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Q03213

- HOT1_YEAST

UniProt

Q03213 - HOT1_YEAST

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Protein

High-osmolarity-induced transcription protein 1

Gene

HOT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for a complete transcriptional response to osmotic stress, through recruitment of HOG1 followed by pol II recruitment to the promoters of GPD1 and other HOG-dependent genes.4 Publications

GO - Molecular functioni

  1. RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly Source: SGD
  2. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: SGD

GO - Biological processi

  1. cellular hyperosmotic salinity response Source: SGD
  2. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  3. positive regulation of transcription from RNA polymerase II promoter in response to osmotic stress Source: SGD
  4. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32860-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
High-osmolarity-induced transcription protein 1
Gene namesi
Name:HOT1
Ordered Locus Names:YMR172W
ORF Names:YM8010.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR172w.
SGDiS000004783. HOT1.

Subcellular locationi

Nucleus 2 Publications

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi30 – 301S → A: Impairs HOT1 phosphorylation; when associated with A-70; A-153; A-360 and A-410. 1 Publication
Mutagenesisi70 – 701S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-153; A-360 and A-410. 1 Publication
Mutagenesisi153 – 1531S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-360 and A-410. 1 Publication
Mutagenesisi360 – 3601S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-153 and A-410. 1 Publication
Mutagenesisi410 – 4101S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-153 and A-360. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 719719High-osmolarity-induced transcription protein 1PRO_0000203317Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei146 – 1461Phosphoserine1 Publication
Modified residuei153 – 1531Phosphoserine1 Publication

Post-translational modificationi

Hyperphosphorylated during acute stress.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03213.
PaxDbiQ03213.

Expressioni

Gene expression databases

GenevestigatoriQ03213.

Interactioni

Subunit structurei

Interacts with HOG1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HOG1P324854EBI-27376,EBI-8437
RPD3P325613EBI-27376,EBI-15864

Protein-protein interaction databases

BioGridi35350. 31 interactions.
DIPiDIP-2367N.
IntActiQ03213. 8 interactions.
MINTiMINT-616104.
STRINGi4932.YMR172W.

Structurei

3D structure databases

ProteinModelPortaliQ03213.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HOT1 family.Curated

Phylogenomic databases

eggNOGiNOG43450.
GeneTreeiENSGT00530000068684.
InParanoidiQ03213.
OMAiTARICAP.
OrthoDBiEOG7QK0NC.

Family and domain databases

InterProiIPR022210. TF_OSM.
[Graphical view]
PfamiPF12550. GCR1_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03213-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGMGIAILC IVRTKIYRIT ISFDYSTLMS PFFLFLMMPT TLKDGYRMNS
60 70 80 90 100
QVNEDAIGIN LDLSLPTHIS PTTGSESASG SNASTLRNDG NALDGGLLRT
110 120 130 140 150
SAAISAPTGT SQPTETIGEK LSNEERVNSN VSASNSTTAG TGRMLSQSLT
160 170 180 190 200
NDSPSNEIST DQLKIFQRMD EMSARMIEME ESFNKLSNKI AEQNTMVLNL
210 220 230 240 250
KQDNYKVMNK LNILLKLVAQ PSARPSTNNA QNKLAIELLN SISAVSSAYL
260 270 280 290 300
QKMQNNGSGR QHTADLCTGD SNTHSGINQH RTTNGTIDVN TNTAQLNNQF
310 320 330 340 350
SNALNTILPD QQHNRNNVSQ NINQSLPNRQ LGPVINTQAN QNQSQVLIHN
360 370 380 390 400
TNTHQQVNRS PISFPNASTD KPFKLNPNGI KRRRRNTQSN NNASTNDHAS
410 420 430 440 450
AAQKPISALS PLTNSHNSTT SMNYTNSSIH SGVTSASNSF HDLNSLNNFG
460 470 480 490 500
TTTALSLPSL ALDNASFPPN QNVIPPIINN TQQPLSFSQL INQDSTTSEL
510 520 530 540 550
LPSGKSGVNT NIVNRNRAST LPSYPKPMTV KSNVDDDGYQ EDDDDDGDDE
560 570 580 590 600
GDGRDNEEDS TAEEDEVDDE IETDMKNASI NKRRRSLHHK KSNSLNGRRK
610 620 630 640 650
LHGESATKPN INSDLHYRIL KAPTDVKTIW EEYDTGIRGK PSIKHLEAKY
660 670 680 690 700
GNKWRLNKNK KTFSRRKRLY KFILNGMERG KTAQEMIETL ENKRLYKDDE
710
DGEVKKRTIG WLQESLAGI
Length:719
Mass (Da):79,416
Last modified:November 1, 1997 - v1
Checksum:i4652BE93743D5A54
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49808 Genomic DNA. Translation: CAA89905.1.
BK006946 Genomic DNA. Translation: DAA10068.1.
PIRiS55119.
RefSeqiNP_013895.1. NM_001182677.1.

