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Q03338

- PRP3_YEAST

UniProt

Q03338 - PRP3_YEAST

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Protein

U4/U6 small nuclear ribonucleoprotein PRP3

Gene

PRP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.1 Publication

GO - Biological processi

  1. mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-30000-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6 small nuclear ribonucleoprotein PRP3
Alternative name(s):
Pre-mRNA-splicing factor 3
Gene namesi
Name:PRP3
Synonyms:RNA3
Ordered Locus Names:YDR473C
ORF Names:D8035.16
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR473c.
SGDiS000002881. PRP3.

Subcellular locationi

GO - Cellular componenti

  1. spliceosomal complex Source: UniProtKB-KW
  2. U4/U6 x U5 tri-snRNP complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469U4/U6 small nuclear ribonucleoprotein PRP3PRO_0000097037Add
BLAST

Proteomic databases

MaxQBiQ03338.
PaxDbiQ03338.
PRIDEiQ03338.

Expressioni

Gene expression databases

GenevestigatoriQ03338.

Interactioni

Subunit structurei

Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRP4P200535EBI-421,EBI-219

Protein-protein interaction databases

BioGridi32526. 53 interactions.
DIPiDIP-5266N.
IntActiQ03338. 27 interactions.
MINTiMINT-490402.
STRINGi4932.YDR473C.

Structurei

3D structure databases

ProteinModelPortaliQ03338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG268901.
GeneTreeiENSGT00390000011497.
HOGENOMiHOG000205412.
InParanoidiQ03338.
KOiK12843.
OMAiNERRHED.
OrthoDBiEOG74XSH4.

Family and domain databases

InterProiIPR010541. DUF1115.
IPR013881. Pre-mRNA_splic_Prp3.
IPR027104. Prp3.
[Graphical view]
PANTHERiPTHR14212. PTHR14212. 1 hit.
PfamiPF06544. DUF1115. 1 hit.
PF08572. PRP3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03338 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPPRNTYEKG NPKRQNSPYY KPSFLRREET TNDEEKFQGH GLKTELHSAL
60 70 80 90 100
KSSNLNLIRR TYQTGENPYL SDPHDRGSSS RFNRRYERGL KFYQKGEISK
110 120 130 140 150
RIAQERTLQK QQEEEELKRK LKQEEDEKDK RKLIESGDLP NLELHEDKFL
160 170 180 190 200
LDLSKFKIYY DNNHGYEWWD TAYLDEKGEL MEKYDMNGTS PAEEKLAEDI
210 220 230 240 250
DEVDDDDDDE HPSIRYVAHP LPEKINEAKV SIKAYLTQHE RKRLRRNRRK
260 270 280 290 300
MAREAREIKI KLGLLPKPEP KVKLSNMMSV FENDQNITDP TAWEKVVKDQ
310 320 330 340 350
VDLRKRKHLE ENERRHEDAI KRRKEAVNMN VEKPTVYHCK VFQFKNLQNP
360 370 380 390 400
KIRFKLKMNS KELSLKGLCL RIRDDGPGII IVVGNEKSCK FYENLVMKRI
410 420 430 440 450
KWNEDFELHT NTGDIKMDMH NNSISKTWEG YLQDCKFKGW FMKVCNDQDS
460
LLRTLGQFDS EHFYSPVQT
Length:469
Mass (Da):55,870
Last modified:November 1, 1996 - v1
Checksum:iE977FD9F708BDD91
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U33050 Genomic DNA. Translation: AAB64932.1.
BK006938 Genomic DNA. Translation: DAA12307.1.
PIRiS69640.
RefSeqiNP_010761.3. NM_001180781.3.

Genome annotation databases

EnsemblFungiiYDR473C; YDR473C; YDR473C.
GeneIDi852084.
KEGGisce:YDR473C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U33050 Genomic DNA. Translation: AAB64932.1 .
BK006938 Genomic DNA. Translation: DAA12307.1 .
PIRi S69640.
RefSeqi NP_010761.3. NM_001180781.3.

3D structure databases

ProteinModelPortali Q03338.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32526. 53 interactions.
DIPi DIP-5266N.
IntActi Q03338. 27 interactions.
MINTi MINT-490402.
STRINGi 4932.YDR473C.

Proteomic databases

MaxQBi Q03338.
PaxDbi Q03338.
PRIDEi Q03338.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR473C ; YDR473C ; YDR473C .
GeneIDi 852084.
KEGGi sce:YDR473C.

Organism-specific databases

CYGDi YDR473c.
SGDi S000002881. PRP3.

Phylogenomic databases

eggNOGi NOG268901.
GeneTreei ENSGT00390000011497.
HOGENOMi HOG000205412.
InParanoidi Q03338.
KOi K12843.
OMAi NERRHED.
OrthoDBi EOG74XSH4.

Enzyme and pathway databases

BioCyci YEAST:G3O-30000-MONOMER.

Miscellaneous databases

NextBioi 970400.
PROi Q03338.

Gene expression databases

Genevestigatori Q03338.

Family and domain databases

InterProi IPR010541. DUF1115.
IPR013881. Pre-mRNA_splic_Prp3.
IPR027104. Prp3.
[Graphical view ]
PANTHERi PTHR14212. PTHR14212. 1 hit.
Pfami PF06544. DUF1115. 1 hit.
PF08572. PRP3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The yeast Prp3 protein is a U4/U6 snRNP protein necessary for integrity of the U4/U6 snRNP and the U4/U6.U5 tri-snRNP."
    Anthony J.G., Weidenhammer E.M., Woolford J.L. Jr.
    RNA 3:1143-1152(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP."
    Gottschalk A., Neubauer G., Banroques J., Mann M., Luehrmann R., Fabrizio P.
    EMBO J. 18:4535-4548(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy."
    Hacker I., Sander B., Golas M.M., Wolf E., Karagoz E., Kastner B., Stark H., Fabrizio P., Luhrmann R.
    Nat. Struct. Mol. Biol. 15:1206-1212(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, IDENTIFICATION IN THE U4/U5/U6 TRI-SNRNP COMPLEX, ELECTRON MICROSCOPY.

Entry informationi

Entry nameiPRP3_YEAST
AccessioniPrimary (citable) accession number: Q03338
Secondary accession number(s): D6VT97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3