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Protein

Vacuolar protein sorting-associated protein 16

Gene

VPS16

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential for vacuolar protein sorting. Required for vacuole biogenesis, stability and to maintain vacuole morphology. Required for growth at elevated temperatures. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion.1 Publication

Miscellaneous

Present with 1300 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • Golgi to endosome transport Source: SGD
  • late endosome to vacuole transport Source: SGD
  • protein targeting to vacuole Source: SGD
  • regulation of SNARE complex assembly Source: SGD
  • regulation of vacuole fusion, non-autophagic Source: SGD
  • vacuole fusion, non-autophagic Source: SGD
  • vacuole organization Source: SGD
  • vesicle tethering Source: SGD

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33958-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 16
Alternative name(s):
Vacuolar morphogenesis protein 9
Vacuolar protein-targeting protein 16
Gene namesi
Name:VPS16
Synonyms:VAM9, VPT16
Ordered Locus Names:YPL045W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL045W
SGDiS000005966 VPS16

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000658911 – 798Vacuolar protein sorting-associated protein 16Add BLAST798

Proteomic databases

MaxQBiQ03308
PaxDbiQ03308
PRIDEiQ03308

Interactioni

Subunit structurei

Component of the HOPS complex which is composed of PEP5, VPS16, PEP3, VPS33, VPS39 and VPS41. HOPS associates with phosphoinositides and the PX domain of VAM7. Interacts with VAM7.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
VPS33P207955EBI-20355,EBI-20395

GO - Molecular functioni

Protein-protein interaction databases

BioGridi36135, 48 interactors
DIPiDIP-6691N
IntActiQ03308, 13 interactors
MINTiQ03308
STRINGi4932.YPL045W

Structurei

3D structure databases

ProteinModelPortaliQ03308
SMRiQ03308
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the VPS16 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000003896
HOGENOMiHOG000141904
InParanoidiQ03308
KOiK20180
OMAiSYCLLGR
OrthoDBiEOG092C0FSR

Family and domain databases

Gene3Di1.10.150.780, 1 hit
InterProiView protein in InterPro
IPR016534 VPS16
IPR006925 Vps16_C
IPR038132 Vps16_C_sf
IPR006926 Vps16_N
PANTHERiPTHR12811 PTHR12811, 1 hit
PfamiView protein in Pfam
PF04840 Vps16_C, 1 hit
PF04841 Vps16_N, 1 hit
PIRSFiPIRSF007949 VPS16, 1 hit

Sequencei

Sequence statusi: Complete.

Q03308-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNPSFDWER LKDVFYRSRA IGELKWPTQY EEFKCALSLT VIAVEIQDFI
60 70 80 90 100
QVYNYFGQLL GKINLQRIHE DIIKFEFDKD EKLILVTKSS IKIVKGWSPL
110 120 130 140 150
TIESVPLQDP TIDTIWDYHN GIMLLAKSRD IYKLNGNEWE LLYENKDKKY
160 170 180 190 200
NLLTKNHWSC NDDSIILLDV DHVYQVSTSN GALLKLITDS SWHKVTISSR
210 220 230 240 250
GFICLYNMKD NKLQIFRDPA RILMEHNLDS TPDDICWCGN DTVACSFEDE
260 270 280 290 300
IKLYGPDGLY VTFWYPFTVT NLRAEVDGLK VITTEKIYFL SRVQPQTSNI
310 320 330 340 350
FRIGSTEPGA MLVDSFSLLE DHAPKAIEIL KNFVLEKGVL DCIAAAIDEF
360 370 380 390 400
EPKLQKMLLN AASYGKASLQ YKSFDASIFV NACNTIKLLN CFRSFGIFLT
410 420 430 440 450
VEEYRCISLK GVIDRLLKYH RYYECIQICK LANERFLLGY VFTEWAKDKI
460 470 480 490 500
KGSPDMEDDE LLDKIKSRLS VIDMTDTLQM VAVAKVAYLE GRFQLSRNLA
510 520 530 540 550
LLEKNEEARI EQLYNLDDDS IALKECIKVQ NYSLTISLLI ALSKKLTNSQ
560 570 580 590 600
LTKLLIIDMF NNPLYLYYMR MDKAYLYDFY RQTDRFIDLA HVLLQQGKEQ
610 620 630 640 650
QSLHSFLPQI KDLYSQVQNS EVVNNTIEQL QRQEKLWIYQ ESLGKRFAIS
660 670 680 690 700
FTNMTLDQTL SKLIETGQDK QVKEIVKKFK ISEKKLYHLK CKTLVEAKKF
710 720 730 740 750
DELLQFAQSR KSPIGYMPFY TYLKSRGHMD KASPYVNMIP GLSYQEKKKL
760 770 780 790
YVECRGFRDA IQLAGKEKDI PGLKEIYNII PPNEPELKAL ANETMSRI
Length:798
Mass (Da):92,741
Last modified:November 1, 1997 - v2
Checksum:i6AE1ED43DB234137
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti100L → R in AAA35215 (PubMed:8444873).Curated1
Sequence conflicti170V → I in AAA35215 (PubMed:8444873).Curated1
Sequence conflicti326 – 340AIEIL…EKGVL → QLNIKEFCLREGCT in AAA35215 (PubMed:8444873).CuratedAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07327 Genomic DNA Translation: AAA35215.1
U44030 Genomic DNA Translation: AAB68176.1
BK006949 Genomic DNA Translation: DAA11385.1
PIRiS62031
RefSeqiNP_015280.1, NM_001183859.1

Genome annotation databases

EnsemblFungiiYPL045W; YPL045W; YPL045W
GeneIDi856062
KEGGisce:YPL045W

Similar proteinsi

Entry informationi

Entry nameiVPS16_YEAST
AccessioniPrimary (citable) accession number: Q03308
Secondary accession number(s): D6W3W9, Q03078
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
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