Genome annotation databases

EnsemblFungiiYMR172W; YMR172W; YMR172W.
GeneIDi855208.
KEGGisce:YMR172W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49808 Genomic DNA. Translation: CAA89905.1 .
BK006946 Genomic DNA. Translation: DAA10068.1 .
PIRi S55119.
RefSeqi NP_013895.1. NM_001182677.1.

3D structure databases

ProteinModelPortali Q03213.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35350. 31 interactions.
DIPi DIP-2367N.
IntActi Q03213. 8 interactions.
MINTi MINT-616104.
STRINGi 4932.YMR172W.

Proteomic databases

MaxQBi Q03213.
PaxDbi Q03213.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR172W ; YMR172W ; YMR172W .
GeneIDi 855208.
KEGGi sce:YMR172W.

Organism-specific databases

CYGDi YMR172w.
SGDi S000004783. HOT1.

Phylogenomic databases

eggNOGi NOG43450.
GeneTreei ENSGT00530000068684.
InParanoidi Q03213.
OMAi TARICAP.
OrthoDBi EOG7QK0NC.

Enzyme and pathway databases

BioCyci YEAST:G3O-32860-MONOMER.

Miscellaneous databases

NextBioi 978708.

Gene expression databases

Genevestigatori Q03213.

Family and domain databases

InterProi IPR022210. TF_OSM.
[Graphical view ]
Pfami PF12550. GCR1_C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Osmotic stress-induced gene expression in Saccharomyces cerevisiae requires Msn1p and the novel nuclear factor Hot1p."
    Rep M., Reiser V., Gartner U., Thevelein J.M., Hohmann S., Ammerer G., Ruis H.
    Mol. Cell. Biol. 19:5474-5485(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH HOG1.
  4. "The transcriptional response of Saccharomyces cerevisiae to osmotic shock. Hot1p and Msn2p/Msn4p are required for the induction of subsets of high osmolarity glycerol pathway-dependent genes."
    Rep M., Krantz M., Thevelein J.M., Hohmann S.
    J. Biol. Chem. 275:8290-8300(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Stress-induced map kinase Hog1 is part of transcription activation complexes."
    Alepuz P.M., Jovanovic A., Reiser V., Ammerer G.
    Mol. Cell 7:767-777(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, PROMOTER BINDING.
  6. "Osmostress-induced transcription by Hot1 depends on a Hog1-mediated recruitment of the RNA Pol II."
    Alepuz P.M., de Nadal E., Zapater M., Ammerer G., Posas F.
    EMBO J. 22:2433-2442(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HOG1, PHOSPHORYLATION, MUTAGENESIS OF SER-30; SER-70; SER-153; SER-360 AND SER-410.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-153, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHOT1_YEAST
AccessioniPrimary (citable) accession number: Q03213
Secondary accession number(s): D6VZZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 149 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3