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Q03308

- VPS16_YEAST

UniProt

Q03308 - VPS16_YEAST

Protein

Vacuolar protein sorting-associated protein 16

Gene

VPS16

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Essential for vacuolar protein sorting. Required for vacuole biogenesis, stability and to maintain vacuole morphology. Required for growth at elevated temperatures. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. Golgi to endosome transport Source: SGD
    2. late endosome to vacuole transport Source: SGD
    3. protein targeting to vacuole Source: SGD
    4. regulation of SNARE complex assembly Source: SGD
    5. regulation of vacuole fusion, non-autophagic Source: SGD
    6. vacuole fusion, non-autophagic Source: SGD
    7. vacuole organization Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33958-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar protein sorting-associated protein 16
    Alternative name(s):
    Vacuolar morphogenesis protein 9
    Vacuolar protein-targeting protein 16
    Gene namesi
    Name:VPS16
    Synonyms:VAM9, VPT16
    Ordered Locus Names:YPL045W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL045w.
    SGDiS000005966. VPS16.

    Subcellular locationi

    GO - Cellular componenti

    1. CORVET complex Source: SGD
    2. fungal-type vacuole membrane Source: SGD
    3. HOPS complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 798798Vacuolar protein sorting-associated protein 16PRO_0000065891Add
    BLAST

    Proteomic databases

    MaxQBiQ03308.
    PaxDbiQ03308.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03308.

    Interactioni

    Subunit structurei

    Component of the HOPS complex which is composed of PEP5, VPS16, PEP3, VPS33, VPS39 and VPS41. HOPS associates with phosphoinositides and the PX domain of VAM7. Interacts with VAM7.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    VPS33P207955EBI-20355,EBI-20395

    Protein-protein interaction databases

    BioGridi36135. 44 interactions.
    DIPiDIP-6691N.
    IntActiQ03308. 13 interactions.
    MINTiMINT-663851.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03308.
    SMRiQ03308. Positions 509-754.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the VPS16 family.Curated

    Phylogenomic databases

    eggNOGiNOG295765.
    GeneTreeiENSGT00390000003896.
    HOGENOMiHOG000141904.
    OMAiCKLANER.
    OrthoDBiEOG790G83.

    Family and domain databases

    InterProiIPR016534. VPS16.
    IPR006925. Vps16_C.
    IPR006926. Vps16_N.
    [Graphical view]
    PANTHERiPTHR12811:SF0. PTHR12811:SF0. 1 hit.
    PfamiPF04840. Vps16_C. 1 hit.
    PF04841. Vps16_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF007949. VPS16. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q03308-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKNPSFDWER LKDVFYRSRA IGELKWPTQY EEFKCALSLT VIAVEIQDFI    50
    QVYNYFGQLL GKINLQRIHE DIIKFEFDKD EKLILVTKSS IKIVKGWSPL 100
    TIESVPLQDP TIDTIWDYHN GIMLLAKSRD IYKLNGNEWE LLYENKDKKY 150
    NLLTKNHWSC NDDSIILLDV DHVYQVSTSN GALLKLITDS SWHKVTISSR 200
    GFICLYNMKD NKLQIFRDPA RILMEHNLDS TPDDICWCGN DTVACSFEDE 250
    IKLYGPDGLY VTFWYPFTVT NLRAEVDGLK VITTEKIYFL SRVQPQTSNI 300
    FRIGSTEPGA MLVDSFSLLE DHAPKAIEIL KNFVLEKGVL DCIAAAIDEF 350
    EPKLQKMLLN AASYGKASLQ YKSFDASIFV NACNTIKLLN CFRSFGIFLT 400
    VEEYRCISLK GVIDRLLKYH RYYECIQICK LANERFLLGY VFTEWAKDKI 450
    KGSPDMEDDE LLDKIKSRLS VIDMTDTLQM VAVAKVAYLE GRFQLSRNLA 500
    LLEKNEEARI EQLYNLDDDS IALKECIKVQ NYSLTISLLI ALSKKLTNSQ 550
    LTKLLIIDMF NNPLYLYYMR MDKAYLYDFY RQTDRFIDLA HVLLQQGKEQ 600
    QSLHSFLPQI KDLYSQVQNS EVVNNTIEQL QRQEKLWIYQ ESLGKRFAIS 650
    FTNMTLDQTL SKLIETGQDK QVKEIVKKFK ISEKKLYHLK CKTLVEAKKF 700
    DELLQFAQSR KSPIGYMPFY TYLKSRGHMD KASPYVNMIP GLSYQEKKKL 750
    YVECRGFRDA IQLAGKEKDI PGLKEIYNII PPNEPELKAL ANETMSRI 798
    Length:798
    Mass (Da):92,741
    Last modified:November 1, 1997 - v2
    Checksum:i6AE1ED43DB234137
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti100 – 1001L → R in AAA35215. (PubMed:8444873)Curated
    Sequence conflicti170 – 1701V → I in AAA35215. (PubMed:8444873)Curated
    Sequence conflicti326 – 34015AIEIL…EKGVL → QLNIKEFCLREGCT in AAA35215. (PubMed:8444873)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07327 Genomic DNA. Translation: AAA35215.1.
    U44030 Genomic DNA. Translation: AAB68176.1.
    BK006949 Genomic DNA. Translation: DAA11385.1.
    PIRiS62031.
    RefSeqiNP_015280.1. NM_001183859.1.

