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Q03262 (PGM3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglucomutase-3

Short name=PGM-3
EC=5.4.2.2
Alternative name(s):
Glucose phosphomutase-3
Gene names
Name:PGM3
Ordered Locus Names:YMR278W
ORF Names:YM8021.04
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length622 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme participates in both the breakdown and synthesis of glucose. Ref.7

Catalytic activity

Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subcellular location

Cytoplasm. Nucleus Ref.3.

Miscellaneous

Present with 4960 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the phosphohexose mutase family.

Biophysicochemical properties

Kinetic parameters:

KM=2.0 mM for alpha-D-glucose 1-phosphate Ref.7

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 622622Phosphoglucomutase-3
PRO_0000148022

Sites

Active site1581Phosphoserine intermediate
Metal binding1581Magnesium; via phosphate group By similarity
Metal binding3251Magnesium By similarity
Metal binding3271Magnesium By similarity
Metal binding3291Magnesium By similarity

Amino acid modifications

Modified residue1581Phosphoserine Ref.5

Experimental info

Mutagenesis1581S → T: Loss of function. Ref.7
Mutagenesis3261P → G: No effect. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q03262 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: E402A69551B36CD3

FASTA62271,069
        10         20         30         40         50         60 
MLQGILETVP SDLKDPISLW FKQDRNPKTI EEVTALCKKS DWNELHKRFD SRIQFGTAGL 

        70         80         90        100        110        120 
RSQMQAGFSR MNTLVVIQAS QGLATYVRQQ FPDNLVAVVG HDHRFHSKEF ARATAAAFLL 

       130        140        150        160        170        180 
KGFKVHYLNP DHEFVHTPLV PFAVDKLKAS VGVMITASHN PKMDNGYKVY YSNGCQIIPP 

       190        200        210        220        230        240 
HDHAISDSID ANLEPWANVW DFDDVLNKAL KQGKLMYSRE EMLKLYLEEV SKNLVEINPL 

       250        260        270        280        290        300 
KLEVKAKPWF VYTPMHGVGF DIFSTIVKKT LCLVEGKDYL CVPEQQNPDP SFPTVGFPNP 

       310        320        330        340        350        360 
EEKGALDIGI NLAEKHDIDL LVANDPDADR FSVAVKDMQS GEWRQLTGNE IGFLFAFYEY 

       370        380        390        400        410        420 
QKYKSMDKEF QHVHPLAMLN STVSSQMIKK MAEIEGFHYE DTLTGFKWIG NRAILLEKKG 

       430        440        450        460        470        480 
YYVPFGFEEA IGYMFPAMEH DKDGISASIV FLQAYCKWKI DHNLDPLNVL ENGFKKYGVF 

       490        500        510        520        530        540 
KEYNGYYVVP NPTVTKDIFD YIRNVYTPEG ASYPSSIGEE IEVLYYRDLT TGYQSDTINH 

       550        560        570        580        590        600 
KPTLPVDPTS QMITVSARPS NGSENEHIRF TIRGSGTEPK LKVYIEACAN EEQRASFLAK 

       610        620 
LTWNVLRREW FRPDEMNIVT KF 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-158, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"Molecular characterization reveals that YMR278w encoded protein is environmental stress response homologue of Saccharomyces cerevisiae PGM2."
Tiwari A., Bhat J.P.
Biochem. Biophys. Res. Commun. 366:340-345(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF SER-158 AND PRO-326.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49704 Genomic DNA. Translation: CAA89776.1.
BK006946 Genomic DNA. Translation: DAA10179.1.
PIRS54585.
RefSeqNP_014005.1. NM_001182785.1.

3D structure databases

ProteinModelPortalQ03262.
SMRQ03262. Positions 55-333.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35457. 4 interactions.
DIPDIP-5066N.
MINTMINT-519611.
STRING4932.YMR278W.

Proteomic databases

MaxQBQ03262.
PaxDbQ03262.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR278W; YMR278W; YMR278W.
GeneID855321.
KEGGsce:YMR278W.

Organism-specific databases

CYGDYMR278w.
SGDS000004891. PGM3.

Phylogenomic databases

eggNOGCOG1109.
GeneTreeENSGT00390000017247.
HOGENOMHOG000268676.
KOK01835.
OMAHINAVED.
OrthoDBEOG7ZGXC9.

Enzyme and pathway databases

BioCycYEAST:G3O-32949-MONOMER.

Gene expression databases

GenevestigatorQ03262.

Family and domain databases

Gene3D3.30.310.50. 1 hit.
3.40.120.10. 3 hits.
InterProIPR005844. A-D-PHexomutase_a/b/a-I.
IPR016055. A-D-PHexomutase_a/b/a-I/II/III.
IPR005845. A-D-PHexomutase_a/b/a-II.
IPR005846. A-D-PHexomutase_a/b/a-III.
IPR005843. A-D-PHexomutase_C.
IPR016066. A-D-PHexomutase_CS.
[Graphical view]
PfamPF02878. PGM_PMM_I. 1 hit.
PF02879. PGM_PMM_II. 1 hit.
PF02880. PGM_PMM_III. 1 hit.
PF00408. PGM_PMM_IV. 1 hit.
[Graphical view]
SUPFAMSSF53738. SSF53738. 3 hits.
SSF55957. SSF55957. 1 hit.
PROSITEPS00710. PGM_PMM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979026.

Entry information

Entry namePGM3_YEAST
AccessionPrimary (citable) accession number: Q03262
Secondary accession number(s): D6W0A5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families