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Q03290

- TFB3_YEAST

UniProt

Q03290 - TFB3_YEAST

Protein

RNA polymerase II transcription factor B subunit 3

Gene

TFB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Acts as component of the general transcription and DNA repair factor IIH (TFIIH or factor B), which is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. TFIIH has CTD kinase and DNA-dependent ATPase activity, and is essential for polymerase II transcription in vitro.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri13 – 6048RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cell cycle Source: InterPro
    2. nucleotide-excision repair Source: SGD
    3. phosphorylation of RNA polymerase II C-terminal domain Source: SGD
    4. transcription from RNA polymerase II promoter Source: SGD

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29988-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA polymerase II transcription factor B subunit 3
    Alternative name(s):
    RNA polymerase II transcription factor B 38 kDa subunit
    RNA polymerase II transcription factor B p38 subunit
    Gene namesi
    Name:TFB3
    Synonyms:RIG2
    Ordered Locus Names:YDR460W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR460w.
    SGDiS000002868. TFB3.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. holo TFIIH complex Source: SGD
    2. TFIIK complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 321321RNA polymerase II transcription factor B subunit 3PRO_0000055942Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei157 – 1571Phosphoserine1 Publication
    Modified residuei258 – 2581Phosphothreonine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ03290.
    PaxDbiQ03290.
    PeptideAtlasiQ03290.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03290.

    Interactioni

    Subunit structurei

    Component of the transcription factor IIH (TFIIH) holo but not the TFIIH core complex. Component of a complex consisting of KIN28, CCL1 and TFB3; the KIN28-CCL1 dimer is known as the TFIIK complex.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CCL1P373664EBI-31406,EBI-4385

    Protein-protein interaction databases

    BioGridi32514. 25 interactions.
    DIPiDIP-2398N.
    IntActiQ03290. 11 interactions.
    MINTiMINT-528793.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03290.
    SMRiQ03290. Positions 13-75.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri13 – 6048RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5220.
    GeneTreeiENSGT00390000002319.
    HOGENOMiHOG000189680.
    KOiK10842.
    OMAiRIRQKQA.
    OrthoDBiEOG7WMCVV.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR015877. Cdk-activating_kinase_MAT1_cen.
    IPR004575. MAT1/Tfb3.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view]
    PANTHERiPTHR12683. PTHR12683. 1 hit.
    PfamiPF06391. MAT1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003338. MAT1_metazoa. 1 hit.
    SMARTiSM00184. RING. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00570. cdk7. 1 hit.
    PROSITEiPS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03290-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLMDEYEENK DMCPICKTDR YLSPDVKFLV NPECYHRICE SCVDRIFSLG    50
    PAQCPYKGCD KILRKNKFKT QIFDDVEVEK EVDIRKRVFN VFNKTIDDFN 100
    GDLVEYNKYL EEVEDIIYKL DHGIDVAKTE EKLRTYEELN KQLIMNNLER 150
    SRTEIESFEQ RQKFEKEMKL KKRLLERQIE EEERMNKEWT KKEIVNRLST 200
    TTQDINETIE GVKNTVKLKK SSARRKLEEL NRVLKNNPYF NSNVNVQNSR 250
    LKDAVPFTPF NGDREAHPRF TLKGSVYNDP FIKDLEHRKE FIASGFNTNY 300
    AYERVLTEAF MGLGCVISEE L 321
    Length:321
    Mass (Da):38,128
    Last modified:November 1, 1996 - v1
    Checksum:i528D61CF25ADFAF4
    GO

    Sequence cautioni

    The sequence AAB40629.1 differs from that shown. Reason: Frameshift at position 262.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U62805 Genomic DNA. Translation: AAB40629.1. Frameshift.
    U33050 Genomic DNA. Translation: AAB64899.1.
    BK006938 Genomic DNA. Translation: DAA12294.1.
    PIRiS69628.
    RefSeqiNP_010748.3. NM_001180768.3.

    Genome annotation databases

    EnsemblFungiiYDR460W; YDR460W; YDR460W.
    GeneIDi852071.
    KEGGisce:YDR460W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U62805 Genomic DNA. Translation: AAB40629.1 . Frameshift.
    U33050 Genomic DNA. Translation: AAB64899.1 .
    BK006938 Genomic DNA. Translation: DAA12294.1 .
    PIRi S69628.
    RefSeqi NP_010748.3. NM_001180768.3.

    3D structure databases

    ProteinModelPortali Q03290.
    SMRi Q03290. Positions 13-75.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32514. 25 interactions.
    DIPi DIP-2398N.
    IntActi Q03290. 11 interactions.
    MINTi MINT-528793.

    Proteomic databases

    MaxQBi Q03290.
    PaxDbi Q03290.
    PeptideAtlasi Q03290.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR460W ; YDR460W ; YDR460W .
    GeneIDi 852071.
    KEGGi sce:YDR460W.

    Organism-specific databases

    CYGDi YDR460w.
    SGDi S000002868. TFB3.

    Phylogenomic databases

    eggNOGi COG5220.
    GeneTreei ENSGT00390000002319.
    HOGENOMi HOG000189680.
    KOi K10842.
    OMAi RIRQKQA.
    OrthoDBi EOG7WMCVV.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29988-MONOMER.

    Miscellaneous databases

    NextBioi 970364.

    Gene expression databases

    Genevestigatori Q03290.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR015877. Cdk-activating_kinase_MAT1_cen.
    IPR004575. MAT1/Tfb3.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view ]
    PANTHERi PTHR12683. PTHR12683. 1 hit.
    Pfami PF06391. MAT1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003338. MAT1_metazoa. 1 hit.
    SMARTi SM00184. RING. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00570. cdk7. 1 hit.
    PROSITEi PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genes for Tfb2, Tfb3, and Tfb4 subunits of yeast transcription/repair factor IIH. Homology to human cyclin-dependent kinase activating kinase and IIH subunits."
      Feaver W.J., Henry N.L., Wang Z., Wu X., Svejstrup J.Q., Bushnell D.A., Friedberg E.C., Kornberg R.D.
      J. Biol. Chem. 272:19319-19327(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 71-85 AND 236-250, FUNCTION, SUBUNIT, INTERACTION WITH KIN28.
      Strain: DBY2019.
    2. "Rig2, a RING finger protein that interacts with the Kin28/Ccl1 CTD kinase in yeast."
      Faye G., Simon M., Valay J.G., Fesquet D., Facca C.
      Mol. Gen. Genet. 255:460-466(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Sethuraman A., Cherry J.M.
      Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Kin28 is found within TFIIH and a Kin28-Ccl1-Tfb3 trimer complex with differential sensitivities to T-loop phosphorylation."
      Keogh M.-C., Cho E.-J., Podolny V., Buratowski S.
      Mol. Cell. Biol. 22:1288-1297(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COMPLEX WITH KIN28 AND CCL1.
    7. "Revised subunit structure of yeast transcription factor IIH (TFIIH) and reconciliation with human TFIIH."
      Takagi Y., Komori H., Chang W.-H., Hudmon A., Erdjument-Bromage H., Tempst P., Kornberg R.D.
      J. Biol. Chem. 278:43897-43900(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-258, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-157 AND THR-258, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTFB3_YEAST
    AccessioniPrimary (citable) accession number: Q03290
    Secondary accession number(s): D6VT84, P89104
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2005
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3050 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3