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Q03308

- VPS16_YEAST

UniProt

Q03308 - VPS16_YEAST

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Protein

Vacuolar protein sorting-associated protein 16

Gene
VPS16, VAM9, VPT16, YPL045W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential for vacuolar protein sorting. Required for vacuole biogenesis, stability and to maintain vacuole morphology. Required for growth at elevated temperatures. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion.1 Publication

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. Golgi to endosome transport Source: SGD
  2. late endosome to vacuole transport Source: SGD
  3. protein targeting to vacuole Source: SGD
  4. regulation of SNARE complex assembly Source: SGD
  5. regulation of vacuole fusion, non-autophagic Source: SGD
  6. vacuole fusion, non-autophagic Source: SGD
  7. vacuole organization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33958-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 16
Alternative name(s):
Vacuolar morphogenesis protein 9
Vacuolar protein-targeting protein 16
Gene namesi
Name:VPS16
Synonyms:VAM9, VPT16
Ordered Locus Names:YPL045W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL045w.
SGDiS000005966. VPS16.

Subcellular locationi

GO - Cellular componenti

  1. CORVET complex Source: SGD
  2. fungal-type vacuole membrane Source: SGD
  3. HOPS complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 798798Vacuolar protein sorting-associated protein 16PRO_0000065891Add
BLAST

Proteomic databases

MaxQBiQ03308.
PaxDbiQ03308.

Expressioni

Gene expression databases

GenevestigatoriQ03308.

Interactioni

Subunit structurei

Component of the HOPS complex which is composed of PEP5, VPS16, PEP3, VPS33, VPS39 and VPS41. HOPS associates with phosphoinositides and the PX domain of VAM7. Interacts with VAM7.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
VPS33P207955EBI-20355,EBI-20395

Protein-protein interaction databases

BioGridi36135. 44 interactions.
DIPiDIP-6691N.
IntActiQ03308. 13 interactions.
MINTiMINT-663851.

Structurei

3D structure databases

ProteinModelPortaliQ03308.
SMRiQ03308. Positions 509-754.

Family & Domainsi

Sequence similaritiesi

Belongs to the VPS16 family.

Phylogenomic databases

eggNOGiNOG295765.
GeneTreeiENSGT00390000003896.
HOGENOMiHOG000141904.
OMAiCKLANER.
OrthoDBiEOG790G83.

Family and domain databases

InterProiIPR016534. VPS16.
IPR006925. Vps16_C.
IPR006926. Vps16_N.
[Graphical view]
PANTHERiPTHR12811:SF0. PTHR12811:SF0. 1 hit.
PfamiPF04840. Vps16_C. 1 hit.
PF04841. Vps16_N. 1 hit.
[Graphical view]
PIRSFiPIRSF007949. VPS16. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03308-1 [UniParc]FASTAAdd to Basket

