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Protein

T-SNARE affecting a late Golgi compartment protein 1

Gene

TLG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SNARE protein (of Qc type) involved in membrane fusion probably in retrograde traffic of cytosolic double-membrane vesicles derived from both, early and possibly late endosomes/PVC (prevacuolar compartment) back to the trans-Golgi network (TGN or late Golgi). It has been reported to function both as a (target membrane) t-SNARE and as a (vesicle) v-SNARE. Upon vesicle tethering to the target membrane, which requires additional proteins, a SNARE-pin is formed. This is a very stable 4 parallel alpha-helical coil bundle consisting of 4 SNARE domains (usually one of each type: Qa, Qb, Qc, and R), of which at least one is anchored in the opposite membrane. The formation of the SNARE-pin is believed to bring the two membranes in close proximity and to provide the energy to drive membrane fusion. Through its interaction with the VFT (or GARP) complex, it may also contribute to vesicle recognition specificity and tethering. Regulation of SNARE-pin formation also seems to depend on the phosphorylation state of the protein, phosphorylation by TPK1 causing inhibition and dephosphorylation by SIT4 activation.6 Publications

GO - Molecular functioni

  • SNAP receptor activity Source: SGD
  • SNARE binding Source: GO_Central

GO - Biological processi

  • endocytosis Source: SGD
  • intracellular protein transport Source: GO_Central
  • vesicle docking Source: GO_Central
  • vesicle fusion Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29995-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
T-SNARE affecting a late Golgi compartment protein 1
Alternative name(s):
Syntaxin TLG1
Gene namesi
Name:TLG1
Ordered Locus Names:YDR468C
ORF Names:D8035.11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR468C.
SGDiS000002876. TLG1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 203CytoplasmicSequence analysisAdd BLAST203
Transmembranei204 – 224Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21

GO - Cellular componenti

  • early endosome membrane Source: UniProtKB-SubCell
  • endosome Source: SGD
  • integral component of membrane Source: GO_Central
  • late endosome membrane Source: UniProtKB-SubCell
  • SNARE complex Source: SGD
  • trans-Golgi network Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31T → A: Results in an aktivated t-SNARE that confers endocytosis, but not exocytosis. 1 Publication1
Mutagenesisi205 – 206CC → SS or LL: Not palmitoylated, rapidly degraded in a TUL1-dependent manner. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002102771 – 224T-SNARE affecting a late Golgi compartment protein 1Add BLAST224

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei31Phosphothreonine; by PKA1 Publication1
Cross-linki183Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Lipidationi205S-palmitoyl cysteine1 Publication1
Lipidationi206S-palmitoyl cysteine1 Publication1

Post-translational modificationi

Phosphorylated at Thr-31 by TPK1 and dephosphorylated by SIT4.1 Publication
Palmitoylated by SWF1, which prevents its recognition and ubiquitination by TUL1 and its subsequent degradation.1 Publication

Keywords - PTMi

Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ03322.
PRIDEiQ03322.

PTM databases

iPTMnetiQ03322.
SwissPalmiQ03322.

Interactioni

Subunit structurei

Interacts in a SNARE-pin with the SNAREs TLG2 (Qa), VTI1 (Qb), and SNC1 or SNC2 (R). Interacts with VPS51 of the VFT (or GARP) complex.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
VPS51P361166EBI-38705,EBI-26352

GO - Molecular functioni

  • SNAP receptor activity Source: SGD
  • SNARE binding Source: GO_Central

Protein-protein interaction databases

BioGridi32521. 59 interactors.
DIPiDIP-2058N.
IntActiQ03322. 7 interactors.
MINTiMINT-490335.

Structurei

Secondary structure

1224
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 28Combined sources22
Helixi38 – 63Combined sources26
Helixi71 – 92Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C5IX-ray2.30T1-101[»]
2C5JX-ray2.10A/B1-95[»]
2C5KX-ray2.05T1-95[»]
ProteinModelPortaliQ03322.
SMRiQ03322.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03322.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini132 – 194t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 106Interaction with VPS511 PublicationAdd BLAST106

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili35 – 101Sequence analysisAdd BLAST67

Sequence similaritiesi

Belongs to the syntaxin family.Curated
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000248369.
InParanoidiQ03322.
KOiK08499.
OMAiNPFQEQM.
OrthoDBiEOG092C5GHY.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03322-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNSEDPFQQ VVKDTKEQLN RINNYITRHN TAGDDDQEEE IQDILKDVEE
60 70 80 90 100
TIVDLDRSII VMKRDENEDV SGREAQVKNI KQQLDALKLR FDRRIQESTQ
110 120 130 140 150
TTIPLEETVE NSTLNTSMAE NNDGGMSNPF QEQMLREQDV HLDGIHKTMQ
160 170 180 190 200
NLHIQAQTMG DELENQGQLL DNMDEGMDGV VNKLARGRRQ LEWVYEKNKE
210 220
KYDDCCIGLL IVVLIVLLVL AFIA
Length:224
Mass (Da):25,817
Last modified:November 1, 1996 - v1
Checksum:iB33251C18645EF74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA. Translation: AAB64925.1.
AY558178 Genomic DNA. Translation: AAS56504.1.
BK006938 Genomic DNA. Translation: DAA12302.1.
PIRiS69635.
RefSeqiNP_010756.3. NM_001180776.3.

Genome annotation databases

EnsemblFungiiYDR468C; YDR468C; YDR468C.
GeneIDi852079.
KEGGisce:YDR468C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA. Translation: AAB64925.1.
AY558178 Genomic DNA. Translation: AAS56504.1.
BK006938 Genomic DNA. Translation: DAA12302.1.
PIRiS69635.
RefSeqiNP_010756.3. NM_001180776.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C5IX-ray2.30T1-101[»]
2C5JX-ray2.10A/B1-95[»]
2C5KX-ray2.05T1-95[»]
ProteinModelPortaliQ03322.
SMRiQ03322.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32521. 59 interactors.
DIPiDIP-2058N.
IntActiQ03322. 7 interactors.
MINTiMINT-490335.

PTM databases

iPTMnetiQ03322.
SwissPalmiQ03322.

Proteomic databases

MaxQBiQ03322.
PRIDEiQ03322.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR468C; YDR468C; YDR468C.
GeneIDi852079.
KEGGisce:YDR468C.

Organism-specific databases

EuPathDBiFungiDB:YDR468C.
SGDiS000002876. TLG1.

Phylogenomic databases

HOGENOMiHOG000248369.
InParanoidiQ03322.
KOiK08499.
OMAiNPFQEQM.
OrthoDBiEOG092C5GHY.

Enzyme and pathway databases

BioCyciYEAST:G3O-29995-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ03322.
PROiQ03322.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
SMARTiSM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTLG1_YEAST
AccessioniPrimary (citable) accession number: Q03322
Secondary accession number(s): D6VT92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.