Q03264 (NGL2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA exonuclease NGL2
EC=3.1.-.-

Alternative name(s):
Carbon catabolite repressor protein 4 homolog

Gene names

Name:	NGL2
Ordered Locus Names:	YMR285C
ORF Names:	YM8021.11C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pre-rRNA processing. Required for the final stage of 3'-end maturation of 5.8S rRNA at site E. Ref.3
Subcellular location	Cytoplasm Potential. Nucleus Potential.
Miscellaneous	Present with 3570 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CCR4/nocturin family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	RNA-binding
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	rRNA processing Inferred from mutant phenotype Ref.3. Source: SGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell intracellular Inferred from direct assay Ref.3. Source: SGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW endoribonuclease activity Inferred from mutant phenotype Ref.3. Source: SGD exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 515	515	RNA exonuclease NGL2		PRO_0000218579

Sequences

	Sequence		Length	Mass (Da)
	Q03264 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 89B30A7D24D6EBC8	FASTA	515	59,540
	10 20 30 40 50 60 MTQDKEVKVV APDVAPDQEV EINKSVKDAK HQTNDDSLLQ HKKKGKKGKK SKPIVTPEHI 70 80 90 100 110 120 AKVRAEREVM RKAKRDAMLA QGVDPDCPPE LHFIRRPFLS LHEAEPVTGF RFKLMTYNCL 130 140 150 160 170 180 AQALIRRKLF PDSGDALKWY RRSKVLLNEF KYYNSDVICL QEIDHIQFQS FWKDEFSKLG 190 200 210 220 230 240 YDGQYYRNAT KNHGVAIMWR RELFHQVDKM LIDYDKESSE SISTRTTTNN VGLVLALKFS 250 260 270 280 290 300 EKVLSNLGKK SSKKCGILIG TTHLFWHPFG TYERTRQCYI VLKKMKEFMH RVNVLQNEND 310 320 330 340 350 360 GDLSHWFPFF CGDFNSQPFD TPYLSMTSKP VHYRNRAKTV IGCSTSYKFS KVRDGEEGAD 370 380 390 400 410 420 DEEGGNIEKY GKDQPESPVP EKFHANEEQS ELVDKMAQLH NSLDMRAISL YSVGYKNVHP 430 440 450 460 470 480 ENAGLDNDRG EPEISNWANT WRGLLDYLFY VKKWDPQSNC QEVETLGDFE KENKVKCRGF 490 500 510 LRMPPGNEMT KHGQPHVGEY ASDHLSMVCD LELQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Ngl2p is a Ccr4p-like RNA nuclease essential for the final step in 3'-end processing of 5.8S rRNA in Saccharomyces cerevisiae." Faber A.W., van Dijk M., Raue H.A., Vos J.C. RNA 8:1095-1101(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49704 Genomic DNA. Translation: CAA89783.1. BK006946 Genomic DNA. Translation: DAA10186.1.
PIR	S54592.
RefSeq	NP_014012.1. NM_001182792.1.
3D structure databases
ProteinModelPortal	Q03264.
SMR	Q03264. Positions 96-318.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-894N.
IntAct	Q03264. 1 interaction.
STRING	4932.YMR285C.
Proteomic databases
PaxDb	Q03264.
PeptideAtlas	Q03264.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR285C; YMR285C; YMR285C.
GeneID	855329.
KEGG	sce:YMR285C.
Organism-specific databases
CYGD	YMR285c.
SGD	S000004898. NGL2.
Phylogenomic databases
eggNOG	COG5239.
GeneTree	ENSGT00550000076051.
HOGENOM	HOG000248147.
KO	K14812.
OMA	MRAISLY.
OrthoDB	EOG4K3Q4X.
Gene expression databases
Genevestigator	Q03264.
GermOnline	YMR285C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005135. Endo/exonuclease/phosphatase. [Graphical view]
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
SUPFAM	SSF56219. Exo_endo_phos. 1 hit.
ProtoNet	Search...
Other
NextBio	979049.

Entry information

Entry name

NGL2_YEAST

Accession

Primary (citable) accession number: Q03264
Secondary accession number(s): D6W0B2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program