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Protein

RNA polymerase II transcription factor B subunit 3

Gene

TFB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the general transcription and DNA repair factor IIH (TFIIH or factor B), which is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. TFIIH has CTD kinase and DNA-dependent ATPase activity, and is essential for polymerase II transcription in vitro.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri13 – 60RING-typePROSITE-ProRule annotationAdd BLAST48

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: InterPro
  • nucleotide-excision repair Source: SGD
  • phosphorylation of RNA polymerase II C-terminal domain Source: SGD
  • regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
  • transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29988-MONOMER.
ReactomeiR-SCE-113418. Formation of the Early Elongation Complex.
R-SCE-5696395. Formation of Incision Complex in GG-NER.
R-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SCE-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-SCE-69202. Cyclin E associated events during G1/S transition.
R-SCE-69273. Cyclin A/B1 associated events during G2/M transition.
R-SCE-69656. Cyclin A:Cdk2-associated events at S phase entry.
R-SCE-72086. mRNA Capping.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SCE-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II transcription factor B subunit 3
Alternative name(s):
RNA polymerase II transcription factor B 38 kDa subunit
RNA polymerase II transcription factor B p38 subunit
Gene namesi
Name:TFB3
Synonyms:RIG2
Ordered Locus Names:YDR460W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR460W.
SGDiS000002868. TFB3.

Subcellular locationi

GO - Cellular componenti

  • holo TFIIH complex Source: SGD
  • TFIIK complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000559421 – 321RNA polymerase II transcription factor B subunit 3Add BLAST321

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei157PhosphoserineCombined sources1
Modified residuei258PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03290.
PRIDEiQ03290.

PTM databases

iPTMnetiQ03290.

Interactioni

Subunit structurei

Component of the transcription factor IIH (TFIIH) holo but not the TFIIH core complex. Component of a complex consisting of KIN28, CCL1 and TFB3; the KIN28-CCL1 dimer is known as the TFIIK complex.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CCL1P373664EBI-31406,EBI-4385

Protein-protein interaction databases

BioGridi32514. 25 interactors.
DIPiDIP-2398N.
IntActiQ03290. 11 interactors.
MINTiMINT-528793.

Structurei

3D structure databases

ProteinModelPortaliQ03290.
SMRiQ03290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri13 – 60RING-typePROSITE-ProRule annotationAdd BLAST48

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000002319.
HOGENOMiHOG000189680.
InParanoidiQ03290.
KOiK10842.
OMAiPANCPVA.
OrthoDBiEOG092C4WQO.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR015877. Cdk-activating_kinase_MAT1_cen.
IPR004575. MAT1/Tfb3.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR12683. PTHR12683. 1 hit.
PfamiPF06391. MAT1. 1 hit.
PF17121. zf-C3HC4_5. 1 hit.
[Graphical view]
PIRSFiPIRSF003338. MAT1_metazoa. 1 hit.
TIGRFAMsiTIGR00570. cdk7. 1 hit.
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03290-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLMDEYEENK DMCPICKTDR YLSPDVKFLV NPECYHRICE SCVDRIFSLG
60 70 80 90 100
PAQCPYKGCD KILRKNKFKT QIFDDVEVEK EVDIRKRVFN VFNKTIDDFN
110 120 130 140 150
GDLVEYNKYL EEVEDIIYKL DHGIDVAKTE EKLRTYEELN KQLIMNNLER
160 170 180 190 200
SRTEIESFEQ RQKFEKEMKL KKRLLERQIE EEERMNKEWT KKEIVNRLST
210 220 230 240 250
TTQDINETIE GVKNTVKLKK SSARRKLEEL NRVLKNNPYF NSNVNVQNSR
260 270 280 290 300
LKDAVPFTPF NGDREAHPRF TLKGSVYNDP FIKDLEHRKE FIASGFNTNY
310 320
AYERVLTEAF MGLGCVISEE L
Length:321
Mass (Da):38,128
Last modified:November 1, 1996 - v1
Checksum:i528D61CF25ADFAF4
GO

Sequence cautioni

The sequence AAB40629 differs from that shown. Reason: Frameshift at position 262.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62805 Genomic DNA. Translation: AAB40629.1. Frameshift.
U33050 Genomic DNA. Translation: AAB64899.1.
BK006938 Genomic DNA. Translation: DAA12294.1.
PIRiS69628.
RefSeqiNP_010748.3. NM_001180768.3.

Genome annotation databases

EnsemblFungiiYDR460W; YDR460W; YDR460W.
GeneIDi852071.
KEGGisce:YDR460W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62805 Genomic DNA. Translation: AAB40629.1. Frameshift.
U33050 Genomic DNA. Translation: AAB64899.1.
BK006938 Genomic DNA. Translation: DAA12294.1.
PIRiS69628.
RefSeqiNP_010748.3. NM_001180768.3.

3D structure databases

ProteinModelPortaliQ03290.
SMRiQ03290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32514. 25 interactors.
DIPiDIP-2398N.
IntActiQ03290. 11 interactors.
MINTiMINT-528793.

PTM databases

iPTMnetiQ03290.

Proteomic databases

MaxQBiQ03290.
PRIDEiQ03290.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR460W; YDR460W; YDR460W.
GeneIDi852071.
KEGGisce:YDR460W.

Organism-specific databases

EuPathDBiFungiDB:YDR460W.
SGDiS000002868. TFB3.

Phylogenomic databases

GeneTreeiENSGT00390000002319.
HOGENOMiHOG000189680.
InParanoidiQ03290.
KOiK10842.
OMAiPANCPVA.
OrthoDBiEOG092C4WQO.

Enzyme and pathway databases

BioCyciYEAST:G3O-29988-MONOMER.
ReactomeiR-SCE-113418. Formation of the Early Elongation Complex.
R-SCE-5696395. Formation of Incision Complex in GG-NER.
R-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SCE-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-SCE-69202. Cyclin E associated events during G1/S transition.
R-SCE-69273. Cyclin A/B1 associated events during G2/M transition.
R-SCE-69656. Cyclin A:Cdk2-associated events at S phase entry.
R-SCE-72086. mRNA Capping.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SCE-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

PROiQ03290.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR015877. Cdk-activating_kinase_MAT1_cen.
IPR004575. MAT1/Tfb3.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR12683. PTHR12683. 1 hit.
PfamiPF06391. MAT1. 1 hit.
PF17121. zf-C3HC4_5. 1 hit.
[Graphical view]
PIRSFiPIRSF003338. MAT1_metazoa. 1 hit.
TIGRFAMsiTIGR00570. cdk7. 1 hit.
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTFB3_YEAST
AccessioniPrimary (citable) accession number: Q03290
Secondary accession number(s): D6VT84, P89104
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3050 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.