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Q03337 (TRS31_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trafficking protein particle complex subunit 31

Short name=TRAPP subunit 31
Alternative name(s):
Transport protein particle 31 kDa subunit
Gene names
Name:TRS31
Ordered Locus Names:YDR472W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length283 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation. Ref.3 Ref.4 Ref.7 Ref.8

Subunit structure

Part of the multisubunit TRAPP (transport protein particle) I complex composed of BET3, BET5, TRS20, TRS23, TRS31 and TRS33. Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS85, TRS120 and TRS130. Part of the multisubunit TRAPP (transport protein particle) III complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33 and TRS85. Ref.3 Ref.4 Ref.7 Ref.8

Subcellular location

Golgi apparatuscis-Golgi network. Endoplasmic reticulum. Preautophagosomal structure Ref.4 Ref.8.

Miscellaneous

Present with 8350 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TRAPP small subunits family. BET3 subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BET3P3614913EBI-38770,EBI-3567
TRS20P383345EBI-38770,EBI-19468

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 283283Trafficking protein particle complex subunit 31
PRO_0000211585

Secondary structure

.............................. 283
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q03337 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 857BBA3E03423D42

FASTA28331,722
        10         20         30         40         50         60 
MSQRIIQPSA SDQQFPGKSD GYEYTVGPKQ AITSEASTTY IPSRIYSESL LFKRQEASLS 

        70         80         90        100        110        120 
AMAFLFQEMI SQLHRTCKTA GDFETKLSDY GHNIGIRLLE LLNFRASVSP SSLPRASAFL 

       130        140        150        160        170        180 
SQNESSSKLS NASNSPGMLA NSSTATSASA NERLQEKQTE SLSNYITKMR RRDLKILDIL 

       190        200        210        220        230        240 
QFIHGTLWSY LFNHVSDDLV KSSERDNEYM IVDNFPTLTQ FIPGENVSCE YFVCGIIKGF 

       250        260        270        280 
LFNAGFPCGV TAHRMPQGGH SQRTVYLIQF DRQVLDREGL RFG 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion."
Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.
EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE TRAPP II COMPLEX.
[4]"TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport."
Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S.
Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, FUNCTION OF THE TRAPP I COMPLEX, IDENTIFICATION IN THE TRAPP I COMPLEX, SUBCELLULAR LOCATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Molecular architecture of the TRAPPII complex and implications for vesicle tethering."
Yip C.K., Berscheminski J., Walz T.
Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
[8]"Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy."
Lynch-Day M.A., Bhandari D., Menon S., Huang J., Cai H., Bartholomew C.R., Brumell J.H., Ferro-Novick S., Klionsky D.J.
Proc. Natl. Acad. Sci. U.S.A. 107:7811-7816(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TRAPP III COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE TRAPP III COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U33050 Genomic DNA. Translation: AAB64914.1.
BK006938 Genomic DNA. Translation: DAA12306.1.
PIRS69639.
RefSeqNP_010760.3. NM_001180780.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3CUEX-ray3.70B/H/N/T1-283[»]
ProteinModelPortalQ03337.
SMRQ03337. Positions 55-282.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32525. 57 interactions.
DIPDIP-1709N.
IntActQ03337. 10 interactions.
MINTMINT-401389.
STRING4932.YDR472W.

Proteomic databases

PaxDbQ03337.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR472W; YDR472W; YDR472W.
GeneID852083.
KEGGsce:YDR472W.

Organism-specific databases

CYGDYDR472w.
SGDS000002880. TRS31.

Phylogenomic databases

eggNOGCOG5128.
GeneTreeENSGT00390000000976.
HOGENOMHOG000176758.
OrthoDBEOG7XSTS0.

Enzyme and pathway databases

BioCycYEAST:G3O-29999-MONOMER.

Gene expression databases

GenevestigatorQ03337.

Family and domain databases

InterProIPR024096. NO_sig/Golgi_transp_ligand-bd.
IPR016696. TRAPP-I_su5.
IPR007194. TRAPP_component.
[Graphical view]
PANTHERPTHR20902. PTHR20902. 1 hit.
PfamPF04051. TRAPP. 1 hit.
[Graphical view]
PIRSFPIRSF017479. TRAPP_I_complex_Trs31. 1 hit.
SUPFAMSSF111126. SSF111126. 2 hits.
ProtoNetSearch...

Other

EvolutionaryTraceQ03337.
NextBio970397.
PROQ03337.

Entry information

Entry nameTRS31_YEAST
AccessionPrimary (citable) accession number: Q03337
Secondary accession number(s): D6VT96
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references