Reviewed, UniProtKB/Swiss-Prot Q03220 (CTL1_YEAST)

Names and origin

Protein names

Recommended name:
    Polynucleotide 5'-triphosphatase
    EC=3.1.3.33
Alternative name(s):
    mRNA 5'-triphosphatase
      Short name=TPase

Gene names

Name:	CTL1
Ordered Locus Names:	YMR180C
ORF Names:	YM8010.10C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probably involved in an RNA processing event other than mRNA capping. Releases gamma-phosphate from the 5'-end of RNA to produce a diphosphate terminus.
Catalytic activity	A 5'-phosphopolynucleotide + H(2)O = a polynucleotide + phosphate.
Cofactor	Magnesium and/or manganese. Specific for polynucleotide RNA in the presence of magnesium, but becomes specific for nucleotide triphosphates in the presence of manganese.
Subcellular location	Cytoplasm. Nucleus.
Sequence similarities	Belongs to the fungal TPase family.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Cytoplasm Nucleus
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Ref.3 Inferred from electronic annotation. Source: UniProtKB-KW nucleus Ref.3 Inferred from electronic annotation. Source: InterPro
Molecular function	polynucleotide 5'-phosphatase activity Ref.3 Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Polynucleotide 5'-triphosphatase		PRO_0000210120

Sequences

	Sequence		Length	Mass (Da)
	Q03220-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 82EFBDF3E100B57B	FASTA	320	36,974
	10 20 30 40 50 60 MSDQPETPSN SRNSHENVGA KKADANVASK FRSLHISETT KPLTSTRALY KTTRNNSRGA 70 80 90 100 110 120 TEFHKHVCKL AWKYLACIDK SSISHIEIEM KFGVITDKRT HRRMTPHNKP FIVQNRNGRL 130 140 150 160 170 180 VSNVPEQMFS SFQELLRSKS ENPSKCAPRV VKQVQKYTKD SIYNCNNASK VGKLTSWRCS 190 200 210 220 230 240 EDLRNKELKL TYIKKVRVKD FLIRYPQSSL DAKISISLEV PEYETSAAFR NGFILQRTKS 250 260 270 280 290 300 RSTYTFNDKM PLHLDLTKVT TTRRNSHQYT SHEVEVEMDP IFKETISAND REKFNEYMCS 310 320 FLNASDLIRK AAERDNMLTT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"A Saccharomyces cerevisiae RNA 5'-triphosphatase related to mRNA capping enzyme." Rodriguez C.R., Takagi T., Cho E.J., Buratowski S. Nucleic Acids Res. 27:2181-2188(1999) [PubMed: 10219091] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49808 Genomic DNA. Translation: CAA89913.1. AY693065 Genomic DNA. Translation: AAT93084.1.
PIR	S55127.
RefSeq	NP_013905.1.
3D structure databases
HSSP	HSSP built from PDB template 1D8I based on UniProtKB O13297.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1776N.
IntAct	Q03220.
Genome annotation databases
Ensembl	YMR180C. Saccharomyces cerevisiae. [Contig view]
GeneID	855218.
GenomeReviews	Gene locus YMR180C in contig Z71257_GR.
KEGG	sce:YMR180C.
NMPDR	fig|4932.3.peg.4956.
Organism-specific databases
CYGD	YMR180c.
SGD	S000004792. CTL1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q03220.
Gene expression databases
GermOnline	YMR180C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004206. mRNA_triPase. [Graphical view]
Pfam	PF02940. mRNA_triPase. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	Q03220.
NextBio	978732.

Entry information

Entry name

CTL1_YEAST

Accession

Primary (citable) accession number: Q03220

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)