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Protein

Aliphatic nitrilase

Gene

nitA

Organism
Rhodococcus rhodochrous
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

R-CN + 2 H2O = R-COOH + NH3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei48Proton acceptorPROSITE-ProRule annotation1
Active sitei131Proton donorPROSITE-ProRule annotation1
Active sitei165NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BRENDAi3.5.5.7. 5395.

Names & Taxonomyi

Protein namesi
Recommended name:
Aliphatic nitrilase (EC:3.5.5.7)
Gene namesi
Name:nitA
OrganismiRhodococcus rhodochrous
Taxonomic identifieri1829 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeRhodococcus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi165C → S or A: 100% loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00002040462 – 366Aliphatic nitrilaseAdd BLAST365

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliQ03217.
SMRiQ03217.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 282CN hydrolasePROSITE-ProRule annotationAdd BLAST275

Sequence similaritiesi

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiView protein in InterPro
IPR003010. C-N_Hydrolase.
IPR036526. C-N_Hydrolase_sf.
IPR000132. Nitrilase/CN_hydratase_CS.
PfamiView protein in Pfam
PF00795. CN_hydrolase. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiView protein in PROSITE
PS50263. CN_HYDROLASE. 1 hit.
PS00920. NITRIL_CHT_1. 1 hit.
PS00921. NITRIL_CHT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03217-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEYTNTFKV AAVQAQPVWF DAAKTVDKTV SIIAEAARNG CELVAFPEVF
60 70 80 90 100
IPGYPYHIWV DSPLAGMAKF AVRYHENSLT MDSPHVQRLL DAARDHNIAV
110 120 130 140 150
VVGISERDGG SLYMTQLVID ADGQLVARRR KLKPTHVERS VYGEGNGSDI
160 170 180 190 200
SVYDMPFARL GALNCWEHFQ TLTKYAMYSM HEQVHVASWP GMSLYQPEVP
210 220 230 240 250
AFGVDAQLTA TRMYALEGQT FVVCTTQVVT PEAHEFFCDN DEQRKLIGRG
260 270 280 290 300
GGFARIIGPD GRDLATPLAE DEEGILYADI DLSAITLAKQ AADPVGHYSR
310 320 330 340 350
PDVLSLNFNQ RHTTPVNTAI STIHATHTLV PQSGALDGVR ELNGADEQRA
360
LPSTHSDETD RATASI
Length:366
Mass (Da):40,189
Last modified:January 23, 2007 - v2
Checksum:i3B8E1274AE5EC287
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11425 Genomic DNA. Translation: BAA01994.1.
D67026 Genomic DNA. Translation: BAA11037.1.
PIRiA45070.

Similar proteinsi

Entry informationi

Entry nameiNRL2_RHORH
AccessioniPrimary (citable) accession number: Q03217
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 61 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families