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Q03333 (NEC2_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Neuroendocrine convertase 2
Short name=NEC 2
EC=3.4.21.94
Alternative name(s):
KEX2-like endoprotease 2
Prohormone convertase 2
Proprotein convertase 2
Short name=PC2
Gene names
Name:PCSK2
Synonyms:PC2
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length638 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Responsible for the release of glucagon from proglucagon in pancreatic A cells By similarity.

Catalytic activity

Release of protein hormones and neuropeptides from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

Subcellular location

Cytoplasmic vesicle › secretory vesicle. Note: Localized in the secretion granules.

Sequence similarities

Belongs to the peptidase S8 family. Furin subfamily.

Ontologies

Keywords
   Cellular componentCytoplasmic vesicle
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMCleavage on pair of basic residues
Disulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenttransport vesicle

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Propeptide26 – 10984 Potential
PRO_0000027069
Chain110 – 638529Neuroendocrine convertase 2
PRO_0000027070

Regions

Region122 – 415294Catalytic By similarity

Sites

Active site1671Charge relay system By similarity
Active site2081Charge relay system By similarity
Active site3841Charge relay system By similarity

Amino acid modifications

Glycosylation3751N-linked (GlcNAc...) Potential
Glycosylation5141N-linked (GlcNAc...) Potential
Glycosylation5241N-linked (GlcNAc...) Potential
Disulfide bond225 ↔ 376 By similarity
Disulfide bond317 ↔ 347 By similarity
Disulfide bond468 ↔ 494 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q03333 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 6654923420AC8B85

FASTA63870,452
        10         20         30         40         50         60 
MKGGCVSQWK AAAGLLFCVT VFASAERPVF TNHFLVELHK GGEEEARQVA AEHGFGVRKL 

        70         80         90        100        110        120 
PFAEGLYHFY HNGLAKAKRR RSLHHKQRLE RDPRVKRALQ QEGFDRKKRG YRDINEIDIN 

       130        140        150        160        170        180 
MNDPLFTKQW YLINTGQADG TPGLDLNVAE AWELGYTGKG VTIGIMDDGI DYLHPDLASN 

       190        200        210        220        230        240 
YNAEASYDFS SNDPYPYPRY TDDWFNSHGT RCAGEVSAAA NNNICGVGVA YSSKVAGIRM 

       250        260        270        280        290        300 
LDQPFMTDII EASSISHMPQ LIDIYSASWG PTDNGKTVDG PRELTLQAMA DGVNKGRGGK 

       310        320        330        340        350        360 
GSIYVWASGD GGSYDDCNCD GYASSMWTIS INSAINDGRT ALYDESCSST LASTFSNGRK 

       370        380        390        400        410        420 
RNPEAGVATT DLYGNCTLRH SGTSAAAPEA AGVFALALEA NLGLTWRDMQ HLTVLTSKRN 

       430        440        450        460        470        480 
QLHDEVHQWR RNGVGLEFNH LFGYGVLDAG AMVKMAKDWK TVPERFHCVG GSVQDPEKIP 

       490        500        510        520        530        540 
STGKLVLTLT TDACEGKENF VRYLEHVQAV ITVNATRRGD LNINMTSPMG TKSILLSRRP 

       550        560        570        580        590        600 
RDDDSKVGFD KWPFMTTHTW GEDARGTWTL ELGFVGSAPQ KGAVKEWTLM LHGSQSAPYI 

       610        620        630 
DQVVRDYQSK LAMSKKEELE EELDEAVERS LKSILGKH

« Hide

References

[1]"The cDNA structure of the porcine pro-hormone convertase PC2 and the comparative processing by PC1 and PC2 of the N-terminal glycopeptide segment of porcine POMC."
Seidah N.G., Fournier H., Boileau G., Benjannet S., Rondeau N., Chretien M.
FEBS Lett. 310:235-239(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pituitary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X68603 mRNA. Translation: CAA48593.1.
PIRS29244.
RefSeqNP_001004044.1. NM_001004044.1.
UniGeneSsc.109.

3D structure databases

ProteinModelPortalQ03333.
SMRQ03333. Positions 120-597.
ModBaseSearch...

Protein-protein interaction databases

STRING9823.ENSSSCP00000007546.

Protein family/group databases

MEROPSS08.073.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID445533.
KEGGssc:445533.

Organism-specific databases

CTD100533362.

Phylogenomic databases

eggNOGCOG4935.
HOGENOMHOG000192536.
HOVERGENHBG008705.
KOK01360.
OrthoDBEOG46MBJ1.

Family and domain databases

Gene3D3.40.50.200. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
[Graphical view]
PANTHERPTHR10795. PTHR10795. 1 hit.
PfamPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSPR00723. SUBTILISIN.
SUPFAMSSF49785. Gal_bind_like. 1 hit.
SSF52743. Pept_S8_S53. 1 hit.
SSF54897. Prot_inh_propept. 1 hit.
PROSITEPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNEC2_PIG
AccessionPrimary (citable) accession number: Q03333
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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