Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vacuolar protein sorting-associated protein 16

Gene

VPS16

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for vacuolar protein sorting. Required for vacuole biogenesis, stability and to maintain vacuole morphology. Required for growth at elevated temperatures. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion.1 Publication

GO - Biological processi

  • Golgi to endosome transport Source: SGD
  • late endosome to vacuole transport Source: SGD
  • protein targeting to vacuole Source: SGD
  • regulation of SNARE complex assembly Source: SGD
  • regulation of vacuole fusion, non-autophagic Source: SGD
  • vacuole fusion, non-autophagic Source: SGD
  • vacuole organization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33958-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 16
Alternative name(s):
Vacuolar morphogenesis protein 9
Vacuolar protein-targeting protein 16
Gene namesi
Name:VPS16
Synonyms:VAM9, VPT16
Ordered Locus Names:YPL045W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XVI

Organism-specific databases

CYGDiYPL045w.
EuPathDBiFungiDB:YPL045W.
SGDiS000005966. VPS16.

Subcellular locationi

GO - Cellular componenti

  • CORVET complex Source: SGD
  • fungal-type vacuole membrane Source: SGD
  • HOPS complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 798798Vacuolar protein sorting-associated protein 16PRO_0000065891Add
BLAST

Proteomic databases

MaxQBiQ03308.
PaxDbiQ03308.

Interactioni

Subunit structurei

Component of the HOPS complex which is composed of PEP5, VPS16, PEP3, VPS33, VPS39 and VPS41. HOPS associates with phosphoinositides and the PX domain of VAM7. Interacts with VAM7.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
VPS33P207955EBI-20355,EBI-20395

Protein-protein interaction databases

BioGridi36135. 44 interactions.
DIPiDIP-6691N.
IntActiQ03308. 13 interactions.
MINTiMINT-663851.
STRINGi4932.YPL045W.

Structurei

3D structure databases

ProteinModelPortaliQ03308.
SMRiQ03308. Positions 509-754.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the VPS16 family.Curated

Phylogenomic databases

eggNOGiNOG295765.
GeneTreeiENSGT00390000003896.
HOGENOMiHOG000141904.
InParanoidiQ03308.
OMAiCKLANER.
OrthoDBiEOG790G83.

Family and domain databases

InterProiIPR016534. VPS16.
IPR006925. Vps16_C.
IPR006926. Vps16_N.
[Graphical view]
PANTHERiPTHR12811:SF0. PTHR12811:SF0. 1 hit.
PfamiPF04840. Vps16_C. 1 hit.
PF04841. Vps16_N. 1 hit.
[Graphical view]
PIRSFiPIRSF007949. VPS16. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03308-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNPSFDWER LKDVFYRSRA IGELKWPTQY EEFKCALSLT VIAVEIQDFI
60 70 80 90 100
QVYNYFGQLL GKINLQRIHE DIIKFEFDKD EKLILVTKSS IKIVKGWSPL
110 120 130 140 150
TIESVPLQDP TIDTIWDYHN GIMLLAKSRD IYKLNGNEWE LLYENKDKKY
160 170 180 190 200
NLLTKNHWSC NDDSIILLDV DHVYQVSTSN GALLKLITDS SWHKVTISSR
210 220 230 240 250
GFICLYNMKD NKLQIFRDPA RILMEHNLDS TPDDICWCGN DTVACSFEDE
260 270 280 290 300
IKLYGPDGLY VTFWYPFTVT NLRAEVDGLK VITTEKIYFL SRVQPQTSNI
310 320 330 340 350
FRIGSTEPGA MLVDSFSLLE DHAPKAIEIL KNFVLEKGVL DCIAAAIDEF
360 370 380 390 400
EPKLQKMLLN AASYGKASLQ YKSFDASIFV NACNTIKLLN CFRSFGIFLT
410 420 430 440 450
VEEYRCISLK GVIDRLLKYH RYYECIQICK LANERFLLGY VFTEWAKDKI
460 470 480 490 500
KGSPDMEDDE LLDKIKSRLS VIDMTDTLQM VAVAKVAYLE GRFQLSRNLA
510 520 530 540 550
LLEKNEEARI EQLYNLDDDS IALKECIKVQ NYSLTISLLI ALSKKLTNSQ
560 570 580 590 600
LTKLLIIDMF NNPLYLYYMR MDKAYLYDFY RQTDRFIDLA HVLLQQGKEQ
610 620 630 640 650
QSLHSFLPQI KDLYSQVQNS EVVNNTIEQL QRQEKLWIYQ ESLGKRFAIS
660 670 680 690 700
FTNMTLDQTL SKLIETGQDK QVKEIVKKFK ISEKKLYHLK CKTLVEAKKF
710 720 730 740 750
DELLQFAQSR KSPIGYMPFY TYLKSRGHMD KASPYVNMIP GLSYQEKKKL
760 770 780 790
YVECRGFRDA IQLAGKEKDI PGLKEIYNII PPNEPELKAL ANETMSRI
Length:798
Mass (Da):92,741
Last modified:November 1, 1997 - v2
Checksum:i6AE1ED43DB234137
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti100 – 1001L → R in AAA35215 (PubMed:8444873).Curated
Sequence conflicti170 – 1701V → I in AAA35215 (PubMed:8444873).Curated
Sequence conflicti326 – 34015AIEIL…EKGVL → QLNIKEFCLREGCT in AAA35215 (PubMed:8444873).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07327 Genomic DNA. Translation: AAA35215.1.
U44030 Genomic DNA. Translation: AAB68176.1.
BK006949 Genomic DNA. Translation: DAA11385.1.
PIRiS62031.
RefSeqiNP_015280.1. NM_001183859.1.

