Reviewed, UniProtKB/Swiss-Prot Q03233 (ADD37_YEAST)

Names and origin

Protein names

Recommended name:
    Alpha1-proteinase inhibitor-degradation deficient protein 37

Gene names

Name:	ADD37
Ordered Locus Names:	YMR184W
ORF Names:	YM8010.14

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in ER-associated protein degradation (ERAD). Ref.3
Subcellular location	Cytoplasm.
Induction	In response to the DNA-damaging agent MMS. Ref.8
Miscellaneous	Present with 1270 molecules/cell in log phase SD medium. Ref.5

Ontologies

Keywords
Cellular component	Cytoplasm
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ER-associated protein catabolic process Ref.3 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Ref.4 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Alpha1-proteinase inhibitor-degradation deficient protein 37		PRO_0000203321
Amino acid modifications
	Modified residue	70	1	Phosphothreonine Ref.9
	Modified residue	79	1	Phosphoserine Ref.6 Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q03233-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 2716B90391A82092	FASTA	198	22,110
	10 20 30 40 50 60 MAIKPTKSFQ NCLEAEVPGY NDCPTVLFSI DPNSGPRSKS KQRTKSKRCV SGRLATEVLD 70 80 90 100 110 120 LYGNTKTATT PPPVLRRPSV TAAQQESACE GVLVKDQGDR QLQPILCSKE ELVAKINDLC 130 140 150 160 170 180 VCGSKLSSKE LEFYKKKLDS NITKILQNEH TKTVLSQIFN EKDKNMAVKT IKHWMVTDTT 190 ISNWCPAFLK IFENAMPN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Differential requirements of novel A1PiZ degradation deficient (ADD) genes in ER-associated protein degradation." Palmer E.A., Kruse K.B., Fewell S.W., Buchanan S.M., Brodsky J.L., McCracken A.A. J. Cell Sci. 116:2361-2373(2003) [PubMed: 12711700] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, MASS SPECTROMETRY.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, MASS SPECTROMETRY.
[8]	"Global protein expression profiling of budding yeast in response to DNA damage." Lee M.-W., Kim B.-J., Choi H.-K., Ryu M.-J., Kim S.-B., Kang K.-M., Cho E.-J., Youn H.-D., Huh W.-K., Kim S.-T. Yeast 24:145-154(2007) [PubMed: 17351896] [Abstract] Cited for: INDUCTION.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-70, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49808 Genomic DNA. Translation: CAA89917.1. AY557970 Genomic DNA. Translation: AAS56296.1.
PIR	S55131.
RefSeq	NP_013909.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3848N.
IntAct	Q03233. 2 interactions.
Proteomic databases
PeptideAtlas	Q03233.
PRIDE	Q03233.
Genome annotation databases
Ensembl	YMR184W; YMR184W; YMR184W; Saccharomyces cerevisiae. [Genome view]
GeneID	855222.
KEGG	sce:YMR184W.
NMPDR	fig|4932.3.peg.4961.
Organism-specific databases
CYGD	YMR184w.
SGD	S000004796. ADD37.
Phylogenomic databases
HOGENOM	Q03233.
OMA	PGYNDCP
OrthoDB	EOG9BZPK8
Gene expression databases
ArrayExpress	Q03233.
Genevestigator	Q03233.
GermOnline	YMR184W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	978744.

Entry information

Entry name

ADD37_YEAST

Accession

Primary (citable) accession number: Q03233

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)