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Q03322

- TLG1_YEAST

UniProt

Q03322 - TLG1_YEAST

Protein

T-SNARE affecting a late Golgi compartment protein 1

Gene

TLG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    SNARE protein (of Qc type) involved in membrane fusion probably in retrograde traffic of cytosolic double-membrane vesicles derived from both, early and possibly late endosomes/PVC (prevacuolar compartment) back to the trans-Golgi network (TGN or late Golgi). It has been reported to function both as a (target membrane) t-SNARE and as a (vesicle) v-SNARE. Upon vesicle tethering to the target membrane, which requires additional proteins, a SNARE-pin is formed. This is a very stable 4 parallel alpha-helical coil bundle consisting of 4 SNARE domains (usually one of each type: Qa, Qb, Qc, and R), of which at least one is anchored in the opposite membrane. The formation of the SNARE-pin is believed to bring the two membranes in close proximity and to provide the energy to drive membrane fusion. Through its interaction with the VFT (or GARP) complex, it may also contribute to vesicle recognition specificity and tethering. Regulation of SNARE-pin formation also seems to depend on the phosphorylation state of the protein, phosphorylation by TPK1 causing inhibition and dephosphorylation by SIT4 activation.6 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. SNAP receptor activity Source: SGD

    GO - Biological processi

    1. endocytosis Source: SGD
    2. Golgi vesicle transport Source: InterPro
    3. intracellular protein transport Source: InterPro
    4. vesicle fusion Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29995-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    T-SNARE affecting a late Golgi compartment protein 1
    Alternative name(s):
    Syntaxin TLG1
    Gene namesi
    Name:TLG1
    Ordered Locus Names:YDR468C
    ORF Names:D8035.11
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR468c.
    SGDiS000002876. TLG1.

    Subcellular locationi

    GO - Cellular componenti

    1. early endosome membrane Source: UniProtKB-SubCell
    2. endosome Source: SGD
    3. integral component of membrane Source: UniProtKB-KW
    4. late endosome membrane Source: UniProtKB-SubCell
    5. SNARE complex Source: SGD
    6. trans-Golgi network Source: SGD

    Keywords - Cellular componenti

    Endosome, Golgi apparatus, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi31 – 311T → A: Results in an aktivated t-SNARE that confers endocytosis, but not exocytosis. 2 Publications
    Mutagenesisi205 – 2062CC → SS or LL: Not palmitoylated, rapidly degraded in a TUL1-dependent manner. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 224224T-SNARE affecting a late Golgi compartment protein 1PRO_0000210277Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei31 – 311Phosphothreonine; by PKA1 Publication
    Lipidationi205 – 2051S-palmitoyl cysteine1 Publication
    Lipidationi206 – 2061S-palmitoyl cysteine1 Publication

    Post-translational modificationi

    Phosphorylated at Thr-31 by TPK1 and dephosphorylated by SIT4.1 Publication
    Palmitoylated by SWF1, which prevents its recognition and ubiquitination by TUL1 and its subsequent degradation.1 Publication

    Keywords - PTMi

    Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ03322.
    PaxDbiQ03322.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03322.

    Interactioni

    Subunit structurei

    Interacts in a SNARE-pin with the SNAREs TLG2 (Qa), VTI1 (Qb), and SNC1 or SNC2 (R). Interacts with VPS51 of the VFT (or GARP) complex.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    VPS51P361166EBI-38705,EBI-26352

    Protein-protein interaction databases

    BioGridi32521. 49 interactions.
    DIPiDIP-2058N.
    IntActiQ03322. 7 interactions.
    MINTiMINT-490335.
    STRINGi4932.YDR468C.

    Structurei

    Secondary structure

    1
    224
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 2822
    Helixi38 – 6326
    Helixi71 – 9222

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2C5IX-ray2.30T1-101[»]
    2C5JX-ray2.10A/B1-95[»]
    2C5KX-ray2.05T1-95[»]
    ProteinModelPortaliQ03322.
    SMRiQ03322. Positions 6-94.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ03322.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 203203CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei204 – 22421Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini132 – 19463t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 106106Interaction with VPS51Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili35 – 10167Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the syntaxin family.Curated
    Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG255019.
    HOGENOMiHOG000248369.
    KOiK08499.
    OMAiRRQLEWV.
    OrthoDBiEOG71GB6W.

    Family and domain databases

    InterProiIPR015260. Syntaxin-6_N.
    IPR006012. Syntaxin/epimorphin_CS.
    IPR010989. t-SNARE.
    IPR000727. T_SNARE_dom.
    [Graphical view]
    PfamiPF05739. SNARE. 1 hit.
    PF09177. Syntaxin-6_N. 1 hit.
    [Graphical view]
    SMARTiSM00397. t_SNARE. 1 hit.
    [Graphical view]
    SUPFAMiSSF47661. SSF47661. 1 hit.
    PROSITEiPS00914. SYNTAXIN. 1 hit.
    PS50192. T_SNARE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03322-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNSEDPFQQ VVKDTKEQLN RINNYITRHN TAGDDDQEEE IQDILKDVEE    50
    TIVDLDRSII VMKRDENEDV SGREAQVKNI KQQLDALKLR FDRRIQESTQ 100
    TTIPLEETVE NSTLNTSMAE NNDGGMSNPF QEQMLREQDV HLDGIHKTMQ 150
    NLHIQAQTMG DELENQGQLL DNMDEGMDGV VNKLARGRRQ LEWVYEKNKE 200
    KYDDCCIGLL IVVLIVLLVL AFIA 224
    Length:224
    Mass (Da):25,817
    Last modified:November 1, 1996 - v1
    Checksum:iB33251C18645EF74
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33050 Genomic DNA. Translation: AAB64925.1.
    AY558178 Genomic DNA. Translation: AAS56504.1.
    BK006938 Genomic DNA. Translation: DAA12302.1.
    PIRiS69635.
    RefSeqiNP_010756.3. NM_001180776.3.

