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Q03243

- NSP2_ROTSR

UniProt

Q03243 - NSP2_ROTSR

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Protein

Non-structural protein 2

Gene
N/A
Organism
Rotavirus A (strain SA11-Ramig G3-Px[x]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A) (Simian Agent 11 (strain Ramig))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments (By similarity).By similarity

Cofactori

Magnesium for NTPase activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi153 – 1531MagnesiumSequence Analysis
Metal bindingi171 – 1711MagnesiumSequence Analysis
Active sitei225 – 2251For NTPase/RTPase activity1 Publication

GO - Molecular functioni

  1. hydrolase activity, acting on acid anhydrides Source: InterPro
  2. metal ion binding Source: UniProtKB-KW
  3. nucleotide binding Source: UniProtKB-KW
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 2 (EC:3.6.4.-)
Short name:
NSP2
Alternative name(s):
NCVP3
Non-structural RNA-binding protein 35
Short name:
NS35
OrganismiRotavirus A (strain SA11-Ramig G3-Px[x]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A) (Simian Agent 11 (strain Ramig))
Taxonomic identifieri36435 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]

Subcellular locationi

Host cytoplasm Curated
Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Non-structural protein 2PRO_0000149552Add
BLAST

Interactioni

Subunit structurei

Homooctamer. Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories (By similarity).By similarity

Structurei

Secondary structure

1
317
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63
Beta strandi8 – 136
Beta strandi16 – 216
Helixi24 – 318
Helixi37 – 393
Beta strandi47 – 493
Helixi55 – 606
Beta strandi68 – 703
Helixi75 – 8915
Turni90 – 923
Turni95 – 973
Helixi98 – 1036
Helixi108 – 11811
Helixi124 – 1274
Helixi129 – 13911
Helixi149 – 1513
Beta strandi156 – 1605
Beta strandi162 – 1687
Helixi171 – 1733
Beta strandi175 – 1773
Beta strandi186 – 1916
Helixi199 – 21214
Turni213 – 2153
Beta strandi216 – 2194
Beta strandi222 – 2309
Helixi231 – 2333
Helixi234 – 25118
Turni262 – 2643
Helixi267 – 27711
Helixi282 – 2898
Beta strandi295 – 2973
Turni299 – 3024
Helixi303 – 31210

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1L9VX-ray2.60A1-317[»]
2R7CX-ray2.70A1-317[»]
2R7JX-ray2.60A1-317[»]
2R7PX-ray2.80A1-317[»]
2R8FX-ray2.80A1-317[»]
4G0AX-ray2.10A/B/C/D1-317[»]
4G0JX-ray3.40A/B/C/D/E/F/G/H/I/J1-294[»]
ProteinModelPortaliQ03243.
SMRiQ03243. Positions 1-313.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03243.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni205 – 24137RNA-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus NSP2 family.Curated

Family and domain databases

Gene3Di3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProiIPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view]
PfamiPF02509. Rota_NS35. 1 hit.
[Graphical view]
SUPFAMiSSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03243-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAELACFCYP HLENDSYKFI PFNNLAIKAM LTAKVDKKDM DKFYDSIIYG
60 70 80 90 100
IAPPPQFKKR YNTNDNSRGM NFETIMFTKV AMLICEALNS LKVTQANVSN
110 120 130 140 150
VLSRVVSIRH LENLVIRKEN PQDILFHSKD LLLKSTLIAI GQSKEIETTI
160 170 180 190 200
TAEGGEIVFQ NAAFTMWKLT YLEHQLMPIL DQNFIEYKVT LNEDKPISDV
210 220 230 240 250
HVKELVAELR WQYNKFAVIT HGKGHYRIVK YSSVANHADR VYATFKSNVK
260 270 280 290 300
TGVNNDFNLL DQRIIWQNWY AFTSSMKQGN TLDVCKRLLF QKMKPEKNPF
310
KGLSTDRKMD EVSQVGV
Length:317
Mass (Da):36,569
Last modified:June 1, 1994 - v1
Checksum:i3D7BEA9514934E62
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L04532 Genomic RNA. Translation: AAA47299.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L04532 Genomic RNA. Translation: AAA47299.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1L9V X-ray 2.60 A 1-317 [» ]
2R7C X-ray 2.70 A 1-317 [» ]
2R7J X-ray 2.60 A 1-317 [» ]
2R7P X-ray 2.80 A 1-317 [» ]
2R8F X-ray 2.80 A 1-317 [» ]
4G0A X-ray 2.10 A/B/C/D 1-317 [» ]
4G0J X-ray 3.40 A/B/C/D/E/F/G/H/I/J 1-294 [» ]
ProteinModelPortali Q03243.
SMRi Q03243. Positions 1-313.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q03243.

Family and domain databases

Gene3Di 3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProi IPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view ]
Pfami PF02509. Rota_NS35. 1 hit.
[Graphical view ]
SUPFAMi SSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide and amino acid sequence analysis of the rotavirus nonstructural RNA-binding protein NS35."
    Patton J.T., Salter-Cid L., Kalbach A.N., Mansell E.A., Kattoura M.D.
    Virology 192:438-446(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Rotavirus protein involved in genome replication and packaging exhibits a HIT-like fold."
    Jayaram H., Taraporewala Z.F., Patton J.T., Prasad B.V.V.
    Nature 417:311-315(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
  3. "Crystallographic and biochemical analysis of rotavirus NSP2 with nucleotides reveals a nucleoside diphosphate kinase-like activity."
    Kumar M., Jayaram H., Vasquez-Del Carpio R., Jiang X., Taraporewala Z.F., Jacobson R.H., Patton J.T., Prasad B.V.V.
    J. Virol. 81:12272-12284(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS), ACTIVE SITE.

Entry informationi

Entry nameiNSP2_ROTSR
AccessioniPrimary (citable) accession number: Q03243
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3