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Protein

Glycine-rich RNA-binding protein 7

Gene

RBG7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in RNA transcription or processing during stress. Binds RNAs and DNAs sequence with a preference to single-stranded nucleic acids. Displays strong affinity to poly(U) and poly(G) sequence. Involved in mRNA alternative splicing of numerous targets by modulating splice site selection. Negatively regulates the circadian oscillations of its own transcript as well as RBG8 transcript. Forms an interlocked post-transcriptional negative feedback loop with the RBG8 autoregulatory circuit. Both proteins negatively autoregulate and reciprocally crossregulate by binding to their pre-mRNAs and promoting unproductive splicing coupled to degradation via the NMD pathway. Involved in the regulation of abscisic acid and stress responses. Affects the growth and stress tolerance under high salt and dehydration stress conditions, and also confers freezing tolerance, particularly via the regulation of stomatal opening and closing in the guard cells. Exhibits RNA chaperone activity during the cold adaptation process. Involved in the export of mRNAs from the nucleus to the cytoplasm under cold stress conditions. Target of the Pseudomonas syringae type III effector HopU1, which could probably be involved in plant innate immunity. Component of the flowering autonomous pathway which promotes floral transition, at least partly by down-regulating FLC.15 Publications

GO - Molecular functioni

  • double-stranded DNA binding Source: TAIR
  • mRNA binding Source: TAIR
  • nucleotide binding Source: InterPro
  • RNA binding Source: UniProtKB
  • single-stranded DNA binding Source: TAIR

GO - Biological processi

  • alternative mRNA splicing, via spliceosome Source: TAIR
  • circadian rhythm Source: TAIR
  • DNA duplex unwinding Source: TAIR
  • innate immune response Source: TAIR
  • mRNA export from nucleus Source: TAIR
  • regulation of stomatal movement Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to cold Source: UniProtKB
  • response to cytokinin Source: TAIR
  • response to osmotic stress Source: TAIR
  • response to salt stress Source: UniProtKB
  • response to water deprivation Source: UniProtKB
  • response to zinc ion Source: TAIR
  • RNA secondary structure unwinding Source: TAIR
  • vegetative to reproductive phase transition of meristem Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Immunity, Innate immunity, mRNA processing, mRNA splicing, Plant defense

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine-rich RNA-binding protein 7
Short name:
AtGR-RBP7
Alternative name(s):
AtRBG7
Glycine-rich protein 7
Short name:
AtGRP7
Protein COLD, CIRCADIAN RHYTHM, AND RNA BINDING 2
Short name:
Protein CCR2
Gene namesi
Name:RBG7
Synonyms:CCR2, GR-RBP7, GRP7
Ordered Locus Names:At2g21660
ORF Names:F2G1.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G21660.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Shuttling between nucleus and cytoplasm. Relocalization from the cytoplasm into the nucleus is mediated by TRN1.

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • cytoplasm Source: UniProtKB
  • cytosol Source: TAIR
  • nucleus Source: UniProtKB
  • peroxisome Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Hypersensitive responses to ABA in both seed germination and root growth. Late-flowering. Increased germination rate and seedling growth under salt and dehydration stress. Impaired mRNA export under cold stress conditions. Defective in PAMP-triggered immunity (PTI) responses and high susceptibility to P.syringae and P.carotovorum SCC1 (Pec).6 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47R → K: Abolishes ADP-ribosylation by HopU1. 1 Publication1
Mutagenesisi49R → K: Abolishes ADP-ribosylation by HopU1. Enable to complement the rbg7 mutant. 4 Publications1
Mutagenesisi49R → Q: Impairs RNA-binding and consequently impairs the regulation of its pre-mRNA and its downstream pre-mRNA target RBG8. Affects the alternative splicing of numerous targets. 4 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000815992 – 176Glycine-rich RNA-binding protein 7Add BLAST175

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei49ADP-ribosylarginine; by HopU11 Publication1
Modified residuei105PhosphoserineCombined sources1
Modified residuei117PhosphoserineCombined sources1

Post-translational modificationi

ADP-ribosylated by the Pseudomonas syringae type III effector HopU1. ADP-ribosylation reduces the ability of the protein to bind RNA.1 Publication

Keywords - PTMi

Acetylation, ADP-ribosylation, Phosphoprotein

Proteomic databases

PaxDbiQ03250.
PRIDEiQ03250.
ProMEXiQ03250.

PTM databases

iPTMnetiQ03250.
SwissPalmiQ03250.

Expressioni

Tissue specificityi

Ubiquitous with strong expression in guard cell.2 Publications

Inductioni

Up-regulated by cold stress and down-regulated by dehydration stress, salt stress, abscisic acid (ABA) and mannitol. Circadian regulation. Induced by hydrogen peroxide (at the protein level). Up-regulated under P.carotovorum SCC1 (Pec) infection.8 Publications

Gene expression databases

ExpressionAtlasiQ03250. baseline and differential.
GenevisibleiQ03250. AT.

Interactioni

Subunit structurei

Interacts with TRN1. Interacts with the Pseudomonas syringae type III effector HopU1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
EFRC0LGT64EBI-1393626,EBI-8801168
FLS2Q9FL284EBI-1393626,EBI-1799448
hopU1Q88A912EBI-1393626,EBI-8802399From a different organism.

Protein-protein interaction databases

BioGridi2058. 8 interactors.
IntActiQ03250. 41 interactors.
MINTiMINT-1163877.
STRINGi3702.AT2G21660.1.

