Q03216 (K6PF2_KLULA) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 85.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: 6-phosphofructokinase subunit beta EC=2.7.1.11 Alternative name(s): 6PF-1-K subunit beta Phosphofructokinase 2 Phosphohexokinase | ||||
| Gene names |
| ||||
| Organism | Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome] | ||||
| Taxonomic identifier | 284590 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces |
Protein attributes
| Sequence length | 938 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Catalytic activity | ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. |
| Enzyme regulation | Allosterically inhibited by ATP and activated by AMP and fructose 2,6-bisphosphate. |
| Pathway | |
| Subunit structure | Heterooctamer of 4 alpha and 4 beta chains. |
| Subcellular location | Cytoplasm By similarity. |
| Sequence similarities | Belongs to the phosphofructokinase family. Two domains subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Glycolysis |
| Cellular component | Cytoplasm |
| Domain | Repeat |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Transferase |
| Technical term | Allosteric enzyme Complete proteome |
| Gene Ontology (GO) | |
| Biological process | glycolysis Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | 6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro |
| Molecular function | 6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 938 | 938 | 6-phosphofructokinase subunit beta | PRO_0000112041 | |||||
Regions | |||||||||
| Nucleotide binding | 195 – 199 | 5 | ATP By similarity | ||||||
| Nucleotide binding | 354 – 358 | 5 | ATP By similarity | ||||||
| Nucleotide binding | 371 – 387 | 17 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 327 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 362 | 1 | Substrate By similarity | ||||||
| Binding site | 454 | 1 | Substrate By similarity | ||||||
| Binding site | 460 | 1 | Substrate By similarity | ||||||
| Binding site | 463 | 1 | Substrate By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 523 | 1 | Missing in CAA78964. Ref.1 | ||||||
| Sequence conflict | 554 | 1 | D → H in CAA78964. Ref.1 | ||||||
| Sequence conflict | 783 – 784 | 2 | FS → LR in CAA78964. Ref.1 | ||||||
| Sequence conflict | 927 – 938 | 12 | TIADH…GRKKL → PLRTICRKEKTYEIKNVSAS SKFPLKWMYIIM in CAA78964. Ref.1 | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Molecular genetics of phosphofructokinase in the yeast Kluyveromyces lactis." Heinisch J.J., Kirchrath L., Liesen T., Vogelsang K., Hollenberg C.P. Mol. Microbiol. 8:559-570(1993) [PubMed: 8326866] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. |
| [2] | "Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.  Souciet J.-L.Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Z17316 Genomic DNA. Translation: CAA78964.1. CR382126 Genomic DNA. Translation: CAG98071.1. |
| PIR | S32903. |
| RefSeq | XP_455363.1. XM_455363.1. |
3D structure databases | |
| ProteinModelPortal | Q03216. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q03216. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 2894998. |
| GenomeReviews | Gene locus KLLA0F06248g in contig CR382126_GR. |
| KEGG | kla:KLLA0F06248g. |
Phylogenomic databases | |
| eggNOG | fuNOG05452. |
| HOGENOM | HBG324640. |
| OMA | HNGFAGL. |
| OrthoDB | EOG418FWH. |
Family and domain databases | |
| InterPro | IPR009161. 6-phosphofructokinase_euk. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view] |
| KO | K00850. |
| Pfam | PF00365. PFK. 2 hits. [Graphical view] |
| PIRSF | PIRSF000533. ATP_PFK_euk. 1 hit. |
| PRINTS | PR00476. PHFRCTKINASE. |
| SUPFAM | SSF53784. Ppfruckinase. 2 hits. |
| TIGRFAMs | TIGR02478. 6PF1K_euk. 1 hit. |
| PROSITE | PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | K6PF2_KLULA | ||||||||
| Accession | Primary (citable) accession number: Q03216 Secondary accession number(s): Q6CL26 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |