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Protein

Agouti-signaling protein

Gene

Asip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the regulation of melanogenesis. The binding of ASP to MC1R precludes alpha-MSH initiated signaling and thus blocks production of cAMP, leading to a down-regulation of eumelanogenesis (brown/black pigment) and thus increasing synthesis of pheomelanin (yellow/red pigment). Causes hair follicle melanocytes to synthesize phaeomelanin instead of black or brown pigment eumelanin and produces hairs with a subapical yellow band on an otherwise black or brown background when expressed during the mid-portion of hair growth.

GO - Molecular functioni

  • melanocortin receptor binding Source: MGI
  • type 3 melanocortin receptor binding Source: MGI
  • type 4 melanocortin receptor binding Source: MGI

GO - Biological processi

  • adult feeding behavior Source: MGI
  • generation of precursor metabolites and energy Source: MGI
  • genetic imprinting Source: MGI
  • hormone-mediated signaling pathway Source: InterPro
  • melanin biosynthetic process Source: MGI
  • melanosome organization Source: MGI
  • melanosome transport Source: MGI
  • pigmentation Source: MGI
  • positive regulation of melanin biosynthetic process Source: CACAO
  • regulation of molecular function, epigenetic Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Agouti-signaling protein
Short name:
ASP
Alternative name(s):
Agouti coat color protein
Agouti switch protein
Gene namesi
Name:Asip
Synonyms:a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:87853. a.

Subcellular locationi

GO - Cellular componenti

  • cell Source: GOC
  • extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 131109Agouti-signaling proteinPRO_0000001029Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi92 ↔ 107PROSITE-ProRule annotation
Disulfide bondi99 ↔ 113PROSITE-ProRule annotation
Disulfide bondi106 ↔ 124PROSITE-ProRule annotation
Disulfide bondi110 ↔ 131PROSITE-ProRule annotation
Disulfide bondi115 ↔ 122PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ03288.

PTM databases

PhosphoSiteiQ03288.

Expressioni

Tissue specificityi

Epithelial cells of the hair follicles and the epidermis.

Developmental stagei

Widely expressed in embryonic and neonatal skin.

Gene expression databases

BgeeiQ03288.
CleanExiMM_A.
ExpressionAtlasiQ03288. baseline and differential.
GenevisibleiQ03288. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029123.

Structurei

3D structure databases

ProteinModelPortaliQ03288.
SMRiQ03288. Positions 92-125.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini92 – 13140AgoutiPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi57 – 8529Arg/Lys-rich (basic)Add
BLAST

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.By similarity

Sequence similaritiesi

Contains 1 agouti domain.PROSITE-ProRule annotation

Keywords - Domaini

Knottin, Signal

Phylogenomic databases

eggNOGiNOG42429.
GeneTreeiENSGT00510000048661.
HOGENOMiHOG000111779.
HOVERGENiHBG050593.
InParanoidiQ03288.
KOiK08725.
OMAiCSCRVLN.
TreeFamiTF330729.

Family and domain databases

Gene3Di4.10.760.10. 1 hit.
InterProiIPR007733. Agouti.
IPR027300. Agouti_dom.
[Graphical view]
PANTHERiPTHR16551. PTHR16551. 1 hit.
PfamiPF05039. Agouti. 1 hit.
[Graphical view]
SMARTiSM00792. Agouti. 1 hit.
[Graphical view]
SUPFAMiSSF57055. SSF57055. 1 hit.
PROSITEiPS60024. AGOUTI_1. 1 hit.
PS51150. AGOUTI_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03288-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVTRLLLAT LVGFLCFFTV HSHLALEETL GDDRSLRSNS SMNSLDFSSV
60 70 80 90 100
SIVALNKKSK KISRKEAEKR KRSSKKKASM KKVARPPPPS PCVATRDSCK
110 120 130
PPAPACCDPC ASCQCRFFGS ACTCRVLNPN C
Length:131
Mass (Da):14,313
Last modified:June 28, 2011 - v2
Checksum:iA7544EEA26734135
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131G → S in AAA37201 (PubMed:8449404).Curated
Sequence conflicti13 – 131G → S in AAA15908 (PubMed:8319910).Curated
Sequence conflicti13 – 131G → S in AAP19635 (PubMed:8319910).Curated
Sequence conflicti13 – 131G → S in AAP19636 (PubMed:8319910).Curated
Sequence conflicti13 – 131G → S in AAP19637 (PubMed:8319910).Curated
Sequence conflicti13 – 131G → S in AAP31517 (PubMed:8319910).Curated
Sequence conflicti13 – 131G → S in AAP31519 (PubMed:8319910).Curated
Sequence conflicti13 – 131G → S in AAP31520 (PubMed:8319910).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06451 mRNA. Translation: AAA37201.1.
L06941 mRNA. No translation available.
AL805955 Genomic DNA. Translation: CAM17068.1.
CH466551 Genomic DNA. Translation: EDL06105.1.
CH466551 Genomic DNA. Translation: EDL06106.1.
BC009122 mRNA. No translation available.
S63413 mRNA. Translation: AAA15908.1.
S70320 mRNA. Translation: AAP19635.1.
S70326 mRNA. Translation: AAP19636.1.
S70394 mRNA. Translation: AAP19637.1.
S74479 mRNA. Translation: AAP31517.1.
S74489 mRNA. Translation: AAP31519.1.
S74493 mRNA. Translation: AAP31520.1.
CCDSiCCDS16941.1.
PIRiA46298.
RefSeqiNP_056585.2. NM_015770.3.
UniGeneiMm.315593.
Mm.488809.

