Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Translation initiation factor RLI1

Gene

RLI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the multifactor complex (MFC) involved in translation initiation. Required for the binding of MFC to the 40S ribosome. Required for the processing and nuclear export of the 60S and 40S ribosomal subunits.3 Publications

Miscellaneous

Present with 6280 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi110 – 117ATPPROSITE-ProRule annotation8
Nucleotide bindingi385 – 392ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: GO_Central
  • iron ion binding Source: SGD
  • ribosomal small subunit binding Source: GO_Central
  • translation initiation factor activity Source: UniProtKB-KW

GO - Biological processi

  • positive regulation of translation Source: SGD
  • ribosomal large subunit biogenesis Source: SGD
  • ribosomal subunit export from nucleus Source: SGD
  • ribosome disassembly Source: SGD
  • rRNA processing Source: UniProtKB-KW
  • translational initiation Source: SGD
  • translational termination Source: SGD

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis, Ribosome biogenesis, rRNA processing, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29696-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor RLI1
Alternative name(s):
ATP-binding cassette sub-family E member RLI1
RNase L inhibitor
Gene namesi
Name:RLI1
Ordered Locus Names:YDR091C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR091C
SGDiS000002498 RLI1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi224G → D: Lethal; when associated with D-225. 1 Publication1
Mutagenesisi225G → D: Lethal; when associated with D-224. 1 Publication1
Mutagenesisi470G → D: Lethal; when associated with D-471. 1 Publication1
Mutagenesisi471G → D: Lethal; when associated with D-470. 1 Publication1
Mutagenesisi493E → Q: Lethal. Inhibits translation in vitro. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002687031 – 608Translation initiation factor RLI1Add BLAST608

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei349PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03195
PaxDbiQ03195
PRIDEiQ03195
TopDownProteomicsiQ03195

PTM databases

iPTMnetiQ03195

Interactioni

Subunit structurei

Component of the multifactor complex (MFC) composed of at least RLI1, the eIF2 subunit SUI2, TIF5/eIF5, and the eIF3 subunits PRT1, HCR1, NIP1, RPG1, TIF34 and TIF35. The complex associates with preinitiation complexes.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32148, 175 interactors
DIPiDIP-4492N
IntActiQ03195, 50 interactors
MINTiQ03195
STRINGi4932.YDR091C

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J16electron microscopy-B1-608[»]
4CRMelectron microscopy8.75P1-608[»]
5LL6electron microscopy3.90h1-608[»]
ProteinModelPortaliQ03195
SMRiQ03195
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 394Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST33
Domaini46 – 754Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST30
Domaini70 – 320ABC transporter 1PROSITE-ProRule annotationAdd BLAST251
Domaini345 – 568ABC transporter 2PROSITE-ProRule annotationAdd BLAST224

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000015089
HOGENOMiHOG000222803
InParanoidiQ03195
KOiK06174
OMAiTGCNTFL
OrthoDBiEOG092C11NN

Family and domain databases

CDDicd03236 ABC_RNaseL_inhibitor_domain1, 1 hit
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR003593 AAA+_ATPase
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR027417 P-loop_NTPase
IPR013283 RLI1
IPR034348 RLI_dom_1
IPR007209 RNaseL-inhib_metal-bd_dom
PANTHERiPTHR19248 PTHR19248, 1 hit
PfamiView protein in Pfam
PF00005 ABC_tran, 2 hits
PF00037 Fer4, 1 hit
PF04068 RLI, 1 hit
PRINTSiPR01868 ABCEFAMILY
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SUPFAMiSSF52540 SSF52540, 3 hits
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 1 hit
PS51379 4FE4S_FER_2, 2 hits
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 2 hits

Sequencei

Sequence statusi: Complete.

Q03195-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDKNSRIAI VSADKCKPKK CRQECKRSCP VVKTGKLCIE VTPTSKIAFI
60 70 80 90 100
SEILCIGCGI CVKKCPFDAI QIINLPTNLE AHVTHRYSAN SFKLHRLPTP
110 120 130 140 150
RPGQVLGLVG TNGIGKSTAL KILAGKQKPN LGRFDDPPEW QEIIKYFRGS
160 170 180 190 200
ELQNYFTKML EDDIKAIIKP QYVDNIPRAI KGPVQKVGEL LKLRMEKSPE
210 220 230 240 250
DVKRYIKILQ LENVLKRDIE KLSGGELQRF AIGMSCVQEA DVYMFDEPSS
260 270 280 290 300
YLDVKQRLNA AQIIRSLLAP TKYVICVEHD LSVLDYLSDF VCIIYGVPSV
310 320 330 340 350
YGVVTLPASV REGINIFLDG HIPAENLRFR TEALQFRIAD ATEDLQNDSA
360 370 380 390 400
SRAFSYPSLK KTQGDFVLNV EEGEFSDSEI LVMMGENGTG KTTLIKLLAG
410 420 430 440 450
ALKPDEGQDI PKLNVSMKPQ KIAPKFPGTV RQLFFKKIRG QFLNPQFQTD
460 470 480 490 500
VVKPLRIDDI IDQEVQHLSG GELQRVAIVL ALGIPADIYL IDEPSAYLDS
510 520 530 540 550
EQRIICSKVI RRFILHNKKT AFIVEHDFIM ATYLADKVIV FEGIPSKNAH
560 570 580 590 600
ARAPESLLTG CNRFLKNLNV TFRRDPNSFR PRINKLDSQM DKEQKSSGNY

FFLDNTGI
Length:608
Mass (Da):68,340
Last modified:November 1, 1996 - v1
Checksum:i1E76EC3D0457963D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50111 Genomic DNA Translation: CAA90450.1
AY723776 Genomic DNA Translation: AAU09693.1
BK006938 Genomic DNA Translation: DAA11938.1
PIRiS58091
RefSeqiNP_010376.3, NM_001180399.3

Genome annotation databases

EnsemblFungiiYDR091C; YDR091C; YDR091C
GeneIDi851665
KEGGisce:YDR091C

Similar proteinsi

Entry informationi

Entry nameiRLI1_YEAST
AccessioniPrimary (citable) accession number: Q03195
Secondary accession number(s): D6VS78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health