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Q03254

- FCP1_YEAST

UniProt

Q03254 - FCP1_YEAST

Protein

RNA polymerase II subunit A C-terminal domain phosphatase

Gene

FCP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II.1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    GO - Molecular functioni

    1. protein serine/threonine phosphatase activity Source: SGD

    GO - Biological processi

    1. dephosphorylation of RNA polymerase II C-terminal domain Source: SGD
    2. regulation of transcription from RNA polymerase II promoter Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32948-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA polymerase II subunit A C-terminal domain phosphatase (EC:3.1.3.16)
    Alternative name(s):
    CTD phosphatase FCP1
    Gene namesi
    Name:FCP1
    Ordered Locus Names:YMR277W
    ORF Names:YM8021.03
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR277w.
    SGDiS000004890. FCP1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 732732RNA polymerase II subunit A C-terminal domain phosphatasePRO_0000212568Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei701 – 7011Phosphoserine1 Publication
    Modified residuei718 – 7181Phosphoserine1 Publication
    Modified residuei720 – 7201Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ03254.
    PaxDbiQ03254.
    PeptideAtlasiQ03254.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03254.

    Interactioni

    Protein-protein interaction databases

    BioGridi35456. 66 interactions.
    DIPiDIP-2330N.
    IntActiQ03254. 11 interactions.
    MINTiMINT-501044.
    STRINGi4932.YMR277W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03254.
    SMRiQ03254. Positions 160-594.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini170 – 363194FCP1 homologyPROSITE-ProRule annotationAdd
    BLAST
    Domaini499 – 59395BRCTPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 BRCT domain.PROSITE-ProRule annotation
    Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5190.
    GeneTreeiENSGT00390000015641.
    HOGENOMiHOG000197682.
    KOiK15732.
    OMAiPDKKLFG.
    OrthoDBiEOG7TXKSN.

    Family and domain databases

    Gene3Di3.40.50.1000. 3 hits.
    3.40.50.10190. 1 hit.
    InterProiIPR001357. BRCT_dom.
    IPR011947. FCP1_euk.
    IPR023214. HAD-like_dom.
    IPR004274. NIF.
    [Graphical view]
    PfamiPF00533. BRCT. 1 hit.
    PF03031. NIF. 1 hit.
    [Graphical view]
    SMARTiSM00292. BRCT. 1 hit.
    SM00577. CPDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52113. SSF52113. 1 hit.
    SSF56784. SSF56784. 2 hits.
    TIGRFAMsiTIGR02250. FCP1_euk. 1 hit.
    PROSITEiPS50172. BRCT. 1 hit.
    PS50969. FCP1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03254-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTQIRSPQG LPYPIQIDKL IPSVGSYLHE GDRLLVYKFW YLVERASDTG    50
    DDDNEHDVSP GGSAGSNGVS PPTKQLRESI EFFESPYEGD LISWNVDVGD 100
    EVATANQVIC EIKRPCNHDI VYGGLCTQCG KEVSADAFDG VPLDVVGDVD 150
    LQISETEAIR TGKALKEHLR RDKKLILVVD LDQTIIHCGV DPTIAEWKND 200
    PNNPNFETLR DVKSFTLDEE LVLPLMYMND DGSMLRPPPV RKCWYYVKVR 250
    PGLKEFFAKV APLFEMHIYT MATRAYALQI AKIVDPTGEL FGDRILSRDE 300
    NGSLTTKSLA KLFPTDQSMV VVIDDRGDVW NWCPNLIKVV PYNFFVGVGD 350
    INSNFLPKQS TGMLQLGRKT RQKSQESQEL LTDIMDNEKK LQEKIDKEVK 400
    RQEEKLNHQL ATAEEPPANE SKEELTKKLE YSASLEVQQQ NRPLAKLQKH 450
    LHDQKLLVDD DDELYYLMGT LSNIHKTYYD MLSQQNEPEP NLMEIIPSLK 500
    QKVFQNCYFV FSGLIPLGTD IQRSDIVIWT STFGATSTPD IDYLTTHLIT 550
    KNPSTYKARL AKKFNPQIKI VHPDWIFECL VNWKKVDEKP YTLIVDSPIS 600
    DEELQNFQTQ LQKRQEYLEE TQEQQHMLTS QENLNLFAAG TSWLNNDDDE 650
    DIPDTASDDD EDDDHDDESD DENNSEGIDR KRSIEDNHDD TSQKKTKAEP 700
    SQDGPVQHKG EGDDNEDSDS QLEEELMDML DD 732
    Length:732
    Mass (Da):83,441
    Last modified:November 1, 1997 - v1
    Checksum:iEACA2A7D33A983C6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49704 Genomic DNA. Translation: CAA89775.1.
    BK006946 Genomic DNA. Translation: DAA10178.1.
    PIRiS54584.
    RefSeqiNP_014004.1. NM_001182784.1.

