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Q03262

- PGM3_YEAST

UniProt

Q03262 - PGM3_YEAST

Protein

Phosphoglucomutase-3

Gene

PGM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    This enzyme participates in both the breakdown and synthesis of glucose.1 Publication

    Catalytic activityi

    Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Kineticsi

    1. KM=2.0 mM for alpha-D-glucose 1-phosphate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei158 – 1581Phosphoserine intermediate
    Metal bindingi158 – 1581Magnesium; via phosphate groupBy similarity
    Metal bindingi325 – 3251MagnesiumBy similarity
    Metal bindingi327 – 3271MagnesiumBy similarity
    Metal bindingi329 – 3291MagnesiumBy similarity

    GO - Molecular functioni

    1. magnesium ion binding Source: InterPro
    2. phosphoglucomutase activity Source: UniProtKB-EC
    3. phosphopentomutase activity Source: SGD

    GO - Biological processi

    1. glucose metabolic process Source: UniProtKB-KW
    2. guanosine catabolic process Source: SGD
    3. inosine catabolic process Source: SGD
    4. purine ribonucleoside salvage Source: SGD

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32949-MONOMER.
    ReactomeiREACT_191292. Glycogen synthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoglucomutase-3 (EC:5.4.2.2)
    Short name:
    PGM-3
    Alternative name(s):
    Glucose phosphomutase-3
    Gene namesi
    Name:PGM3
    Ordered Locus Names:YMR278W
    ORF Names:YM8021.04
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR278w.
    SGDiS000004891. PGM3.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi158 – 1581S → T: Loss of function. 1 Publication
    Mutagenesisi326 – 3261P → G: No effect. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 622622Phosphoglucomutase-3PRO_0000148022Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei158 – 1581Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ03262.
    PaxDbiQ03262.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03262.

    Interactioni

    Protein-protein interaction databases

    BioGridi35457. 4 interactions.
    DIPiDIP-5066N.
    MINTiMINT-519611.
    STRINGi4932.YMR278W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03262.
    SMRiQ03262. Positions 55-333.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the phosphohexose mutase family.Curated

    Phylogenomic databases

    eggNOGiCOG1109.
    GeneTreeiENSGT00390000017247.
    HOGENOMiHOG000268676.
    KOiK01835.
    OMAiHINAVED.
    OrthoDBiEOG7ZGXC9.

    Family and domain databases

    Gene3Di3.30.310.50. 1 hit.
    3.40.120.10. 3 hits.
    InterProiIPR005844. A-D-PHexomutase_a/b/a-I.
    IPR016055. A-D-PHexomutase_a/b/a-I/II/III.
    IPR005845. A-D-PHexomutase_a/b/a-II.
    IPR005846. A-D-PHexomutase_a/b/a-III.
    IPR005843. A-D-PHexomutase_C.
    IPR016066. A-D-PHexomutase_CS.
    [Graphical view]
    PfamiPF02878. PGM_PMM_I. 1 hit.
    PF02879. PGM_PMM_II. 1 hit.
    PF02880. PGM_PMM_III. 1 hit.
    PF00408. PGM_PMM_IV. 1 hit.
    [Graphical view]
    SUPFAMiSSF53738. SSF53738. 3 hits.
    SSF55957. SSF55957. 1 hit.
    PROSITEiPS00710. PGM_PMM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03262-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLQGILETVP SDLKDPISLW FKQDRNPKTI EEVTALCKKS DWNELHKRFD    50
    SRIQFGTAGL RSQMQAGFSR MNTLVVIQAS QGLATYVRQQ FPDNLVAVVG 100
    HDHRFHSKEF ARATAAAFLL KGFKVHYLNP DHEFVHTPLV PFAVDKLKAS 150
    VGVMITASHN PKMDNGYKVY YSNGCQIIPP HDHAISDSID ANLEPWANVW 200
    DFDDVLNKAL KQGKLMYSRE EMLKLYLEEV SKNLVEINPL KLEVKAKPWF 250
    VYTPMHGVGF DIFSTIVKKT LCLVEGKDYL CVPEQQNPDP SFPTVGFPNP 300
    EEKGALDIGI NLAEKHDIDL LVANDPDADR FSVAVKDMQS GEWRQLTGNE 350
    IGFLFAFYEY QKYKSMDKEF QHVHPLAMLN STVSSQMIKK MAEIEGFHYE 400
    DTLTGFKWIG NRAILLEKKG YYVPFGFEEA IGYMFPAMEH DKDGISASIV 450
    FLQAYCKWKI DHNLDPLNVL ENGFKKYGVF KEYNGYYVVP NPTVTKDIFD 500
    YIRNVYTPEG ASYPSSIGEE IEVLYYRDLT TGYQSDTINH KPTLPVDPTS 550
    QMITVSARPS NGSENEHIRF TIRGSGTEPK LKVYIEACAN EEQRASFLAK 600
    LTWNVLRREW FRPDEMNIVT KF 622
    Length:622
    Mass (Da):71,069
    Last modified:November 1, 1997 - v1
    Checksum:iE402A69551B36CD3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49704 Genomic DNA. Translation: CAA89776.1.
    BK006946 Genomic DNA. Translation: DAA10179.1.
    PIRiS54585.
    RefSeqiNP_014005.1. NM_001182785.1.

