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Q03195

- RLI1_YEAST

UniProt

Q03195 - RLI1_YEAST

Protein

Translation initiation factor RLI1

Gene

RLI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Component of the multifactor complex (MFC) involved in translation initiation. Required for the binding of MFC to the 40S ribosome. Required for the processing and nuclear export of the 60S and 40S ribosomal subunits.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi110 – 1178ATPPROSITE-ProRule annotation
    Nucleotide bindingi385 – 3928ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATPase activity Source: SGD
    2. ATP binding Source: UniProtKB-KW
    3. iron ion binding Source: SGD
    4. iron-sulfur cluster binding Source: InterPro
    5. protein binding Source: UniProtKB
    6. translation initiation factor activity Source: UniProtKB-KW

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. cellular response to oxidative stress Source: SGD
    3. positive regulation of translation Source: SGD
    4. ribosomal large subunit biogenesis Source: SGD
    5. ribosomal subunit export from nucleus Source: SGD
    6. ribosome disassembly Source: SGD
    7. rRNA processing Source: UniProtKB-KW
    8. translational initiation Source: SGD
    9. translational termination Source: SGD

    Keywords - Molecular functioni

    Initiation factor

    Keywords - Biological processi

    Protein biosynthesis, Ribosome biogenesis, rRNA processing, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29696-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Translation initiation factor RLI1
    Alternative name(s):
    ATP-binding cassette sub-family E member RLI1
    RNase L inhibitor
    Gene namesi
    Name:RLI1
    Ordered Locus Names:YDR091C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR091c.
    SGDiS000002498. RLI1.

    Subcellular locationi

    Cytoplasm. Nucleus
    Note: Shuttles between the nucleus and the cytoplasm.

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. cytosolic ribosome Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi224 – 2241G → D: Lethal; when associated with D-225. 1 Publication
    Mutagenesisi225 – 2251G → D: Lethal; when associated with D-224. 1 Publication
    Mutagenesisi470 – 4701G → D: Lethal; when associated with D-471. 1 Publication
    Mutagenesisi471 – 4711G → D: Lethal; when associated with D-470. 1 Publication
    Mutagenesisi493 – 4931E → Q: Lethal. Inhibits translation in vitro. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 608608Translation initiation factor RLI1PRO_0000268703Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei349 – 3491Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ03195.
    PaxDbiQ03195.
    PeptideAtlasiQ03195.

    Expressioni

    Gene expression databases

    GenevestigatoriQ03195.

    Interactioni

    Subunit structurei

    Component of the multifactor complex (MFC) composed of at least RLI1, the eIF2 subunit SUI2, TIF5/eIF5, and the eIF3 subunits PRT1, HCR1, NIP1, RPG1, TIF34 and TIF35. The complex associates with preinitiation complexes.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HCR1Q057754EBI-35146,EBI-8944
    SUP35Q6Q7I02EBI-35146,EBI-7724365From a different organism.
    SUP45P123855EBI-35146,EBI-6533

    Protein-protein interaction databases

    BioGridi32148. 116 interactions.
    DIPiDIP-4492N.
    IntActiQ03195. 42 interactions.
    MINTiMINT-472764.
    STRINGi4932.YDR091C.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3J16electron microscopy-B1-608[»]
    ProteinModelPortaliQ03195.
    SMRiQ03195. Positions 4-603.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 39334Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini46 – 75304Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini70 – 320251ABC transporter 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini345 – 568224ABC transporter 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation
    Contains 2 ABC transporter domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1245.
    GeneTreeiENSGT00390000015089.
    HOGENOMiHOG000222803.
    KOiK06174.
    OMAiRYILHAK.
    OrthoDBiEOG7G1VFV.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR003593. AAA+_ATPase.
    IPR013283. ABC_E.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR027417. P-loop_NTPase.
    IPR007209. RNaseL-inhib_metal-bd_dom.
    [Graphical view]
    PfamiPF00005. ABC_tran. 2 hits.
    PF00037. Fer4. 1 hit.
    PF04068. RLI. 1 hit.
    [Graphical view]
    PRINTSiPR01868. ABCEFAMILY.
    SMARTiSM00382. AAA. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 3 hits.
    PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
    PS51379. 4FE4S_FER_2. 2 hits.
    PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03195-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDKNSRIAI VSADKCKPKK CRQECKRSCP VVKTGKLCIE VTPTSKIAFI    50
    SEILCIGCGI CVKKCPFDAI QIINLPTNLE AHVTHRYSAN SFKLHRLPTP 100
    RPGQVLGLVG TNGIGKSTAL KILAGKQKPN LGRFDDPPEW QEIIKYFRGS 150
    ELQNYFTKML EDDIKAIIKP QYVDNIPRAI KGPVQKVGEL LKLRMEKSPE 200
    DVKRYIKILQ LENVLKRDIE KLSGGELQRF AIGMSCVQEA DVYMFDEPSS 250
    YLDVKQRLNA AQIIRSLLAP TKYVICVEHD LSVLDYLSDF VCIIYGVPSV 300
    YGVVTLPASV REGINIFLDG HIPAENLRFR TEALQFRIAD ATEDLQNDSA 350
    SRAFSYPSLK KTQGDFVLNV EEGEFSDSEI LVMMGENGTG KTTLIKLLAG 400
    ALKPDEGQDI PKLNVSMKPQ KIAPKFPGTV RQLFFKKIRG QFLNPQFQTD 450
    VVKPLRIDDI IDQEVQHLSG GELQRVAIVL ALGIPADIYL IDEPSAYLDS 500
    EQRIICSKVI RRFILHNKKT AFIVEHDFIM ATYLADKVIV FEGIPSKNAH 550
    ARAPESLLTG CNRFLKNLNV TFRRDPNSFR PRINKLDSQM DKEQKSSGNY 600
    FFLDNTGI 608
    Length:608
    Mass (Da):68,340
    Last modified:November 1, 1996 - v1
    Checksum:i1E76EC3D0457963D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z50111 Genomic DNA. Translation: CAA90450.1.
    AY723776 Genomic DNA. Translation: AAU09693.1.
    BK006938 Genomic DNA. Translation: DAA11938.1.
    PIRiS58091.
    RefSeqiNP_010376.3. NM_001180399.3.

