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Q03216

- PFKA2_KLULA

UniProt

Q03216 - PFKA2_KLULA

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Protein

ATP-dependent 6-phosphofructokinase subunit beta

Gene

PFK2

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei185 – 1851ATP; via amide nitrogenUniRule annotation
Metal bindingi280 – 2801Magnesium; catalyticUniRule annotation
Active sitei327 – 3271Proton acceptorUniRule annotation
Binding sitei362 – 3621Substrate; shared with subunit alphaUniRule annotation
Binding sitei426 – 4261SubstrateUniRule annotation
Binding sitei454 – 4541Substrate; shared with subunit alphaUniRule annotation
Binding sitei637 – 6371Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei733 – 7331Allosteric activator fructose 2,6-bisphosphate; shared with subunit alphaUniRule annotation
Binding sitei826 – 8261Allosteric activator fructose 2,6-bisphosphate; shared with subunit alphaUniRule annotation
Binding sitei915 – 9151Allosteric activator fructose 2,6-bisphosphateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi249 – 2502ATPUniRule annotation
Nucleotide bindingi279 – 2824ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKQ03216.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase subunit betaUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 2UniRule annotation
Short name:
Phosphofructokinase 2UniRule annotation
Alternative name(s):
Phosphohexokinase 2UniRule annotation
Gene namesi
Name:PFK2
Ordered Locus Names:KLLA0F06248g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598: Chromosome F

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 938938ATP-dependent 6-phosphofructokinase subunit betaPRO_0000112041Add
BLAST

Interactioni

Subunit structurei

Heterooctamer of 4 alpha and 4 beta chains.

Protein-protein interaction databases

STRINGi28985.Q03216.

Structurei

3D structure databases

ProteinModelPortaliQ03216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 552552N-terminal catalytic PFK domain 1Add
BLAST
Regioni325 – 3273Substrate bindingUniRule annotation
Regioni369 – 3713Substrate bindingUniRule annotation
Regioni460 – 4634Substrate bindingUniRule annotation
Regioni553 – 56614Interdomain linkerAdd
BLAST
Regioni567 – 938372C-terminal regulatory PFK domain 2Add
BLAST
Regioni695 – 6995Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni740 – 7423Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni832 – 8354Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000200154.
InParanoidiQ03216.
KOiK00850.
OMAiHKPYAIY.
OrthoDBiEOG7Q5HPV.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03216-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQSLPLLNG TEAYKLVTTQ GLYDKTVKFY EKYLQLVHDK RVGTLTNSLI
60 70 80 90 100
TLKLVVDNSF KPLDVVNDKD WRAIVSSALV FSCTNIQHFR DLAAGETIQA
110 120 130 140 150
YPNETNPIEI YLKDPNGYII GITETKNAIS IKPTLPKQSV EASLISSRSS
160 170 180 190 200
RIDIASSGVS TDSSYPAIPK TAKAQKSIAV MTSGGDAPGM NANVRAIVRT
210 220 230 240 250
AIFKGCNAFV VMEGYEGLVK GGPNYIKQVY WETVRNWSCE GGTNIGTARC
260 270 280 290 300
KEFREREGRL LGALHLIEAG VDALIVCGGD GSLTGADLFR SEWPSLIREL
310 320 330 340 350
LDQGRINKVQ FDRYQHLNIC GTVGSIDNDM STTDATIGAY SALDRICQAI
360 370 380 390 400
DYIEATANSH SRAFVVEVMG RNCGWLALLA GISTSADYIL IPEKPASSRE
410 420 430 440 450
WQDQMCDIIS KHRSRGKRTT IVIVAEGAIS ADLTPISSKD VHKVLVDRLG
460 470 480 490 500
LDCRITTLGH VQRGGTAVAY DRILATLQGV EAVNAVLEST PDTPSPLIAI
510 520 530 540 550
NENKITRKPL VESVQLTKSV AEAIHSKDFK KAMQLRDSEF VEHLDNFMAI
560 570 580 590 600
NSADHIEPKL PEHTHMKIAI VNVGAPAGGM NSAVYSMATY CMSQGHKPYA
610 620 630 640 650
IYNGWTGLTR HESVRSLNWK DLLGWQSRGG SEIGTNRHTP EEADIGLIAY
660 670 680 690 700
YFQKYGFDGI IIVGGFEAFV SLHQLERARE NYTAFRIPMV LIPATLSNNV
710 720 730 740 750
PGTEYSLGSD TALNSLMQYC DIIKQSAAST RGRVFVVDVQ GGNSGYLATH
760 770 780 790 800
AAVAVGAQVS YVPEEGISLE QLTQDIENLT ESFSEAEGRG KFGQLILKST
810 820 830 840 850
NASKVLTPEV LAEVITQEAE GHFDAKCAIP GHVQQGGLPS PIDRTRGTRF
860 870 880 890 900
AIRAVGFIES QHKVLAAEAN LDDDDFDFDT PKIIATASVL GVKGSDIVFS
910 920 930
SIRQLYDFET ELNKRTPKTI HWQSTRTIAD HLVGRKKL
Length:938
Mass (Da):102,477
Last modified:September 27, 2004 - v2
Checksum:iCE9C56DD7588C955
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti523 – 5231Missing in CAA78964. (PubMed:8326866)Curated
Sequence conflicti554 – 5541D → H in CAA78964. (PubMed:8326866)Curated
Sequence conflicti783 – 7842FS → LR in CAA78964. (PubMed:8326866)Curated
Sequence conflicti927 – 93812TIADH…GRKKL → PLRTICRKEKTYEIKNVSAS SKFPLKWMYIIM in CAA78964. (PubMed:8326866)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z17316 Genomic DNA. Translation: CAA78964.1.
CR382126 Genomic DNA. Translation: CAG98071.1.
PIRiS32903.
RefSeqiXP_455363.1. XM_455363.1.

Genome annotation databases

GeneIDi2894998.
KEGGikla:KLLA0F06248g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z17316 Genomic DNA. Translation: CAA78964.1 .
CR382126 Genomic DNA. Translation: CAG98071.1 .
PIRi S32903.
RefSeqi XP_455363.1. XM_455363.1.

3D structure databases

ProteinModelPortali Q03216.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 28985.Q03216.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2894998.
KEGGi kla:KLLA0F06248g.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000200154.
InParanoidi Q03216.
KOi K00850.
OMAi HKPYAIY.
OrthoDBi EOG7Q5HPV.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
SABIO-RK Q03216.

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular genetics of phosphofructokinase in the yeast Kluyveromyces lactis."
    Heinisch J.J., Kirchrath L., Liesen T., Vogelsang K., Hollenberg C.P.
    Mol. Microbiol. 8:559-570(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Entry informationi

Entry nameiPFKA2_KLULA
AccessioniPrimary (citable) accession number: Q03216
Secondary accession number(s): Q6CL26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: September 27, 2004
Last modified: October 29, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3