Q03304 (TMOF_PSEME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Toluene-4-monooxygenase electron transfer component

Including the following 2 domains:

1. Ferredoxin
2. Ferredoxin--NAD(+) reductase
   EC=1.18.1.3

Gene names

Name:

tmoF

Organism

Pseudomonas mendocina

Taxonomic identifier

300 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Electron transfer component of toluene 4-monooxygenase complex.
Catalytic activity	Reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH.
Cofactor	FAD. Binds 1 2Fe-2S cluster per subunit By similarity.
Pathway	Xenobiotic degradation; toluene degradation.
Subunit structure	The multicomponent enzyme toluene-4-monooxygenase is formed by the tmoA, tmoB, tmoC, tmoD, tmoE and tmoF polypeptides.
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin reductase family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	2Fe-2S FAD Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro ferredoxin-NAD+ reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Toluene-4-monooxygenase electron transfer component		PRO_0000167658
Regions
	Domain	1 – 92	92	2Fe-2S ferredoxin-type
	Domain	100 – 195	96	FAD-binding FR-type
	Region	95 – 326	232	Ferredoxin-reductase
Sites
	Metal binding	36	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	41	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	44	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	76	1	Iron-sulfur (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q03304 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 17889D794FC092EE	FASTA	326	35,983
	10 20 30 40 50 60 MFNIQSDDLL HHFEADSNDT LLSAALRAEL VFPYECNSGG CGACKIELLE GEVSNLWPDA 70 80 90 100 110 120 PGLAARELRK NRFLACQCKP LSDLKIKVIN RAEGRASHPP KRFSTRVVSK RFLSDEMFEL 130 140 150 160 170 180 RLEAEQKVVF SPGQYFMVDV PELGTRAYSA ANPVDGNTLT LIVKAVPNGK VSCALANETI 190 200 210 220 230 240 ETLQLDGPYG LSVLKTADET QSVFIAGGSG IAPMVSMVNT LIAQGYEKPI TVFYGSRLEA 250 260 270 280 290 300 ELEAAETLFG WKENLKLINV SSSVVGNSEK KYPTGYVHEI IPEYMEGLLG AEFYLCGPPQ 310 320 MINSVQKLLM IENKVPFEAI HFDRFF

References

[1]	"Identification of a new gene, tmoF, in the Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase." Yen K.-M., Karl M.R. J. Bacteriol. 174:7253-7261(1992) [PubMed: 1429451] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-14. Strain: KR1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M95045 Genomic DNA. Translation: AAA26004.1.
PIR	A47016.
3D structure databases
ProteinModelPortal	Q03304.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR012675. Beta-grasp_ferredoxin-type. IPR017927. Fd_Rdtase_FAD-bd. IPR001041. Ferredoxin. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR001221. Phe_hydroxylase. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00111. Fer2. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00371. FPNCR. PR00410. PHEHYDRXLASE.
SUPFAM	SSF54292. Ferredoxin. 1 hit. SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TMOF_PSEME

Accession

Primary (citable) accession number: Q03304

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program