Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q03215

- PFKA1_KLULA

UniProt

Q03215 - PFKA1_KLULA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

ATP-dependent 6-phosphofructokinase subunit alpha

Gene

PFK1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei193 – 1931ATP; via amide nitrogenUniRule annotation
Metal bindingi287 – 2871Magnesium; catalyticUniRule annotation
Active sitei334 – 3341Proton acceptorUniRule annotation
Binding sitei369 – 3691Substrate; shared with subunit betaUniRule annotation
Binding sitei433 – 4331SubstrateUniRule annotation
Binding sitei460 – 4601Substrate; shared with subunit betaUniRule annotation
Binding sitei643 – 6431Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei738 – 7381Allosteric activator fructose 2,6-bisphosphate; shared with subunit betaUniRule annotation
Binding sitei805 – 8051Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei831 – 8311Allosteric activator fructose 2,6-bisphosphate; shared with subunit betaUniRule annotation
Binding sitei929 – 9291Allosteric activator fructose 2,6-bisphosphateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi256 – 2572ATPUniRule annotation
Nucleotide bindingi286 – 2894ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKQ03215.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase subunit alphaUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 1UniRule annotation
Short name:
Phosphofructokinase 1UniRule annotation
Alternative name(s):
Phosphohexokinase 1UniRule annotation
Gene namesi
Name:PFK1
Ordered Locus Names:KLLA0A05544g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
ProteomesiUP000000598: Chromosome A

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 992992ATP-dependent 6-phosphofructokinase subunit alphaPRO_0000112040Add
BLAST

Interactioni

Subunit structurei

Heterooctamer of 4 alpha and 4 beta chains.

Protein-protein interaction databases

STRINGi28985.Q03215.

Structurei

3D structure databases

ProteinModelPortaliQ03215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 558558N-terminal catalytic PFK domain 1Add
BLAST
Regioni332 – 3343Substrate bindingUniRule annotation
Regioni376 – 3783Substrate bindingUniRule annotation
Regioni466 – 4694Substrate bindingUniRule annotation
Regioni559 – 57214Interdomain linkerAdd
BLAST
Regioni573 – 992420C-terminal regulatory PFK domain 2Add
BLAST
Regioni700 – 7045Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni745 – 7473Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni837 – 8404Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000200154.
InParanoidiQ03215.
KOiK00850.
OMAiSAKAMQW.
OrthoDBiEOG7Q5HPV.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 3 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03215-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNNSVYGVAF RSLSTGDEKL YKEATRFYHS LGFQTVKLYD NFKNHDDSNL
60 70 80 90 100
IVGTSKNSVK ECWLESFKLS ELDSQGFRVP QQEASNQLQT DGVMIKLRLV
110 120 130 140 150
DTEPLNQTAD TVVYYTVSLD EVSKIGERVD DHNVKLVDPL GNVVLVTDSH
160 170 180 190 200
DGKELNASEF IAPKDSSVEQ KITVELVDKD SKKKKIAVMT SGGDSQGMNA
210 220 230 240 250
AVRAVVRSSI YYGCDVYAVY EGYEGLVKGG DYLRKMEWKD VRGWLSEGGT
260 270 280 290 300
LIGTARSKEF RERWGRKQAC SNLIDQGIDA LVVIGGDGSL TGADLFRSEW
310 320 330 340 350
PSLVEELVKD GKFTEDEVAL YQNLTIVGMV GSIDNDMSGT DSTIGAYSAL
360 370 380 390 400
ERICEMVDYI DATAKSHSRA FVVEVMGRHC GWLGLMSGIA TAADYIFIPE
410 420 430 440 450
RAAPHGKWQD ELKRVCQRHR EKGRRNNTVI VAEGALDDQL NPITAEQVKD
460 470 480 490 500
VLVELGLDTK ITTLGHVQRG GTAVAHDRWL ATLQGVDAVK AILNMTPETP
510 520 530 540 550
SPLIGILENK VIRMPLVESV KLTKQVAAAI EAKDFDKAIS LRDTEFIELY
560 570 580 590 600
SNFMSTTVND DGSQLLPEAD RLNIAIVHVG APSAALNAAT RAATLYCLAH
610 620 630 640 650
GHRPYAITNG FSGLIQTGQV KELSWIDVED WHNLGGSEIG TNRSVAAEDM
660 670 680 690 700
GTIAYHFQKN KFDGVIILGG FEGFKSLKQL RDGRDQYPIF NIPMCLIPAT
710 720 730 740 750
VSNNVPGTEY SLGSDTCLNA LVKYTDAIKQ SASSTRRRVF VVEVQGGHSG
760 770 780 790 800
YVASFTGLVT GAVSVYTPEN AINLKTIQED LALLKESFKH EQGETRNGKL
810 820 830 840 850
VIRNEMASDV YTTELLADII TEQSNDRFGV RTAIPGHVQQ GGVPSSKDRV
860 870 880 890 900
IASRFAVKCV KFIEQWNKKN TAADNEDFKI LRFNYVNGVK QYTVLDEDLS
910 920 930 940 950
AAVICVNGSK ISFKPIAHIW ENETNIELRK GQEIHWEEYN EIGDILSGRS
960 970 980 990
MLRRKIQKEQ QEESSLPSVA DTPLSSVTVS TSAAKEDSAL YV
Length:992
Mass (Da):109,336
Last modified:October 1, 1993 - v1
Checksum:i724687F850F27F79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z17315 Genomic DNA. Translation: CAA78963.1.
CR382121 Genomic DNA. Translation: CAH02833.1.
PIRiS32902.
RefSeqiXP_451245.1. XM_451245.1.

Genome annotation databases

GeneIDi2896656.
KEGGikla:KLLA0A05544g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z17315 Genomic DNA. Translation: CAA78963.1 .
CR382121 Genomic DNA. Translation: CAH02833.1 .
PIRi S32902.
RefSeqi XP_451245.1. XM_451245.1.

3D structure databases

ProteinModelPortali Q03215.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 28985.Q03215.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2896656.
KEGGi kla:KLLA0A05544g.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000200154.
InParanoidi Q03215.
KOi K00850.
OMAi SAKAMQW.
OrthoDBi EOG7Q5HPV.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
SABIO-RK Q03215.

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 3 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular genetics of phosphofructokinase in the yeast Kluyveromyces lactis."
    Heinisch J.J., Kirchrath L., Liesen T., Vogelsang K., Hollenberg C.P.
    Mol. Microbiol. 8:559-570(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Entry informationi

Entry nameiPFKA1_KLULA
AccessioniPrimary (citable) accession number: Q03215
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 26, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3