    Genome annotation databases

    EnsemblFungiiYPL045W; YPL045W; YPL045W.
    GeneIDi856062.
    KEGGisce:YPL045W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07327 Genomic DNA. Translation: AAA35215.1 .
    U44030 Genomic DNA. Translation: AAB68176.1 .
    BK006949 Genomic DNA. Translation: DAA11385.1 .
    PIRi S62031.
    RefSeqi NP_015280.1. NM_001183859.1.

    3D structure databases

    ProteinModelPortali Q03308.
    SMRi Q03308. Positions 509-754.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36135. 44 interactions.
    DIPi DIP-6691N.
    IntActi Q03308. 13 interactions.
    MINTi MINT-663851.

    Proteomic databases

    MaxQBi Q03308.
    PaxDbi Q03308.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL045W ; YPL045W ; YPL045W .
    GeneIDi 856062.
    KEGGi sce:YPL045W.

    Organism-specific databases

    CYGDi YPL045w.
    SGDi S000005966. VPS16.

    Phylogenomic databases

    eggNOGi NOG295765.
    GeneTreei ENSGT00390000003896.
    HOGENOMi HOG000141904.
    OMAi CKLANER.
    OrthoDBi EOG790G83.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33958-MONOMER.

    Miscellaneous databases

    NextBioi 981041.
    PROi Q03308.

    Gene expression databases

    Genevestigatori Q03308.

    Family and domain databases

    InterProi IPR016534. VPS16.
    IPR006925. Vps16_C.
    IPR006926. Vps16_N.
    [Graphical view ]
    PANTHERi PTHR12811:SF0. PTHR12811:SF0. 1 hit.
    Pfami PF04840. Vps16_C. 1 hit.
    PF04841. Vps16_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF007949. VPS16. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The VPS16 gene product associates with a sedimentable protein complex and is essential for vacuolar protein sorting in yeast."
      Horazdovsky B.F., Emr S.D.
      J. Biol. Chem. 268:4953-4962(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p."
      Stroupe C., Collins K.M., Fratti R.A., Wickner W.
      EMBO J. 25:1579-1589(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE HOPS COMPLEX, FUNCTION OF THE HOPS COMPLEX, INTERACTION WITH VAM7.

    Entry informationi

    Entry nameiVPS16_YEAST
    AccessioniPrimary (citable) accession number: Q03308
    Secondary accession number(s): D6W3W9, Q03078
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3