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Protein

RNA exonuclease NGL2

Gene

NGL2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-rRNA processing. Required for the final stage of 3'-end maturation of 5.8S rRNA at site E.1 Publication

GO - Molecular functioni

  • endoribonuclease activity Source: SGD
  • exonuclease activity Source: UniProtKB-KW
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  • rRNA processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32955-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA exonuclease NGL2 (EC:3.1.-.-)
Alternative name(s):
Carbon catabolite repressor protein 4 homolog
Gene namesi
Name:NGL2
Ordered Locus Names:YMR285C
ORF Names:YM8021.11C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYMR285c.
EuPathDBiFungiDB:YMR285C.
SGDiS000004898. NGL2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • intracellular Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 515515RNA exonuclease NGL2PRO_0000218579Add
BLAST

Proteomic databases

MaxQBiQ03264.
PaxDbiQ03264.
PeptideAtlasiQ03264.

Interactioni

Protein-protein interaction databases

BioGridi35465. 56 interactions.
DIPiDIP-894N.
MINTiMINT-4498988.
STRINGi4932.YMR285C.

Structurei

3D structure databases

ProteinModelPortaliQ03264.
SMRiQ03264. Positions 96-318.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CCR4/nocturin family.Curated

Phylogenomic databases

eggNOGiCOG5239.
GeneTreeiENSGT00550000076051.
HOGENOMiHOG000248147.
InParanoidiQ03264.
KOiK14812.
OMAiYPSDHLC.
OrthoDBiEOG73NGD0.

Family and domain databases

Gene3Di3.60.10.10. 2 hits.
InterProiIPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 3 hits.

Sequencei

Sequence statusi: Complete.

Q03264-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQDKEVKVV APDVAPDQEV EINKSVKDAK HQTNDDSLLQ HKKKGKKGKK
60 70 80 90 100
SKPIVTPEHI AKVRAEREVM RKAKRDAMLA QGVDPDCPPE LHFIRRPFLS
110 120 130 140 150
LHEAEPVTGF RFKLMTYNCL AQALIRRKLF PDSGDALKWY RRSKVLLNEF
160 170 180 190 200
KYYNSDVICL QEIDHIQFQS FWKDEFSKLG YDGQYYRNAT KNHGVAIMWR
210 220 230 240 250
RELFHQVDKM LIDYDKESSE SISTRTTTNN VGLVLALKFS EKVLSNLGKK
260 270 280 290 300
SSKKCGILIG TTHLFWHPFG TYERTRQCYI VLKKMKEFMH RVNVLQNEND
310 320 330 340 350
GDLSHWFPFF CGDFNSQPFD TPYLSMTSKP VHYRNRAKTV IGCSTSYKFS
360 370 380 390 400
KVRDGEEGAD DEEGGNIEKY GKDQPESPVP EKFHANEEQS ELVDKMAQLH
410 420 430 440 450
NSLDMRAISL YSVGYKNVHP ENAGLDNDRG EPEISNWANT WRGLLDYLFY
460 470 480 490 500
VKKWDPQSNC QEVETLGDFE KENKVKCRGF LRMPPGNEMT KHGQPHVGEY
510
ASDHLSMVCD LELQL
Length:515
Mass (Da):59,540
Last modified:November 1, 1997 - v1
Checksum:i89B30A7D24D6EBC8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49704 Genomic DNA. Translation: CAA89783.1.
BK006946 Genomic DNA. Translation: DAA10186.1.
PIRiS54592.
RefSeqiNP_014012.1. NM_001182792.1.

Genome annotation databases

EnsemblFungiiYMR285C; YMR285C; YMR285C.
GeneIDi855329.
KEGGisce:YMR285C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49704 Genomic DNA. Translation: CAA89783.1.
BK006946 Genomic DNA. Translation: DAA10186.1.
PIRiS54592.
RefSeqiNP_014012.1. NM_001182792.1.

3D structure databases

ProteinModelPortaliQ03264.
SMRiQ03264. Positions 96-318.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35465. 56 interactions.
DIPiDIP-894N.
MINTiMINT-4498988.
STRINGi4932.YMR285C.

Proteomic databases

MaxQBiQ03264.
PaxDbiQ03264.
PeptideAtlasiQ03264.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR285C; YMR285C; YMR285C.
GeneIDi855329.
KEGGisce:YMR285C.

Organism-specific databases

CYGDiYMR285c.
EuPathDBiFungiDB:YMR285C.
SGDiS000004898. NGL2.

Phylogenomic databases

eggNOGiCOG5239.
GeneTreeiENSGT00550000076051.
HOGENOMiHOG000248147.
InParanoidiQ03264.
KOiK14812.
OMAiYPSDHLC.
OrthoDBiEOG73NGD0.

Enzyme and pathway databases

BioCyciYEAST:G3O-32955-MONOMER.

Miscellaneous databases

NextBioi979049.
PROiQ03264.

Family and domain databases

Gene3Di3.60.10.10. 2 hits.
InterProiIPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 3 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Ngl2p is a Ccr4p-like RNA nuclease essential for the final step in 3'-end processing of 5.8S rRNA in Saccharomyces cerevisiae."
    Faber A.W., van Dijk M., Raue H.A., Vos J.C.
    RNA 8:1095-1101(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNGL2_YEAST
AccessioniPrimary (citable) accession number: Q03264
Secondary accession number(s): D6W0B2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 24, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3570 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.