Genome annotation databases

EnsemblFungiiYPL045W; YPL045W; YPL045W.
GeneIDi856062.
KEGGisce:YPL045W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07327 Genomic DNA. Translation: AAA35215.1.
U44030 Genomic DNA. Translation: AAB68176.1.
BK006949 Genomic DNA. Translation: DAA11385.1.
PIRiS62031.
RefSeqiNP_015280.1. NM_001183859.1.

3D structure databases

ProteinModelPortaliQ03308.
SMRiQ03308. Positions 509-754.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36135. 44 interactions.
DIPiDIP-6691N.
IntActiQ03308. 13 interactions.
MINTiMINT-663851.
STRINGi4932.YPL045W.

Proteomic databases

MaxQBiQ03308.
PaxDbiQ03308.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL045W; YPL045W; YPL045W.
GeneIDi856062.
KEGGisce:YPL045W.

Organism-specific databases

CYGDiYPL045w.
EuPathDBiFungiDB:YPL045W.
SGDiS000005966. VPS16.

Phylogenomic databases

eggNOGiNOG295765.
GeneTreeiENSGT00390000003896.
HOGENOMiHOG000141904.
InParanoidiQ03308.
OMAiCKLANER.
OrthoDBiEOG790G83.

Enzyme and pathway databases

BioCyciYEAST:G3O-33958-MONOMER.

Miscellaneous databases

NextBioi981041.
PROiQ03308.

Family and domain databases

InterProiIPR016534. VPS16.
IPR006925. Vps16_C.
IPR006926. Vps16_N.
[Graphical view]
PANTHERiPTHR12811:SF0. PTHR12811:SF0. 1 hit.
PfamiPF04840. Vps16_C. 1 hit.
PF04841. Vps16_N. 1 hit.
[Graphical view]
PIRSFiPIRSF007949. VPS16. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The VPS16 gene product associates with a sedimentable protein complex and is essential for vacuolar protein sorting in yeast."
    Horazdovsky B.F., Emr S.D.
    J. Biol. Chem. 268:4953-4962(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p."
    Stroupe C., Collins K.M., Fratti R.A., Wickner W.
    EMBO J. 25:1579-1589(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE HOPS COMPLEX, FUNCTION OF THE HOPS COMPLEX, INTERACTION WITH VAM7.

Entry informationi

Entry nameiVPS16_YEAST
AccessioniPrimary (citable) accession number: Q03308
Secondary accession number(s): D6W3W9, Q03078
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 1, 1997
Last modified: June 24, 2015
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.