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Protein

Cholinesterase

Gene

Bche

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.2 Publications

Catalytic activityi

An acylcholine + H2O = choline + a carboxylate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei227Acyl-ester intermediatePROSITE-ProRule annotation1
Active sitei354Charge relay systemBy similarity1
Active sitei467Charge relay systemBy similarity1

GO - Molecular functioni

  • acetylcholinesterase activity Source: UniProtKB
  • choline binding Source: Ensembl
  • cholinesterase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

ReactomeiR-MMU-422085. Synthesis, secretion, and deacylation of Ghrelin.
SABIO-RKQ03311.

Protein family/group databases

ESTHERimouse-BCHE. BCHE.
MEROPSiS09.980.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholinesterase (EC:3.1.1.8)
Alternative name(s):
Acylcholine acylhydrolase
Butyrylcholine esterase
Choline esterase II
Pseudocholinesterase
Gene namesi
Name:Bche
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:894278. Bche.

Subcellular locationi

GO - Cellular componenti

  • blood microparticle Source: MGI
  • endoplasmic reticulum Source: MGI
  • endoplasmic reticulum lumen Source: Ensembl
  • extracellular space Source: GO_Central
  • membrane Source: Ensembl
  • nuclear envelope lumen Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

No visible phenotype; due to the presence of other cholinesterases. Hypersensitive to acetylcholinesterase inhibitors, such as huperzine and donepezil. Treatment with the acetylcholinesterase inhibitor donepezil causes convulsions and death within 3 hours of dosing.2 Publications

Chemistry databases

ChEMBLiCHEMBL2528.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Add BLAST29
ChainiPRO_000000861430 – 603CholinesteraseAdd BLAST574

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi86N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi94 ↔ 121By similarity
Glycosylationi135N-linked (GlcNAc...)Sequence analysis1
Modified residuei227PhosphoserineBy similarity1
Glycosylationi270N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi281 ↔ 292By similarity
Glycosylationi370N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi429 ↔ 548By similarity
Glycosylationi484N-linked (GlcNAc...)Sequence analysis1
Glycosylationi510N-linked (GlcNAc...)Sequence analysis1
Glycosylationi515N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi600InterchainBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ03311.
PaxDbiQ03311.
PeptideAtlasiQ03311.
PRIDEiQ03311.

PTM databases

PhosphoSitePlusiQ03311.

Expressioni

Tissue specificityi

Present in most cells except erythrocytes.

Gene expression databases

BgeeiENSMUSG00000027792.
CleanExiMM_BCHE.
ExpressionAtlasiQ03311. baseline and differential.
GenevisibleiQ03311. MM.

Interactioni

Subunit structurei

Homotetramer; disulfide-linked. Dimer of dimers (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029367.

Chemistry databases

BindingDBiQ03311.

