Reviewed, UniProtKB/Swiss-Prot Q03333 (NEC2_PIG)

Names and origin

Protein names

Recommended name:
    Neuroendocrine convertase 2
      Short name=NEC 2
    EC=3.4.21.94
Alternative name(s):
    Prohormone convertase 2
    Proprotein convertase 2
      Short name=PC2
    KEX2-like endoprotease 2

Gene names

Name:	PCSK2
Synonyms:	PC2

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	638 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues.
Catalytic activity	Release of protein hormones and neuropeptides from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.
Subcellular location	Cytoplasmic vesicle › secretory vesicle. Note: Localized in the secretion granules.
Sequence similarities	Belongs to the peptidase S8 family. Furin subfamily.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Cellular component	secretory granule Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Propeptide	26 – 109	84	Potential		PRO_0000027069
	Chain	110 – 638	529	Neuroendocrine convertase 2		PRO_0000027070
Regions
	Region	122 – 415	294	Catalytic By similarity
Sites
	Active site	167	1	Charge relay system By similarity
	Active site	208	1	Charge relay system By similarity
	Active site	384	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	375	1	N-linked (GlcNAc...) Potential
	Glycosylation	514	1	N-linked (GlcNAc...) Potential
	Glycosylation	524	1	N-linked (GlcNAc...) Potential
	Disulfide bond	225 ↔ 376		By similarity
	Disulfide bond	317 ↔ 347		By similarity
	Disulfide bond	468 ↔ 494		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q03333-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 6654923420AC8B85	FASTA	638	70,452
	10 20 30 40 50 60 MKGGCVSQWK AAAGLLFCVT VFASAERPVF TNHFLVELHK GGEEEARQVA AEHGFGVRKL 70 80 90 100 110 120 PFAEGLYHFY HNGLAKAKRR RSLHHKQRLE RDPRVKRALQ QEGFDRKKRG YRDINEIDIN 130 140 150 160 170 180 MNDPLFTKQW YLINTGQADG TPGLDLNVAE AWELGYTGKG VTIGIMDDGI DYLHPDLASN 190 200 210 220 230 240 YNAEASYDFS SNDPYPYPRY TDDWFNSHGT RCAGEVSAAA NNNICGVGVA YSSKVAGIRM 250 260 270 280 290 300 LDQPFMTDII EASSISHMPQ LIDIYSASWG PTDNGKTVDG PRELTLQAMA DGVNKGRGGK 310 320 330 340 350 360 GSIYVWASGD GGSYDDCNCD GYASSMWTIS INSAINDGRT ALYDESCSST LASTFSNGRK 370 380 390 400 410 420 RNPEAGVATT DLYGNCTLRH SGTSAAAPEA AGVFALALEA NLGLTWRDMQ HLTVLTSKRN 430 440 450 460 470 480 QLHDEVHQWR RNGVGLEFNH LFGYGVLDAG AMVKMAKDWK TVPERFHCVG GSVQDPEKIP 490 500 510 520 530 540 STGKLVLTLT TDACEGKENF VRYLEHVQAV ITVNATRRGD LNINMTSPMG TKSILLSRRP 550 560 570 580 590 600 RDDDSKVGFD KWPFMTTHTW GEDARGTWTL ELGFVGSAPQ KGAVKEWTLM LHGSQSAPYI 610 620 630 DQVVRDYQSK LAMSKKEELE EELDEAVERS LKSILGKH

References

[1]

"The cDNA structure of the porcine pro-hormone convertase PC2 and the comparative processing by PC1 and PC2 of the N-terminal glycopeptide segment of porcine POMC." Seidah N.G., Fournier H., Boileau G., Benjannet S., Rondeau N., Chretien M. FEBS Lett. 310:235-239(1992) [PubMed: 1397279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pituitary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X68603 mRNA. Translation: CAA48593.1.
PIR	S29244.
RefSeq	NP_001004044.1.
UniGene	Ssc.109
3D structure databases
SMR	Q03333. Positions 30-101, 120-597.
ModBase	Search...
Protein family/group databases
MEROPS	S08.073.
Genome annotation databases
Ensembl	ENSSSCT00000007755; ENSSSCP00000007546; ENSSSCG00000007083; Sus scrofa. [Genome view]
GeneID	445533.
KEGG	ssc:445533.
Organism-specific databases
CTD	445533.
Phylogenomic databases
HOVERGEN	Q03333.
Enzyme and pathway databases
BRENDA	3.4.21.94. 249.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR000209. Peptidase_S8/S53. IPR015500. Peptidase_S8_subtilisin-rel. IPR009020. Prot_inh_propept. IPR002884. PrprotnconvertsP. [Graphical view]
Gene3D	G3DSA:3.40.50.200. Pept_S8_S53. 1 hit.
PANTHER	PTHR10795. SubtilSerProt. 1 hit.
Pfam	PF01483. P_proprotein. 1 hit. PF00082. Peptidase_S8. 1 hit. [Graphical view]
PRINTS	PR00723. SUBTILISIN.
PROSITE	PS00136. SUBTILASE_ASP. 1 hit. PS00137. SUBTILASE_HIS. 1 hit. PS00138. SUBTILASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NEC2_PIG

Accession

Primary (citable) accession number: Q03333

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 19, 2010
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)