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Protein

Non-structural protein 2

Gene
N/A
Organism
Rotavirus A (strain RVA/Human/United States/Wa/1974/G1P1A[8]) (RV-A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Participates in replication and packaging of the viral genome. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the host cytoplasm where replication intermediates are assembled and viral RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activities. The unwinding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. The RTPase activity plays a role in the removal of the gamma-phosphate from the rotavirus RNA minus strands of dsRNA genome segments.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei188ATPUniRule annotation1
Active sitei225For NTPase and RTPase activitiesUniRule annotation1
Binding sitei227ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi107 – 109ATPUniRule annotation3
Nucleotide bindingi221 – 223ATPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, RNA-binding
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 2UniRule annotation (EC:3.6.4.-UniRule annotation)
Short name:
NSP2UniRule annotation
Alternative name(s):
NCVP3UniRule annotation
Non-structural RNA-binding protein 35UniRule annotation
Short name:
NS35UniRule annotation
OrganismiRotavirus A (strain RVA/Human/United States/Wa/1974/G1P1A[8]) (RV-A)
Taxonomic identifieri10962 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000006581 Componenti: Genome

Subcellular locationi

  • Host cytoplasm UniRule annotation
  • Note: Found in spherical cytoplasmic structures, called viral factories, that appear early after infection and are the site of viral replication and packaging.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001495481 – 317Non-structural protein 2Add BLAST317

Interactioni

Subunit structurei

Homooctamer. Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 phosphorylation and formation of viral factories.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ03245
SMRiQ03245
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni205 – 241RNA-bindingUniRule annotationAdd BLAST37

Sequence similaritiesi

Belongs to the rotavirus NSP2 family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG090000GH

Family and domain databases

Gene3Di3.30.428.20, 1 hit
3.90.1400.10, 1 hit
HAMAPiMF_04089 ROTA_NSP2, 1 hit
InterProiView protein in InterPro
IPR003668 Rotavirus_NSP2
IPR024076 Rotavirus_NSP2_C
IPR024068 Rotavirus_NSP2_N
PfamiView protein in Pfam
PF02509 Rota_NS35, 1 hit
SUPFAMiSSF75347 SSF75347, 1 hit
SSF75574 SSF75574, 1 hit

Sequencei

Sequence statusi: Complete.

Q03245-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELACFCYP HLENDSYKFI PFNNLAIKCM LTAKVEKKDQ DKFYNSIVYG
60 70 80 90 100
IAPPPQFKKR YNTSDNSRGM NYETIMFNKV AVLICEALNS IKITQSDVAN
110 120 130 140 150
VLSRVVSVRH LENLVLRKEN HQDVLFHSKE LLLKSVLIAI GQSKEIETTA
160 170 180 190 200
TAEGGEIVFQ NAAFTMWKLT YLDHKLMPIL DQNFIEYKIT LNEDKPISDV
210 220 230 240 250
CIKELVAELR WQYNRFAVIT HGKGHYRVVK YSSVANHADR VFATYKNSAK
260 270 280 290 300
SGNVIDFNLL DQRIIWQNWY AFTSSMKQGN TLDVCKKLLF QKMKQEKNPF
310
KGLSTDRKMD EVSHVGI
Length:317
Mass (Da):36,541
Last modified:June 1, 1994 - v1
Checksum:i343C247E52EAD4F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04534 Genomic RNA Translation: AAA47301.1

Similar proteinsi

Entry informationi

Entry nameiNSP2_ROTHW
AccessioniPrimary (citable) accession number: Q03245
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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