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Q03221

- KITH_BACSU

UniProt

Q03221 - KITH_BACSU

Protein

Thymidine kinase

Gene

tdk

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei89 – 891Proton acceptorUniRule annotation
    Metal bindingi145 – 1451ZincUniRule annotation
    Metal bindingi148 – 1481ZincUniRule annotation
    Metal bindingi183 – 1831ZincUniRule annotation
    Metal bindingi186 – 1861ZincUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi15 – 228ATPUniRule annotation
    Nucleotide bindingi88 – 914ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. thymidine kinase activity Source: UniProtKB-HAMAP
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBSUB:BSU37060-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
    Gene namesi
    Name:tdkUniRule annotation
    Ordered Locus Names:BSU37060
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU37060. [Micado]

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 195195Thymidine kinasePRO_0000174963Add
    BLAST

    Proteomic databases

    PaxDbiQ03221.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224308.BSU37060.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03221.
    SMRiQ03221. Positions 1-191.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidine kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1435.
    HOGENOMiHOG000076390.
    KOiK00857.
    OMAiDHISEST.
    OrthoDBiEOG69D3J2.
    PhylomeDBiQ03221.

    Family and domain databases

    HAMAPiMF_00124. Thymidine_kinase.
    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    IPR020634. Thymidine_kinase_subgr.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03221-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYIMKQSGWL ELICGSMFSG KSEELIRRVK RATYAKQEVR VFKPVIDNRY    50
    SEAAVVSHNG TSMTSYAISS AADIWDHISE STDVVAVDEV QFFDQEIVEV 100
    LSSLADKGYR VIAAGLDMDF RGEPFGVVPN IMAIAESVTK LQAVCSVCGS 150
    PASRTQRLID GKPASYDDPV ILVGAAESYE ARCRHHHEVP GKSKK 195
    Length:195
    Mass (Da):21,450
    Last modified:February 1, 1995 - v2
    Checksum:i1F4EFE266714249F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97678 Unassigned DNA. Translation: AAA02902.1.
    Z49782 Genomic DNA. Translation: CAA89879.1.
    AL009126 Genomic DNA. Translation: CAB15723.1.
    PIRiS55432.
    RefSeqiNP_391587.1. NC_000964.3.
    WP_003243018.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB15723; CAB15723; BSU37060.
    GeneIDi937033.
    KEGGibsu:BSU37060.
    PATRICi18979446. VBIBacSub10457_3885.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97678 Unassigned DNA. Translation: AAA02902.1 .
    Z49782 Genomic DNA. Translation: CAA89879.1 .
    AL009126 Genomic DNA. Translation: CAB15723.1 .
    PIRi S55432.
    RefSeqi NP_391587.1. NC_000964.3.
    WP_003243018.1. NZ_CM000487.1.

    3D structure databases

    ProteinModelPortali Q03221.
    SMRi Q03221. Positions 1-191.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU37060.

    Proteomic databases

    PaxDbi Q03221.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB15723 ; CAB15723 ; BSU37060 .
    GeneIDi 937033.
    KEGGi bsu:BSU37060.
    PATRICi 18979446. VBIBacSub10457_3885.

    Organism-specific databases

    GenoListi BSU37060. [Micado ]

    Phylogenomic databases

    eggNOGi COG1435.
    HOGENOMi HOG000076390.
    KOi K00857.
    OMAi DHISEST.
    OrthoDBi EOG69D3J2.
    PhylomeDBi Q03221.

    Enzyme and pathway databases

    BioCyci BSUB:BSU37060-MONOMER.

    Family and domain databases

    HAMAPi MF_00124. Thymidine_kinase.
    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    IPR020634. Thymidine_kinase_subgr.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF035805. TK_cell. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a putative Bacillus subtilis rho gene."
      Quirk P.G., Dunkley E.A. Jr., Lee P., Krulwich T.A.
      J. Bacteriol. 175:647-654(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BD99 / MS119.
    2. Quirk P.G.
      Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO N-TERMINUS.
    3. "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
      Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
      Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.

    Entry informationi

    Entry nameiKITH_BACSU
    AccessioniPrimary (citable) accession number: Q03221
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3