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Q03213 (HOT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
High-osmolarity-induced transcription protein 1
Gene names
Name:HOT1
Ordered Locus Names:YMR172W
ORF Names:YM8010.02
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length719 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for a complete transcriptional response to osmotic stress, through recruitment of HOG1 followed by pol II recruitment to the promoters of GPD1 and other HOG-dependent genes. Ref.3 Ref.4 Ref.5 Ref.6

Subunit structure

Interacts with HOG1. Ref.3 Ref.6

Subcellular location

Nucleus Ref.3 Ref.7.

Post-translational modification

Hyperphosphorylated during acute stress. Ref.5 Ref.6

Miscellaneous

Present with 149 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the HOT1 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HOG1P324854EBI-27376,EBI-8437
RPD3P325613EBI-27376,EBI-15864

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 719719High-osmolarity-induced transcription protein 1
PRO_0000203317

Amino acid modifications

Modified residue1461Phosphoserine Ref.9
Modified residue1531Phosphoserine Ref.10

Experimental info

Mutagenesis301S → A: Impairs HOT1 phosphorylation; when associated with A-70; A-153; A-360 and A-410. Ref.6
Mutagenesis701S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-153; A-360 and A-410. Ref.6
Mutagenesis1531S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-360 and A-410. Ref.6
Mutagenesis3601S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-153 and A-410. Ref.6
Mutagenesis4101S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-153 and A-360. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q03213 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 4652BE93743D5A54

FASTA71979,416
        10         20         30         40         50         60 
MSGMGIAILC IVRTKIYRIT ISFDYSTLMS PFFLFLMMPT TLKDGYRMNS QVNEDAIGIN 

        70         80         90        100        110        120 
LDLSLPTHIS PTTGSESASG SNASTLRNDG NALDGGLLRT SAAISAPTGT SQPTETIGEK 

       130        140        150        160        170        180 
LSNEERVNSN VSASNSTTAG TGRMLSQSLT NDSPSNEIST DQLKIFQRMD EMSARMIEME 

       190        200        210        220        230        240 
ESFNKLSNKI AEQNTMVLNL KQDNYKVMNK LNILLKLVAQ PSARPSTNNA QNKLAIELLN 

       250        260        270        280        290        300 
SISAVSSAYL QKMQNNGSGR QHTADLCTGD SNTHSGINQH RTTNGTIDVN TNTAQLNNQF 

       310        320        330        340        350        360 
SNALNTILPD QQHNRNNVSQ NINQSLPNRQ LGPVINTQAN QNQSQVLIHN TNTHQQVNRS 

       370        380        390        400        410        420 
PISFPNASTD KPFKLNPNGI KRRRRNTQSN NNASTNDHAS AAQKPISALS PLTNSHNSTT 

       430        440        450        460        470        480 
SMNYTNSSIH SGVTSASNSF HDLNSLNNFG TTTALSLPSL ALDNASFPPN QNVIPPIINN 

       490        500        510        520        530        540 
TQQPLSFSQL INQDSTTSEL LPSGKSGVNT NIVNRNRAST LPSYPKPMTV KSNVDDDGYQ 

       550        560        570        580        590        600 
EDDDDDGDDE GDGRDNEEDS TAEEDEVDDE IETDMKNASI NKRRRSLHHK KSNSLNGRRK 

       610        620        630        640        650        660 
LHGESATKPN INSDLHYRIL KAPTDVKTIW EEYDTGIRGK PSIKHLEAKY GNKWRLNKNK 

       670        680        690        700        710 
KTFSRRKRLY KFILNGMERG KTAQEMIETL ENKRLYKDDE DGEVKKRTIG WLQESLAGI 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Osmotic stress-induced gene expression in Saccharomyces cerevisiae requires Msn1p and the novel nuclear factor Hot1p."
Rep M., Reiser V., Gartner U., Thevelein J.M., Hohmann S., Ammerer G., Ruis H.
Mol. Cell. Biol. 19:5474-5485(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH HOG1.
[4]"The transcriptional response of Saccharomyces cerevisiae to osmotic shock. Hot1p and Msn2p/Msn4p are required for the induction of subsets of high osmolarity glycerol pathway-dependent genes."
Rep M., Krantz M., Thevelein J.M., Hohmann S.
J. Biol. Chem. 275:8290-8300(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Stress-induced map kinase Hog1 is part of transcription activation complexes."
Alepuz P.M., Jovanovic A., Reiser V., Ammerer G.
Mol. Cell 7:767-777(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION, PROMOTER BINDING.
[6]"Osmostress-induced transcription by Hot1 depends on a Hog1-mediated recruitment of the RNA Pol II."
Alepuz P.M., de Nadal E., Zapater M., Ammerer G., Posas F.
EMBO J. 22:2433-2442(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH HOG1, PHOSPHORYLATION, MUTAGENESIS OF SER-30; SER-70; SER-153; SER-360 AND SER-410.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-153, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49808 Genomic DNA. Translation: CAA89905.1.
BK006946 Genomic DNA. Translation: DAA10068.1.
PIRS55119.
RefSeqNP_013895.1. NM_001182677.1.

3D structure databases

ProteinModelPortalQ03213.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35350. 31 interactions.
DIPDIP-2367N.
IntActQ03213. 8 interactions.
MINTMINT-616104.
STRING4932.YMR172W.

Proteomic databases

PaxDbQ03213.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR172W; YMR172W; YMR172W.
GeneID855208.
KEGGsce:YMR172W.

Organism-specific databases

CYGDYMR172w.
SGDS000004783. HOT1.

Phylogenomic databases

eggNOGNOG43450.
GeneTreeENSGT00530000068684.
OMAKTIWEEY.
OrthoDBEOG7QK0NC.

Enzyme and pathway databases

BioCycYEAST:G3O-32860-MONOMER.

Gene expression databases

GenevestigatorQ03213.

Family and domain databases

InterProIPR022210. TF_OSM.
[Graphical view]
PfamPF12550. GCR1_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978708.

Entry information

Entry nameHOT1_YEAST
AccessionPrimary (citable) accession number: Q03213
Secondary accession number(s): D6VZZ4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families