Reviewed,
UniProtKB/Swiss-Prot Q03216 (K6PF2_KLULA)
Last modified
June 16, 2009.
Version 65.
History...
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: 6-phosphofructokinase subunit beta EC=2.7.1.11 Alternative name(s): Phosphofructokinase 2 Phosphohexokinase 6PF-1-K subunit beta | ||||
| Gene names |
| ||||
| Organism | Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome] | ||||
| Taxonomic identifier | 28985 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces |
Protein attributes
| Sequence length | 938 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. |
| Enzyme regulation | Allosterically inhibited by ATP and activated by AMP and fructose 2,6-bisphosphate. |
| Pathway | |
| Subunit structure | Heterooctamer of 4 alpha and 4 beta chains. |
| Subcellular location | Cytoplasm By similarity. |
| Sequence similarities | Belongs to the phosphofructokinase family. Two domains subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Glycolysis |
| Cellular component | Cytoplasm |
| Domain | Repeat |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Transferase |
| Technical term | Allosteric enzyme Complete proteome |
| Gene Ontology (GO) | |
| Biological process | glycolysis Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | 6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro |
| Molecular function | 6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 938 | 938 | 6-phosphofructokinase subunit beta | PRO_0000112041 | |||||
Regions | |||||||||
| Nucleotide binding | 195 – 199 | 5 | ATP By similarity | ||||||
| Nucleotide binding | 354 – 358 | 5 | ATP By similarity | ||||||
| Nucleotide binding | 371 – 387 | 17 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 327 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 362 | 1 | Substrate By similarity | ||||||
| Binding site | 454 | 1 | Substrate By similarity | ||||||
| Binding site | 460 | 1 | Substrate By similarity | ||||||
| Binding site | 463 | 1 | Substrate By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 523 | 1 | Missing in CAA78964. Ref.1 | ||||||
| Sequence conflict | 554 | 1 | D → H in CAA78964. Ref.1 | ||||||
| Sequence conflict | 783 – 784 | 2 | FS → LR in CAA78964. Ref.1 | ||||||
| Sequence conflict | 927 – 938 | 12 | TIADH…GRKKL → PLRTICRKEKTYEIKNVSAS SKFPLKWMYIIM in CAA78964. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular genetics of phosphofructokinase in the yeast Kluyveromyces lactis." Heinisch J.J., Kirchrath L., Liesen T., Vogelsang K., Hollenberg C.P. Mol. Microbiol. 8:559-570(1993) [PubMed: 8326866] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. |
| [2] | "Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.  Souciet J.-L.Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37. |
Cross-references
Sequence databases | |
|---|---|
| Z17316 Genomic DNA. Translation: CAA78964.1. CR382126 Genomic DNA. Translation: CAG98071.1. | |
| PIR | S32903. |
| RefSeq | XP_455363.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 3PFK based on UniProtKB P00512. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 2894998. |
| KEGG | kla:KLLA0F06248g. |
Phylogenomic databases | |
| HOGENOM | Q03216. |
| OMA | Q03216. ANSHSRA. |
Enzyme and pathway databases | |
| BRENDA | 2.7.1.11. 74088. |
Family and domain databases | |
| InterPro | IPR009161. 6-phosphofructokinase_euk. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view] |
| Pfam | PF00365. PFK. 2 hits. [Graphical view] |
| PIRSF | PIRSF000533. ATP_PFK_euk. 1 hit. |
| PRINTS | PR00476. PHFRCTKINASE. |
| ProDom | PD000707. Ppfruckinase. 2 hits. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR02478. 6PF1K_euk. 1 hit. |
| PROSITE | PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | K6PF2_KLULA | ||||||||
| Accession | Primary (citable) accession number: Q03216 Secondary accession number(s): Q6CL26 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
