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Protein

RNA polymerase II transcription factor B subunit 3

Gene

TFB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the general transcription and DNA repair factor IIH (TFIIH or factor B), which is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. TFIIH has CTD kinase and DNA-dependent ATPase activity, and is essential for polymerase II transcription in vitro.2 Publications

Miscellaneous

Present with 3050 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri13 – 60RING-typePROSITE-ProRule annotationAdd BLAST48

GO - Molecular functioni

GO - Biological processi

  • nucleotide-excision repair Source: SGD
  • phosphorylation of RNA polymerase II C-terminal domain Source: SGD
  • regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
  • transcription by RNA polymerase II Source: SGD

Keywordsi

Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29988-MONOMER
ReactomeiR-SCE-113418 Formation of the Early Elongation Complex
R-SCE-5696395 Formation of Incision Complex in GG-NER
R-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-6781823 Formation of TC-NER Pre-Incision Complex
R-SCE-6782135 Dual incision in TC-NER
R-SCE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SCE-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-SCE-69202 Cyclin E associated events during G1/S transition
R-SCE-69273 Cyclin A/B1/B2 associated events during G2/M transition
R-SCE-69656 Cyclin A:Cdk2-associated events at S phase entry
R-SCE-72086 mRNA Capping
R-SCE-73776 RNA Polymerase II Promoter Escape
R-SCE-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953 RNA Polymerase II Transcription Initiation
R-SCE-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-SCE-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II transcription factor B subunit 3
Alternative name(s):
RNA polymerase II transcription factor B 38 kDa subunit
RNA polymerase II transcription factor B p38 subunit
Gene namesi
Name:TFB3
Synonyms:RIG2
Ordered Locus Names:YDR460W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR460W
SGDiS000002868 TFB3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000559421 – 321RNA polymerase II transcription factor B subunit 3Add BLAST321

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei157PhosphoserineCombined sources1
Modified residuei258PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03290
PaxDbiQ03290
PRIDEiQ03290

PTM databases

iPTMnetiQ03290

Interactioni

Subunit structurei

Component of the transcription factor IIH (TFIIH) holo but not the TFIIH core complex. Component of a complex consisting of KIN28, CCL1 and TFB3; the KIN28-CCL1 dimer is known as the TFIIK complex.4 Publications

Protein-protein interaction databases

BioGridi32514, 35 interactors
ComplexPortaliCPX-1659 General transcription factor complex TFIIH
CPX-1660 General transcription factor complex TFIIK
DIPiDIP-2398N
IntActiQ03290, 16 interactors
MINTiQ03290
STRINGi4932.YDR460W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5OQJelectron microscopy4.7031-321[»]
5OQMelectron microscopy5.8031-321[»]
ProteinModelPortaliQ03290
SMRiQ03290
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri13 – 60RING-typePROSITE-ProRule annotationAdd BLAST48

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000002319
HOGENOMiHOG000189680
InParanoidiQ03290
KOiK10842
OMAiPYDPFGG
OrthoDBiEOG092C4WQO

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR015877 Cdk-activating_kinase_MAT1_cen
IPR004575 MAT1/Tfb3
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR12683:SF13 PTHR12683:SF13, 1 hit
PfamiView protein in Pfam
PF06391 MAT1, 1 hit
PF17121 zf-C3HC4_5, 1 hit
PIRSFiPIRSF003338 MAT1_metazoa, 1 hit
TIGRFAMsiTIGR00570 cdk7, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q03290-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLMDEYEENK DMCPICKTDR YLSPDVKFLV NPECYHRICE SCVDRIFSLG
60 70 80 90 100
PAQCPYKGCD KILRKNKFKT QIFDDVEVEK EVDIRKRVFN VFNKTIDDFN
110 120 130 140 150
GDLVEYNKYL EEVEDIIYKL DHGIDVAKTE EKLRTYEELN KQLIMNNLER
160 170 180 190 200
SRTEIESFEQ RQKFEKEMKL KKRLLERQIE EEERMNKEWT KKEIVNRLST
210 220 230 240 250
TTQDINETIE GVKNTVKLKK SSARRKLEEL NRVLKNNPYF NSNVNVQNSR
260 270 280 290 300
LKDAVPFTPF NGDREAHPRF TLKGSVYNDP FIKDLEHRKE FIASGFNTNY
310 320
AYERVLTEAF MGLGCVISEE L
Length:321
Mass (Da):38,128
Last modified:November 1, 1996 - v1
Checksum:i528D61CF25ADFAF4
GO

Sequence cautioni

The sequence AAB40629 differs from that shown. Reason: Frameshift at position 262.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62805 Genomic DNA Translation: AAB40629.1 Frameshift.
U33050 Genomic DNA Translation: AAB64899.1
BK006938 Genomic DNA Translation: DAA12294.1
PIRiS69628
RefSeqiNP_010748.3, NM_001180768.3

Genome annotation databases

EnsemblFungiiYDR460W; YDR460W; YDR460W
GeneIDi852071
KEGGisce:YDR460W

Similar proteinsi

Entry informationi

Entry nameiTFB3_YEAST
AccessioniPrimary (citable) accession number: Q03290
Secondary accession number(s): D6VT84, P89104
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: November 1, 1996
Last modified: June 20, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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