Genome annotation databases

EnsembliENSMUST00000029123; ENSMUSP00000029123; ENSMUSG00000027596.
ENSMUST00000109697; ENSMUSP00000105319; ENSMUSG00000027596.
GeneIDi50518.
KEGGimmu:50518.
UCSCiuc008njz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06451 mRNA. Translation: AAA37201.1.
L06941 mRNA. No translation available.
AL805955 Genomic DNA. Translation: CAM17068.1.
CH466551 Genomic DNA. Translation: EDL06105.1.
CH466551 Genomic DNA. Translation: EDL06106.1.
BC009122 mRNA. No translation available.
S63413 mRNA. Translation: AAA15908.1.
S70320 mRNA. Translation: AAP19635.1.
S70326 mRNA. Translation: AAP19636.1.
S70394 mRNA. Translation: AAP19637.1.
S74479 mRNA. Translation: AAP31517.1.
S74489 mRNA. Translation: AAP31519.1.
S74493 mRNA. Translation: AAP31520.1.
CCDSiCCDS16941.1.
PIRiA46298.
RefSeqiNP_056585.2. NM_015770.3.
UniGeneiMm.315593.
Mm.488809.

3D structure databases

ProteinModelPortaliQ03288.
SMRiQ03288. Positions 92-125.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029123.

PTM databases

PhosphoSiteiQ03288.

Proteomic databases

PRIDEiQ03288.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029123; ENSMUSP00000029123; ENSMUSG00000027596.
ENSMUST00000109697; ENSMUSP00000105319; ENSMUSG00000027596.
GeneIDi50518.
KEGGimmu:50518.
UCSCiuc008njz.1. mouse.

Organism-specific databases

CTDi50518.
MGIiMGI:87853. a.

Phylogenomic databases

eggNOGiNOG42429.
GeneTreeiENSGT00510000048661.
HOGENOMiHOG000111779.
HOVERGENiHBG050593.
InParanoidiQ03288.
KOiK08725.
OMAiCSCRVLN.
TreeFamiTF330729.

Miscellaneous databases

NextBioi307514.
PROiQ03288.
SOURCEiSearch...

Gene expression databases

BgeeiQ03288.
CleanExiMM_A.
ExpressionAtlasiQ03288. baseline and differential.
GenevisibleiQ03288. MM.

Family and domain databases

Gene3Di4.10.760.10. 1 hit.
InterProiIPR007733. Agouti.
IPR027300. Agouti_dom.
[Graphical view]
PANTHERiPTHR16551. PTHR16551. 1 hit.
PfamiPF05039. Agouti. 1 hit.
[Graphical view]
SMARTiSM00792. Agouti. 1 hit.
[Graphical view]
SUPFAMiSSF57055. SSF57055. 1 hit.
PROSITEiPS60024. AGOUTI_1. 1 hit.
PS51150. AGOUTI_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the mouse agouti gene predicts a secreted protein ubiquitously expressed in mice carrying the lethal yellow mutation."
    Miller M.W., Duhl D.M., Vrieling H., Cordes S.P., Ollmann M.M., Winkes B.M., Barsh G.S.
    Genes Dev. 7:454-467(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular characterization of the mouse agouti locus."
    Bultman S.J., Michaud E.J., Woychik R.P.
    Cell 71:1195-1204(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: 129.
    Tissue: Mammary gland.
  6. "The embryonic lethality of homozygous lethal yellow mice (Ay/Ay) is associated with the disruption of a novel RNA-binding protein."
    Michaud E.J., Bultman S.J., Stubbs L.J., Woychik R.P.
    Genes Dev. 7:1203-1213(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-70.
  7. "Neomorphic agouti mutations in obese yellow mice."
    Duhl D.M., Vrieling H., Miller K.A., Wolff G.L., Barsh G.S.
    Nat. Genet. 8:59-65(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-16.
    Strain: C57BL/6J.
    Tissue: Spleen.
  8. "Differences in dorsal and ventral pigmentation result from regional expression of the mouse agouti gene."
    Vrieling H., Duhl D.M., Millar S.E., Miller K.A., Barsh G.S.
    Proc. Natl. Acad. Sci. U.S.A. 91:5667-5671(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-16.

Entry informationi

Entry nameiASIP_MOUSE
AccessioniPrimary (citable) accession number: Q03288
Secondary accession number(s): A2ALT3, Q80ST0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 28, 2011
Last modified: June 24, 2015
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.