Q03238 (GRAM_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Granzyme M
EC=3.4.21.-

Alternative name(s):
Met-ase
Natural killer cell granular protease
RNK-Met-1

Gene names

Name:

Gzmm

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	258 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha-tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells By similarity.
Subcellular location	Secreted. Cytoplasmic granule. Note: Granules of large granular lymphocytes.
Sequence similarities	Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Apoptosis Cytolysis Immunity Innate immunity
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cytolysis Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro serine-type peptidase activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	‹1 – ?		Potential
	Propeptide	? – 20		Activation peptide		PRO_0000027423
	Chain	21 – 258	238	Granzyme M		PRO_0000027424
Regions
	Domain	21 – 250	230	Peptidase S1
Sites
	Active site	61	1	Charge relay system By similarity
	Active site	107	1	Charge relay system By similarity
	Active site	204	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	174	1	N-linked (GlcNAc...) Potential
	Glycosylation	225	1	N-linked (GlcNAc...) Potential
	Disulfide bond	46 ↔ 62		By similarity
	Disulfide bond	142 ↔ 210		By similarity
	Disulfide bond	173 ↔ 189		By similarity
	Disulfide bond	200 ↔ 226		By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q03238 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: B89DC10EF54DF495	FASTA	258	28,339
	10 20 30 40 50 60 LLLLLALKTL WAVGNRFEAQ IIGGREAVPH SRPYMVSLQN TKSHMCGGVL VHQKWVLTAA 70 80 90 100 110 120 HCLSEPLQQL KLVFGLHSLH DPQDPGLTFY IKQAIKHPGY NLKYENDLAL LKLDGRVKPS 130 140 150 160 170 180 KNVKPLALPR KPRDKPAEGS RCSTAGWGIT HQRGQLAKSL QELDLRLLDT RMCNNSRFWN 190 200 210 220 230 240 GVLTDSMLCL KAGAKGQAPC KGDSGGPLVC GKGKVDGILS FSSKNCTDIF KPTVATAVAP 250 YSSWIRKVIG RWSPQPLT

References

[1]

"Purification and cloning of a novel serine protease, RNK-Met-1, from the granules of a rat natural killer cell leukemia." Smyth M.J., Wiltrout T., Trapani J.A., Ottaway K.S., Sowder R., Henderson L.E., Kam C.-M., Powers J.C., Young H.A., Sayers T.J. J. Biol. Chem. 267:24418-24425(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-44.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L05175 mRNA. Translation: AAA42056.1.
IPI	IPI00365967.
PIR	A45161.
UniGene	Rn.9838.
3D structure databases
ProteinModelPortal	Q03238.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q03238. 1 interaction.
STRING	10116.ENSRNOP00000011086.
Protein family/group databases
MEROPS	S01.139.
Proteomic databases
PRIDE	Q03238.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:620022. rat.
Organism-specific databases
RGD	620022. Gzmm.
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	Q03238.
OrthoDB	EOG4TMR2V.
Gene expression databases
Genevestigator	Q03238.
GermOnline	ENSRNOG00000030530. Rattus norvegicus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRAM_RAT

Accession

Primary (citable) accession number: Q03238

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program