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Q03213

- HOT1_YEAST

UniProt

Q03213 - HOT1_YEAST

Protein

High-osmolarity-induced transcription protein 1

Gene

HOT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Required for a complete transcriptional response to osmotic stress, through recruitment of HOG1 followed by pol II recruitment to the promoters of GPD1 and other HOG-dependent genes.4 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly Source: SGD
    3. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: SGD

    GO - Biological processi

    1. cellular hyperosmotic salinity response Source: SGD
    2. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    3. positive regulation of transcription from RNA polymerase II promoter in response to osmotic stress Source: SGD
    4. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32860-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    High-osmolarity-induced transcription protein 1
    Gene namesi
    Name:HOT1
    Ordered Locus Names:YMR172W
    ORF Names:YM8010.02
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR172w.
    SGDiS000004783. HOT1.

    Subcellular locationi

    Nucleus 2 Publications

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi30 – 301S → A: Impairs HOT1 phosphorylation; when associated with A-70; A-153; A-360 and A-410. 1 Publication
    Mutagenesisi70 – 701S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-153; A-360 and A-410. 1 Publication
    Mutagenesisi153 – 1531S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-360 and A-410. 1 Publication
    Mutagenesisi360 – 3601S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-153 and A-410. 1 Publication
    Mutagenesisi410 – 4101S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-153 and A-360. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 719719High-osmolarity-induced transcription protein 1PRO_0000203317Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei146 – 1461Phosphoserine1 Publication
    Modified residuei153 – 1531Phosphoserine1 Publication

    Post-translational modificationi

    Hyperphosphorylated during acute stress.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ03213.
    PaxDbiQ03213.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03213.

    Interactioni

    Subunit structurei

    Interacts with HOG1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HOG1P324854EBI-27376,EBI-8437
    RPD3P325613EBI-27376,EBI-15864

    Protein-protein interaction databases

    BioGridi35350. 31 interactions.
    DIPiDIP-2367N.
    IntActiQ03213. 8 interactions.
    MINTiMINT-616104.
    STRINGi4932.YMR172W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03213.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the HOT1 family.Curated

    Phylogenomic databases

    eggNOGiNOG43450.
    GeneTreeiENSGT00530000068684.
    OMAiTARICAP.
    OrthoDBiEOG7QK0NC.

    Family and domain databases

    InterProiIPR022210. TF_OSM.
    [Graphical view]
    PfamiPF12550. GCR1_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03213-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGMGIAILC IVRTKIYRIT ISFDYSTLMS PFFLFLMMPT TLKDGYRMNS    50
    QVNEDAIGIN LDLSLPTHIS PTTGSESASG SNASTLRNDG NALDGGLLRT 100
    SAAISAPTGT SQPTETIGEK LSNEERVNSN VSASNSTTAG TGRMLSQSLT 150
    NDSPSNEIST DQLKIFQRMD EMSARMIEME ESFNKLSNKI AEQNTMVLNL 200
    KQDNYKVMNK LNILLKLVAQ PSARPSTNNA QNKLAIELLN SISAVSSAYL 250
    QKMQNNGSGR QHTADLCTGD SNTHSGINQH RTTNGTIDVN TNTAQLNNQF 300
    SNALNTILPD QQHNRNNVSQ NINQSLPNRQ LGPVINTQAN QNQSQVLIHN 350
    TNTHQQVNRS PISFPNASTD KPFKLNPNGI KRRRRNTQSN NNASTNDHAS 400
    AAQKPISALS PLTNSHNSTT SMNYTNSSIH SGVTSASNSF HDLNSLNNFG 450
    TTTALSLPSL ALDNASFPPN QNVIPPIINN TQQPLSFSQL INQDSTTSEL 500
    LPSGKSGVNT NIVNRNRAST LPSYPKPMTV KSNVDDDGYQ EDDDDDGDDE 550
    GDGRDNEEDS TAEEDEVDDE IETDMKNASI NKRRRSLHHK KSNSLNGRRK 600
    LHGESATKPN INSDLHYRIL KAPTDVKTIW EEYDTGIRGK PSIKHLEAKY 650
    GNKWRLNKNK KTFSRRKRLY KFILNGMERG KTAQEMIETL ENKRLYKDDE 700
    DGEVKKRTIG WLQESLAGI 719
    Length:719
    Mass (Da):79,416
    Last modified:November 1, 1997 - v1
    Checksum:i4652BE93743D5A54
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49808 Genomic DNA. Translation: CAA89905.1.
    BK006946 Genomic DNA. Translation: DAA10068.1.
    PIRiS55119.
    RefSeqiNP_013895.1. NM_001182677.1.

    Genome annotation databases

    EnsemblFungiiYMR172W; YMR172W; YMR172W.
    GeneIDi855208.
    KEGGisce:YMR172W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49808 Genomic DNA. Translation: CAA89905.1 .
    BK006946 Genomic DNA. Translation: DAA10068.1 .
    PIRi S55119.
    RefSeqi NP_013895.1. NM_001182677.1.

    3D structure databases

    ProteinModelPortali Q03213.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35350. 31 interactions.
    DIPi DIP-2367N.
    IntActi Q03213. 8 interactions.
    MINTi MINT-616104.
    STRINGi 4932.YMR172W.

    Proteomic databases

    MaxQBi Q03213.
    PaxDbi Q03213.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR172W ; YMR172W ; YMR172W .
    GeneIDi 855208.
    KEGGi sce:YMR172W.

    Organism-specific databases

    CYGDi YMR172w.
    SGDi S000004783. HOT1.

    Phylogenomic databases

    eggNOGi NOG43450.
    GeneTreei ENSGT00530000068684.
    OMAi TARICAP.
    OrthoDBi EOG7QK0NC.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32860-MONOMER.

    Miscellaneous databases

    NextBioi 978708.

    Gene expression databases

    Genevestigatori Q03213.

    Family and domain databases

    InterProi IPR022210. TF_OSM.
    [Graphical view ]
    Pfami PF12550. GCR1_C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Osmotic stress-induced gene expression in Saccharomyces cerevisiae requires Msn1p and the novel nuclear factor Hot1p."
      Rep M., Reiser V., Gartner U., Thevelein J.M., Hohmann S., Ammerer G., Ruis H.
      Mol. Cell. Biol. 19:5474-5485(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH HOG1.
    4. "The transcriptional response of Saccharomyces cerevisiae to osmotic shock. Hot1p and Msn2p/Msn4p are required for the induction of subsets of high osmolarity glycerol pathway-dependent genes."
      Rep M., Krantz M., Thevelein J.M., Hohmann S.
      J. Biol. Chem. 275:8290-8300(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Stress-induced map kinase Hog1 is part of transcription activation complexes."
      Alepuz P.M., Jovanovic A., Reiser V., Ammerer G.
      Mol. Cell 7:767-777(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION, PROMOTER BINDING.
    6. "Osmostress-induced transcription by Hot1 depends on a Hog1-mediated recruitment of the RNA Pol II."
      Alepuz P.M., de Nadal E., Zapater M., Ammerer G., Posas F.
      EMBO J. 22:2433-2442(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HOG1, PHOSPHORYLATION, MUTAGENESIS OF SER-30; SER-70; SER-153; SER-360 AND SER-410.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-153, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHOT1_YEAST
    AccessioniPrimary (citable) accession number: Q03213
    Secondary accession number(s): D6VZZ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 149 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3