Structurei

3D structure databases

ProteinModelPortaliQ03311.
SMRiQ03311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni145 – 146Substrate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4389. Eukaryota.
COG2272. LUCA.
GeneTreeiENSGT00760000118946.
HOGENOMiHOG000091866.
HOVERGENiHBG008839.
InParanoidiQ03311.
KOiK01050.
OMAiFLGSEMW.
OrthoDBiEOG091G0I4G.
TreeFamiTF315470.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00878. CHOLNESTRASE.
ProDomiPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03311-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTQHTKVTQ THFLLWILLL CMPFGKSHTE EDFIITTKTG RVRGLSMPVL
60 70 80 90 100
GGTVTAFLGI PYAQPPLGSL RFKKPQPLNK WPDIHNATQY ANSCYQNIDQ
110 120 130 140 150
AFPGFQGSEM WNPNTNLSED CLYLNVWIPV PKPKNATVMV WIYGGGFQTG
160 170 180 190 200
TSSLPVYDGK FLARVERVIV VSMNYRVGAL GFLAFPGNPD APGNMGLFDQ
210 220 230 240 250
QLALQWVQRN IAAFGGNPKS ITIFGESAGA ASVSLHLLCP QSYPLFTRAI
260 270 280 290 300
LESGSSNAPW AVKHPEEARN RTLTLAKFTG CSKENEMEMI KCLRSKDPQE
310 320 330 340 350
ILRNERFVLP SDSILSINFG PTVDGDFLTD MPHTLLQLGK VKKAQILVGV
360 370 380 390 400
NKDEGTAFLV YGAPGFSKDN DSLITRKEFQ EGLNMYFPGV SRLGKEAVLF
410 420 430 440 450
YYVDWLGEQS PEVYRDALDD VIGDYNIICP ALEFTKKFAE LENNAFFYFF
460 470 480 490 500
EHRSSKLPWP EWMGVMHGYE IEFVFGLPLG RRVNYTRAEE IFSRSIMKTW
510 520 530 540 550
ANFAKYGHPN GTQGNSTMWP VFTSTEQKYL TLNTEKSKIY SKLRAPQCQF
560 570 580 590 600
WRLFFPKVLE MTGDIDETEQ EWKAGFHRWS NYMMDWQNQF NDYTSKKESC

TAL
Length:603
Mass (Da):68,462
Last modified:July 27, 2011 - v2
Checksum:i2CB79C46797B3713
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti129P → R in AAA37328 (PubMed:2400605).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M99492 mRNA. Translation: AAA37328.1.
AK050337 mRNA. Translation: BAC34196.1.
CH466547 Genomic DNA. Translation: EDL15482.1.
CCDSiCCDS17411.1.
PIRiS70849.
RefSeqiNP_033868.3. NM_009738.3.
UniGeneiMm.250719.

Genome annotation databases

EnsembliENSMUST00000029367; ENSMUSP00000029367; ENSMUSG00000027792.
GeneIDi12038.
KEGGimmu:12038.
UCSCiuc008pmv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M99492 mRNA. Translation: AAA37328.1.
AK050337 mRNA. Translation: BAC34196.1.
CH466547 Genomic DNA. Translation: EDL15482.1.
CCDSiCCDS17411.1.
PIRiS70849.
RefSeqiNP_033868.3. NM_009738.3.
UniGeneiMm.250719.

3D structure databases

ProteinModelPortaliQ03311.
SMRiQ03311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029367.

Chemistry databases

BindingDBiQ03311.
ChEMBLiCHEMBL2528.

Protein family/group databases

ESTHERimouse-BCHE. BCHE.
MEROPSiS09.980.

PTM databases

PhosphoSitePlusiQ03311.

Proteomic databases

MaxQBiQ03311.
PaxDbiQ03311.
PeptideAtlasiQ03311.
PRIDEiQ03311.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029367; ENSMUSP00000029367; ENSMUSG00000027792.
GeneIDi12038.
KEGGimmu:12038.
UCSCiuc008pmv.1. mouse.

Organism-specific databases

CTDi590.
MGIiMGI:894278. Bche.

Phylogenomic databases

eggNOGiKOG4389. Eukaryota.
COG2272. LUCA.
GeneTreeiENSGT00760000118946.
HOGENOMiHOG000091866.
HOVERGENiHBG008839.
InParanoidiQ03311.
KOiK01050.
OMAiFLGSEMW.
OrthoDBiEOG091G0I4G.
TreeFamiTF315470.

Enzyme and pathway databases

ReactomeiR-MMU-422085. Synthesis, secretion, and deacylation of Ghrelin.
SABIO-RKQ03311.

Miscellaneous databases

PROiQ03311.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027792.
CleanExiMM_BCHE.
ExpressionAtlasiQ03311. baseline and differential.
GenevisibleiQ03311. MM.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00878. CHOLNESTRASE.
ProDomiPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHLE_MOUSE
AccessioniPrimary (citable) accession number: Q03311
Secondary accession number(s): Q543J3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.