Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q03254 (FCP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA polymerase II subunit A C-terminal domain phosphatase

EC=3.1.3.16
Alternative name(s):
CTD phosphatase FCP1
Gene names
Name:FCP1
Ordered Locus Names:YMR277W
ORF Names:YM8021.03
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length732 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II. Ref.4

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Subcellular location

Nucleus.

Miscellaneous

Present with 6550 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 BRCT domain.

Contains 1 FCP1 homology domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 732732RNA polymerase II subunit A C-terminal domain phosphatase
PRO_0000212568

Regions

Domain170 – 363194FCP1 homology
Domain499 – 59395BRCT

Amino acid modifications

Modified residue7011Phosphoserine Ref.6
Modified residue7181Phosphoserine Ref.7
Modified residue7201Phosphoserine Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q03254 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: EACA2A7D33A983C6

FASTA73283,441
        10         20         30         40         50         60 
MTTQIRSPQG LPYPIQIDKL IPSVGSYLHE GDRLLVYKFW YLVERASDTG DDDNEHDVSP 

        70         80         90        100        110        120 
GGSAGSNGVS PPTKQLRESI EFFESPYEGD LISWNVDVGD EVATANQVIC EIKRPCNHDI 

       130        140        150        160        170        180 
VYGGLCTQCG KEVSADAFDG VPLDVVGDVD LQISETEAIR TGKALKEHLR RDKKLILVVD 

       190        200        210        220        230        240 
LDQTIIHCGV DPTIAEWKND PNNPNFETLR DVKSFTLDEE LVLPLMYMND DGSMLRPPPV 

       250        260        270        280        290        300 
RKCWYYVKVR PGLKEFFAKV APLFEMHIYT MATRAYALQI AKIVDPTGEL FGDRILSRDE 

       310        320        330        340        350        360 
NGSLTTKSLA KLFPTDQSMV VVIDDRGDVW NWCPNLIKVV PYNFFVGVGD INSNFLPKQS 

       370        380        390        400        410        420 
TGMLQLGRKT RQKSQESQEL LTDIMDNEKK LQEKIDKEVK RQEEKLNHQL ATAEEPPANE 

       430        440        450        460        470        480 
SKEELTKKLE YSASLEVQQQ NRPLAKLQKH LHDQKLLVDD DDELYYLMGT LSNIHKTYYD 

       490        500        510        520        530        540 
MLSQQNEPEP NLMEIIPSLK QKVFQNCYFV FSGLIPLGTD IQRSDIVIWT STFGATSTPD 

       550        560        570        580        590        600 
IDYLTTHLIT KNPSTYKARL AKKFNPQIKI VHPDWIFECL VNWKKVDEKP YTLIVDSPIS 

       610        620        630        640        650        660 
DEELQNFQTQ LQKRQEYLEE TQEQQHMLTS QENLNLFAAG TSWLNNDDDE DIPDTASDDD 

       670        680        690        700        710        720 
EDDDHDDESD DENNSEGIDR KRSIEDNHDD TSQKKTKAEP SQDGPVQHKG EGDDNEDSDS 

       730 
QLEEELMDML DD 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Purification and characterization of an RNA polymerase II phosphatase from yeast."
Chambers R.S., Kane C.M.
J. Biol. Chem. 271:24498-24504(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[4]"An unusual eukaryotic protein phosphatase required for transcription by RNA polymerase II and CTD dephosphorylation in S. cerevisiae."
Kobor M.S., Archambault J., Lester W., Holstege F.C.P., Gileadi O., Jansma D.B., Jennings E.G., Kouyoumdjian F., Davidson A.R., Young R.A., Greenblatt J.
Mol. Cell 4:55-62(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-701, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-718 AND SER-720, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49704 Genomic DNA. Translation: CAA89775.1.
BK006946 Genomic DNA. Translation: DAA10178.1.
PIRS54584.
RefSeqNP_014004.1. NM_001182784.1.

3D structure databases

ProteinModelPortalQ03254.
SMRQ03254. Positions 160-594.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35456. 64 interactions.
DIPDIP-2330N.
IntActQ03254. 11 interactions.
MINTMINT-501044.
STRING4932.YMR277W.

Proteomic databases

PaxDbQ03254.
PeptideAtlasQ03254.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR277W; YMR277W; YMR277W.
GeneID855320.
KEGGsce:YMR277W.

Organism-specific databases

CYGDYMR277w.
SGDS000004890. FCP1.

Phylogenomic databases

eggNOGCOG5190.
GeneTreeENSGT00390000015641.
HOGENOMHOG000197682.
KOK15732.
OMAPDKKLFG.
OrthoDBEOG7TXKSN.

Enzyme and pathway databases

BioCycYEAST:G3O-32948-MONOMER.

Gene expression databases

GenevestigatorQ03254.

Family and domain databases

Gene3D3.40.50.1000. 3 hits.
3.40.50.10190. 1 hit.
InterProIPR001357. BRCT_dom.
IPR011947. FCP1_euk.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamPF00533. BRCT. 1 hit.
PF03031. NIF. 1 hit.
[Graphical view]
SMARTSM00292. BRCT. 1 hit.
SM00577. CPDc. 1 hit.
[Graphical view]
SUPFAMSSF52113. SSF52113. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsTIGR02250. FCP1_euk. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979023.
PROQ03254.

Entry information

Entry nameFCP1_YEAST
AccessionPrimary (citable) accession number: Q03254
Secondary accession number(s): D6W0A4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families