« Hide

MKNPSFDWER LKDVFYRSRA IGELKWPTQY EEFKCALSLT VIAVEIQDFI    50
QVYNYFGQLL GKINLQRIHE DIIKFEFDKD EKLILVTKSS IKIVKGWSPL 100
TIESVPLQDP TIDTIWDYHN GIMLLAKSRD IYKLNGNEWE LLYENKDKKY 150
NLLTKNHWSC NDDSIILLDV DHVYQVSTSN GALLKLITDS SWHKVTISSR 200
GFICLYNMKD NKLQIFRDPA RILMEHNLDS TPDDICWCGN DTVACSFEDE 250
IKLYGPDGLY VTFWYPFTVT NLRAEVDGLK VITTEKIYFL SRVQPQTSNI 300
FRIGSTEPGA MLVDSFSLLE DHAPKAIEIL KNFVLEKGVL DCIAAAIDEF 350
EPKLQKMLLN AASYGKASLQ YKSFDASIFV NACNTIKLLN CFRSFGIFLT 400
VEEYRCISLK GVIDRLLKYH RYYECIQICK LANERFLLGY VFTEWAKDKI 450
KGSPDMEDDE LLDKIKSRLS VIDMTDTLQM VAVAKVAYLE GRFQLSRNLA 500
LLEKNEEARI EQLYNLDDDS IALKECIKVQ NYSLTISLLI ALSKKLTNSQ 550
LTKLLIIDMF NNPLYLYYMR MDKAYLYDFY RQTDRFIDLA HVLLQQGKEQ 600
QSLHSFLPQI KDLYSQVQNS EVVNNTIEQL QRQEKLWIYQ ESLGKRFAIS 650
FTNMTLDQTL SKLIETGQDK QVKEIVKKFK ISEKKLYHLK CKTLVEAKKF 700
DELLQFAQSR KSPIGYMPFY TYLKSRGHMD KASPYVNMIP GLSYQEKKKL 750
YVECRGFRDA IQLAGKEKDI PGLKEIYNII PPNEPELKAL ANETMSRI 798
Length:798
Mass (Da):92,741
Last modified:November 1, 1997 - v2
Checksum:i6AE1ED43DB234137
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti100 – 1001L → R in AAA35215. 1 Publication
Sequence conflicti170 – 1701V → I in AAA35215. 1 Publication
Sequence conflicti326 – 34015AIEIL…EKGVL → QLNIKEFCLREGCT in AAA35215. 1 PublicationAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07327 Genomic DNA. Translation: AAA35215.1.
U44030 Genomic DNA. Translation: AAB68176.1.
BK006949 Genomic DNA. Translation: DAA11385.1.
PIRiS62031.
RefSeqiNP_015280.1. NM_001183859.1.

Genome annotation databases

EnsemblFungiiYPL045W; YPL045W; YPL045W.
GeneIDi856062.
KEGGisce:YPL045W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07327 Genomic DNA. Translation: AAA35215.1 .
U44030 Genomic DNA. Translation: AAB68176.1 .
BK006949 Genomic DNA. Translation: DAA11385.1 .
PIRi S62031.
RefSeqi NP_015280.1. NM_001183859.1.

3D structure databases

ProteinModelPortali Q03308.
SMRi Q03308. Positions 509-754.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36135. 44 interactions.
DIPi DIP-6691N.
IntActi Q03308. 13 interactions.
MINTi MINT-663851.

Proteomic databases

MaxQBi Q03308.
PaxDbi Q03308.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL045W ; YPL045W ; YPL045W .
GeneIDi 856062.
KEGGi sce:YPL045W.

Organism-specific databases

CYGDi YPL045w.
SGDi S000005966. VPS16.

Phylogenomic databases

eggNOGi NOG295765.
GeneTreei ENSGT00390000003896.
HOGENOMi HOG000141904.
OMAi CKLANER.
OrthoDBi EOG790G83.

Enzyme and pathway databases

BioCyci YEAST:G3O-33958-MONOMER.

Miscellaneous databases

NextBioi 981041.
PROi Q03308.

Gene expression databases

Genevestigatori Q03308.

Family and domain databases

InterProi IPR016534. VPS16.
IPR006925. Vps16_C.
IPR006926. Vps16_N.
[Graphical view ]
PANTHERi PTHR12811:SF0. PTHR12811:SF0. 1 hit.
Pfami PF04840. Vps16_C. 1 hit.
PF04841. Vps16_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF007949. VPS16. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The VPS16 gene product associates with a sedimentable protein complex and is essential for vacuolar protein sorting in yeast."
    Horazdovsky B.F., Emr S.D.
    J. Biol. Chem. 268:4953-4962(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p."
    Stroupe C., Collins K.M., Fratti R.A., Wickner W.
    EMBO J. 25:1579-1589(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE HOPS COMPLEX, FUNCTION OF THE HOPS COMPLEX, INTERACTION WITH VAM7.

Entry informationi

Entry nameiVPS16_YEAST
AccessioniPrimary (citable) accession number: Q03308
Secondary accession number(s): D6W3W9, Q03078
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1300 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi