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Protein

Apolipoprotein E

Gene

APOE

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipoprotein E
Short name:
Apo-E
Gene namesi
Name:APOE
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chylomicron, HDL, Secreted, VLDL

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818By similarityAdd
BLAST
Chaini19 – 316298Apolipoprotein EPRO_0000001984Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi32 – 321O-linked (GalNAc...)1 Publication
Modified residuei142 – 1421Methionine sulfoxideBy similarity
Modified residuei146 – 1461PhosphoserineBy similarity
Glycosylationi211 – 2111O-linked (GalNAc...)1 Publication
Glycosylationi309 – 3091O-linked (GalNAc...)1 Publication
Glycosylationi310 – 3101O-linked (GalNAc...)1 Publication

Post-translational modificationi

Phosphorylated by FAM20C in the extracellular medium.By similarity

Keywords - PTMi

Glycoprotein, Oxidation, Phosphoprotein

Proteomic databases

PaxDbiQ03247.
PRIDEiQ03247.

PTM databases

UniCarbKBiQ03247.

Expressioni

Tissue specificityi

Secreted in plasma.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013354.

Structurei

3D structure databases

ProteinModelPortaliQ03247.
SMRiQ03247. Positions 29-202.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati79 – 100221Add
BLAST
Repeati101 – 122222Add
BLAST
Repeati123 – 144223Add
BLAST
Repeati145 – 166224Add
BLAST
Repeati167 – 188225Add
BLAST
Repeati189 – 210226Add
BLAST
Repeati211 – 232227Add
BLAST
Repeati233 – 254228Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni79 – 2541768 X 22 AA approximate tandem repeatsAdd
BLAST
Regioni157 – 16711LDL receptor bindingBy similarityAdd
BLAST
Regioni161 – 1644Heparin-bindingBy similarity
Regioni228 – 2358Heparin-bindingBy similarity

Sequence similaritiesi

Belongs to the apolipoprotein A1/A4/E family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IVK0. Eukaryota.
ENOG4111MYC. LUCA.
HOGENOMiHOG000034006.
HOVERGENiHBG010582.
InParanoidiQ03247.
KOiK04524.
OrthoDBiEOG793B87.
TreeFamiTF334458.

Family and domain databases

InterProiIPR000074. ApoA_E.
[Graphical view]
PfamiPF01442. Apolipoprotein. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03247-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLWVAVVV ALLAGCQADM EGELGPEEPL TTQQPRGKDS QPWEQALGRF
60 70 80 90 100
WDYLRWVQTL SDQVQEELLN TQVIQELTAL MEETMKEVKA YKEELEGQLG
110 120 130 140 150
PMAQETQARV SKELQAAQAR LGSDMEDLRN RLAQYRSEVQ AMLGQSTEEL
160 170 180 190 200
RARMASHLRK LPKRLLRDAD DLKKRLAVYQ AGASEGAERS LSAIRERFGP
210 220 230 240 250
LVEQGQSRAA TLSTLAGQPL LERAEAWRQK LHGRLEEVGV RAQDRLDKIR
260 270 280 290 300
QQLEEVHAKV EEQGNQMRLQ AEAFQARLRS WFEPLVEDMQ RQWAGLVEKV
310
QLALRPSPTS PPSENH
Length:316
Mass (Da):35,980
Last modified:October 1, 1993 - v1
Checksum:i1231292FC8DA9F1E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti129 – 1302RN → CG in CAA43479 (PubMed:1908018).Curated
Sequence conflicti162 – 1621P → R in AAX09070 (PubMed:16305752).Curated
Sequence conflicti162 – 1621P → R in AAI02621 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64839 mRNA. Translation: CAA46051.1.
X61171 mRNA. Translation: CAA43479.1.
BT021053 mRNA. Translation: AAX09070.1.
BC102620 mRNA. Translation: AAI02621.1.
PIRiI45996.
S26478.
RefSeqiNP_776416.1. NM_173991.2.
UniGeneiBt.34980.

Genome annotation databases

GeneIDi281004.
KEGGibta:281004.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64839 mRNA. Translation: CAA46051.1.
X61171 mRNA. Translation: CAA43479.1.
BT021053 mRNA. Translation: AAX09070.1.
BC102620 mRNA. Translation: AAI02621.1.
PIRiI45996.
S26478.
RefSeqiNP_776416.1. NM_173991.2.
UniGeneiBt.34980.

3D structure databases

ProteinModelPortaliQ03247.
SMRiQ03247. Positions 29-202.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013354.

PTM databases

UniCarbKBiQ03247.

Proteomic databases

PaxDbiQ03247.
PRIDEiQ03247.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281004.
KEGGibta:281004.

Organism-specific databases

CTDi348.

Phylogenomic databases

eggNOGiENOG410IVK0. Eukaryota.
ENOG4111MYC. LUCA.
HOGENOMiHOG000034006.
HOVERGENiHBG010582.
InParanoidiQ03247.
KOiK04524.
OrthoDBiEOG793B87.
TreeFamiTF334458.

Miscellaneous databases

NextBioi20805102.

Family and domain databases

InterProiIPR000074. ApoA_E.
[Graphical view]
PfamiPF01442. Apolipoprotein. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, sequencing, and expression analysis of a bovine apolipoprotein E (APOE) cDNA and chromosomal localization of the APOE locus."
    Brzozowska A., Fries R., Womack J.E., Grimholt U., Myklebost O., Rogne S.
    Mamm. Genome 4:53-57(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Cloning and sequencing of bovine apolipoprotein E complementary DNA and molecular evolution of apolipoproteins E, C-I, and C-II."
    Yang Y.W., Chan L., Li W.H.
    J. Mol. Evol. 32:469-475(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Testis.
  5. "Affinity enrichment and characterization of mucin core-1 type glycopeptides from bovine serum."
    Darula Z., Medzihradszky K.F.
    Mol. Cell. Proteomics 8:2515-2526(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT THR-32; THR-211; THR-309 AND SER-310, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiAPOE_BOVIN
AccessioniPrimary (citable) accession number: Q03247
Secondary accession number(s): Q3T006, Q5E967
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 13, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.