Structurei

3D structure databases

ProteinModelPortaliQ03250.
SMRiQ03250.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 86RRMPROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 41Required for RNA chaperone activityAdd BLAST40
Regioni97 – 148Nuclear targeting sequence (M9)Add BLAST52

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi88 – 175Gly-richAdd BLAST88

Domaini

N-terminal part of the protein is one of the crucial determinant to confer RNA chaperone activity during cold adaptation process.1 Publication

Sequence similaritiesi

Belongs to the GR-RBP family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
HOGENOMiHOG000276232.
InParanoidiQ03250.
OMAiFKDEQAM.
OrthoDBiEOG09360WUX.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q03250-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASGDVEYRC FVGGLAWATD DRALETAFAQ YGDVIDSKII NDRETGRSRG
60 70 80 90 100
FGFVTFKDEK AMKDAIEGMN GQDLDGRSIT VNEAQSRGSG GGGGHRGGGG
110 120 130 140 150
GGYRSGGGGG YSGGGGSYGG GGGRREGGGG YSGGGGGYSS RGGGGGSYGG
160 170
GRREGGGGYG GGEGGGYGGS GGGGGW
Length:176
Mass (Da):16,890
Last modified:June 1, 1994 - v1
Checksum:i3E1025477F9CF4C4
GO
Isoform 2 (identifier: Q03250-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     39-48: IINDRETGRS → VCYTPRSDSE
     49-176: Missing.

Note: May be due to a competing donor splice site. No experimental confirmation available.
Show »
Length:48
Mass (Da):5,268
Checksum:i2EA39A77DA892484
GO

Sequence cautioni

The sequence AAK68766 differs from that shown. Wrong choice of frame.Curated
The sequence AAL66938 differs from that shown. Wrong choice of frame.Curated
The sequence BAH57083 differs from that shown. Wrong choice of frame.Curated
The sequence L04172 differs from that shown. Reason: Frameshift at position 11.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti133Missing in AAM62447 (Ref. 7) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04585539 – 48IINDRETGRS → VCYTPRSDSE in isoform 2. 3 Publications10
Alternative sequenceiVSP_04585649 – 176Missing in isoform 2. 3 PublicationsAdd BLAST128

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14987 mRNA. Translation: CAA78711.1.
L00648 mRNA. Translation: AAA32853.1.
L04172 mRNA. No translation available.
AC007119 Genomic DNA. Translation: AAD23639.1.
CP002685 Genomic DNA. Translation: AEC07209.1.
AF428381 mRNA. Translation: AAL16149.1.
AY054284 mRNA. Translation: AAL06943.1.
AY042826 mRNA. Translation: AAK68766.1. Sequence problems.
AY072523 mRNA. Translation: AAL66938.1. Sequence problems.
AK318968 mRNA. Translation: BAH57083.1. Sequence problems.
AY085214 mRNA. Translation: AAM62447.1.
PIRiS30147.
RefSeqiNP_179760.1. NM_127738.5. [Q03250-1]
UniGeneiAt.22672.
At.23628.
At.24279.
At.72988.

Genome annotation databases

EnsemblPlantsiAT2G21660.1; AT2G21660.1; AT2G21660. [Q03250-1]
GeneIDi816705.
GrameneiAT2G21660.1; AT2G21660.1; AT2G21660.
KEGGiath:AT2G21660.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14987 mRNA. Translation: CAA78711.1.
L00648 mRNA. Translation: AAA32853.1.
L04172 mRNA. No translation available.
AC007119 Genomic DNA. Translation: AAD23639.1.
CP002685 Genomic DNA. Translation: AEC07209.1.
AF428381 mRNA. Translation: AAL16149.1.
AY054284 mRNA. Translation: AAL06943.1.
AY042826 mRNA. Translation: AAK68766.1. Sequence problems.
AY072523 mRNA. Translation: AAL66938.1. Sequence problems.
AK318968 mRNA. Translation: BAH57083.1. Sequence problems.
AY085214 mRNA. Translation: AAM62447.1.
PIRiS30147.
RefSeqiNP_179760.1. NM_127738.5. [Q03250-1]
UniGeneiAt.22672.
At.23628.
At.24279.
At.72988.

3D structure databases

ProteinModelPortaliQ03250.
SMRiQ03250.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2058. 8 interactors.
IntActiQ03250. 41 interactors.
MINTiMINT-1163877.
STRINGi3702.AT2G21660.1.

PTM databases

iPTMnetiQ03250.
SwissPalmiQ03250.

Proteomic databases

PaxDbiQ03250.
PRIDEiQ03250.
ProMEXiQ03250.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G21660.1; AT2G21660.1; AT2G21660. [Q03250-1]
GeneIDi816705.
GrameneiAT2G21660.1; AT2G21660.1; AT2G21660.
KEGGiath:AT2G21660.

Organism-specific databases

TAIRiAT2G21660.

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
HOGENOMiHOG000276232.
InParanoidiQ03250.
OMAiFKDEQAM.
OrthoDBiEOG09360WUX.

Miscellaneous databases

PROiQ03250.

Gene expression databases

ExpressionAtlasiQ03250. baseline and differential.
GenevisibleiQ03250. AT.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBG7_ARATH
AccessioniPrimary (citable) accession number: Q03250
Secondary accession number(s): C0Z304, Q8LEV4, Q94B62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants overexpressing RBG7 display retarded germination and affected seedling growth under salt and dehydration stress conditions, confer freezing tolerance and also possess enhanced resistance to P.syringae.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.