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Protein

Serine/threonine-protein kinase PKH3

Gene

PKH3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase which may phosphorylate the same targets substrates as PKH1 and PKH2, 2 upstream activators of PKC1.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATPPROSITE-ProRule annotation
Active sitei138 – 1381Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 259ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: SGD
  • protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • MAPK cascade involved in cell wall organization or biogenesis Source: SGD
  • peptidyl-serine phosphorylation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29994-MONOMER.
ReactomeiR-SCE-114604. GPVI-mediated activation cascade.
R-SCE-1257604. PIP3 activates AKT signaling.
R-SCE-165158. Activation of AKT2.
R-SCE-202424. Downstream TCR signaling.
R-SCE-2730905. Role of LAT2/NTAL/LAB on calcium mobilization.
R-SCE-389357. CD28 dependent PI3K/Akt signaling.
R-SCE-389513. CTLA4 inhibitory signaling.
R-SCE-392451. G beta:gamma signalling through PI3Kgamma.
R-SCE-444257. RSK activation.
R-SCE-5218920. VEGFR2 mediated vascular permeability.
R-SCE-5218921. VEGFR2 mediated cell proliferation.
R-SCE-5625740. RHO GTPases activate PKNs.
R-SCE-6804757. Regulation of TP53 Degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PKH3 (EC:2.7.11.1)
Alternative name(s):
Pkb-activating kinase homolog 3
Gene namesi
Name:PKH3
Ordered Locus Names:YDR466W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR466W.
SGDiS000002874. PKH3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 898898Serine/threonine-protein kinase PKH3PRO_0000086554Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei696 – 6961PhosphoserineCombined sources
Modified residuei753 – 7531PhosphoserineCombined sources
Modified residuei871 – 8711PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03306.

PTM databases

iPTMnetiQ03306.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MYO5Q044392EBI-37683,EBI-11687
PAM1P373043EBI-37683,EBI-12870

Protein-protein interaction databases

BioGridi32520. 68 interactions.
DIPiDIP-8781N.
IntActiQ03306. 16 interactions.
MINTiMINT-1558381.

Structurei

3D structure databases

ProteinModelPortaliQ03306.
SMRiQ03306. Positions 5-321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 293283Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074819.
HOGENOMiHOG000001069.
InParanoidiQ03306.
KOiK06276.
OMAiQMANIED.
OrthoDBiEOG092C0HH3.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03306-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSRKRSPHD FIFKEELGHG SYSTVFKALD KKSPNKIYAI KVCSKKHIIK
60 70 80 90 100
EAKVKYVTIE KNTMNLLAQK HHAGIIKLYY TFHDEENLYF VLDFAPGGEL
110 120 130 140 150
LSLLHKMGTF NDIWTRHFTA QLIDALEFIH SHGIIHRDLK PENVLLDRDG
160 170 180 190 200
RLMITDFGAA ATIDPSLSGD SAKFNSDSNG SKDNQNCASF VGTAEYVSPE
210 220 230 240 250
LLLYNQCGYG SDIWALGCMI YQFVQGQPPF RGENELKTFE KIVALDYPWG
260 270 280 290 300
PNNRINNSTS PINPLVINLV QKILVIEVNE RISLEQIKRH PYFSKVDWND
310 320 330 340 350
KIKIWRGIWQ SQGQSLQQTT LGLPNIPQNI LPTRQLHVID TPARSIQITK
360 370 380 390 400
QKRKKPTKIS NTTSSIVVWR KRLGISTGKD DLGTVPSTTP AVTAPNDTNV
410 420 430 440 450
LTNTAAHSTA NIALPPNSQS NQVKRAQLVA PNRIPPKVPV INDNVRNKSI
460 470 480 490 500
PRTKPNVPPL QTSSIPQKLS TSSASSALSA PSTEIRNQDL THTLDGRNSI
510 520 530 540 550
DIHVLKQDYV FIYGIPYEHE GPAMSLNSYN KIDNDLITSL VAQHKEELKN
560 570 580 590 600
SESFLQVLTL KKSGMLSYKN TVMEGNDDQE NKEHQMANIE DTDLSMYDFE
610 620 630 640 650
FNELTRKGFL ILEKYKNRIW FISLPSYSTL SKIPFNAVKS STINNNENWV
660 670 680 690 700
DCFFRARQLL EEKQILDKIS NVSFDSKASS EPSSPPPISR KERPLSIGNN
710 720 730 740 750
VTTLSYTAKN GSQNNAPQND NVGEEKPFRI PSSTKDRPGA NSTPSSRHPR
760 770 780 790 800
VLSSNNAGET PKKMNGRLPN SAPSTNTYTN GSVPAFNHRP STNVGNNKHN
810 820 830 840 850
ILTSKKQGSS VFSPSSSTTK PQIKTTGYRQ PTPSPPLPQM EFPTTREKYS
860 870 880 890
APSNMVISSS RYEVLHTLNN SQTNFDREIA SRGASAAFRS LQKSKKKK
Length:898
Mass (Da):100,476
Last modified:November 1, 1996 - v1
Checksum:i44715C40DD26B89E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA. Translation: AAB64902.1.
BK006938 Genomic DNA. Translation: DAA12301.1.
PIRiS69634.
RefSeqiNP_010754.1. NM_001180774.1.

Genome annotation databases

EnsemblFungiiYDR466W; YDR466W; YDR466W.
GeneIDi852077.
KEGGisce:YDR466W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33050 Genomic DNA. Translation: AAB64902.1.
BK006938 Genomic DNA. Translation: DAA12301.1.
PIRiS69634.
RefSeqiNP_010754.1. NM_001180774.1.

3D structure databases

ProteinModelPortaliQ03306.
SMRiQ03306. Positions 5-321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32520. 68 interactions.
DIPiDIP-8781N.
IntActiQ03306. 16 interactions.
MINTiMINT-1558381.

PTM databases

iPTMnetiQ03306.

Proteomic databases

MaxQBiQ03306.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR466W; YDR466W; YDR466W.
GeneIDi852077.
KEGGisce:YDR466W.

Organism-specific databases

EuPathDBiFungiDB:YDR466W.
SGDiS000002874. PKH3.

Phylogenomic databases

GeneTreeiENSGT00550000074819.
HOGENOMiHOG000001069.
InParanoidiQ03306.
KOiK06276.
OMAiQMANIED.
OrthoDBiEOG092C0HH3.

Enzyme and pathway databases

BioCyciYEAST:G3O-29994-MONOMER.
ReactomeiR-SCE-114604. GPVI-mediated activation cascade.
R-SCE-1257604. PIP3 activates AKT signaling.
R-SCE-165158. Activation of AKT2.
R-SCE-202424. Downstream TCR signaling.
R-SCE-2730905. Role of LAT2/NTAL/LAB on calcium mobilization.
R-SCE-389357. CD28 dependent PI3K/Akt signaling.
R-SCE-389513. CTLA4 inhibitory signaling.
R-SCE-392451. G beta:gamma signalling through PI3Kgamma.
R-SCE-444257. RSK activation.
R-SCE-5218920. VEGFR2 mediated vascular permeability.
R-SCE-5218921. VEGFR2 mediated cell proliferation.
R-SCE-5625740. RHO GTPases activate PKNs.
R-SCE-6804757. Regulation of TP53 Degradation.

Miscellaneous databases

PROiQ03306.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPKH3_YEAST
AccessioniPrimary (citable) accession number: Q03306
Secondary accession number(s): D6VT91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.