Q03254 (FCP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA polymerase II subunit A C-terminal domain phosphatase
EC=3.1.3.16

Alternative name(s):
CTD phosphatase FCP1

Gene names

Name:	FCP1
Ordered Locus Names:	YMR277W
ORF Names:	YM8021.03

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II. Ref.4
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Subcellular location	Nucleus.
Miscellaneous	Present with 6550 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 BRCT domain. Contains 1 FCP1 homology domain.

Ontologies

Keywords
Cellular component	Nucleus
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dephosphorylation of RNA polymerase II C-terminal domain Inferred from direct assay Ref.4PubMed 11904169. Source: SGD
Cellular_component	nucleus Inferred from physical interaction PubMed 11003641. Source: SGD
Molecular_function	protein serine/threonine phosphatase activity Inferred from direct assay Ref.4PubMed 11904169. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 732	732	RNA polymerase II subunit A C-terminal domain phosphatase		PRO_0000212568
Regions
	Domain	170 – 363	194	FCP1 homology
	Domain	499 – 593	95	BRCT
Amino acid modifications
	Modified residue	701	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q03254 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: EACA2A7D33A983C6	FASTA	732	83,441
	10 20 30 40 50 60 MTTQIRSPQG LPYPIQIDKL IPSVGSYLHE GDRLLVYKFW YLVERASDTG DDDNEHDVSP 70 80 90 100 110 120 GGSAGSNGVS PPTKQLRESI EFFESPYEGD LISWNVDVGD EVATANQVIC EIKRPCNHDI 130 140 150 160 170 180 VYGGLCTQCG KEVSADAFDG VPLDVVGDVD LQISETEAIR TGKALKEHLR RDKKLILVVD 190 200 210 220 230 240 LDQTIIHCGV DPTIAEWKND PNNPNFETLR DVKSFTLDEE LVLPLMYMND DGSMLRPPPV 250 260 270 280 290 300 RKCWYYVKVR PGLKEFFAKV APLFEMHIYT MATRAYALQI AKIVDPTGEL FGDRILSRDE 310 320 330 340 350 360 NGSLTTKSLA KLFPTDQSMV VVIDDRGDVW NWCPNLIKVV PYNFFVGVGD INSNFLPKQS 370 380 390 400 410 420 TGMLQLGRKT RQKSQESQEL LTDIMDNEKK LQEKIDKEVK RQEEKLNHQL ATAEEPPANE 430 440 450 460 470 480 SKEELTKKLE YSASLEVQQQ NRPLAKLQKH LHDQKLLVDD DDELYYLMGT LSNIHKTYYD 490 500 510 520 530 540 MLSQQNEPEP NLMEIIPSLK QKVFQNCYFV FSGLIPLGTD IQRSDIVIWT STFGATSTPD 550 560 570 580 590 600 IDYLTTHLIT KNPSTYKARL AKKFNPQIKI VHPDWIFECL VNWKKVDEKP YTLIVDSPIS 610 620 630 640 650 660 DEELQNFQTQ LQKRQEYLEE TQEQQHMLTS QENLNLFAAG TSWLNNDDDE DIPDTASDDD 670 680 690 700 710 720 EDDDHDDESD DENNSEGIDR KRSIEDNHDD TSQKKTKAEP SQDGPVQHKG EGDDNEDSDS 730 QLEEELMDML DD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Purification and characterization of an RNA polymerase II phosphatase from yeast." Chambers R.S., Kane C.M. J. Biol. Chem. 271:24498-24504(1996) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[4]	"An unusual eukaryotic protein phosphatase required for transcription by RNA polymerase II and CTD dephosphorylation in S. cerevisiae." Kobor M.S., Archambault J., Lester W., Holstege F.C.P., Gileadi O., Jansma D.B., Jennings E.G., Kouyoumdjian F., Davidson A.R., Young R.A., Greenblatt J. Mol. Cell 4:55-62(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-701, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49704 Genomic DNA. Translation: CAA89775.1. BK006946 Genomic DNA. Translation: DAA10178.1.
PIR	S54584.
RefSeq	NP_014004.1. NM_001182784.1.
3D structure databases
ProteinModelPortal	Q03254.
SMR	Q03254. Positions 160-594.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2330N.
IntAct	Q03254. 11 interactions.
MINT	MINT-501044.
STRING	4932.YMR277W.
Proteomic databases
PaxDb	Q03254.
PeptideAtlas	Q03254.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR277W; YMR277W; YMR277W.
GeneID	855320.
KEGG	sce:YMR277W.
Organism-specific databases
CYGD	YMR277w.
SGD	S000004890. FCP1.
Phylogenomic databases
eggNOG	COG5190.
GeneTree	ENSGT00390000015641.
HOGENOM	HOG000197682.
KO	K15732.
OMA	LYEMHIY.
OrthoDB	EOG4WDHMJ.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32948-MONOMER.
Gene expression databases
Genevestigator	Q03254.
GermOnline	YMR277W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.1000. 3 hits. 3.40.50.10190. 1 hit.
InterPro	IPR001357. BRCT_dom. IPR011947. FCP1_euk. IPR023214. HAD-like_dom. IPR004274. NIF. [Graphical view]
Pfam	PF00533. BRCT. 1 hit. PF03031. NIF. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 1 hit. SM00577. CPDc. 1 hit. [Graphical view]
SUPFAM	SSF52113. BRCT. 1 hit. SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR02250. FCP1_euk. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS50969. FCP1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979023.

Entry information

Entry name

FCP1_YEAST

Accession

Primary (citable) accession number: Q03254
Secondary accession number(s): D6W0A4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 29, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program