    Genome annotation databases

    EnsemblFungiiYMR278W; YMR278W; YMR278W.
    GeneIDi855321.
    KEGGisce:YMR278W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49704 Genomic DNA. Translation: CAA89776.1 .
    BK006946 Genomic DNA. Translation: DAA10179.1 .
    PIRi S54585.
    RefSeqi NP_014005.1. NM_001182785.1.

    3D structure databases

    ProteinModelPortali Q03262.
    SMRi Q03262. Positions 55-333.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35457. 4 interactions.
    DIPi DIP-5066N.
    MINTi MINT-519611.
    STRINGi 4932.YMR278W.

    Proteomic databases

    MaxQBi Q03262.
    PaxDbi Q03262.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR278W ; YMR278W ; YMR278W .
    GeneIDi 855321.
    KEGGi sce:YMR278W.

    Organism-specific databases

    CYGDi YMR278w.
    SGDi S000004891. PGM3.

    Phylogenomic databases

    eggNOGi COG1109.
    GeneTreei ENSGT00390000017247.
    HOGENOMi HOG000268676.
    KOi K01835.
    OMAi HINAVED.
    OrthoDBi EOG7ZGXC9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32949-MONOMER.
    Reactomei REACT_191292. Glycogen synthesis.

    Miscellaneous databases

    NextBioi 979026.

    Gene expression databases

    Genevestigatori Q03262.

    Family and domain databases

    Gene3Di 3.30.310.50. 1 hit.
    3.40.120.10. 3 hits.
    InterProi IPR005844. A-D-PHexomutase_a/b/a-I.
    IPR016055. A-D-PHexomutase_a/b/a-I/II/III.
    IPR005845. A-D-PHexomutase_a/b/a-II.
    IPR005846. A-D-PHexomutase_a/b/a-III.
    IPR005843. A-D-PHexomutase_C.
    IPR016066. A-D-PHexomutase_CS.
    [Graphical view ]
    Pfami PF02878. PGM_PMM_I. 1 hit.
    PF02879. PGM_PMM_II. 1 hit.
    PF02880. PGM_PMM_III. 1 hit.
    PF00408. PGM_PMM_IV. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53738. SSF53738. 3 hits.
    SSF55957. SSF55957. 1 hit.
    PROSITEi PS00710. PGM_PMM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-158, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "Molecular characterization reveals that YMR278w encoded protein is environmental stress response homologue of Saccharomyces cerevisiae PGM2."
      Tiwari A., Bhat J.P.
      Biochem. Biophys. Res. Commun. 366:340-345(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF SER-158 AND PRO-326.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPGM3_YEAST
    AccessioniPrimary (citable) accession number: Q03262
    Secondary accession number(s): D6W0A5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4960 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3