Q03308 (VPS16_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 16

Alternative name(s):
Vacuolar morphogenesis protein 9
Vacuolar protein-targeting protein 16

Gene names

Name:	VPS16
Synonyms:	VAM9, VPT16
Ordered Locus Names:	YPL045W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	798 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for vacuolar protein sorting. Required for vacuole biogenesis, stability and to maintain vacuole morphology. Required for growth at elevated temperatures. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion. Ref.5
Subunit structure	Component of the HOPS complex which is composed of PEP5, VPS16, PEP3, VPS33, VPS39 and VPS41. HOPS associates with phosphoinositides and the PX domain of VAM7. Interacts with VAM7. Ref.5
Subcellular location	Cytoplasm. Vacuole.
Miscellaneous	Present with 1300 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the VPS16 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Vacuole
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Golgi to endosome transport Inferred from genetic interaction. Source: SGD late endosome to vacuole transport Inferred from genetic interaction. Source: SGD protein targeting to vacuole Inferred from mutant phenotype. Source: SGD regulation of SNARE complex assembly Inferred from direct assay. Source: SGD regulation of vacuole fusion, non-autophagic Inferred from direct assay. Source: SGD vacuole fusion, non-autophagic Inferred from mutant phenotype. Source: SGD
Cellular component	CORVET complex Inferred from direct assay. Source: SGD HOPS complex Inferred from physical interaction. Source: SGD fungal-type vacuole membrane Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 798	798	Vacuolar protein sorting-associated protein 16		PRO_0000065891
Experimental info
	Sequence conflict	100	1	L → R in AAA35215. Ref.1
	Sequence conflict	170	1	V → I in AAA35215. Ref.1
	Sequence conflict	326 – 340	15	AIEIL…EKGVL → QLNIKEFCLREGCT in AAA35215. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q03308 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 6AE1ED43DB234137	FASTA	798	92,741
	10 20 30 40 50 60 MKNPSFDWER LKDVFYRSRA IGELKWPTQY EEFKCALSLT VIAVEIQDFI QVYNYFGQLL 70 80 90 100 110 120 GKINLQRIHE DIIKFEFDKD EKLILVTKSS IKIVKGWSPL TIESVPLQDP TIDTIWDYHN 130 140 150 160 170 180 GIMLLAKSRD IYKLNGNEWE LLYENKDKKY NLLTKNHWSC NDDSIILLDV DHVYQVSTSN 190 200 210 220 230 240 GALLKLITDS SWHKVTISSR GFICLYNMKD NKLQIFRDPA RILMEHNLDS TPDDICWCGN 250 260 270 280 290 300 DTVACSFEDE IKLYGPDGLY VTFWYPFTVT NLRAEVDGLK VITTEKIYFL SRVQPQTSNI 310 320 330 340 350 360 FRIGSTEPGA MLVDSFSLLE DHAPKAIEIL KNFVLEKGVL DCIAAAIDEF EPKLQKMLLN 370 380 390 400 410 420 AASYGKASLQ YKSFDASIFV NACNTIKLLN CFRSFGIFLT VEEYRCISLK GVIDRLLKYH 430 440 450 460 470 480 RYYECIQICK LANERFLLGY VFTEWAKDKI KGSPDMEDDE LLDKIKSRLS VIDMTDTLQM 490 500 510 520 530 540 VAVAKVAYLE GRFQLSRNLA LLEKNEEARI EQLYNLDDDS IALKECIKVQ NYSLTISLLI 550 560 570 580 590 600 ALSKKLTNSQ LTKLLIIDMF NNPLYLYYMR MDKAYLYDFY RQTDRFIDLA HVLLQQGKEQ 610 620 630 640 650 660 QSLHSFLPQI KDLYSQVQNS EVVNNTIEQL QRQEKLWIYQ ESLGKRFAIS FTNMTLDQTL 670 680 690 700 710 720 SKLIETGQDK QVKEIVKKFK ISEKKLYHLK CKTLVEAKKF DELLQFAQSR KSPIGYMPFY 730 740 750 760 770 780 TYLKSRGHMD KASPYVNMIP GLSYQEKKKL YVECRGFRDA IQLAGKEKDI PGLKEIYNII 790 PPNEPELKAL ANETMSRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The VPS16 gene product associates with a sedimentable protein complex and is essential for vacuolar protein sorting in yeast." Horazdovsky B.F., Emr S.D. J. Biol. Chem. 268:4953-4962(1993) [PubMed: 8444873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p." Stroupe C., Collins K.M., Fratti R.A., Wickner W. EMBO J. 25:1579-1589(2006) [PubMed: 16601699] [Abstract] Cited for: IDENTIFICATION IN THE HOPS COMPLEX, FUNCTION OF THE HOPS COMPLEX, INTERACTION WITH VAM7.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L07327 Genomic DNA. Translation: AAA35215.1. U44030 Genomic DNA. Translation: AAB68176.1. BK006949 Genomic DNA. Translation: DAA11385.1.
PIR	S62031.
RefSeq	NP_015280.1. NM_001183859.1.
3D structure databases
ProteinModelPortal	Q03308.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6691N.
IntAct	Q03308. 12 interactions.
MINT	MINT-663851.
STRING	Q03308.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL045W; YPL045W; YPL045W.
GeneID	856062.
KEGG	sce:YPL045W.
NMPDR	fig|4932.3.peg.6413.
Organism-specific databases
CYGD	YPL045w.
SGD	S000005966. VPS16.
Phylogenomic databases
eggNOG	fuNOG06339.
GeneTree	EFGT00050000003815.
HOGENOM	HBG203921.
OMA	CYKIRKE.
OrthoDB	EOG46DQ9W.
Gene expression databases
ArrayExpress	Q03308.
Genevestigator	Q03308.
GermOnline	YPL045W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016534. VPS16. IPR006925. Vps16_C. IPR006926. Vps16_N. [Graphical view]
Pfam	PF04840. Vps16_C. 1 hit. PF04841. Vps16_N. 1 hit. [Graphical view]
PIRSF	PIRSF007949. VPS16. 1 hit.
ProtoNet	Search...
Other
NextBio	981041.

Entry information

Entry name

VPS16_YEAST

Accession

Primary (citable) accession number: Q03308
Secondary accession number(s): D6W3W9, Q03078

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program