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Protein

Non-structural protein 2

Gene
N/A
Organism
Rotavirus A (strain RVA/SA11-Ramig/G3P[X]) (RV-A) (Simian Agent 11 (strain Ramig))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments (By similarity).By similarity

Cofactori

Mg2+By similarityNote: Magnesium is required for NTPase activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi153MagnesiumSequence analysis1
Metal bindingi171MagnesiumSequence analysis1
Active sitei225For NTPase/RTPase activity1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 2 (EC:3.6.4.-)
Short name:
NSP2
Alternative name(s):
NCVP3
Non-structural RNA-binding protein 35
Short name:
NS35
OrganismiRotavirus A (strain RVA/SA11-Ramig/G3P[X]) (RV-A) (Simian Agent 11 (strain Ramig))
Taxonomic identifieri36435 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]

Subcellular locationi

  • Host cytoplasm Curated

  • Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001495521 – 317Non-structural protein 2Add BLAST317

Interactioni

Subunit structurei

Homooctamer. Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories (By similarity).By similarity

Structurei

Secondary structure

1317
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 6Combined sources3
Beta strandi8 – 13Combined sources6
Beta strandi16 – 21Combined sources6
Helixi24 – 31Combined sources8
Helixi37 – 39Combined sources3
Beta strandi47 – 49Combined sources3
Helixi55 – 60Combined sources6
Beta strandi68 – 70Combined sources3
Helixi75 – 89Combined sources15
Turni90 – 92Combined sources3
Turni95 – 97Combined sources3
Helixi98 – 103Combined sources6
Helixi108 – 118Combined sources11
Helixi124 – 127Combined sources4
Helixi129 – 139Combined sources11
Helixi149 – 151Combined sources3
Beta strandi156 – 160Combined sources5
Beta strandi162 – 168Combined sources7
Helixi171 – 173Combined sources3
Beta strandi175 – 177Combined sources3
Beta strandi186 – 191Combined sources6
Helixi199 – 212Combined sources14
Turni213 – 215Combined sources3
Beta strandi216 – 219Combined sources4
Beta strandi222 – 230Combined sources9
Helixi231 – 233Combined sources3
Helixi234 – 251Combined sources18
Turni262 – 264Combined sources3
Helixi267 – 277Combined sources11
Helixi282 – 289Combined sources8
Beta strandi295 – 297Combined sources3
Turni299 – 302Combined sources4
Helixi303 – 312Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1L9VX-ray2.60A1-317[»]
2R7CX-ray2.70A1-317[»]
2R7JX-ray2.60A1-317[»]
2R7PX-ray2.80A1-317[»]
2R8FX-ray2.80A1-317[»]
4G0AX-ray2.10A/B/C/D1-317[»]
4G0JX-ray3.40A/B/C/D/E/F/G/H/I/J1-294[»]
ProteinModelPortaliQ03243.
SMRiQ03243.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ03243.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni205 – 241RNA-bindingSequence analysisAdd BLAST37

Sequence similaritiesi

Belongs to the rotavirus NSP2 family.Curated

Family and domain databases

Gene3Di3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProiIPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view]
PfamiPF02509. Rota_NS35. 1 hit.
[Graphical view]
SUPFAMiSSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03243-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELACFCYP HLENDSYKFI PFNNLAIKAM LTAKVDKKDM DKFYDSIIYG
60 70 80 90 100
IAPPPQFKKR YNTNDNSRGM NFETIMFTKV AMLICEALNS LKVTQANVSN
110 120 130 140 150
VLSRVVSIRH LENLVIRKEN PQDILFHSKD LLLKSTLIAI GQSKEIETTI
160 170 180 190 200
TAEGGEIVFQ NAAFTMWKLT YLEHQLMPIL DQNFIEYKVT LNEDKPISDV
210 220 230 240 250
HVKELVAELR WQYNKFAVIT HGKGHYRIVK YSSVANHADR VYATFKSNVK
260 270 280 290 300
TGVNNDFNLL DQRIIWQNWY AFTSSMKQGN TLDVCKRLLF QKMKPEKNPF
310
KGLSTDRKMD EVSQVGV
Length:317
Mass (Da):36,569
Last modified:June 1, 1994 - v1
Checksum:i3D7BEA9514934E62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04532 Genomic RNA. Translation: AAA47299.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04532 Genomic RNA. Translation: AAA47299.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1L9VX-ray2.60A1-317[»]
2R7CX-ray2.70A1-317[»]
2R7JX-ray2.60A1-317[»]
2R7PX-ray2.80A1-317[»]
2R8FX-ray2.80A1-317[»]
4G0AX-ray2.10A/B/C/D1-317[»]
4G0JX-ray3.40A/B/C/D/E/F/G/H/I/J1-294[»]
ProteinModelPortaliQ03243.
SMRiQ03243.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ03243.

Family and domain databases

Gene3Di3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProiIPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view]
PfamiPF02509. Rota_NS35. 1 hit.
[Graphical view]
SUPFAMiSSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNSP2_ROTSR
AccessioniPrimary (citable) accession number: Q03243
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.