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Protein

Glycine-rich RNA-binding protein 7

Gene

RBG7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in RNA transcription or processing during stress. Binds RNAs and DNAs sequence with a preference to single-stranded nucleic acids. Displays strong affinity to poly(U) and poly(G) sequence. Involved in mRNA alternative splicing of numerous targets by modulating splice site selection. Negatively regulates the circadian oscillations of its own transcript as well as RBG8 transcript. Forms an interlocked post-transcriptional negative feedback loop with the RBG8 autoregulatory circuit. Both proteins negatively autoregulate and reciprocally crossregulate by binding to their pre-mRNAs and promoting unproductive splicing coupled to degradation via the NMD pathway. Involved in the regulation of abscisic acid and stress responses. Affects the growth and stress tolerance under high salt and dehydration stress conditions, and also confers freezing tolerance, particularly via the regulation of stomatal opening and closing in the guard cells. Exhibits RNA chaperone activity during the cold adaptation process. Involved in the export of mRNAs from the nucleus to the cytoplasm under cold stress conditions. Target of the Pseudomonas syringae type III effector HopU1, which could probably be involved in plant innate immunity. Component of the flowering autonomous pathway which promotes floral transition, at least partly by down-regulating FLC.15 Publications

Miscellaneous

Plants overexpressing RBG7 display retarded germination and affected seedling growth under salt and dehydration stress conditions, confer freezing tolerance and also possess enhanced resistance to P.syringae.

GO - Molecular functioni

  • double-stranded DNA binding Source: TAIR
  • mRNA binding Source: TAIR
  • RNA binding Source: UniProtKB
  • single-stranded DNA binding Source: TAIR

GO - Biological processi

  • alternative mRNA splicing, via spliceosome Source: TAIR
  • circadian rhythm Source: TAIR
  • DNA duplex unwinding Source: TAIR
  • innate immune response Source: TAIR
  • mRNA export from nucleus Source: TAIR
  • regulation of stomatal movement Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to cold Source: UniProtKB
  • response to cytokinin Source: TAIR
  • response to osmotic stress Source: TAIR
  • response to salt stress Source: UniProtKB
  • response to water deprivation Source: UniProtKB
  • response to zinc ion Source: TAIR
  • RNA secondary structure unwinding Source: TAIR
  • vegetative to reproductive phase transition of meristem Source: TAIR

Keywordsi

Molecular functionChaperone, RNA-binding
Biological processImmunity, Innate immunity, mRNA processing, mRNA splicing, Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine-rich RNA-binding protein 7
Short name:
AtGR-RBP7
Alternative name(s):
AtRBG7
Glycine-rich protein 7
Short name:
AtGRP7
Protein COLD, CIRCADIAN RHYTHM, AND RNA BINDING 2
Short name:
Protein CCR2
Gene namesi
Name:RBG7
Synonyms:CCR2, GR-RBP7, GRP7
Ordered Locus Names:At2g21660
ORF Names:F2G1.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G21660
TAIRilocus:2049359 AT2G21660

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Hypersensitive responses to ABA in both seed germination and root growth. Late-flowering. Increased germination rate and seedling growth under salt and dehydration stress. Impaired mRNA export under cold stress conditions. Defective in PAMP-triggered immunity (PTI) responses and high susceptibility to P.syringae and P.carotovorum SCC1 (Pec).6 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47R → K: Abolishes ADP-ribosylation by HopU1. 1 Publication1
Mutagenesisi49R → K: Abolishes ADP-ribosylation by HopU1. Enable to complement the rbg7 mutant. 4 Publications1
Mutagenesisi49R → Q: Impairs RNA-binding and consequently impairs the regulation of its pre-mRNA and its downstream pre-mRNA target RBG8. Affects the alternative splicing of numerous targets. 4 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000815992 – 176Glycine-rich RNA-binding protein 7Add BLAST175

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei49ADP-ribosylarginine; by HopU11 Publication1
Modified residuei105PhosphoserineCombined sources1
Modified residuei117PhosphoserineCombined sources1

Post-translational modificationi

ADP-ribosylated by the Pseudomonas syringae type III effector HopU1. ADP-ribosylation reduces the ability of the protein to bind RNA.1 Publication

Keywords - PTMi

Acetylation, ADP-ribosylation, Phosphoprotein

Proteomic databases

PaxDbiQ03250
PRIDEiQ03250
ProMEXiQ03250

PTM databases

iPTMnetiQ03250
SwissPalmiQ03250

Expressioni

Tissue specificityi

Ubiquitous with strong expression in guard cell.2 Publications

Inductioni

Up-regulated by cold stress and down-regulated by dehydration stress, salt stress, abscisic acid (ABA) and mannitol. Circadian regulation. Induced by hydrogen peroxide (at the protein level). Up-regulated under P.carotovorum SCC1 (Pec) infection.8 Publications

Gene expression databases

ExpressionAtlasiQ03250 baseline and differential
GenevisibleiQ03250 AT

Interactioni

Subunit structurei

Interacts with TRN1. Interacts with the Pseudomonas syringae type III effector HopU1.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi2058, 8 interactors
IntActiQ03250, 41 interactors
MINTiQ03250
STRINGi3702.AT2G21660.1

Structurei

3D structure databases

ProteinModelPortaliQ03250
SMRiQ03250
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 86RRMPROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 41Required for RNA chaperone activityAdd BLAST40
Regioni97 – 148Nuclear targeting sequence (M9)Add BLAST52

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi88 – 175Gly-richAdd BLAST88

Domaini

N-terminal part of the protein is one of the crucial determinant to confer RNA chaperone activity during cold adaptation process.1 Publication

Sequence similaritiesi

Belongs to the GR-RBP family.Curated

Phylogenomic databases

eggNOGiKOG0118 Eukaryota
COG0724 LUCA
HOGENOMiHOG000276232
InParanoidiQ03250
OMAiMGEACKV
OrthoDBiEOG09360WUX

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q03250-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASGDVEYRC FVGGLAWATD DRALETAFAQ YGDVIDSKII NDRETGRSRG
60 70 80 90 100
FGFVTFKDEK AMKDAIEGMN GQDLDGRSIT VNEAQSRGSG GGGGHRGGGG
110 120 130 140 150
GGYRSGGGGG YSGGGGSYGG GGGRREGGGG YSGGGGGYSS RGGGGGSYGG
160 170
GRREGGGGYG GGEGGGYGGS GGGGGW
Length:176
Mass (Da):16,890
Last modified:June 1, 1994 - v1
Checksum:i3E1025477F9CF4C4
GO
Isoform 2 (identifier: Q03250-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     39-48: IINDRETGRS → VCYTPRSDSE
     49-176: Missing.

Note: May be due to a competing donor splice site. No experimental confirmation available.
Show »
Length:48
Mass (Da):5,268
Checksum:i2EA39A77DA892484
GO

Sequence cautioni

The sequence AAK68766 differs from that shown. Wrong choice of frame.Curated
The sequence AAL66938 differs from that shown. Wrong choice of frame.Curated
The sequence BAH57083 differs from that shown. Wrong choice of frame.Curated
The sequence L04172 differs from that shown. Reason: Frameshift at position 11.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti133Missing in AAM62447 (Ref. 7) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04585539 – 48IINDRETGRS → VCYTPRSDSE in isoform 2. 3 Publications10
Alternative sequenceiVSP_04585649 – 176Missing in isoform 2. 3 PublicationsAdd BLAST128

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14987 mRNA Translation: CAA78711.1
L00648 mRNA Translation: AAA32853.1
L04172 mRNA No translation available.
AC007119 Genomic DNA Translation: AAD23639.1
CP002685 Genomic DNA Translation: AEC07209.1
AF428381 mRNA Translation: AAL16149.1
AY054284 mRNA Translation: AAL06943.1
AY042826 mRNA Translation: AAK68766.1 Sequence problems.
AY072523 mRNA Translation: AAL66938.1 Sequence problems.
AK318968 mRNA Translation: BAH57083.1 Sequence problems.
AY085214 mRNA Translation: AAM62447.1
PIRiS30147
RefSeqiNP_179760.1, NM_127738.5 [Q03250-1]
UniGeneiAt.22672
At.23628
At.24279
At.72988

Genome annotation databases

EnsemblPlantsiAT2G21660.1; AT2G21660.1; AT2G21660 [Q03250-1]
AT2G21660.2; AT2G21660.2; AT2G21660
GeneIDi816705
GrameneiAT2G21660.1; AT2G21660.1; AT2G21660 [Q03250-1]
AT2G21660.2; AT2G21660.2; AT2G21660
KEGGiath:AT2G21660

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRBG7_ARATH
AccessioniPrimary (citable) accession number: Q03250
Secondary accession number(s): C0Z304, Q8LEV4, Q94B62
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 25, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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