Q03213 (HOT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
High-osmolarity-induced transcription protein 1

Gene names

Name:	HOT1
Ordered Locus Names:	YMR172W
ORF Names:	YM8010.02

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	719 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for a complete transcriptional response to osmotic stress, through recruitment of HOG1 followed by pol II recruitment to the promoters of GPD1 and other HOG-dependent genes. Ref.3 Ref.4 Ref.5 Ref.6
Subunit structure	Interacts with HOG1. Ref.3 Ref.6
Subcellular location	Nucleus Ref.3 Ref.7.
Post-translational modification	Hyperphosphorylated during acute stress. Ref.5 Ref.6
Miscellaneous	Present with 149 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the HOT1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular hyperosmotic salinity response Inferred from mutant phenotype Ref.3. Source: SGD positive regulation of transcription from RNA polymerase II promoter in response to osmotic stress Inferred from mutant phenotype Ref.3Ref.4. Source: SGD
Cellular_component	nucleus Inferred from direct assay Ref.3. Source: SGD
Molecular_function	RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HOG1	P32485	4	EBI-27376,EBI-8437
RPD3	P32561	3	EBI-27376,EBI-15864

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 719	719	High-osmolarity-induced transcription protein 1		PRO_0000203317
Amino acid modifications
	Modified residue	111	1	Phosphoserine Ref.9
	Modified residue	146	1	Phosphoserine Ref.9
	Modified residue	153	1	Phosphoserine Ref.9
	Modified residue	155	1	Phosphoserine Ref.9
	Modified residue	360	1	Phosphoserine Ref.9
Experimental info
	Mutagenesis	30	1	S → A: Impairs HOT1 phosphorylation; when associated with A-70; A-153; A-360 and A-410. Ref.6
	Mutagenesis	70	1	S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-153; A-360 and A-410. Ref.6
	Mutagenesis	153	1	S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-360 and A-410. Ref.6
	Mutagenesis	360	1	S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-153 and A-410. Ref.6
	Mutagenesis	410	1	S → A: Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-153 and A-360. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q03213 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 4652BE93743D5A54	FASTA	719	79,416
	10 20 30 40 50 60 MSGMGIAILC IVRTKIYRIT ISFDYSTLMS PFFLFLMMPT TLKDGYRMNS QVNEDAIGIN 70 80 90 100 110 120 LDLSLPTHIS PTTGSESASG SNASTLRNDG NALDGGLLRT SAAISAPTGT SQPTETIGEK 130 140 150 160 170 180 LSNEERVNSN VSASNSTTAG TGRMLSQSLT NDSPSNEIST DQLKIFQRMD EMSARMIEME 190 200 210 220 230 240 ESFNKLSNKI AEQNTMVLNL KQDNYKVMNK LNILLKLVAQ PSARPSTNNA QNKLAIELLN 250 260 270 280 290 300 SISAVSSAYL QKMQNNGSGR QHTADLCTGD SNTHSGINQH RTTNGTIDVN TNTAQLNNQF 310 320 330 340 350 360 SNALNTILPD QQHNRNNVSQ NINQSLPNRQ LGPVINTQAN QNQSQVLIHN TNTHQQVNRS 370 380 390 400 410 420 PISFPNASTD KPFKLNPNGI KRRRRNTQSN NNASTNDHAS AAQKPISALS PLTNSHNSTT 430 440 450 460 470 480 SMNYTNSSIH SGVTSASNSF HDLNSLNNFG TTTALSLPSL ALDNASFPPN QNVIPPIINN 490 500 510 520 530 540 TQQPLSFSQL INQDSTTSEL LPSGKSGVNT NIVNRNRAST LPSYPKPMTV KSNVDDDGYQ 550 560 570 580 590 600 EDDDDDGDDE GDGRDNEEDS TAEEDEVDDE IETDMKNASI NKRRRSLHHK KSNSLNGRRK 610 620 630 640 650 660 LHGESATKPN INSDLHYRIL KAPTDVKTIW EEYDTGIRGK PSIKHLEAKY GNKWRLNKNK 670 680 690 700 710 KTFSRRKRLY KFILNGMERG KTAQEMIETL ENKRLYKDDE DGEVKKRTIG WLQESLAGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Osmotic stress-induced gene expression in Saccharomyces cerevisiae requires Msn1p and the novel nuclear factor Hot1p." Rep M., Reiser V., Gartner U., Thevelein J.M., Hohmann S., Ammerer G., Ruis H. Mol. Cell. Biol. 19:5474-5485(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH HOG1.
[4]	"The transcriptional response of Saccharomyces cerevisiae to osmotic shock. Hot1p and Msn2p/Msn4p are required for the induction of subsets of high osmolarity glycerol pathway-dependent genes." Rep M., Krantz M., Thevelein J.M., Hohmann S. J. Biol. Chem. 275:8290-8300(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Stress-induced map kinase Hog1 is part of transcription activation complexes." Alepuz P.M., Jovanovic A., Reiser V., Ammerer G. Mol. Cell 7:767-777(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION, PROMOTER BINDING.
[6]	"Osmostress-induced transcription by Hot1 depends on a Hog1-mediated recruitment of the RNA Pol II." Alepuz P.M., de Nadal E., Zapater M., Ammerer G., Posas F. EMBO J. 22:2433-2442(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH HOG1, PHOSPHORYLATION, MUTAGENESIS OF SER-30; SER-70; SER-153; SER-360 AND SER-410.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-111; SER-146; SER-153; SER-155 AND SER-360, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49808 Genomic DNA. Translation: CAA89905.1. BK006946 Genomic DNA. Translation: DAA10068.1.
PIR	S55119.
RefSeq	NP_013895.1. NM_001182677.1.
3D structure databases
ProteinModelPortal	Q03213.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2367N.
IntAct	Q03213. 7 interactions.
MINT	MINT-616104.
STRING	4932.YMR172W.
Proteomic databases
PaxDb	Q03213.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR172W; YMR172W; YMR172W.
GeneID	855208.
KEGG	sce:YMR172W.
Organism-specific databases
CYGD	YMR172w.
SGD	S000004783. HOT1.
Phylogenomic databases
eggNOG	NOG43450.
GeneTree	ENSGT00530000068684.
OMA	KTIWEEY.
OrthoDB	EOG4JDMHN.
Gene expression databases
Genevestigator	Q03213.
GermOnline	YMR172W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022210. TF_OSM. [Graphical view]
Pfam	PF12550. GCR1_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978708.

Entry information

Entry name

HOT1_YEAST

Accession

Primary (citable) accession number: Q03213
Secondary accession number(s): D6VZZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program