Reviewed, UniProtKB/Swiss-Prot Q03274 (PO22_POPJA)

Names and origin

Protein names	Recommended name:     Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 Alternative name(s):     Retrovirus-related Pol polyprotein from type I retrotransposable element R2 Including the following 2 domains:     1- Recommended name:             Reverse transcriptase               EC=2.7.7.49     2- Recommended name:             Endonuclease
Organism	Popillia japonica (Japanese beetle)
Taxonomic identifier	7064 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Coleoptera › Polyphaga › Scarabaeiformia › Scarabaeidae › Rutelinae › Popillia

Protein attributes

Sequence length	711 AA.
Sequence status	Fragment.
Protein existence	Predicted.

General annotation (Comments)

Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Sequence similarities	Contains 1 reverse transcriptase domain.

Ontologies

Keywords
Molecular function	Endonuclease Hydrolase Nuclease Nucleotidyltransferase RNA-directed DNA polymerase Transferase
Technical term	Transposable element
Gene Ontology (GO)
Biological process	RNA-dependent DNA replication Inferred from electronic annotation. Source: InterPro
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 711	›711	Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2		PRO_0000058500
Regions
	Domain	45 – 323	279	Reverse transcriptase
	Region	444 – 711	268	Nucleic acid-binding endonuclease
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q03274-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: EAA25C9085F12D04	FASTA	711	79,459
	10 20 30 40 50 60 HTYRPIAREE IQCAIKGWKP SAPGSDGLTV QAITRTRLPR NFVQLHLLRG HVPTPWTAMR 70 80 90 100 110 120 TTLIPKDGDL ENPSNWRPIT IASALQRLLH RILAKRLEAA VELHPAQKGY ARIDGTLVNS 130 140 150 160 170 180 LLLDTYISSR REQRKTYNVV SLDVRKAFDT VSHSSICRAL QRLGIDEGTS NYITGSLSDS 190 200 210 220 230 240 TTTIRVGPGS QTRKICIRRG VKQGDPLSPF LFNAVLDELL CSLQSTPGIG GTIGEEKIPV 250 260 270 280 290 300 LAFADDLLLL EDNDVLLPTT LATVANFFRL RGMSLNAKKS VSISVAASGG VCIPRTKPFL 310 320 330 340 350 360 RVDNVLLPVT DRMQTFRYLG HFFGLSGAAK PTVYNLSRWL KCVEAAPLKP EQKLSLIREH 370 380 390 400 410 420 VVPKLLYGLQ NPSVTARTLR DADKLIRTTV KRCLHLHLHT PNQCFYARVR DGGLGLTDLR 430 440 450 460 470 480 RSIPRIMLDR INARRSSDPM AVALFSCPSF DHLRGRLVAL AGDVPPSHFW REAIANHTTT 490 500 510 520 530 540 KGLEAASDDP ASRSWIFRKP YGWSGKDFVR AIHLRTGNLP TKAIPSNPAG ERLCRGGCGK 550 560 570 580 590 600 QATISHVLQR CPVVQPERIR RHNEIARKIA AHCRSKGWTV EEEPHIRHPL GHLYKPDIVI 610 620 630 640 650 660 HREGLPSVVC DVQVSWDGYE PLDEAWRNKQ RTYDHDLFRT AAGRRWPGKT FLHLPAILGA 670 680 690 700 710 RGIWPRCNSE TASALAFTNQ LKASWLHSCL KWDSTLHTSF MRAVWSRQAG A

References

[1]	"Sequence relationship of retrotransposable elements R1 and R2 within and between divergent insect species." Burke W.D., Eickbush D.G., Xiong Y., Jakubczak J.L., Eickbush T.H. Mol. Biol. Evol. 10:163-185(1993) [PubMed: 8383793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Retrotransposable elements R1 and R2 interrupt the rRNA genes of most insects." Jakubczak J.L., Burke W.D., Eickbush T.H. Proc. Natl. Acad. Sci. U.S.A. 88:3295-3299(1991) [PubMed: 1849649] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 534-551.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L00947 Genomic DNA. Translation: AAA29785.1.
PIR	H44490.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.7.49. 295234.
Family and domain databases
InterPro	IPR000477. Reverse_transcriptase. IPR015706. Reverse_transcriptase-related. [Graphical view]
PANTHER	PTHR19446. Rev_txptase. 1 hit.
Pfam	PF00078. RVT_1. 1 hit. [Graphical view]
PROSITE	PS50878. RT_POL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PO22_POPJA

Accession

Primary (citable) accession number: Q03274

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	September 22, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)