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Q03308 (VPS16_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar protein sorting-associated protein 16
Alternative name(s):
Vacuolar morphogenesis protein 9
Vacuolar protein-targeting protein 16
Gene names
Name:VPS16
Synonyms:VAM9, VPT16
Ordered Locus Names:YPL045W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length798 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for vacuolar protein sorting. Required for vacuole biogenesis, stability and to maintain vacuole morphology. Required for growth at elevated temperatures. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion. Ref.5

Subunit structure

Component of the HOPS complex which is composed of PEP5, VPS16, PEP3, VPS33, VPS39 and VPS41. HOPS associates with phosphoinositides and the PX domain of VAM7. Interacts with VAM7. Ref.5

Subcellular location

Cytoplasm. Vacuole.

Miscellaneous

Present with 1300 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the VPS16 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

VPS33P207955EBI-20355,EBI-20395

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 798798Vacuolar protein sorting-associated protein 16
PRO_0000065891

Experimental info

Sequence conflict1001L → R in AAA35215. Ref.1
Sequence conflict1701V → I in AAA35215. Ref.1
Sequence conflict326 – 34015AIEIL…EKGVL → QLNIKEFCLREGCT in AAA35215. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q03308 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 6AE1ED43DB234137

FASTA79892,741
        10         20         30         40         50         60 
MKNPSFDWER LKDVFYRSRA IGELKWPTQY EEFKCALSLT VIAVEIQDFI QVYNYFGQLL 

        70         80         90        100        110        120 
GKINLQRIHE DIIKFEFDKD EKLILVTKSS IKIVKGWSPL TIESVPLQDP TIDTIWDYHN 

       130        140        150        160        170        180 
GIMLLAKSRD IYKLNGNEWE LLYENKDKKY NLLTKNHWSC NDDSIILLDV DHVYQVSTSN 

       190        200        210        220        230        240 
GALLKLITDS SWHKVTISSR GFICLYNMKD NKLQIFRDPA RILMEHNLDS TPDDICWCGN 

       250        260        270        280        290        300 
DTVACSFEDE IKLYGPDGLY VTFWYPFTVT NLRAEVDGLK VITTEKIYFL SRVQPQTSNI 

       310        320        330        340        350        360 
FRIGSTEPGA MLVDSFSLLE DHAPKAIEIL KNFVLEKGVL DCIAAAIDEF EPKLQKMLLN 

       370        380        390        400        410        420 
AASYGKASLQ YKSFDASIFV NACNTIKLLN CFRSFGIFLT VEEYRCISLK GVIDRLLKYH 

       430        440        450        460        470        480 
RYYECIQICK LANERFLLGY VFTEWAKDKI KGSPDMEDDE LLDKIKSRLS VIDMTDTLQM 

       490        500        510        520        530        540 
VAVAKVAYLE GRFQLSRNLA LLEKNEEARI EQLYNLDDDS IALKECIKVQ NYSLTISLLI 

       550        560        570        580        590        600 
ALSKKLTNSQ LTKLLIIDMF NNPLYLYYMR MDKAYLYDFY RQTDRFIDLA HVLLQQGKEQ 

       610        620        630        640        650        660 
QSLHSFLPQI KDLYSQVQNS EVVNNTIEQL QRQEKLWIYQ ESLGKRFAIS FTNMTLDQTL 

       670        680        690        700        710        720 
SKLIETGQDK QVKEIVKKFK ISEKKLYHLK CKTLVEAKKF DELLQFAQSR KSPIGYMPFY 

       730        740        750        760        770        780 
TYLKSRGHMD KASPYVNMIP GLSYQEKKKL YVECRGFRDA IQLAGKEKDI PGLKEIYNII 

       790 
PPNEPELKAL ANETMSRI 

« Hide

References

« Hide 'large scale' references
[1]"The VPS16 gene product associates with a sedimentable protein complex and is essential for vacuolar protein sorting in yeast."
Horazdovsky B.F., Emr S.D.
J. Biol. Chem. 268:4953-4962(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p."
Stroupe C., Collins K.M., Fratti R.A., Wickner W.
EMBO J. 25:1579-1589(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE HOPS COMPLEX, FUNCTION OF THE HOPS COMPLEX, INTERACTION WITH VAM7.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L07327 Genomic DNA. Translation: AAA35215.1.
U44030 Genomic DNA. Translation: AAB68176.1.
BK006949 Genomic DNA. Translation: DAA11385.1.
PIRS62031.
RefSeqNP_015280.1. NM_001183859.1.

3D structure databases

ProteinModelPortalQ03308.
SMRQ03308. Positions 509-754.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36135. 44 interactions.
DIPDIP-6691N.
IntActQ03308. 13 interactions.
MINTMINT-663851.

Proteomic databases

PaxDbQ03308.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL045W; YPL045W; YPL045W.
GeneID856062.
KEGGsce:YPL045W.

Organism-specific databases

CYGDYPL045w.
SGDS000005966. VPS16.

Phylogenomic databases

eggNOGNOG295765.
GeneTreeENSGT00390000003896.
HOGENOMHOG000141904.
OMACKLANER.
OrthoDBEOG790G83.

Enzyme and pathway databases

BioCycYEAST:G3O-33958-MONOMER.

Gene expression databases

GenevestigatorQ03308.

Family and domain databases

InterProIPR016534. VPS16.
IPR006925. Vps16_C.
IPR006926. Vps16_N.
[Graphical view]
PANTHERPTHR12811:SF0. PTHR12811:SF0. 1 hit.
PfamPF04840. Vps16_C. 1 hit.
PF04841. Vps16_N. 1 hit.
[Graphical view]
PIRSFPIRSF007949. VPS16. 1 hit.
ProtoNetSearch...

Other

NextBio981041.
PROQ03308.

Entry information

Entry nameVPS16_YEAST
AccessionPrimary (citable) accession number: Q03308
Secondary accession number(s): D6W3W9, Q03078
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families