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Q03215

- PFKA1_KLULA

UniProt

Q03215 - PFKA1_KLULA

Protein

ATP-dependent 6-phosphofructokinase subunit alpha

Gene

PFK1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei193 – 1931ATP; via amide nitrogenUniRule annotation
    Metal bindingi287 – 2871Magnesium; catalyticUniRule annotation
    Active sitei334 – 3341Proton acceptorUniRule annotation
    Binding sitei369 – 3691Substrate; shared with subunit betaUniRule annotation
    Binding sitei433 – 4331SubstrateUniRule annotation
    Binding sitei460 – 4601Substrate; shared with subunit betaUniRule annotation
    Binding sitei643 – 6431Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei738 – 7381Allosteric activator fructose 2,6-bisphosphate; shared with subunit betaUniRule annotation
    Binding sitei805 – 8051Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei831 – 8311Allosteric activator fructose 2,6-bisphosphate; shared with subunit betaUniRule annotation
    Binding sitei929 – 9291Allosteric activator fructose 2,6-bisphosphateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi256 – 2572ATPUniRule annotation
    Nucleotide bindingi286 – 2894ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    SABIO-RKQ03215.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase subunit alphaUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFK 1UniRule annotation
    Short name:
    Phosphofructokinase 1UniRule annotation
    Alternative name(s):
    Phosphohexokinase 1UniRule annotation
    Gene namesi
    Name:PFK1
    Ordered Locus Names:KLLA0A05544g
    OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
    Taxonomic identifieri284590 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
    ProteomesiUP000000598: Chromosome A

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 992992ATP-dependent 6-phosphofructokinase subunit alphaPRO_0000112040Add
    BLAST

    Interactioni

    Subunit structurei

    Heterooctamer of 4 alpha and 4 beta chains.

    Protein-protein interaction databases

    STRINGi28985.Q03215.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03215.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 558558N-terminal catalytic PFK domain 1Add
    BLAST
    Regioni332 – 3343Substrate bindingUniRule annotation
    Regioni376 – 3783Substrate bindingUniRule annotation
    Regioni466 – 4694Substrate bindingUniRule annotation
    Regioni559 – 57214Interdomain linkerAdd
    BLAST
    Regioni573 – 992420C-terminal regulatory PFK domain 2Add
    BLAST
    Regioni700 – 7045Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni745 – 7473Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni837 – 8404Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000200154.
    KOiK00850.
    OMAiSAKAMQW.
    OrthoDBiEOG7Q5HPV.

    Family and domain databases

    HAMAPiMF_03184. Phosphofructokinase_I_E.
    InterProiIPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 3 hits.
    TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03215-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNSVYGVAF RSLSTGDEKL YKEATRFYHS LGFQTVKLYD NFKNHDDSNL    50
    IVGTSKNSVK ECWLESFKLS ELDSQGFRVP QQEASNQLQT DGVMIKLRLV 100
    DTEPLNQTAD TVVYYTVSLD EVSKIGERVD DHNVKLVDPL GNVVLVTDSH 150
    DGKELNASEF IAPKDSSVEQ KITVELVDKD SKKKKIAVMT SGGDSQGMNA 200
    AVRAVVRSSI YYGCDVYAVY EGYEGLVKGG DYLRKMEWKD VRGWLSEGGT 250
    LIGTARSKEF RERWGRKQAC SNLIDQGIDA LVVIGGDGSL TGADLFRSEW 300
    PSLVEELVKD GKFTEDEVAL YQNLTIVGMV GSIDNDMSGT DSTIGAYSAL 350
    ERICEMVDYI DATAKSHSRA FVVEVMGRHC GWLGLMSGIA TAADYIFIPE 400
    RAAPHGKWQD ELKRVCQRHR EKGRRNNTVI VAEGALDDQL NPITAEQVKD 450
    VLVELGLDTK ITTLGHVQRG GTAVAHDRWL ATLQGVDAVK AILNMTPETP 500
    SPLIGILENK VIRMPLVESV KLTKQVAAAI EAKDFDKAIS LRDTEFIELY 550
    SNFMSTTVND DGSQLLPEAD RLNIAIVHVG APSAALNAAT RAATLYCLAH 600
    GHRPYAITNG FSGLIQTGQV KELSWIDVED WHNLGGSEIG TNRSVAAEDM 650
    GTIAYHFQKN KFDGVIILGG FEGFKSLKQL RDGRDQYPIF NIPMCLIPAT 700
    VSNNVPGTEY SLGSDTCLNA LVKYTDAIKQ SASSTRRRVF VVEVQGGHSG 750
    YVASFTGLVT GAVSVYTPEN AINLKTIQED LALLKESFKH EQGETRNGKL 800
    VIRNEMASDV YTTELLADII TEQSNDRFGV RTAIPGHVQQ GGVPSSKDRV 850
    IASRFAVKCV KFIEQWNKKN TAADNEDFKI LRFNYVNGVK QYTVLDEDLS 900
    AAVICVNGSK ISFKPIAHIW ENETNIELRK GQEIHWEEYN EIGDILSGRS 950
    MLRRKIQKEQ QEESSLPSVA DTPLSSVTVS TSAAKEDSAL YV 992
    Length:992
    Mass (Da):109,336
    Last modified:October 1, 1993 - v1
    Checksum:i724687F850F27F79
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z17315 Genomic DNA. Translation: CAA78963.1.
    CR382121 Genomic DNA. Translation: CAH02833.1.
    PIRiS32902.
    RefSeqiXP_451245.1. XM_451245.1.

    Genome annotation databases

    GeneIDi2896656.
    KEGGikla:KLLA0A05544g.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z17315 Genomic DNA. Translation: CAA78963.1 .
    CR382121 Genomic DNA. Translation: CAH02833.1 .
    PIRi S32902.
    RefSeqi XP_451245.1. XM_451245.1.

    3D structure databases

    ProteinModelPortali Q03215.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 28985.Q03215.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2896656.
    KEGGi kla:KLLA0A05544g.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000200154.
    KOi K00850.
    OMAi SAKAMQW.
    OrthoDBi EOG7Q5HPV.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    SABIO-RK Q03215.

    Family and domain databases

    HAMAPi MF_03184. Phosphofructokinase_I_E.
    InterProi IPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 3 hits.
    TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular genetics of phosphofructokinase in the yeast Kluyveromyces lactis."
      Heinisch J.J., Kirchrath L., Liesen T., Vogelsang K., Hollenberg C.P.
      Mol. Microbiol. 8:559-570(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    2. "Genome evolution in yeasts."
      Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
      , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
      Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

    Entry informationi

    Entry nameiPFKA1_KLULA
    AccessioniPrimary (citable) accession number: Q03215
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3