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Q03262

- PGM3_YEAST

UniProt

Q03262 - PGM3_YEAST

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Protein
Phosphoglucomutase-3
Gene
PGM3, YMR278W, YM8021.04
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This enzyme participates in both the breakdown and synthesis of glucose.1 Publication

Catalytic activityi

Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate.

Cofactori

Binds 1 magnesium ion per subunit By similarity.

Kineticsi

  1. KM=2.0 mM for alpha-D-glucose 1-phosphate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei158 – 1581Phosphoserine intermediate
Metal bindingi158 – 1581Magnesium; via phosphate group By similarity
Metal bindingi325 – 3251Magnesium By similarity
Metal bindingi327 – 3271Magnesium By similarity
Metal bindingi329 – 3291Magnesium By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. phosphoglucomutase activity Source: UniProtKB-EC
  3. phosphopentomutase activity Source: SGD

GO - Biological processi

  1. glucose metabolic process Source: UniProtKB-KW
  2. guanosine catabolic process Source: SGD
  3. inosine catabolic process Source: SGD
  4. purine ribonucleoside salvage Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32949-MONOMER.
ReactomeiREACT_191292. Glycogen synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglucomutase-3 (EC:5.4.2.2)
Short name:
PGM-3
Alternative name(s):
Glucose phosphomutase-3
Gene namesi
Name:PGM3
Ordered Locus Names:YMR278W
ORF Names:YM8021.04
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR278w.
SGDiS000004891. PGM3.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi158 – 1581S → T: Loss of function. 1 Publication
Mutagenesisi326 – 3261P → G: No effect. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Phosphoglucomutase-3
PRO_0000148022Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei158 – 1581Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03262.
PaxDbiQ03262.

Expressioni

Gene expression databases

GenevestigatoriQ03262.

Interactioni

Protein-protein interaction databases

BioGridi35457. 4 interactions.
DIPiDIP-5066N.
MINTiMINT-519611.
STRINGi4932.YMR278W.

Structurei

3D structure databases

ProteinModelPortaliQ03262.
SMRiQ03262. Positions 55-333.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1109.
GeneTreeiENSGT00390000017247.
HOGENOMiHOG000268676.
KOiK01835.
OMAiHINAVED.
OrthoDBiEOG7ZGXC9.

Family and domain databases

Gene3Di3.30.310.50. 1 hit.
3.40.120.10. 3 hits.
InterProiIPR005844. A-D-PHexomutase_a/b/a-I.
IPR016055. A-D-PHexomutase_a/b/a-I/II/III.
IPR005845. A-D-PHexomutase_a/b/a-II.
IPR005846. A-D-PHexomutase_a/b/a-III.
IPR005843. A-D-PHexomutase_C.
IPR016066. A-D-PHexomutase_CS.
[Graphical view]
PfamiPF02878. PGM_PMM_I. 1 hit.
PF02879. PGM_PMM_II. 1 hit.
PF02880. PGM_PMM_III. 1 hit.
PF00408. PGM_PMM_IV. 1 hit.
[Graphical view]
SUPFAMiSSF53738. SSF53738. 3 hits.
SSF55957. SSF55957. 1 hit.
PROSITEiPS00710. PGM_PMM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03262-1 [UniParc]FASTAAdd to Basket

« Hide

MLQGILETVP SDLKDPISLW FKQDRNPKTI EEVTALCKKS DWNELHKRFD    50
SRIQFGTAGL RSQMQAGFSR MNTLVVIQAS QGLATYVRQQ FPDNLVAVVG 100
HDHRFHSKEF ARATAAAFLL KGFKVHYLNP DHEFVHTPLV PFAVDKLKAS 150
VGVMITASHN PKMDNGYKVY YSNGCQIIPP HDHAISDSID ANLEPWANVW 200
DFDDVLNKAL KQGKLMYSRE EMLKLYLEEV SKNLVEINPL KLEVKAKPWF 250
VYTPMHGVGF DIFSTIVKKT LCLVEGKDYL CVPEQQNPDP SFPTVGFPNP 300
EEKGALDIGI NLAEKHDIDL LVANDPDADR FSVAVKDMQS GEWRQLTGNE 350
IGFLFAFYEY QKYKSMDKEF QHVHPLAMLN STVSSQMIKK MAEIEGFHYE 400
DTLTGFKWIG NRAILLEKKG YYVPFGFEEA IGYMFPAMEH DKDGISASIV 450
FLQAYCKWKI DHNLDPLNVL ENGFKKYGVF KEYNGYYVVP NPTVTKDIFD 500
YIRNVYTPEG ASYPSSIGEE IEVLYYRDLT TGYQSDTINH KPTLPVDPTS 550
QMITVSARPS NGSENEHIRF TIRGSGTEPK LKVYIEACAN EEQRASFLAK 600
LTWNVLRREW FRPDEMNIVT KF 622
Length:622
Mass (Da):71,069
Last modified:November 1, 1997 - v1
Checksum:iE402A69551B36CD3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49704 Genomic DNA. Translation: CAA89776.1.
BK006946 Genomic DNA. Translation: DAA10179.1.
PIRiS54585.
RefSeqiNP_014005.1. NM_001182785.1.

Genome annotation databases

EnsemblFungiiYMR278W; YMR278W; YMR278W.
GeneIDi855321.
KEGGisce:YMR278W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49704 Genomic DNA. Translation: CAA89776.1 .
BK006946 Genomic DNA. Translation: DAA10179.1 .
PIRi S54585.
RefSeqi NP_014005.1. NM_001182785.1.

3D structure databases

ProteinModelPortali Q03262.
SMRi Q03262. Positions 55-333.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35457. 4 interactions.
DIPi DIP-5066N.
MINTi MINT-519611.
STRINGi 4932.YMR278W.

Proteomic databases

MaxQBi Q03262.
PaxDbi Q03262.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR278W ; YMR278W ; YMR278W .
GeneIDi 855321.
KEGGi sce:YMR278W.

Organism-specific databases

CYGDi YMR278w.
SGDi S000004891. PGM3.

Phylogenomic databases

eggNOGi COG1109.
GeneTreei ENSGT00390000017247.
HOGENOMi HOG000268676.
KOi K01835.
OMAi HINAVED.
OrthoDBi EOG7ZGXC9.

Enzyme and pathway databases

BioCyci YEAST:G3O-32949-MONOMER.
Reactomei REACT_191292. Glycogen synthesis.

Miscellaneous databases

NextBioi 979026.

Gene expression databases

Genevestigatori Q03262.

Family and domain databases

Gene3Di 3.30.310.50. 1 hit.
3.40.120.10. 3 hits.
InterProi IPR005844. A-D-PHexomutase_a/b/a-I.
IPR016055. A-D-PHexomutase_a/b/a-I/II/III.
IPR005845. A-D-PHexomutase_a/b/a-II.
IPR005846. A-D-PHexomutase_a/b/a-III.
IPR005843. A-D-PHexomutase_C.
IPR016066. A-D-PHexomutase_CS.
[Graphical view ]
Pfami PF02878. PGM_PMM_I. 1 hit.
PF02879. PGM_PMM_II. 1 hit.
PF02880. PGM_PMM_III. 1 hit.
PF00408. PGM_PMM_IV. 1 hit.
[Graphical view ]
SUPFAMi SSF53738. SSF53738. 3 hits.
SSF55957. SSF55957. 1 hit.
PROSITEi PS00710. PGM_PMM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-158, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Molecular characterization reveals that YMR278w encoded protein is environmental stress response homologue of Saccharomyces cerevisiae PGM2."
    Tiwari A., Bhat J.P.
    Biochem. Biophys. Res. Commun. 366:340-345(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF SER-158 AND PRO-326.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPGM3_YEAST
AccessioniPrimary (citable) accession number: Q03262
Secondary accession number(s): D6W0A5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4960 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi