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Protein

Mitochondrial fusion and transport protein UGO1

Gene

UGO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for mitochondrial fusion as well as normal mitochondrial morphology by bridging the essential interaction between FZO1 and MGM1. May coordinate fusion of inner and outer membranes during mitochondrial fusion.2 Publications

GO - Biological processi

  • mitochondrial fusion Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29997-MONOMER.

Protein family/group databases

TCDBi9.B.25.1.1. the mitochondrial inner/outer membrane fusion (mmf) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial fusion and transport protein UGO1
Gene namesi
Name:UGO1Imported
Ordered Locus Names:YDR470C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR470C.
SGDiS000002878. UGO1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 293293CytoplasmicSequence analysisAdd
BLAST
Transmembranei294 – 31421Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini315 – 502188Mitochondrial intermembraneSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of mitochondrial outer membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Mitochondrial fusion and transport protein UGO1PRO_0000270985Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ03327.
PeptideAtlasiQ03327.

Interactioni

Subunit structurei

Interacts with FZO1 through its cytoplasmic domain and with MGM1 through its mitochondrial intermembrane space domain.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FZO1P382972EBI-32955,EBI-20900
MGM1P322662EBI-32955,EBI-10865

Protein-protein interaction databases

BioGridi32523. 54 interactions.
IntActiQ03327. 5 interactions.
MINTiMINT-2783849.

Structurei

3D structure databases

ProteinModelPortaliQ03327.
SMRiQ03327. Positions 116-142.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati288 – 38396SolcarSequence analysisAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 294294Binds FZO11 PublicationAdd
BLAST
Regioni312 – 502191Binds MGM11 PublicationAdd
BLAST

Sequence similaritiesi

Contains 1 Solcar repeat.Sequence analysis

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000141868.
InParanoidiQ03327.
KOiK17967.
OMAiGVWRANN.
OrthoDBiEOG7H1JZ5.

Family and domain databases

Gene3Di1.50.40.10. 2 hits.
InterProiIPR023395. Mt_carrier_dom.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 2 hits.

Sequencei

Sequence statusi: Complete.

Q03327-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNNNVTEAT SRAQIRPYYD PDSFNAGYSA VFKPDEGVVD PHGYTIASKL
60 70 80 90 100
NVINSSPTTK RMANALFKSS PMKKLSNSVN DGLSLEGSNG EITGLNNFEW
110 120 130 140 150
AELVNIQKWR KIFEQLLDMF FRKYFQLLIQ QPFDVARLLI QVGEFKIFKT
160 170 180 190 200
TVDTNKPQAP IILRDEEGDG AAREGEEDAY DEEEIDFFPI ERKIAEANST
210 220 230 240 250
APIMAEETDH SHHEPTDISL TIAPQSLHTI DVINALFDQE GIRGLWKANN
260 270 280 290 300
TTFIYNFLSL SIDTWFTGLL SSFLGVPDPY FMEVINSPDI SKSFILALGA
310 320 330 340 350
GVFTSIILLP VDLIRTRLIV TSFKKKKNVK TDGKNMVTNT RSLRQLIRCW
360 370 380 390 400
SWRKNGVSIP LDMWSLTILQ SINNSFFNKL FDLVIYNQFH IEKYSQTVMY
410 420 430 440 450
NTMKFFSKSL ELFIKLPLEN LLRRCQLNYL LNDQRLSFKV DSTELIVKPK
460 470 480 490 500
KYNGIWDVIR NNSNTNRGQL WNGWKVGVIS LICGYGLQMM NKVDINMEQE

KF
Length:502
Mass (Da):57,543
Last modified:July 27, 2011 - v3
Checksum:i6417C4730DACD8F6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1141E → G in AAB64924 (PubMed:9169867).Curated
Sequence conflicti114 – 1141E → G no nucleotide entry (PubMed:10930523).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA. Translation: AAB64924.2.
BK006938 Genomic DNA. Translation: DAA12304.2.
PIRiS69637.
RefSeqiNP_010758.3. NM_001180778.2.

Genome annotation databases

EnsemblFungiiYDR470C; YDR470C; YDR470C.
GeneIDi852081.
KEGGisce:YDR470C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA. Translation: AAB64924.2.
BK006938 Genomic DNA. Translation: DAA12304.2.
PIRiS69637.
RefSeqiNP_010758.3. NM_001180778.2.

3D structure databases

ProteinModelPortaliQ03327.
SMRiQ03327. Positions 116-142.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32523. 54 interactions.
IntActiQ03327. 5 interactions.
MINTiMINT-2783849.

Protein family/group databases

TCDBi9.B.25.1.1. the mitochondrial inner/outer membrane fusion (mmf) family.

Proteomic databases

MaxQBiQ03327.
PeptideAtlasiQ03327.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR470C; YDR470C; YDR470C.
GeneIDi852081.
KEGGisce:YDR470C.

Organism-specific databases

EuPathDBiFungiDB:YDR470C.
SGDiS000002878. UGO1.

Phylogenomic databases

HOGENOMiHOG000141868.
InParanoidiQ03327.
KOiK17967.
OMAiGVWRANN.
OrthoDBiEOG7H1JZ5.

Enzyme and pathway databases

BioCyciYEAST:G3O-29997-MONOMER.

Miscellaneous databases

PROiQ03327.

Family and domain databases

Gene3Di1.50.40.10. 2 hits.
InterProiIPR023395. Mt_carrier_dom.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288cImported.
  2. "The yeast mitochondrial transport proteins: new sequences and consensus residues, lack of direct relation between consensus residues and transmembrane helices, expression patterns of the transport protein genes, and protein-protein interactions with other proteins."
    Belenkiy R., Haefele A., Eisen M.B., Wohlrab H.
    Biochim. Biophys. Acta 1467:207-218(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 114.
  3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 114.
    Strain: ATCC 204508 / S288c.
  4. "UGO1 encodes an outer membrane protein required for mitochondrial fusion."
    Sesaki H., Jensen R.E.
    J. Cell Biol. 152:1123-1134(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY.
  5. "The intramitochondrial dynamin-related GTPase, Mgm1p, is a component of a protein complex that mediates mitochondrial fusion."
    Wong E.D., Wagner J.A., Scott S.V., Okreglak V., Holewinske T.J., Cassidy-Stone A., Nunnari J.
    J. Cell Biol. 160:303-311(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FZO1 AND MGM1.
  6. "Mgm1p, a dynamin-related GTPase, is essential for fusion of the mitochondrial outer membrane."
    Sesaki H., Southard S.M., Yaffe M.P., Jensen R.E.
    Mol. Biol. Cell 14:2342-2356(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FZO1 AND MGM1.
  7. Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: ATCC 76625 / YPH499.
  8. "Ugo1p links the Fzo1p and Mgm1p GTPases for mitochondrial fusion."
    Sesaki H., Jensen R.E.
    J. Biol. Chem. 279:28298-28303(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH FZO1 AND MGM1.
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUGO1_YEAST
AccessioniPrimary (citable) accession number: Q03327
Secondary accession number(s): D6VT94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.