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Protein

Polynucleotide 5'-triphosphatase

Gene

CTL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in an RNA processing event other than mRNA capping. Releases gamma-phosphate from the 5'-end of RNA to produce a diphosphate terminus.

Catalytic activityi

A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.

Cofactori

Mg2+, Mn2+Note: Magnesium and/or manganese. Specific for polynucleotide RNA in the presence of magnesium, but becomes specific for nucleotide triphosphates in the presence of manganese.

GO - Molecular functioni

  • polynucleotide 5'-phosphatase activity Source: SGD

GO - Biological processi

  • mRNA processing Source: UniProtKB-KW
  • polynucleotide 5' dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

mRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-32868-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polynucleotide 5'-triphosphatase (EC:3.1.3.33)
Alternative name(s):
mRNA 5'-triphosphatase
Short name:
TPase
Gene namesi
Name:CTL1
Ordered Locus Names:YMR180C
ORF Names:YM8010.10C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR180C.
SGDiS000004792. CTL1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Polynucleotide 5'-triphosphatasePRO_0000210120Add
BLAST

Proteomic databases

MaxQBiQ03220.

Interactioni

Protein-protein interaction databases

BioGridi35358. 28 interactions.
DIPiDIP-1776N.
IntActiQ03220. 1 interaction.
MINTiMINT-402953.

Structurei

3D structure databases

ProteinModelPortaliQ03220.
SMRiQ03220. Positions 84-310.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the fungal TPase family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000067072.
KOiK01098.
OrthoDBiEOG7FZ07W.

Family and domain databases

Gene3Di3.20.100.10. 1 hit.
InterProiIPR033469. CYTH-like_dom.
IPR004206. mRNA_triPase_Cet1.
[Graphical view]
PfamiPF02940. mRNA_triPase. 1 hit.
[Graphical view]
SUPFAMiSSF55154. SSF55154. 1 hit.

Sequencei

Sequence statusi: Complete.

Q03220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDQPETPSN SRNSHENVGA KKADANVASK FRSLHISETT KPLTSTRALY
60 70 80 90 100
KTTRNNSRGA TEFHKHVCKL AWKYLACIDK SSISHIEIEM KFGVITDKRT
110 120 130 140 150
HRRMTPHNKP FIVQNRNGRL VSNVPEQMFS SFQELLRSKS ENPSKCAPRV
160 170 180 190 200
VKQVQKYTKD SIYNCNNASK VGKLTSWRCS EDLRNKELKL TYIKKVRVKD
210 220 230 240 250
FLIRYPQSSL DAKISISLEV PEYETSAAFR NGFILQRTKS RSTYTFNDKM
260 270 280 290 300
PLHLDLTKVT TTRRNSHQYT SHEVEVEMDP IFKETISAND REKFNEYMCS
310 320
FLNASDLIRK AAERDNMLTT
Length:320
Mass (Da):36,974
Last modified:November 1, 1997 - v1
Checksum:i82EFBDF3E100B57B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49808 Genomic DNA. Translation: CAA89913.1.
AY693065 Genomic DNA. Translation: AAT93084.1.
BK006946 Genomic DNA. Translation: DAA10077.1.
PIRiS55127.
RefSeqiNP_013905.1. NM_001182686.1.

Genome annotation databases

EnsemblFungiiYMR180C; YMR180C; YMR180C.
GeneIDi855218.
KEGGisce:YMR180C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49808 Genomic DNA. Translation: CAA89913.1.
AY693065 Genomic DNA. Translation: AAT93084.1.
BK006946 Genomic DNA. Translation: DAA10077.1.
PIRiS55127.
RefSeqiNP_013905.1. NM_001182686.1.

3D structure databases

ProteinModelPortaliQ03220.
SMRiQ03220. Positions 84-310.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35358. 28 interactions.
DIPiDIP-1776N.
IntActiQ03220. 1 interaction.
MINTiMINT-402953.

Proteomic databases

MaxQBiQ03220.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR180C; YMR180C; YMR180C.
GeneIDi855218.
KEGGisce:YMR180C.

Organism-specific databases

EuPathDBiFungiDB:YMR180C.
SGDiS000004792. CTL1.

Phylogenomic databases

GeneTreeiENSGT00530000067072.
KOiK01098.
OrthoDBiEOG7FZ07W.

Enzyme and pathway databases

BioCyciYEAST:G3O-32868-MONOMER.

Miscellaneous databases

PROiQ03220.

Family and domain databases

Gene3Di3.20.100.10. 1 hit.
InterProiIPR033469. CYTH-like_dom.
IPR004206. mRNA_triPase_Cet1.
[Graphical view]
PfamiPF02940. mRNA_triPase. 1 hit.
[Graphical view]
SUPFAMiSSF55154. SSF55154. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "A Saccharomyces cerevisiae RNA 5'-triphosphatase related to mRNA capping enzyme."
    Rodriguez C.R., Takagi T., Cho E.J., Buratowski S.
    Nucleic Acids Res. 27:2181-2188(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiCTL1_YEAST
AccessioniPrimary (citable) accession number: Q03220
Secondary accession number(s): D6W003
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 8, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.