    Genome annotation databases

    EnsemblFungiiYDR091C; YDR091C; YDR091C.
    GeneIDi851665.
    KEGGisce:YDR091C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z50111 Genomic DNA. Translation: CAA90450.1 .
    AY723776 Genomic DNA. Translation: AAU09693.1 .
    BK006938 Genomic DNA. Translation: DAA11938.1 .
    PIRi S58091.
    RefSeqi NP_010376.3. NM_001180399.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3J16 electron microscopy - B 1-608 [» ]
    ProteinModelPortali Q03195.
    SMRi Q03195. Positions 4-603.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32148. 116 interactions.
    DIPi DIP-4492N.
    IntActi Q03195. 42 interactions.
    MINTi MINT-472764.
    STRINGi 4932.YDR091C.

    Proteomic databases

    MaxQBi Q03195.
    PaxDbi Q03195.
    PeptideAtlasi Q03195.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR091C ; YDR091C ; YDR091C .
    GeneIDi 851665.
    KEGGi sce:YDR091C.

    Organism-specific databases

    CYGDi YDR091c.
    SGDi S000002498. RLI1.

    Phylogenomic databases

    eggNOGi COG1245.
    GeneTreei ENSGT00390000015089.
    HOGENOMi HOG000222803.
    KOi K06174.
    OMAi RYILHAK.
    OrthoDBi EOG7G1VFV.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29696-MONOMER.

    Miscellaneous databases

    NextBioi 969280.
    PROi Q03195.

    Gene expression databases

    Genevestigatori Q03195.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR003593. AAA+_ATPase.
    IPR013283. ABC_E.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR027417. P-loop_NTPase.
    IPR007209. RNaseL-inhib_metal-bd_dom.
    [Graphical view ]
    Pfami PF00005. ABC_tran. 2 hits.
    PF00037. Fer4. 1 hit.
    PF04068. RLI. 1 hit.
    [Graphical view ]
    PRINTSi PR01868. ABCEFAMILY.
    SMARTi SM00382. AAA. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 3 hits.
    PROSITEi PS00198. 4FE4S_FER_1. 1 hit.
    PS51379. 4FE4S_FER_2. 2 hits.
    PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "The essential ATP-binding cassette protein RLI1 functions in translation by promoting preinitiation complex assembly."
      Dong J., Lai R., Nielsen K., Fekete C.A., Qiu H., Hinnebusch A.G.
      J. Biol. Chem. 279:42157-42168(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE MFC COMPLEX, MUTAGENESIS OF GLY-224; GLY-225; GLY-470; GLY-471 AND GLU-493, SUBCELLULAR LOCATION.
    7. "Functional link between ribosome formation and biogenesis of iron-sulfur proteins."
      Yarunin A., Panse V.G., Petfalski E., Dez C., Tollervey D., Hurt E.C.
      EMBO J. 24:580-588(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, ASSOCIATION WITH PRE-RIBOSOMAL PARTICLES, IDENTIFICATION IN THE MFC COMPLEX.
    8. "Biogenesis of cytosolic ribosomes requires the essential iron-sulphur protein Rli1p and mitochondria."
      Kispal G., Sipos K., Lange H., Fekete Z., Bedekovics T., Janaky T., Bassler J., Aguilar Netz D.J., Balk J., Rotte C., Lill R.
      EMBO J. 24:589-598(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, ASSOCIATION WITH PRE-RIBOSOMAL PARTICLES, IDENTIFICATION IN THE MFC COMPLEX.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-349, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRLI1_YEAST
    AccessioniPrimary (citable) accession number: Q03195
    Secondary accession number(s): D6VS78
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 12, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6280 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3