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Q031X8 (DDL_LACLS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:LACR_0387
OrganismLactococcus lactis subsp. cremoris (strain SK11) [Complete proteome] [HAMAP]
Taxonomic identifier272622 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence caution

The sequence ABJ71994.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341124

Regions

Domain132 – 335204ATP-grasp
Nucleotide binding162 – 21756ATP By similarity

Sites

Metal binding2891Magnesium or manganese 1 By similarity
Metal binding3021Magnesium or manganese 1 By similarity
Metal binding3021Magnesium or manganese 2 By similarity
Metal binding3041Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q031X8 [UniParc].

Last modified June 10, 2008. Version 2.
Checksum: 5E121E70EB21AB80

FASTA34938,609
        10         20         30         40         50         60 
MSKETLILLY GGRSAEREVS VLSAESVMRA VNYSRFIVKT YFITKGGEFI KTQEFTDTPA 

        70         80         90        100        110        120 
EEEKLLTNDL AESYPKISPA AIYEKDAVVF PVLHGPMGED GSIQGFLEIL RLAYVGPNIL 

       130        140        150        160        170        180 
SASSTMDKLL AKHVFEAVGV PQVPYVAAFA DENQAEIAQE VVEKLEFPVF VKPANMGSSV 

       190        200        210        220        230        240 
GISKVDDLAD LQPALSEAYK YDNRVVIEQG VDAREIECAV LGNNSDVSAT LPGEVVKDVG 

       250        260        270        280        290        300 
FYDYNSKYID NKIQMDIPAK VSADLAQKIQ EYAKKAYKAV NGAGLSRCDF FVTKDGNVYL 

       310        320        330        340 
NEVNAIPGFT QWSMYPLLWE NMGLSYSDLI EKLVDLAKET FETRENHLL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000425 Genomic DNA. Translation: ABJ71994.1. Different initiation.
RefSeqYP_808416.1. NC_008527.1.

3D structure databases

ProteinModelPortalQ031X8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272622.LACR_0387.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ71994; ABJ71994; LACR_0387.
GeneID4433819.
KEGGllc:LACR_0387.
PATRIC22287539. VBILacLac38071_0547.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycLLAC272622:GJUG-387-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_LACLS
AccessionPrimary (citable) accession number: Q031X8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: May 14, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways