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Protein

RNA polymerase II subunit A C-terminal domain phosphatase

Gene

FCP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II.1 Publication

Miscellaneous

Present with 6550 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

GO - Molecular functioni

GO - Biological processi

  • dephosphorylation of RNA polymerase II C-terminal domain Source: SGD
  • regulation of transcription from RNA polymerase II promoter Source: SGD

Keywordsi

Molecular functionHydrolase, Protein phosphatase

Enzyme and pathway databases

BioCyciYEAST:G3O-32948-MONOMER.
ReactomeiR-SCE-113418. Formation of the Early Elongation Complex.
R-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-6796648. TP53 Regulates Transcription of DNA Repair Genes.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II subunit A C-terminal domain phosphatase (EC:3.1.3.16)
Alternative name(s):
CTD phosphatase FCP1
Gene namesi
Name:FCP1
Ordered Locus Names:YMR277W
ORF Names:YM8021.03
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR277W.
SGDiS000004890. FCP1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • DNA-directed RNA polymerase II, holoenzyme Source: GO_Central
  • nucleus Source: SGD

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002125681 – 732RNA polymerase II subunit A C-terminal domain phosphataseAdd BLAST732

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki74Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei701PhosphoserineCombined sources1
Modified residuei718PhosphoserineCombined sources1
Modified residuei720PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ03254.
PRIDEiQ03254.

PTM databases

iPTMnetiQ03254.

Interactioni

Protein-protein interaction databases

BioGridi35456. 582 interactors.
DIPiDIP-2330N.
IntActiQ03254. 11 interactors.
MINTiMINT-501044.

Structurei

3D structure databases

ProteinModelPortaliQ03254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini170 – 363FCP1 homologyPROSITE-ProRule annotationAdd BLAST194
Domaini499 – 593BRCTPROSITE-ProRule annotationAdd BLAST95

Phylogenomic databases

GeneTreeiENSGT00390000015641.
HOGENOMiHOG000197682.
InParanoidiQ03254.
KOiK15732.
OMAiPLLHMQE.
OrthoDBiEOG092C3D5H.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di3.40.50.1000. 1 hit.
3.40.50.10190. 1 hit.
InterProiView protein in InterPro
IPR001357. BRCT_dom.
IPR004274. FCP1_dom.
IPR011947. FCP1_euk.
IPR023214. HAD-like_dom.
PfamiView protein in Pfam
PF00533. BRCT. 1 hit.
PF03031. NIF. 1 hit.
SMARTiView protein in SMART
SM00292. BRCT. 1 hit.
SM00577. CPDc. 1 hit.
SUPFAMiSSF52113. SSF52113. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR02250. FCP1_euk. 1 hit.
PROSITEiView protein in PROSITE
PS50172. BRCT. 1 hit.
PS50969. FCP1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03254-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTQIRSPQG LPYPIQIDKL IPSVGSYLHE GDRLLVYKFW YLVERASDTG
60 70 80 90 100
DDDNEHDVSP GGSAGSNGVS PPTKQLRESI EFFESPYEGD LISWNVDVGD
110 120 130 140 150
EVATANQVIC EIKRPCNHDI VYGGLCTQCG KEVSADAFDG VPLDVVGDVD
160 170 180 190 200
LQISETEAIR TGKALKEHLR RDKKLILVVD LDQTIIHCGV DPTIAEWKND
210 220 230 240 250
PNNPNFETLR DVKSFTLDEE LVLPLMYMND DGSMLRPPPV RKCWYYVKVR
260 270 280 290 300
PGLKEFFAKV APLFEMHIYT MATRAYALQI AKIVDPTGEL FGDRILSRDE
310 320 330 340 350
NGSLTTKSLA KLFPTDQSMV VVIDDRGDVW NWCPNLIKVV PYNFFVGVGD
360 370 380 390 400
INSNFLPKQS TGMLQLGRKT RQKSQESQEL LTDIMDNEKK LQEKIDKEVK
410 420 430 440 450
RQEEKLNHQL ATAEEPPANE SKEELTKKLE YSASLEVQQQ NRPLAKLQKH
460 470 480 490 500
LHDQKLLVDD DDELYYLMGT LSNIHKTYYD MLSQQNEPEP NLMEIIPSLK
510 520 530 540 550
QKVFQNCYFV FSGLIPLGTD IQRSDIVIWT STFGATSTPD IDYLTTHLIT
560 570 580 590 600
KNPSTYKARL AKKFNPQIKI VHPDWIFECL VNWKKVDEKP YTLIVDSPIS
610 620 630 640 650
DEELQNFQTQ LQKRQEYLEE TQEQQHMLTS QENLNLFAAG TSWLNNDDDE
660 670 680 690 700
DIPDTASDDD EDDDHDDESD DENNSEGIDR KRSIEDNHDD TSQKKTKAEP
710 720 730
SQDGPVQHKG EGDDNEDSDS QLEEELMDML DD
Length:732
Mass (Da):83,441
Last modified:November 1, 1997 - v1
Checksum:iEACA2A7D33A983C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49704 Genomic DNA. Translation: CAA89775.1.
BK006946 Genomic DNA. Translation: DAA10178.1.
PIRiS54584.
RefSeqiNP_014004.1. NM_001182784.1.

Genome annotation databases

EnsemblFungiiYMR277W; YMR277W; YMR277W.
GeneIDi855320.
KEGGisce:YMR277W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49704 Genomic DNA. Translation: CAA89775.1.
BK006946 Genomic DNA. Translation: DAA10178.1.
PIRiS54584.
RefSeqiNP_014004.1. NM_001182784.1.

3D structure databases

ProteinModelPortaliQ03254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35456. 582 interactors.
DIPiDIP-2330N.
IntActiQ03254. 11 interactors.
MINTiMINT-501044.

PTM databases

iPTMnetiQ03254.

Proteomic databases

MaxQBiQ03254.
PRIDEiQ03254.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR277W; YMR277W; YMR277W.
GeneIDi855320.
KEGGisce:YMR277W.

Organism-specific databases

EuPathDBiFungiDB:YMR277W.
SGDiS000004890. FCP1.

Phylogenomic databases

GeneTreeiENSGT00390000015641.
HOGENOMiHOG000197682.
InParanoidiQ03254.
KOiK15732.
OMAiPLLHMQE.
OrthoDBiEOG092C3D5H.

Enzyme and pathway databases

BioCyciYEAST:G3O-32948-MONOMER.
ReactomeiR-SCE-113418. Formation of the Early Elongation Complex.
R-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-6796648. TP53 Regulates Transcription of DNA Repair Genes.

Miscellaneous databases

PROiPR:Q03254.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di3.40.50.1000. 1 hit.
3.40.50.10190. 1 hit.
InterProiView protein in InterPro
IPR001357. BRCT_dom.
IPR004274. FCP1_dom.
IPR011947. FCP1_euk.
IPR023214. HAD-like_dom.
PfamiView protein in Pfam
PF00533. BRCT. 1 hit.
PF03031. NIF. 1 hit.
SMARTiView protein in SMART
SM00292. BRCT. 1 hit.
SM00577. CPDc. 1 hit.
SUPFAMiSSF52113. SSF52113. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR02250. FCP1_euk. 1 hit.
PROSITEiView protein in PROSITE
PS50172. BRCT. 1 hit.
PS50969. FCP1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFCP1_YEAST
AccessioniPrimary (citable) accession number: Q03254
Secondary accession number(s): D6W0A4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 12, 2017
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.