    Genome annotation databases

    EnsemblFungiiYDR468C; YDR468C; YDR468C.
    GeneIDi852079.
    KEGGisce:YDR468C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33050 Genomic DNA. Translation: AAB64925.1 .
    AY558178 Genomic DNA. Translation: AAS56504.1 .
    BK006938 Genomic DNA. Translation: DAA12302.1 .
    PIRi S69635.
    RefSeqi NP_010756.3. NM_001180776.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2C5I X-ray 2.30 T 1-101 [» ]
    2C5J X-ray 2.10 A/B 1-95 [» ]
    2C5K X-ray 2.05 T 1-95 [» ]
    ProteinModelPortali Q03322.
    SMRi Q03322. Positions 6-94.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32521. 49 interactions.
    DIPi DIP-2058N.
    IntActi Q03322. 7 interactions.
    MINTi MINT-490335.
    STRINGi 4932.YDR468C.

    Proteomic databases

    MaxQBi Q03322.
    PaxDbi Q03322.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR468C ; YDR468C ; YDR468C .
    GeneIDi 852079.
    KEGGi sce:YDR468C.

    Organism-specific databases

    CYGDi YDR468c.
    SGDi S000002876. TLG1.

    Phylogenomic databases

    eggNOGi NOG255019.
    HOGENOMi HOG000248369.
    KOi K08499.
    OMAi RRQLEWV.
    OrthoDBi EOG71GB6W.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29995-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q03322.
    NextBioi 970385.
    PROi Q03322.

    Gene expression databases

    Genevestigatori Q03322.

    Family and domain databases

    InterProi IPR015260. Syntaxin-6_N.
    IPR006012. Syntaxin/epimorphin_CS.
    IPR010989. t-SNARE.
    IPR000727. T_SNARE_dom.
    [Graphical view ]
    Pfami PF05739. SNARE. 1 hit.
    PF09177. Syntaxin-6_N. 1 hit.
    [Graphical view ]
    SMARTi SM00397. t_SNARE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47661. SSF47661. 1 hit.
    PROSITEi PS00914. SYNTAXIN. 1 hit.
    PS50192. T_SNARE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Two syntaxin homologues in the TGN/endosomal system of yeast."
      Holthuis J.C.M., Nichols B.J., Dhruvakumar S., Pelham H.R.B.
      EMBO J. 17:113-126(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SNARE PROTEINS, SUBCELLULAR LOCATION.
    5. "A role for Tlg1p in the transport of proteins within the Golgi apparatus of Saccharomyces cerevisiae."
      Coe J.G., Lim A.C., Xu J., Hong W.
      Mol. Biol. Cell 10:2407-2423(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SNARE PROTEINS AND SEC17, STRAIN SPECIFIC EFFECTS, SUBCELLULAR LOCATION.
    6. "A genomic perspective on membrane compartment organization."
      Bock J.B., Matern H.T., Peden A.A., Scheller R.H.
      Nature 409:839-841(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLASSIFICATION.
    7. "An effector of Ypt6p binds the SNARE Tlg1p and mediates selective fusion of vesicles with late Golgi membranes."
      Siniossoglou S., Pelham H.R.B.
      EMBO J. 20:5991-5998(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH VFT COMPLEX.
    8. "A t-SNARE of the endocytic pathway must be activated for fusion."
      Paumet F., Brugger B., Parlati F., McNew J.A., Sollner T.H., Rothman J.E.
      J. Cell Biol. 155:961-968(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION SPECIFICITY WITH SNARE PROTEINS.
    9. "t-SNARE phosphorylation regulates endocytosis in yeast."
      Gurunathan S., Marash M., Weinberger A., Gerst J.E.
      Mol. Biol. Cell 13:1594-1607(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT THR-31, MUTAGENESIS OF THR-31.
    10. "Vps51p links the VFT complex to the SNARE Tlg1p."
      Siniossoglou S., Pelham H.R.B.
      J. Biol. Chem. 277:48318-48324(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH VPS51.
    11. "Vps51p mediates the association of the GARP (Vps52/53/54) complex with the late Golgi t-SNARE Tlg1p."
      Conibear E., Cleck J.N., Stevens T.H.
      Mol. Biol. Cell 14:1610-1623(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, VFT COMPLEX IN RETROGRADE TRAFFIC FROM EARLY/LATE ENDOSOME TO TGN.
    12. "A complete set of SNAREs in yeast."
      Burri L., Lithgow T.
      Traffic 5:45-52(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    13. "Swf1-dependent palmitoylation of the SNARE Tlg1 prevents its ubiquitination and degradation."
      Valdez-Taubas J., Pelham H.R.B.
      EMBO J. 24:2524-2532(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PALMITOYLATION AT CYS-205 AND CYS-206, MUTAGENESIS OF 205-CYS-CYS-206.
    14. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTLG1_YEAST
    AccessioniPrimary (citable) accession number: Q03322
    Secondary accession number(s): D6VT92
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3