Reviewed, UniProtKB/Swiss-Prot Q03216 (K6PF2_KLULA)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase subunit beta
    EC=2.7.1.11
Alternative name(s):
    Phosphofructokinase 2
    Phosphohexokinase
    6PF-1-K subunit beta

Gene names

Name:	PFK2
Ordered Locus Names:	KLLA0F06248g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	938 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Enzyme regulation	Allosterically inhibited by ATP and activated by AMP and fructose 2,6-bisphosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit structure	Heterooctamer of 4 alpha and 4 beta chains.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 938	938	6-phosphofructokinase subunit beta		PRO_0000112041
Regions
	Nucleotide binding	195 – 199	5	ATP By similarity
	Nucleotide binding	354 – 358	5	ATP By similarity
	Nucleotide binding	371 – 387	17	ATP By similarity
Sites
	Active site	327	1	Proton acceptor By similarity
	Binding site	362	1	Substrate By similarity
	Binding site	454	1	Substrate By similarity
	Binding site	460	1	Substrate By similarity
	Binding site	463	1	Substrate By similarity
Experimental info
	Sequence conflict	523	1	Missing in CAA78964. Ref.1
	Sequence conflict	554	1	D → H in CAA78964. Ref.1
	Sequence conflict	783 – 784	2	FS → LR in CAA78964. Ref.1
	Sequence conflict	927 – 938	12	TIADH…GRKKL → PLRTICRKEKTYEIKNVSAS SKFPLKWMYIIM in CAA78964. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q03216-1 [UniParc]. Last modified September 27, 2004. Version 2. Checksum: CE9C56DD7588C955	FASTA	938	102,477
	10 20 30 40 50 60 MTQSLPLLNG TEAYKLVTTQ GLYDKTVKFY EKYLQLVHDK RVGTLTNSLI TLKLVVDNSF 70 80 90 100 110 120 KPLDVVNDKD WRAIVSSALV FSCTNIQHFR DLAAGETIQA YPNETNPIEI YLKDPNGYII 130 140 150 160 170 180 GITETKNAIS IKPTLPKQSV EASLISSRSS RIDIASSGVS TDSSYPAIPK TAKAQKSIAV 190 200 210 220 230 240 MTSGGDAPGM NANVRAIVRT AIFKGCNAFV VMEGYEGLVK GGPNYIKQVY WETVRNWSCE 250 260 270 280 290 300 GGTNIGTARC KEFREREGRL LGALHLIEAG VDALIVCGGD GSLTGADLFR SEWPSLIREL 310 320 330 340 350 360 LDQGRINKVQ FDRYQHLNIC GTVGSIDNDM STTDATIGAY SALDRICQAI DYIEATANSH 370 380 390 400 410 420 SRAFVVEVMG RNCGWLALLA GISTSADYIL IPEKPASSRE WQDQMCDIIS KHRSRGKRTT 430 440 450 460 470 480 IVIVAEGAIS ADLTPISSKD VHKVLVDRLG LDCRITTLGH VQRGGTAVAY DRILATLQGV 490 500 510 520 530 540 EAVNAVLEST PDTPSPLIAI NENKITRKPL VESVQLTKSV AEAIHSKDFK KAMQLRDSEF 550 560 570 580 590 600 VEHLDNFMAI NSADHIEPKL PEHTHMKIAI VNVGAPAGGM NSAVYSMATY CMSQGHKPYA 610 620 630 640 650 660 IYNGWTGLTR HESVRSLNWK DLLGWQSRGG SEIGTNRHTP EEADIGLIAY YFQKYGFDGI 670 680 690 700 710 720 IIVGGFEAFV SLHQLERARE NYTAFRIPMV LIPATLSNNV PGTEYSLGSD TALNSLMQYC 730 740 750 760 770 780 DIIKQSAAST RGRVFVVDVQ GGNSGYLATH AAVAVGAQVS YVPEEGISLE QLTQDIENLT 790 800 810 820 830 840 ESFSEAEGRG KFGQLILKST NASKVLTPEV LAEVITQEAE GHFDAKCAIP GHVQQGGLPS 850 860 870 880 890 900 PIDRTRGTRF AIRAVGFIES QHKVLAAEAN LDDDDFDFDT PKIIATASVL GVKGSDIVFS 910 920 930 SIRQLYDFET ELNKRTPKTI HWQSTRTIAD HLVGRKKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular genetics of phosphofructokinase in the yeast Kluyveromyces lactis." Heinisch J.J., Kirchrath L., Liesen T., Vogelsang K., Hollenberg C.P. Mol. Microbiol. 8:559-570(1993) [PubMed: 8326866] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z17316 Genomic DNA. Translation: CAA78964.1. CR382126 Genomic DNA. Translation: CAG98071.1.
PIR	S32903.
RefSeq	XP_455363.1.
3D structure databases
SMR	Q03216. Positions 176-522, 519-876, 567-907.
ModBase	Search...
Protein-protein interaction databases
STRING	Q03216.
Genome annotation databases
GeneID	2894998.
GenomeReviews	Gene locus KLLA0F06248g in contig CR382126_GR.
KEGG	kla:KLLA0F06248g.
Phylogenomic databases
eggNOG	fuNOG05452.
HOGENOM	HBG324640.
OMA	ANSHSRA.
OrthoDB	EOG98WDJC.
PhylomeDB	Q03216.
Enzyme and pathway databases
BRENDA	2.7.1.11. 74088.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF2_KLULA

Accession

Primary (citable) accession number: Q03216
Secondary accession number(s): Q6CL26

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	September 27, 2004
Last modified:	February 9, 2010
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)