    Genome annotation databases

    EnsemblFungiiYMR277W; YMR277W; YMR277W.
    GeneIDi855320.
    KEGGisce:YMR277W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49704 Genomic DNA. Translation: CAA89775.1 .
    BK006946 Genomic DNA. Translation: DAA10178.1 .
    PIRi S54584.
    RefSeqi NP_014004.1. NM_001182784.1.

    3D structure databases

    ProteinModelPortali Q03254.
    SMRi Q03254. Positions 160-594.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35456. 66 interactions.
    DIPi DIP-2330N.
    IntActi Q03254. 11 interactions.
    MINTi MINT-501044.
    STRINGi 4932.YMR277W.

    Proteomic databases

    MaxQBi Q03254.
    PaxDbi Q03254.
    PeptideAtlasi Q03254.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR277W ; YMR277W ; YMR277W .
    GeneIDi 855320.
    KEGGi sce:YMR277W.

    Organism-specific databases

    CYGDi YMR277w.
    SGDi S000004890. FCP1.

    Phylogenomic databases

    eggNOGi COG5190.
    GeneTreei ENSGT00390000015641.
    HOGENOMi HOG000197682.
    KOi K15732.
    OMAi PDKKLFG.
    OrthoDBi EOG7TXKSN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32948-MONOMER.

    Miscellaneous databases

    NextBioi 979023.
    PROi Q03254.

    Gene expression databases

    Genevestigatori Q03254.

    Family and domain databases

    Gene3Di 3.40.50.1000. 3 hits.
    3.40.50.10190. 1 hit.
    InterProi IPR001357. BRCT_dom.
    IPR011947. FCP1_euk.
    IPR023214. HAD-like_dom.
    IPR004274. NIF.
    [Graphical view ]
    Pfami PF00533. BRCT. 1 hit.
    PF03031. NIF. 1 hit.
    [Graphical view ]
    SMARTi SM00292. BRCT. 1 hit.
    SM00577. CPDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52113. SSF52113. 1 hit.
    SSF56784. SSF56784. 2 hits.
    TIGRFAMsi TIGR02250. FCP1_euk. 1 hit.
    PROSITEi PS50172. BRCT. 1 hit.
    PS50969. FCP1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Purification and characterization of an RNA polymerase II phosphatase from yeast."
      Chambers R.S., Kane C.M.
      J. Biol. Chem. 271:24498-24504(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    4. "An unusual eukaryotic protein phosphatase required for transcription by RNA polymerase II and CTD dephosphorylation in S. cerevisiae."
      Kobor M.S., Archambault J., Lester W., Holstege F.C.P., Gileadi O., Jansma D.B., Jennings E.G., Kouyoumdjian F., Davidson A.R., Young R.A., Greenblatt J.
      Mol. Cell 4:55-62(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-701, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-718 AND SER-720, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiFCP1_YEAST
    AccessioniPrimary (citable) accession number: Q03254
    Secondary accession number(s): D6W0A4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6550 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3