Reviewed, UniProtKB/Swiss-Prot Q03278 (PO21_NASVI)

Names and origin

Protein names	Recommended name:     Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 Alternative name(s):     Retrovirus-related Pol polyprotein from type I retrotransposable element R2 Including the following 2 domains:     1- Recommended name:             Reverse transcriptase               EC=2.7.7.49     2- Recommended name:             Endonuclease
Organism	Nasonia vitripennis (Parasitic wasp)
Taxonomic identifier	7425 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Chalcidoidea › Pteromalidae › Pteromalinae › Nasonia

Protein attributes

Sequence length	1025 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Sequence similarities	Contains 1 C2H2-type zinc finger. Contains 1 reverse transcriptase domain.

Ontologies

Keywords
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Endonuclease Hydrolase Nuclease Nucleotidyltransferase RNA-directed DNA polymerase Transferase
Technical term	Transposable element
Gene Ontology (GO)
Biological process	RNA-dependent DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 1025	›1025	Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2		PRO_0000058497
Regions
	Domain	358 – 635	278	Reverse transcriptase
	Zinc finger	46 – 69	24	C2H2-type
	Region	755 – 1025	271	Nucleic acid-binding endonuclease
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q03278-1 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 387BDE63BCF5C518	FASTA	1,025	115,885
	10 20 30 40 50 60 NQIKKSNTST GARIPKAMTN PADNFAGGQW KPPGRRSART SATGMFVCEH CLRAFTTNTG 70 80 90 100 110 120 RGLHIKRAHE EQANEAITTE RSRARWTNEE MEAVQAEIDC EGRTAINQEI LRIIPYQRTI 130 140 150 160 170 180 DAIKCLRKQQ KYKTIRERVA NRRAENRARE TELTRLETAD EDPASQEQDN PNMSLKNWLK 190 200 210 220 230 240 EVIESDDDRL CADLRTAIEM ALAGQSPLDV CTVGCYQYTM TNLPLVPVRL GGPIYWCNAQ 250 260 270 280 290 300 SRSNPGETQR RQTIKESNNS WKKNMSKAAH IVLDGDTDAC PAGLEGTEAS GAIMRAGCPT 310 320 330 340 350 360 TRHLRSRMQG EIKNLWRPIS NDEIKEVEAC KRTAAGPDGM TTTAWNSIDE CIKSLFNMIM 370 380 390 400 410 420 YHGQCPRRYL DSRTVLIPKE PGTMDPACFR PLSIASVALR HFHRILANRI GEHGLLDTRQ 430 440 450 460 470 480 RAFIVADGVA ENTSLLSAMI KEARMKIKGL YIAILDVKKA FDSVEHRSIL DALRRKKLPL 490 500 510 520 530 540 EMRNYIMWVY RNSKTRLEVV KTKGRWIRPA RGVRQGDPLS PLLFNCVMDA VLRRLPENTG 550 560 570 580 590 600 FLMGAEKIGA LVFADDLVLL AETREGLQAS LSRIEAGLQE QGLEMMPRKC HTLALVPSGK 610 620 630 640 650 660 EKKIKVETHK PFTVGNQEIT QLGHADQWKY LGVVYNSYGP IQVKINIAGD LQRVTAAPLK 670 680 690 700 710 720 PQQRMAILGM FLIPRFIHKL VLGRTSNADV RKGDKIIRKT VRGWLRLPHD TPIGYFHAPI 730 740 750 760 770 780 KEGGLGIPAF ESRIPELLKS RIEALGASNM QTARSLLGGD WVAERKKWIN TQKIKNSEWA 790 800 810 820 830 840 QKLHLTTDGK DLRDTRKAEA SYSWIRDIHV AIPASVWIKY HHTRINALPT LMRMSRGRRT 850 860 870 880 890 900 NGNALCRAGC GLPETLYHVV QQCPRTHGGR VLRHDKIAEQ VAIFMQEKGW LVLREAHIRT 910 920 930 940 950 960 SVGLRKPDII ARKGQDCKII DCQIVTTGND IRIQHERKIQ YYASNWELRR SAATMIGHQG 970 980 990 1000 1010 1020 QVSVEAITIS WKGVWEPRSY CLLRDCGIPK VKIKGLTTRV LLGAYLNFNT FSKATYRTER RRTAN

References

[1]	"Sequence relationship of retrotransposable elements R1 and R2 within and between divergent insect species." Burke W.D., Eickbush D.G., Xiong Y., Jakubczak J.L., Eickbush T.H. Mol. Biol. Evol. 10:163-185(1993) [PubMed: 8383793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Burke W.D., Eickbush D.G., Xiong Y., Jakubczak J.L., Eickbush T.H. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L00950 Genomic DNA. Translation: AAC34927.1.
PIR	T10259.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.7.49. 294130.
Family and domain databases
InterPro	IPR000477. Reverse_transcriptase. IPR015706. Reverse_transcriptase-related. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. [Graphical view]
PANTHER	PTHR19446. Rev_txptase. 1 hit.
Pfam	PF00078. RVT_1. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 1 hit. [Graphical view]
PROSITE	PS50878. RT_POL. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PO21_NASVI

Accession

Primary (citable) accession number: Q03278

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	July 15, 1998
Last modified:	September 22, 2009
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)