Q03321 (AROA_AERSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:

aroA

Organism

Aeromonas salmonicida

Taxonomic identifier

645 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer.
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.
Sequence caution	The sequence AAA21937.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000088214

Sequences

	Sequence		Length	Mass (Da)
	Q03321 [UniParc]. Last modified October 25, 2004. Version 4. Checksum: 506242A7C6569DBC	FASTA	427	46,152
	10 20 30 40 50 60 MNSLRLEPIS RVAGEVNLPG SKSVSNRALL LAALARGTTR LTNLLDSDDI RHMLAALTQL 70 80 90 100 110 120 GVKYKLSADK TECTVHGLGR SFAVSAPVNL FLGNAGTAMR PLCAALCLGS GEYMLGGEPR 130 140 150 160 170 180 MEERPIGHLV DCLALKGAHI QYLKKDGYPP LVVDAKGLWG GDVHVDGSVS SQFLTAFLMA 190 200 210 220 230 240 APAMAPVIPR IHIKGELVSK PYIDITLHIM NSSGVVIEHD NYKLFYIKGN QSIVSPGDFL 250 260 270 280 290 300 VEGDASSASY FLAAGAIKGK VRVTGIGKHS IGDIHFADVL ERMGARITWG DDFIEAEQGP 310 320 330 340 350 360 LHGVDMDMNH IPDXAMTIAX VALFAEGPTS IRNIYNWRVK ETDRLHAMAT DVRKLGVSEE 370 380 390 400 410 420 GDLHYITVTP PTQLKHAEID TYKHHRIAMC FSLVALSDIA VTINDPGCTS KTFPDYFDKL ASVSQAV

References

[1]

"An aromatic-dependent mutant of the fish pathogen Aeromonas salmonicida is attenuated in fish and is effective as a live vaccine against the salmonid disease furunculosis." Vaughan L.M., Smith P.R., Foster T.J. Infect. Immun. 61:2172-2181(1993) [PubMed: 8478107] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	L05002 Genomic DNA. Translation: AAA21937.1. Sequence problems.
PIR	I39539.
3D structure databases
ProteinModelPortal	Q03321.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00210. EPSP_synth. Divergent sequence. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_AERSA

Accession

Primary (citable) accession number: Q03321

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 25, 2004
Last modified:	June 28, 2011
This is version 75 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program