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Q03238

- GRAM_RAT

UniProt

Q03238 - GRAM_RAT

Protein

Granzyme M

Gene

Gzmm

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    • Comment

    Functioni

    Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha-tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei61 – 611Charge relay systemBy similarity
    Active sitei107 – 1071Charge relay systemBy similarity
    Active sitei204 – 2041Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro
    2. serine-type peptidase activity Source: RGD

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. cytolysis Source: UniProtKB-KW
    3. innate immune response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Apoptosis, Cytolysis, Immunity, Innate immunity

    Protein family/group databases

    MEROPSiS01.139.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Granzyme M (EC:3.4.21.-)
    Alternative name(s):
    Met-ase
    Natural killer cell granular protease
    RNK-Met-1
    Gene namesi
    Name:Gzmm
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620022. Gzmm.

    Subcellular locationi

    Secreted. Cytoplasmic granule
    Note: Granules of large granular lymphocytes.

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei? – 20Activation peptide1 PublicationPRO_0000027423
    Signal peptidei‹1 – ?Sequence Analysis
    Chaini21 – 258238Granzyme MPRO_0000027424Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi46 ↔ 62PROSITE-ProRule annotation
    Disulfide bondi142 ↔ 210PROSITE-ProRule annotation
    Disulfide bondi173 ↔ 189PROSITE-ProRule annotation
    Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi200 ↔ 226PROSITE-ProRule annotation
    Glycosylationi225 – 2251N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PRIDEiQ03238.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03238.

    Interactioni

    Protein-protein interaction databases

    IntActiQ03238. 1 interaction.
    STRINGi10116.ENSRNOP00000011086.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03238.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 250230Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiQ03238.
    PhylomeDBiQ03238.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q03238-1 [UniParc]FASTAAdd to Basket

    « Hide

    LLLLLALKTL WAVGNRFEAQ IIGGREAVPH SRPYMVSLQN TKSHMCGGVL    50
    VHQKWVLTAA HCLSEPLQQL KLVFGLHSLH DPQDPGLTFY IKQAIKHPGY 100
    NLKYENDLAL LKLDGRVKPS KNVKPLALPR KPRDKPAEGS RCSTAGWGIT 150
    HQRGQLAKSL QELDLRLLDT RMCNNSRFWN GVLTDSMLCL KAGAKGQAPC 200
    KGDSGGPLVC GKGKVDGILS FSSKNCTDIF KPTVATAVAP YSSWIRKVIG 250
    RWSPQPLT 258
    Length:258
    Mass (Da):28,339
    Last modified:June 1, 1994 - v1
    Checksum:iB89DC10EF54DF495
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L05175 mRNA. Translation: AAA42056.1.
    PIRiA45161.
    UniGeneiRn.9838.

    Genome annotation databases

    UCSCiRGD:620022. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L05175 mRNA. Translation: AAA42056.1 .
    PIRi A45161.
    UniGenei Rn.9838.

    3D structure databases

    ProteinModelPortali Q03238.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q03238. 1 interaction.
    STRINGi 10116.ENSRNOP00000011086.

    Protein family/group databases

    MEROPSi S01.139.

    Proteomic databases

    PRIDEi Q03238.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:620022. rat.

    Organism-specific databases

    RGDi 620022. Gzmm.

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi Q03238.
    PhylomeDBi Q03238.

    Miscellaneous databases

    PROi Q03238.

    Gene expression databases

    Genevestigatori Q03238.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Purification and cloning of a novel serine protease, RNK-Met-1, from the granules of a rat natural killer cell leukemia."
      Smyth M.J., Wiltrout T., Trapani J.A., Ottaway K.S., Sowder R., Henderson L.E., Kam C.-M., Powers J.C., Young H.A., Sayers T.J.
      J. Biol. Chem. 267:24418-24425(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-44.

    Entry informationi

    Entry nameiGRAM_RAT
    AccessioniPrimary (citable) accession number: Q03238
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3