Q03288 (ASIP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Agouti-signaling protein
Short name=ASP

Alternative name(s):
Agouti coat color protein
Agouti switch protein

Gene names

Name:	Asip
Synonyms:	a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the regulation of melanogenesis. The binding of ASP to MC1R precludes alpha-MSH initiated signaling and thus blocks production of cAMP, leading to a down-regulation of eumelanogenesis (brown/black pigment) and thus increasing synthesis of pheomelanin (yellow/red pigment). Causes hair follicle melanocytes to synthesize phaeomelanin instead of black or brown pigment eumelanin and produces hairs with a subapical yellow band on an otherwise black or brown background when expressed during the mid-portion of hair growth.
Subcellular location	Secreted.
Tissue specificity	Epithelial cells of the hair follicles and the epidermis.
Developmental stage	Widely expressed in embryonic and neonatal skin.
Domain	The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin By similarity.
Sequence similarities	Contains 1 agouti domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Knottin Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adult feeding behavior Inferred from mutant phenotype PubMed 17873059. Source: MGI generation of precursor metabolites and energy Inferred from genetic interaction PubMed 12177191. Source: MGI genetic imprinting Inferred from mutant phenotype PubMed 12601169. Source: MGI hormone-mediated signaling pathway Inferred from electronic annotation. Source: InterPro melanin biosynthetic process Inferred from mutant phenotype PubMed 7665913. Source: MGI melanosome organization Inferred from mutant phenotype PubMed 9548375. Source: MGI melanosome transport Inferred from mutant phenotype PubMed 9548375. Source: MGI regulation of molecular function, epigenetic Inferred from mutant phenotype PubMed 12601169. Source: MGI
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 131	109	Agouti-signaling protein		PRO_0000001029
Regions
	Domain	92 – 131	40	Agouti
	Compositional bias	57 – 85	29	Arg/Lys-rich (basic)
Amino acid modifications
	Glycosylation	39	1	N-linked (GlcNAc...) Potential
	Disulfide bond	92 ↔ 107		By similarity
	Disulfide bond	99 ↔ 113		By similarity
	Disulfide bond	106 ↔ 124		By similarity
	Disulfide bond	110 ↔ 131		By similarity
	Disulfide bond	115 ↔ 122		By similarity
Experimental info
	Sequence conflict	13	1	G → S in AAA37201. Ref.1
	Sequence conflict	13	1	G → S in AAA15908. Ref.6
	Sequence conflict	13	1	G → S in AAP19635. Ref.6
	Sequence conflict	13	1	G → S in AAP19636. Ref.6
	Sequence conflict	13	1	G → S in AAP19637. Ref.6
	Sequence conflict	13	1	G → S in AAP31517. Ref.6
	Sequence conflict	13	1	G → S in AAP31519. Ref.6
	Sequence conflict	13	1	G → S in AAP31520. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q03288 [UniParc]. Last modified June 28, 2011. Version 2. Checksum: A7544EEA26734135	FASTA	131	14,313
	10 20 30 40 50 60 MDVTRLLLAT LVGFLCFFTV HSHLALEETL GDDRSLRSNS SMNSLDFSSV SIVALNKKSK 70 80 90 100 110 120 KISRKEAEKR KRSSKKKASM KKVARPPPPS PCVATRDSCK PPAPACCDPC ASCQCRFFGS 130 ACTCRVLNPN C

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the mouse agouti gene predicts a secreted protein ubiquitously expressed in mice carrying the lethal yellow mutation." Miller M.W., Duhl D.M., Vrieling H., Cordes S.P., Ollmann M.M., Winkes B.M., Barsh G.S. Genes Dev. 7:454-467(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular characterization of the mouse agouti locus." Bultman S.J., Michaud E.J., Woychik R.P. Cell 71:1195-1204(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: 129. Tissue: Mammary gland.
[6]	"The embryonic lethality of homozygous lethal yellow mice (Ay/Ay) is associated with the disruption of a novel RNA-binding protein." Michaud E.J., Bultman S.J., Stubbs L.J., Woychik R.P. Genes Dev. 7:1203-1213(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-70.
[7]	"Neomorphic agouti mutations in obese yellow mice." Duhl D.M., Vrieling H., Miller K.A., Wolff G.L., Barsh G.S. Nat. Genet. 8:59-65(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-16. Strain: C57BL/6J. Tissue: Spleen.
[8]	"Differences in dorsal and ventral pigmentation result from regional expression of the mouse agouti gene." Vrieling H., Duhl D.M., Millar S.E., Miller K.A., Barsh G.S. Proc. Natl. Acad. Sci. U.S.A. 91:5667-5671(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-16.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L06451 mRNA. Translation: AAA37201.1. L06941 mRNA. No translation available. AL805955 Genomic DNA. Translation: CAM17068.1. CH466551 Genomic DNA. Translation: EDL06105.1. CH466551 Genomic DNA. Translation: EDL06106.1. BC009122 mRNA. No translation available. S63413 mRNA. Translation: AAA15908.1. S70320 mRNA. Translation: AAP19635.1. S70326 mRNA. Translation: AAP19636.1. S70394 mRNA. Translation: AAP19637.1. S74479 mRNA. Translation: AAP31517.1. S74489 mRNA. Translation: AAP31519.1. S74493 mRNA. Translation: AAP31520.1.
IPI	IPI00130315.
PIR	A46298.
RefSeq	NP_056585.2. NM_015770.3.
UniGene	Mm.221440. Mm.315593.
3D structure databases
ProteinModelPortal	Q03288.
SMR	Q03288. Positions 92-125.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000105319.
PTM databases
PhosphoSite	Q03288.
Proteomic databases
PRIDE	Q03288.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000029123; ENSMUSP00000029123; ENSMUSG00000027596. ENSMUST00000109697; ENSMUSP00000105319; ENSMUSG00000027596.
GeneID	50518.
KEGG	mmu:50518.
Organism-specific databases
CTD	50518.
MGI	MGI:87853. a.
Phylogenomic databases
eggNOG	NOG42429.
GeneTree	ENSGT00510000048661.
HOGENOM	HOG000111779.
HOVERGEN	HBG050593.
InParanoid	A2ALT3.
KO	K08725.
OMA	CSCRVLN.
OrthoDB	EOG4907BH.
Gene expression databases
ArrayExpress	Q03288.
Bgee	Q03288.
CleanEx	MM_A.
Genevestigator	Q03288.
GermOnline	ENSMUSG00000027596. Mus musculus.
Family and domain databases
Gene3D	4.10.760.10. 1 hit.
InterPro	IPR007733. Agouti. IPR027300. Agouti_dom. [Graphical view]
PANTHER	PTHR16551. PTHR16551. 1 hit.
Pfam	PF05039. Agouti. 1 hit. [Graphical view]
SMART	SM00792. Agouti. 1 hit. [Graphical view]
SUPFAM	SSF57055. Agouti. 1 hit.
PROSITE	PS60024. AGOUTI_1. 1 hit. PS51150. AGOUTI_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	307514.
SOURCE	Search...

Entry information

Entry name

ASIP_MOUSE

Accession

Primary (citable) accession number: Q03288
Secondary accession number(s): A2ALT3, Q80ST0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	June 28, 2011
Last modified:	May 1, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program