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Q03238 (GRAM_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Granzyme M

EC=3.4.21.-
Alternative name(s):
Met-ase
Natural killer cell granular protease
RNK-Met-1
Gene names
Name:Gzmm
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length258 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha-tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells By similarity.

Subcellular location

Secreted. Cytoplasmic granule. Note: Granules of large granular lymphocytes.

Sequence similarities

Belongs to the peptidase S1 family. Granzyme subfamily.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide‹1 – ? Potential
Propeptide? – 20Activation peptidePRO_0000027423
Chain21 – 258238Granzyme M
PRO_0000027424

Regions

Domain21 – 250230Peptidase S1

Sites

Active site611Charge relay system By similarity
Active site1071Charge relay system By similarity
Active site2041Charge relay system By similarity

Amino acid modifications

Glycosylation1741N-linked (GlcNAc...) Potential
Glycosylation2251N-linked (GlcNAc...) Potential
Disulfide bond46 ↔ 62 By similarity
Disulfide bond142 ↔ 210 By similarity
Disulfide bond173 ↔ 189 By similarity
Disulfide bond200 ↔ 226 By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
Q03238 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: B89DC10EF54DF495

FASTA25828,339
        10         20         30         40         50         60 
LLLLLALKTL WAVGNRFEAQ IIGGREAVPH SRPYMVSLQN TKSHMCGGVL VHQKWVLTAA 

        70         80         90        100        110        120 
HCLSEPLQQL KLVFGLHSLH DPQDPGLTFY IKQAIKHPGY NLKYENDLAL LKLDGRVKPS 

       130        140        150        160        170        180 
KNVKPLALPR KPRDKPAEGS RCSTAGWGIT HQRGQLAKSL QELDLRLLDT RMCNNSRFWN 

       190        200        210        220        230        240 
GVLTDSMLCL KAGAKGQAPC KGDSGGPLVC GKGKVDGILS FSSKNCTDIF KPTVATAVAP 

       250 
YSSWIRKVIG RWSPQPLT 

« Hide

References

[1]"Purification and cloning of a novel serine protease, RNK-Met-1, from the granules of a rat natural killer cell leukemia."
Smyth M.J., Wiltrout T., Trapani J.A., Ottaway K.S., Sowder R., Henderson L.E., Kam C.-M., Powers J.C., Young H.A., Sayers T.J.
J. Biol. Chem. 267:24418-24425(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-44.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L05175 mRNA. Translation: AAA42056.1.
PIRA45161.
UniGeneRn.9838.

3D structure databases

ProteinModelPortalQ03238.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ03238. 1 interaction.
STRING10116.ENSRNOP00000011086.

Protein family/group databases

MEROPSS01.139.

Proteomic databases

PRIDEQ03238.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:620022. rat.

Organism-specific databases

RGD620022. Gzmm.

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidQ03238.
PhylomeDBQ03238.

Gene expression databases

GenevestigatorQ03238.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ03238.

Entry information

Entry nameGRAM_RAT
AccessionPrimary (citable) accession number: Q03238
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 14, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries