR010541. DUF1115.
IPR013881. Pre-mRNA_splic_Prp3.
IPR027104. Prp3.
[Graphical view]
PANTHERiPTHR14212. PTHR14212. 1 hit.
PfamiPF06544. DUF1115. 1 hit.
PF08572. PRP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03338-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPRNTYEKG NPKRQNSPYY KPSFLRREET TNDEEKFQGH GLKTELHSAL
60 70 80 90 100
KSSNLNLIRR TYQTGENPYL SDPHDRGSSS RFNRRYERGL KFYQKGEISK
110 120 130 140 150
RIAQERTLQK QQEEEELKRK LKQEEDEKDK RKLIESGDLP NLELHEDKFL
160 170 180 190 200
LDLSKFKIYY DNNHGYEWWD TAYLDEKGEL MEKYDMNGTS PAEEKLAEDI
210 220 230 240 250
DEVDDDDDDE HPSIRYVAHP LPEKINEAKV SIKAYLTQHE RKRLRRNRRK
260 270 280 290 300
MAREAREIKI KLGLLPKPEP KVKLSNMMSV FENDQNITDP TAWEKVVKDQ
310 320 330 340 350
VDLRKRKHLE ENERRHEDAI KRRKEAVNMN VEKPTVYHCK VFQFKNLQNP
360 370 380 390 400
KIRFKLKMNS KELSLKGLCL RIRDDGPGII IVVGNEKSCK FYENLVMKRI
410 420 430 440 450
KWNEDFELHT NTGDIKMDMH NNSISKTWEG YLQDCKFKGW FMKVCNDQDS
460
LLRTLGQFDS EHFYSPVQT
Length:469
Mass (Da):55,870
Last modified:November 1, 1996 - v1
Checksum:iE977FD9F708BDD91
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA. Translation: AAB64932.1.
BK006938 Genomic DNA. Translation: DAA12307.1.
PIRiS69640.
RefSeqiNP_010761.3. NM_001180781.3.

Genome annotation databases

EnsemblFungiiYDR473C; YDR473C; YDR473C.
GeneIDi852084.
KEGGisce:YDR473C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA. Translation: AAB64932.1.
BK006938 Genomic DNA. Translation: DAA12307.1.
PIRiS69640.
RefSeqiNP_010761.3. NM_001180781.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCMelectron microscopy3.80K1-469[»]
4YHUX-ray2.70A/B/C296-469[»]
4YHVX-ray2.00A325-469[»]
4YHWX-ray3.25A/B296-469[»]
5GANelectron microscopy3.60G1-469[»]
5GAPelectron microscopy3.60G1-469[»]
ProteinModelPortaliQ03338.
SMRiQ03338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32526. 45 interactors.
DIPiDIP-5266N.
IntActiQ03338. 28 interactors.
MINTiMINT-490402.

PTM databases

iPTMnetiQ03338.

Proteomic databases

MaxQBiQ03338.
PRIDEiQ03338.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR473C; YDR473C; YDR473C.
GeneIDi852084.
KEGGisce:YDR473C.

Organism-specific databases

EuPathDBiFungiDB:YDR473C.
SGDiS000002881. PRP3.

Phylogenomic databases

GeneTreeiENSGT00390000011497.
HOGENOMiHOG000205412.
InParanoidiQ03338.
KOiK12843.
OrthoDBiEOG092C237O.

Enzyme and pathway databases

BioCyciYEAST:G3O-30000-MONOMER.

Miscellaneous databases

PROiQ03338.

Family and domain databases

InterProiIPR010541. DUF1115.
IPR013881. Pre-mRNA_splic_Prp3.
IPR027104. Prp3.
[Graphical view]
PANTHERiPTHR14212. PTHR14212. 1 hit.
PfamiPF06544. DUF1115. 1 hit.
PF08572. PRP3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRP3_YEAST
AccessioniPrimary (citable) accession number: Q03338
Secondary accession number(s): D6VT97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.