Q03210 (NGL3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable RNA exonuclease NGL3
EC=3.1.-.-

Gene names

Name:	NGL3
Ordered Locus Names:	YML118W
ORF Names:	YM7056.08, YM8339.01

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Miscellaneous	Present with 414 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the CCR4/nocturin family.

Ontologies

Keywords
Molecular function	Exonuclease Hydrolase Nuclease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	polyadenylation-dependent RNA catabolic process Inferred from direct assay. Source: SGD
Molecular function	poly(A)-specific ribonuclease activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Probable RNA exonuclease NGL3		PRO_0000218580
Amino acid modifications
	Modified residue	62	1	Phosphoserine Ref.4 Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q03210 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 2B6D6389A93B7D72	FASTA	505	57,742
	10 20 30 40 50 60 MDSQVEGKIS PSQKESSSTS GLVSPSEDGP AHQKIHRDQL SVDQIKKIRE ERAQKRQVRR 70 80 90 100 110 120 NSLISQGKDP DFPTPDLQFI ERPFLPINHD NSKGLTPATI QVTQDSLDVK IMTYNTLAQT 130 140 150 160 170 180 LIRRDFFPES GPALKWHKRS KVLVHELKKY RPDVVSLQEV DYNELNFWQE NFHKLGFDVI 190 200 210 220 230 240 FKRHEGKTHG LLVAWNNKKF QLDNDWMLDY DNILAGNVIS ARTRTKNIAL IISLYFKGIT 250 260 270 280 290 300 DSSSRGIIVA NTHLFWHPFG VFERLRQSYL VLQKIQEIKA CSKYNGWHSL LMGDFNTEPE 310 320 330 340 350 360 EPPYLAITKR PLILKGPIRA MVECSLAYRY SKKRNGEESD QDDEECDEKS RGEGHSDQPQ 370 380 390 400 410 420 NPKPESFTAT KEEKALVNQL VALHNSLHVK GVSLYGIGYG KVHPENANGS HGEPGLSNWA 430 440 450 460 470 480 NTWCGLLDYI FYIEGDHNQD TRQKEPLNAF EGNNNVKIIG YLRMPCAQEM PKHSQPFEGE 490 500 YASDHISLMC QIRLFFGGEK VHSLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62, MASS SPECTROMETRY.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49218 Genomic DNA. Translation: CAA89162.1. Z49210 Genomic DNA. Translation: CAA89100.1. BK006946 Genomic DNA. Translation: DAA09780.1.
PIR	S54066.
RefSeq	NP_013588.1. NM_001182481.1.
3D structure databases
ProteinModelPortal	Q03210.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q03210. 1 interaction.
MINT	MINT-4084056.
STRING	Q03210.
Proteomic databases
PRIDE	Q03210.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML118W; YML118W; YML118W.
GeneID	854921.
KEGG	sce:YML118W.
NMPDR	fig|4932.3.peg.4624.
Organism-specific databases
CYGD	YML118w.
SGD	S000004587. NGL3.
Phylogenomic databases
eggNOG	fuNOG04481.
GeneTree	EFGT00050000002545.
HOGENOM	HBG397568.
OMA	FETANNI.
OrthoDB	EOG4K3Q4X.
Gene expression databases
ArrayExpress	Q03210.
Genevestigator	Q03210.
GermOnline	YML118W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005135. Endo/exonuclease/phosphatase. [Graphical view]
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
SUPFAM	SSF56219. Exo_endo_phos. 1 hit.
ProtoNet	Search...
Other
NextBio	977935.

Entry information

Entry name

NGL3_YEAST

Accession

Primary (citable) accession number: Q03210
Secondary accession number(s): D6W0G6, Q03733

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program