Reviewed, UniProtKB/Swiss-Prot Q03266 (PABC_YEAST)

Names and origin

Protein names

Recommended name:
    Aminodeoxychorismate lyase
    EC=4.1.3.38
Alternative name(s):
    4-amino-4-deoxychorismate lyase
      Short name=ADC lyase
      Short name=ADCL

Gene names

Name:	ABZ2
Ordered Locus Names:	YMR289W
ORF Names:	YM8021.15

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts 4-amino-4-deoxychorismate into 4-aminobenzoate (PABA) and pyruvate By similarity.
Catalytic activity	4-amino-4-deoxychorismate = 4-aminobenzoate + pyruvate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; 4-aminobenzoate from chorismate: step 2/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 768 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Folate biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid biosynthetic process Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Ref.2 Inferred from direct assay. Source: SGD
Molecular function	4-amino-4-deoxychorismate lyase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Aminodeoxychorismate lyase		PRO_0000203350

Sequences

	Sequence		Length	Mass (Da)
	Q03266-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 560B9757F6BBBB73	FASTA	374	42,640
	10 20 30 40 50 60 MSLMDNWKTD MESYDEGGLV ANPNFEVLAT FRYDPGFARQ SASKKEIFET PDPRLGLRDE 70 80 90 100 110 120 DIRQQIINED YSSYLRVREV NSGGDLLENI QHPDAWKHDC KTIVCQRVED MLQVIYERFF 130 140 150 160 170 180 LLDEQYQRIR IALSYFKIDF STSLNDLLKL LVENLINCKE GNSEYHEKIQ KMINERQCYK 190 200 210 220 230 240 MRVLVSKTGD IRIEAIPMPM EPILKLTTDY DSVSTYFIKT MLNGFLIDST INWDVVVSSE 250 260 270 280 290 300 PLNASAFTSF KTTSRDHYAR ARVRMQTAIN NLRGSEPTSS VSQCEILFSN KSGLLMEGSI 310 320 330 340 350 360 TNVAVIQKDP NGSKKYVTPR LATGCLCGTM RHYLLRLGLI EEGDIDIGSL TVGNEVLLFN 370 GVMGCIKGTV KTKY

References

[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49704 Genomic DNA. Translation: CAA89787.1. X80836 Genomic DNA. Translation: CAA56798.1.
PIR	S54596.
RefSeq	NP_014016.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q03266. 4 interactions.
STRING	Q03266.
Genome annotation databases
Ensembl	YMR289W; YMR289W; YMR289W; Saccharomyces cerevisiae. [Genome view]
GeneID	855333.
KEGG	sce:YMR289W.
NMPDR	fig|4932.3.peg.5074.
Organism-specific databases
CYGD	YMR289w.
SGD	S000004902. ABZ2.
Phylogenomic databases
HOGENOM	Q03266.
OMA	TIRYDPH
OrthoDB	EOG9M937Z
Enzyme and pathway databases
BRENDA	4.1.3.38. 250.
Gene expression databases
ArrayExpress	Q03266.
Genevestigator	Q03266.
GermOnline	YMR289W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001544. Aminotrans_IV. [Graphical view]
PANTHER	PTHR11825. Aminotrans_IV. 1 hit.
PROSITE	PS00770. AA_TRANSFER_CLASS_4. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979061.

Entry information

Entry name

PABC_YEAST

Accession

Primary (citable) accession number: Q03266
Secondary accession number(s): Q03531

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)