6-phosphofructokinase subunit beta - Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast)

Preferred order of sections

Names and origin

Protein names

Recommended name:
6-phosphofructokinase subunit beta
EC=2.7.1.11

Alternative name(s):
6PF-1-K subunit beta
Phosphofructokinase 2
Phosphohexokinase

Gene names

Name:	PFK2
Ordered Locus Names:	KLLA0F06248g

Organism

Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

284590 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces ›

Protein attributes

Sequence length	938 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Enzyme regulation	Allosterically inhibited by ATP and activated by AMP and fructose 2,6-bisphosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit structure	Heterooctamer of 4 alpha and 4 beta chains.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 938

938

6-phosphofructokinase subunit beta

PRO_0000112041

Regions

Nucleotide binding

195 – 199

5

ATP By similarity

Nucleotide binding

354 – 358

5

ATP By similarity

Nucleotide binding

371 – 387

17

ATP By similarity

Sites

Active site

327

1

Proton acceptor By similarity

Binding site

362

1

Substrate By similarity

Binding site

454

1

Substrate By similarity

Binding site

460

1

Substrate By similarity

Binding site

463

1

Substrate By similarity

Experimental info

Sequence conflict

523

1

Missing in CAA78964. Ref.1

Sequence conflict

554

1

D → H in CAA78964. Ref.1

Sequence conflict

783 – 784

2

FS → LR in CAA78964. Ref.1

Sequence conflict

927 – 938

12

TIADH…GRKKL → PLRTICRKEKTYEIKNVSAS SKFPLKWMYIIM in CAA78964. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q03216 [UniParc].

Last modified September 27, 2004. Version 2.
Checksum: CE9C56DD7588C955
Show »

FASTA

938

102,477

        10         20         30         40         50         60 
MTQSLPLLNG TEAYKLVTTQ GLYDKTVKFY EKYLQLVHDK RVGTLTNSLI TLKLVVDNSF 

        70         80         90        100        110        120 
KPLDVVNDKD WRAIVSSALV FSCTNIQHFR DLAAGETIQA YPNETNPIEI YLKDPNGYII 

       130        140        150        160        170        180 
GITETKNAIS IKPTLPKQSV EASLISSRSS RIDIASSGVS TDSSYPAIPK TAKAQKSIAV 

       190        200        210        220        230        240 
MTSGGDAPGM NANVRAIVRT AIFKGCNAFV VMEGYEGLVK GGPNYIKQVY WETVRNWSCE 

       250        260        270        280        290        300 
GGTNIGTARC KEFREREGRL LGALHLIEAG VDALIVCGGD GSLTGADLFR SEWPSLIREL 

       310        320        330        340        350        360 
LDQGRINKVQ FDRYQHLNIC GTVGSIDNDM STTDATIGAY SALDRICQAI DYIEATANSH 

       370        380        390        400        410        420 
SRAFVVEVMG RNCGWLALLA GISTSADYIL IPEKPASSRE WQDQMCDIIS KHRSRGKRTT 

       430        440        450        460        470        480 
IVIVAEGAIS ADLTPISSKD VHKVLVDRLG LDCRITTLGH VQRGGTAVAY DRILATLQGV 

       490        500        510        520        530        540 
EAVNAVLEST PDTPSPLIAI NENKITRKPL VESVQLTKSV AEAIHSKDFK KAMQLRDSEF 

       550        560        570        580        590        600 
VEHLDNFMAI NSADHIEPKL PEHTHMKIAI VNVGAPAGGM NSAVYSMATY CMSQGHKPYA 

       610        620        630        640        650        660 
IYNGWTGLTR HESVRSLNWK DLLGWQSRGG SEIGTNRHTP EEADIGLIAY YFQKYGFDGI 

       670        680        690        700        710        720 
IIVGGFEAFV SLHQLERARE NYTAFRIPMV LIPATLSNNV PGTEYSLGSD TALNSLMQYC 

       730        740        750        760        770        780 
DIIKQSAAST RGRVFVVDVQ GGNSGYLATH AAVAVGAQVS YVPEEGISLE QLTQDIENLT 

       790        800        810        820        830        840 
ESFSEAEGRG KFGQLILKST NASKVLTPEV LAEVITQEAE GHFDAKCAIP GHVQQGGLPS 

       850        860        870        880        890        900 
PIDRTRGTRF AIRAVGFIES QHKVLAAEAN LDDDDFDFDT PKIIATASVL GVKGSDIVFS 

       910        920        930 
SIRQLYDFET ELNKRTPKTI HWQSTRTIAD HLVGRKKL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular genetics of phosphofructokinase in the yeast Kluyveromyces lactis." Heinisch J.J., Kirchrath L., Liesen T., Vogelsang K., Hollenberg C.P. Mol. Microbiol. 8:559-570(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. Souciet J.-L. Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z17316 Genomic DNA. Translation: CAA78964.1. CR382126 Genomic DNA. Translation: CAG98071.1.
PIR	S32903.
RefSeq	XP_455363.1. XM_455363.1.
3D structure databases
ProteinModelPortal	Q03216.
ModBase	Search...
Protein-protein interaction databases
STRING	28985.Q03216.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2894998.
KEGG	kla:KLLA0F06248g.
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000200154.
KO	K00850.
OMA	QQGGLPS.
OrthoDB	EOG418FWH.
Enzyme and pathway databases
SABIO-RK	Q03216.
UniPathway	UPA00109; UER00182.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 2 hits.
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF2_KLULA

Accession

Primary (citable) accession number: Q03216
Secondary accession number(s): Q6CL26

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	September 27, 2004
Last modified:	May 1, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents