Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Non-structural protein 2

Gene
N/A
Organism
Rotavirus A (strain RVA/Human/United States/DS-1/1976/G2P1B[4]) (RV-A) (Rotavirus A (strain DS1))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Participates in replication and packaging of the viral genome. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the host cytoplasm where replication intermediates are assembled and viral RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activities. The unwinding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. The RTPase activity plays a role in the removal of the gamma-phosphate from the rotavirus RNA minus strands of dsRNA genome segments.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei188ATPUniRule annotation1
Active sitei225For NTPase and RTPase activitiesUniRule annotation1
Binding sitei227ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi107 – 109ATPUniRule annotation3
Nucleotide bindingi221 – 223ATPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, RNA-binding
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 2UniRule annotation (EC:3.6.4.-UniRule annotation)
Short name:
NSP2UniRule annotation
Alternative name(s):
NCVP3UniRule annotation
Non-structural RNA-binding protein 35UniRule annotation
Short name:
NS35UniRule annotation
OrganismiRotavirus A (strain RVA/Human/United States/DS-1/1976/G2P1B[4]) (RV-A) (Rotavirus A (strain DS1))
Taxonomic identifieri10950 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000001457 Componenti: Genome

Subcellular locationi

  • Host cytoplasm UniRule annotation
  • Note: Found in spherical cytoplasmic structures, called viral factories, that appear early after infection and are the site of viral replication and packaging.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001495471 – 317Non-structural protein 2Add BLAST317

Proteomic databases

PRIDEiQ03240

Interactioni

Subunit structurei

Homooctamer. Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 phosphorylation and formation of viral factories.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ03240
SMRiQ03240
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni205 – 241RNA-bindingUniRule annotationAdd BLAST37

Sequence similaritiesi

Belongs to the rotavirus NSP2 family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG090000GH

Family and domain databases

Gene3Di3.30.428.20, 1 hit
3.90.1400.10, 1 hit
HAMAPiMF_04089 ROTA_NSP2, 1 hit
InterProiView protein in InterPro
IPR003668 Rotavirus_NSP2
IPR024076 Rotavirus_NSP2_C
IPR024068 Rotavirus_NSP2_N
PfamiView protein in Pfam
PF02509 Rota_NS35, 1 hit
SUPFAMiSSF75347 SSF75347, 1 hit
SSF75574 SSF75574, 1 hit

Sequencei

Sequence statusi: Complete.

Q03240-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELACFCYP HLENDSYKFI PFNNLAIKCM LTAKVDKKDQ DKFYNSIIYG
60 70 80 90 100
IAPPPQFKKR YNTNDNSRGM NYETSMFNKV AALICEALNS TKVTQSDIAS
110 120 130 140 150
VLSRVVSVRH LENLVLRREN HQDVLFHSKE LLLKSVLIAI GHSKEIETTA
160 170 180 190 200
TAEGGEIVFQ NTAFTMWRLT YLEHKLMPIL DPNFIEYKIT VNEGKPISES
210 220 230 240 250
HIKELIAELR WQYNKFAVIT HGKGHYRVVK YSSVANHADR VYATYKSNNK
260 270 280 290 300
NGNMLEFNLL DQRIIWQNWY AFTSSMKQGN TLDTCKKLLF QKIKRESNPF
310
KGLSTDRKMD EVSQIGI
Length:317
Mass (Da):36,611
Last modified:June 1, 1994 - v1
Checksum:iCC105D6E308EC6F0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti194G → D in ABV53255 (PubMed:18786998).1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04529 Genomic RNA Translation: AAA47296.1
EF672580 Genomic RNA Translation: ABV53255.1

Similar proteinsi

Entry informationi

Entry nameiNSP2_ROTHD
AccessioniPrimary (citable) accession number: Q03240
Secondary accession number(s): B3SRT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health