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Q03288 (ASIP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Agouti-signaling protein

Short name=ASP
Alternative name(s):
Agouti coat color protein
Agouti switch protein
Gene names
Name:Asip
Synonyms:a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length131 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the regulation of melanogenesis. The binding of ASP to MC1R precludes alpha-MSH initiated signaling and thus blocks production of cAMP, leading to a down-regulation of eumelanogenesis (brown/black pigment) and thus increasing synthesis of pheomelanin (yellow/red pigment). Causes hair follicle melanocytes to synthesize phaeomelanin instead of black or brown pigment eumelanin and produces hairs with a subapical yellow band on an otherwise black or brown background when expressed during the mid-portion of hair growth.

Subcellular location

Secreted.

Tissue specificity

Epithelial cells of the hair follicles and the epidermis.

Developmental stage

Widely expressed in embryonic and neonatal skin.

Domain

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin By similarity.

Sequence similarities

Contains 1 agouti domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 131109Agouti-signaling protein
PRO_0000001029

Regions

Domain92 – 13140Agouti
Compositional bias57 – 8529Arg/Lys-rich (basic)

Amino acid modifications

Glycosylation391N-linked (GlcNAc...) Potential
Disulfide bond92 ↔ 107 By similarity
Disulfide bond99 ↔ 113 By similarity
Disulfide bond106 ↔ 124 By similarity
Disulfide bond110 ↔ 131 By similarity
Disulfide bond115 ↔ 122 By similarity

Experimental info

Sequence conflict131G → S in AAA37201. Ref.1
Sequence conflict131G → S in AAA15908. Ref.6
Sequence conflict131G → S in AAP19635. Ref.6
Sequence conflict131G → S in AAP19636. Ref.6
Sequence conflict131G → S in AAP19637. Ref.6
Sequence conflict131G → S in AAP31517. Ref.6
Sequence conflict131G → S in AAP31519. Ref.6
Sequence conflict131G → S in AAP31520. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q03288 [UniParc].

Last modified June 28, 2011. Version 2.
Checksum: A7544EEA26734135

FASTA13114,313
        10         20         30         40         50         60 
MDVTRLLLAT LVGFLCFFTV HSHLALEETL GDDRSLRSNS SMNSLDFSSV SIVALNKKSK 

        70         80         90        100        110        120 
KISRKEAEKR KRSSKKKASM KKVARPPPPS PCVATRDSCK PPAPACCDPC ASCQCRFFGS 

       130 
ACTCRVLNPN C 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of the mouse agouti gene predicts a secreted protein ubiquitously expressed in mice carrying the lethal yellow mutation."
Miller M.W., Duhl D.M., Vrieling H., Cordes S.P., Ollmann M.M., Winkes B.M., Barsh G.S.
Genes Dev. 7:454-467(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular characterization of the mouse agouti locus."
Bultman S.J., Michaud E.J., Woychik R.P.
Cell 71:1195-1204(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: 129.
Tissue: Mammary gland.
[6]"The embryonic lethality of homozygous lethal yellow mice (Ay/Ay) is associated with the disruption of a novel RNA-binding protein."
Michaud E.J., Bultman S.J., Stubbs L.J., Woychik R.P.
Genes Dev. 7:1203-1213(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-70.
[7]"Neomorphic agouti mutations in obese yellow mice."
Duhl D.M., Vrieling H., Miller K.A., Wolff G.L., Barsh G.S.
Nat. Genet. 8:59-65(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-16.
Strain: C57BL/6J.
Tissue: Spleen.
[8]"Differences in dorsal and ventral pigmentation result from regional expression of the mouse agouti gene."
Vrieling H., Duhl D.M., Millar S.E., Miller K.A., Barsh G.S.
Proc. Natl. Acad. Sci. U.S.A. 91:5667-5671(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-16.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L06451 mRNA. Translation: AAA37201.1.
L06941 mRNA. No translation available.
AL805955 Genomic DNA. Translation: CAM17068.1.
CH466551 Genomic DNA. Translation: EDL06105.1.
CH466551 Genomic DNA. Translation: EDL06106.1.
BC009122 mRNA. No translation available.
S63413 mRNA. Translation: AAA15908.1.
S70320 mRNA. Translation: AAP19635.1.
S70326 mRNA. Translation: AAP19636.1.
S70394 mRNA. Translation: AAP19637.1.
S74479 mRNA. Translation: AAP31517.1.
S74489 mRNA. Translation: AAP31519.1.
S74493 mRNA. Translation: AAP31520.1.
CCDSCCDS16941.1.
PIRA46298.
RefSeqNP_056585.2. NM_015770.3.
XP_006499942.1. XM_006499879.1.
UniGeneMm.315593.
Mm.488809.

3D structure databases

ProteinModelPortalQ03288.
SMRQ03288. Positions 92-125.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000105319.

PTM databases

PhosphoSiteQ03288.

Proteomic databases

PRIDEQ03288.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000029123; ENSMUSP00000029123; ENSMUSG00000027596.
ENSMUST00000109697; ENSMUSP00000105319; ENSMUSG00000027596.
GeneID50518.
KEGGmmu:50518.
UCSCuc008njz.1. mouse.

Organism-specific databases

CTD50518.
MGIMGI:87853. a.

Phylogenomic databases

eggNOGNOG42429.
GeneTreeENSGT00510000048661.
HOGENOMHOG000111779.
HOVERGENHBG050593.
InParanoidA2ALT3.
KOK08725.
OMACSCRVLN.
TreeFamTF330729.

Gene expression databases

ArrayExpressQ03288.
BgeeQ03288.
CleanExMM_A.
GenevestigatorQ03288.

Family and domain databases

Gene3D4.10.760.10. 1 hit.
InterProIPR007733. Agouti.
IPR027300. Agouti_dom.
[Graphical view]
PANTHERPTHR16551. PTHR16551. 1 hit.
PfamPF05039. Agouti. 1 hit.
[Graphical view]
SMARTSM00792. Agouti. 1 hit.
[Graphical view]
SUPFAMSSF57055. SSF57055. 1 hit.
PROSITEPS60024. AGOUTI_1. 1 hit.
PS51150. AGOUTI_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio307514.
PROQ03288.
SOURCESearch...

Entry information

Entry nameASIP_MOUSE
AccessionPrimary (citable) accession number: Q03288
Secondary accession number(s): A2ALT3, Q80ST0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 28, 2011
Last modified: July 9, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot