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Protein

High-osmolarity-induced transcription protein 1

Gene

HOT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for a complete transcriptional response to osmotic stress, through recruitment of HOG1 followed by pol II recruitment to the promoters of GPD1 and other HOG-dependent genes.4 Publications

Miscellaneous

Present with 149 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32860-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
High-osmolarity-induced transcription protein 1
Gene namesi
Name:HOT1
Ordered Locus Names:YMR172W
ORF Names:YM8010.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR172W
SGDiS000004783 HOT1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi30S → A: Impairs HOT1 phosphorylation; when associated with A-70; A-153; A-360 and A-410. 1 Publication1
Mutagenesisi70S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-153; A-360 and A-410. 1 Publication1
Mutagenesisi153S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-360 and A-410. 1 Publication1
Mutagenesisi360S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-153 and A-410. 1 Publication1
Mutagenesisi410S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-153 and A-360. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002033171 – 719High-osmolarity-induced transcription protein 1Add BLAST719

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei146PhosphoserineCombined sources1
Modified residuei153PhosphoserineCombined sources1

Post-translational modificationi

Hyperphosphorylated during acute stress.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03213
PaxDbiQ03213
PRIDEiQ03213
TopDownProteomicsiQ03213

PTM databases

iPTMnetiQ03213

Interactioni

Subunit structurei

Interacts with HOG1.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35350, 82 interactors
DIPiDIP-2367N
IntActiQ03213, 8 interactors
MINTiQ03213
STRINGi4932.YMR172W

Structurei

3D structure databases

ProteinModelPortaliQ03213
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HOT1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000068684
InParanoidiQ03213
KOiK19815
OMAiTIGWLQQ
OrthoDBiEOG092C25VG

Family and domain databases

InterProiView protein in InterPro
IPR022210 TF_GCR1-like
PfamiView protein in Pfam
PF12550 GCR1_C, 1 hit

Sequencei

Sequence statusi: Complete.

Q03213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGMGIAILC IVRTKIYRIT ISFDYSTLMS PFFLFLMMPT TLKDGYRMNS
60 70 80 90 100
QVNEDAIGIN LDLSLPTHIS PTTGSESASG SNASTLRNDG NALDGGLLRT
110 120 130 140 150
SAAISAPTGT SQPTETIGEK LSNEERVNSN VSASNSTTAG TGRMLSQSLT
160 170 180 190 200
NDSPSNEIST DQLKIFQRMD EMSARMIEME ESFNKLSNKI AEQNTMVLNL
210 220 230 240 250
KQDNYKVMNK LNILLKLVAQ PSARPSTNNA QNKLAIELLN SISAVSSAYL
260 270 280 290 300
QKMQNNGSGR QHTADLCTGD SNTHSGINQH RTTNGTIDVN TNTAQLNNQF
310 320 330 340 350
SNALNTILPD QQHNRNNVSQ NINQSLPNRQ LGPVINTQAN QNQSQVLIHN
360 370 380 390 400
TNTHQQVNRS PISFPNASTD KPFKLNPNGI KRRRRNTQSN NNASTNDHAS
410 420 430 440 450
AAQKPISALS PLTNSHNSTT SMNYTNSSIH SGVTSASNSF HDLNSLNNFG
460 470 480 490 500
TTTALSLPSL ALDNASFPPN QNVIPPIINN TQQPLSFSQL INQDSTTSEL
510 520 530 540 550
LPSGKSGVNT NIVNRNRAST LPSYPKPMTV KSNVDDDGYQ EDDDDDGDDE
560 570 580 590 600
GDGRDNEEDS TAEEDEVDDE IETDMKNASI NKRRRSLHHK KSNSLNGRRK
610 620 630 640 650
LHGESATKPN INSDLHYRIL KAPTDVKTIW EEYDTGIRGK PSIKHLEAKY
660 670 680 690 700
GNKWRLNKNK KTFSRRKRLY KFILNGMERG KTAQEMIETL ENKRLYKDDE
710
DGEVKKRTIG WLQESLAGI
Length:719
Mass (Da):79,416
Last modified:November 1, 1997 - v1
Checksum:i4652BE93743D5A54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49808 Genomic DNA Translation: CAA89905.1
BK006946 Genomic DNA Translation: DAA10068.1
PIRiS55119
RefSeqiNP_013895.1, NM_001182677.1

Genome annotation databases

EnsemblFungiiYMR172W; YMR172W; YMR172W
GeneIDi855208
KEGGisce:YMR172W

Similar proteinsi

Entry informationi

Entry nameiHOT1_YEAST
AccessioniPrimary (citable) accession number: Q03213
Secondary accession number(s): D6VZZ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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