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Q03288

- ASIP_MOUSE

UniProt

Q03288 - ASIP_MOUSE

Protein

Agouti-signaling protein

Gene

Asip

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Involved in the regulation of melanogenesis. The binding of ASP to MC1R precludes alpha-MSH initiated signaling and thus blocks production of cAMP, leading to a down-regulation of eumelanogenesis (brown/black pigment) and thus increasing synthesis of pheomelanin (yellow/red pigment). Causes hair follicle melanocytes to synthesize phaeomelanin instead of black or brown pigment eumelanin and produces hairs with a subapical yellow band on an otherwise black or brown background when expressed during the mid-portion of hair growth.

    GO - Molecular functioni

    1. melanocortin receptor binding Source: MGI
    2. protein binding Source: MGI
    3. type 3 melanocortin receptor binding Source: MGI
    4. type 4 melanocortin receptor binding Source: MGI

    GO - Biological processi

    1. adult feeding behavior Source: MGI
    2. generation of precursor metabolites and energy Source: MGI
    3. genetic imprinting Source: MGI
    4. hormone-mediated signaling pathway Source: InterPro
    5. melanin biosynthetic process Source: MGI
    6. melanosome organization Source: MGI
    7. melanosome transport Source: MGI
    8. pigmentation Source: MGI
    9. regulation of molecular function, epigenetic Source: MGI

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Agouti-signaling protein
    Short name:
    ASP
    Alternative name(s):
    Agouti coat color protein
    Agouti switch protein
    Gene namesi
    Name:Asip
    Synonyms:a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:87853. a.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 131109Agouti-signaling proteinPRO_0000001029Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi39 – 391N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi92 ↔ 107PROSITE-ProRule annotation
    Disulfide bondi99 ↔ 113PROSITE-ProRule annotation
    Disulfide bondi106 ↔ 124PROSITE-ProRule annotation
    Disulfide bondi110 ↔ 131PROSITE-ProRule annotation
    Disulfide bondi115 ↔ 122PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ03288.

    PTM databases

    PhosphoSiteiQ03288.

    Expressioni

    Tissue specificityi

    Epithelial cells of the hair follicles and the epidermis.

    Developmental stagei

    Widely expressed in embryonic and neonatal skin.

    Gene expression databases

    ArrayExpressiQ03288.
    BgeeiQ03288.
    CleanExiMM_A.
    GenevestigatoriQ03288.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000105319.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03288.
    SMRiQ03288. Positions 92-125.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini92 – 13140AgoutiPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi57 – 8529Arg/Lys-rich (basic)Add
    BLAST

    Domaini

    The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.By similarity

    Sequence similaritiesi

    Contains 1 agouti domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Knottin, Signal

    Phylogenomic databases

    eggNOGiNOG42429.
    GeneTreeiENSGT00510000048661.
    HOGENOMiHOG000111779.
    HOVERGENiHBG050593.
    InParanoidiA2ALT3.
    KOiK08725.
    OMAiCSCRVLN.
    TreeFamiTF330729.

    Family and domain databases

    Gene3Di4.10.760.10. 1 hit.
    InterProiIPR007733. Agouti.
    IPR027300. Agouti_dom.
    [Graphical view]
    PANTHERiPTHR16551. PTHR16551. 1 hit.
    PfamiPF05039. Agouti. 1 hit.
    [Graphical view]
    SMARTiSM00792. Agouti. 1 hit.
    [Graphical view]
    SUPFAMiSSF57055. SSF57055. 1 hit.
    PROSITEiPS60024. AGOUTI_1. 1 hit.
    PS51150. AGOUTI_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q03288-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDVTRLLLAT LVGFLCFFTV HSHLALEETL GDDRSLRSNS SMNSLDFSSV    50
    SIVALNKKSK KISRKEAEKR KRSSKKKASM KKVARPPPPS PCVATRDSCK 100
    PPAPACCDPC ASCQCRFFGS ACTCRVLNPN C 131
    Length:131
    Mass (Da):14,313
    Last modified:June 28, 2011 - v2
    Checksum:iA7544EEA26734135
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131G → S in AAA37201. (PubMed:8449404)Curated
    Sequence conflicti13 – 131G → S in AAA15908. (PubMed:8319910)Curated
    Sequence conflicti13 – 131G → S in AAP19635. (PubMed:8319910)Curated
    Sequence conflicti13 – 131G → S in AAP19636. (PubMed:8319910)Curated
    Sequence conflicti13 – 131G → S in AAP19637. (PubMed:8319910)Curated
    Sequence conflicti13 – 131G → S in AAP31517. (PubMed:8319910)Curated
    Sequence conflicti13 – 131G → S in AAP31519. (PubMed:8319910)Curated
    Sequence conflicti13 – 131G → S in AAP31520. (PubMed:8319910)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06451 mRNA. Translation: AAA37201.1.
    L06941 mRNA. No translation available.
    AL805955 Genomic DNA. Translation: CAM17068.1.
    CH466551 Genomic DNA. Translation: EDL06105.1.
    CH466551 Genomic DNA. Translation: EDL06106.1.
    BC009122 mRNA. No translation available.
    S63413 mRNA. Translation: AAA15908.1.
    S70320 mRNA. Translation: AAP19635.1.
    S70326 mRNA. Translation: AAP19636.1.
    S70394 mRNA. Translation: AAP19637.1.
    S74479 mRNA. Translation: AAP31517.1.
    S74489 mRNA. Translation: AAP31519.1.
    S74493 mRNA. Translation: AAP31520.1.
    CCDSiCCDS16941.1.
    PIRiA46298.
    RefSeqiNP_056585.2. NM_015770.3.
    XP_006499942.1. XM_006499879.1.
    UniGeneiMm.315593.
    Mm.488809.

    Genome annotation databases

    EnsembliENSMUST00000029123; ENSMUSP00000029123; ENSMUSG00000027596.
    ENSMUST00000109697; ENSMUSP00000105319; ENSMUSG00000027596.
    GeneIDi50518.
    KEGGimmu:50518.
    UCSCiuc008njz.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06451 mRNA. Translation: AAA37201.1 .
    L06941 mRNA. No translation available.
    AL805955 Genomic DNA. Translation: CAM17068.1 .
    CH466551 Genomic DNA. Translation: EDL06105.1 .
    CH466551 Genomic DNA. Translation: EDL06106.1 .
    BC009122 mRNA. No translation available.
    S63413 mRNA. Translation: AAA15908.1 .
    S70320 mRNA. Translation: AAP19635.1 .
    S70326 mRNA. Translation: AAP19636.1 .
    S70394 mRNA. Translation: AAP19637.1 .
    S74479 mRNA. Translation: AAP31517.1 .
    S74489 mRNA. Translation: AAP31519.1 .
    S74493 mRNA. Translation: AAP31520.1 .
    CCDSi CCDS16941.1.
    PIRi A46298.
    RefSeqi NP_056585.2. NM_015770.3.
    XP_006499942.1. XM_006499879.1.
    UniGenei Mm.315593.
    Mm.488809.

    3D structure databases

    ProteinModelPortali Q03288.
    SMRi Q03288. Positions 92-125.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000105319.

    PTM databases

    PhosphoSitei Q03288.

    Proteomic databases

    PRIDEi Q03288.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029123 ; ENSMUSP00000029123 ; ENSMUSG00000027596 .
    ENSMUST00000109697 ; ENSMUSP00000105319 ; ENSMUSG00000027596 .
    GeneIDi 50518.
    KEGGi mmu:50518.
    UCSCi uc008njz.1. mouse.

    Organism-specific databases

    CTDi 50518.
    MGIi MGI:87853. a.

    Phylogenomic databases

    eggNOGi NOG42429.
    GeneTreei ENSGT00510000048661.
    HOGENOMi HOG000111779.
    HOVERGENi HBG050593.
    InParanoidi A2ALT3.
    KOi K08725.
    OMAi CSCRVLN.
    TreeFami TF330729.

    Miscellaneous databases

    NextBioi 307514.
    PROi Q03288.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q03288.
    Bgeei Q03288.
    CleanExi MM_A.
    Genevestigatori Q03288.

    Family and domain databases

    Gene3Di 4.10.760.10. 1 hit.
    InterProi IPR007733. Agouti.
    IPR027300. Agouti_dom.
    [Graphical view ]
    PANTHERi PTHR16551. PTHR16551. 1 hit.
    Pfami PF05039. Agouti. 1 hit.
    [Graphical view ]
    SMARTi SM00792. Agouti. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57055. SSF57055. 1 hit.
    PROSITEi PS60024. AGOUTI_1. 1 hit.
    PS51150. AGOUTI_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the mouse agouti gene predicts a secreted protein ubiquitously expressed in mice carrying the lethal yellow mutation."
      Miller M.W., Duhl D.M., Vrieling H., Cordes S.P., Ollmann M.M., Winkes B.M., Barsh G.S.
      Genes Dev. 7:454-467(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Molecular characterization of the mouse agouti locus."
      Bultman S.J., Michaud E.J., Woychik R.P.
      Cell 71:1195-1204(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: 129.
      Tissue: Mammary gland.
    6. "The embryonic lethality of homozygous lethal yellow mice (Ay/Ay) is associated with the disruption of a novel RNA-binding protein."
      Michaud E.J., Bultman S.J., Stubbs L.J., Woychik R.P.
      Genes Dev. 7:1203-1213(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-70.
    7. "Neomorphic agouti mutations in obese yellow mice."
      Duhl D.M., Vrieling H., Miller K.A., Wolff G.L., Barsh G.S.
      Nat. Genet. 8:59-65(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-16.
      Strain: C57BL/6J.
      Tissue: Spleen.
    8. "Differences in dorsal and ventral pigmentation result from regional expression of the mouse agouti gene."
      Vrieling H., Duhl D.M., Millar S.E., Miller K.A., Barsh G.S.
      Proc. Natl. Acad. Sci. U.S.A. 91:5667-5671(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-16.

    Entry informationi

    Entry nameiASIP_MOUSE
    AccessioniPrimary (citable) accession number: Q03288
    Secondary accession number(s): A2ALT3, Q80ST0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3