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Q03238

- GRAM_RAT

UniProt

Q03238 - GRAM_RAT

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Protein

Granzyme M

Gene

Gzmm

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha-tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei61 – 611Charge relay systemBy similarity
Active sitei107 – 1071Charge relay systemBy similarity
Active sitei204 – 2041Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
  2. serine-type peptidase activity Source: RGD

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. cytolysis Source: UniProtKB-KW
  3. innate immune response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Apoptosis, Cytolysis, Immunity, Innate immunity

Protein family/group databases

MEROPSiS01.139.

Names & Taxonomyi

Protein namesi
Recommended name:
Granzyme M (EC:3.4.21.-)
Alternative name(s):
Met-ase
Natural killer cell granular protease
RNK-Met-1
Gene namesi
Name:Gzmm
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620022. Gzmm.

Subcellular locationi

Secreted. Cytoplasmic granule
Note: Granules of large granular lymphocytes.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei? – 20Activation peptide1 PublicationPRO_0000027423
Signal peptidei‹1 – ?Sequence Analysis
Chaini21 – 258238Granzyme MPRO_0000027424Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi46 ↔ 62PROSITE-ProRule annotation
Disulfide bondi142 ↔ 210PROSITE-ProRule annotation
Disulfide bondi173 ↔ 189PROSITE-ProRule annotation
Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi200 ↔ 226PROSITE-ProRule annotation
Glycosylationi225 – 2251N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiQ03238.

Expressioni

Gene expression databases

GenevestigatoriQ03238.

Interactioni

Protein-protein interaction databases

IntActiQ03238. 1 interaction.
STRINGi10116.ENSRNOP00000011086.

Structurei

3D structure databases

ProteinModelPortaliQ03238.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 250230Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ03238.
PhylomeDBiQ03238.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03238 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
LLLLLALKTL WAVGNRFEAQ IIGGREAVPH SRPYMVSLQN TKSHMCGGVL
60 70 80 90 100
VHQKWVLTAA HCLSEPLQQL KLVFGLHSLH DPQDPGLTFY IKQAIKHPGY
110 120 130 140 150
NLKYENDLAL LKLDGRVKPS KNVKPLALPR KPRDKPAEGS RCSTAGWGIT
160 170 180 190 200
HQRGQLAKSL QELDLRLLDT RMCNNSRFWN GVLTDSMLCL KAGAKGQAPC
210 220 230 240 250
KGDSGGPLVC GKGKVDGILS FSSKNCTDIF KPTVATAVAP YSSWIRKVIG

RWSPQPLT
Length:258
Mass (Da):28,339
Last modified:June 1, 1994 - v1
Checksum:iB89DC10EF54DF495
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L05175 mRNA. Translation: AAA42056.1.
PIRiA45161.
UniGeneiRn.9838.

Genome annotation databases

UCSCiRGD:620022. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L05175 mRNA. Translation: AAA42056.1 .
PIRi A45161.
UniGenei Rn.9838.

3D structure databases

ProteinModelPortali Q03238.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q03238. 1 interaction.
STRINGi 10116.ENSRNOP00000011086.

Protein family/group databases

MEROPSi S01.139.

Proteomic databases

PRIDEi Q03238.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:620022. rat.

Organism-specific databases

RGDi 620022. Gzmm.

Phylogenomic databases

eggNOGi COG5640.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi Q03238.
PhylomeDBi Q03238.

Miscellaneous databases

PROi Q03238.

Gene expression databases

Genevestigatori Q03238.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Purification and cloning of a novel serine protease, RNK-Met-1, from the granules of a rat natural killer cell leukemia."
    Smyth M.J., Wiltrout T., Trapani J.A., Ottaway K.S., Sowder R., Henderson L.E., Kam C.-M., Powers J.C., Young H.A., Sayers T.J.
    J. Biol. Chem. 267:24418-24425(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-44.

Entry informationi

Entry nameiGRAM_RAT
AccessioniPrimary (citable) accession number: Q03238
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3