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Q03195

- RLI1_YEAST

UniProt

Q03195 - RLI1_YEAST

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Protein
Translation initiation factor RLI1
Gene
RLI1, YDR091C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the multifactor complex (MFC) involved in translation initiation. Required for the binding of MFC to the 40S ribosome. Required for the processing and nuclear export of the 60S and 40S ribosomal subunits.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi110 – 1178ATP Reviewed prediction
Nucleotide bindingi385 – 3928ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATPase activity Source: SGD
  3. iron ion binding Source: SGD
  4. iron-sulfur cluster binding Source: InterPro
  5. protein binding Source: UniProtKB
  6. translation initiation factor activity Source: UniProtKB-KW

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. cellular response to oxidative stress Source: SGD
  3. positive regulation of translation Source: SGD
  4. rRNA processing Source: UniProtKB-KW
  5. ribosomal large subunit biogenesis Source: SGD
  6. ribosomal subunit export from nucleus Source: SGD
  7. ribosome disassembly Source: SGD
  8. translational initiation Source: SGD
  9. translational termination Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Ribosome biogenesis, rRNA processing, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29696-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor RLI1
Alternative name(s):
ATP-binding cassette sub-family E member RLI1
RNase L inhibitor
Gene namesi
Name:RLI1
Ordered Locus Names:YDR091C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR091c.
SGDiS000002498. RLI1.

Subcellular locationi

Cytoplasm. Nucleus
Note: Shuttles between the nucleus and the cytoplasm.4 Publications

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. cytosolic ribosome Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi224 – 2241G → D: Lethal; when associated with D-225. 1 Publication
Mutagenesisi225 – 2251G → D: Lethal; when associated with D-224. 1 Publication
Mutagenesisi470 – 4701G → D: Lethal; when associated with D-471. 1 Publication
Mutagenesisi471 – 4711G → D: Lethal; when associated with D-470. 1 Publication
Mutagenesisi493 – 4931E → Q: Lethal. Inhibits translation in vitro. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 608608Translation initiation factor RLI1
PRO_0000268703Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei349 – 3491Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03195.
PaxDbiQ03195.
PeptideAtlasiQ03195.

Expressioni

Gene expression databases

GenevestigatoriQ03195.

Interactioni

Subunit structurei

Component of the multifactor complex (MFC) composed of at least RLI1, the eIF2 subunit SUI2, TIF5/eIF5, and the eIF3 subunits PRT1, HCR1, NIP1, RPG1, TIF34 and TIF35. The complex associates with preinitiation complexes.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HCR1Q057754EBI-35146,EBI-8944
SUP35Q6Q7I02EBI-35146,EBI-7724365From a different organism.
SUP45P123855EBI-35146,EBI-6533

Protein-protein interaction databases

BioGridi32148. 116 interactions.
DIPiDIP-4492N.
IntActiQ03195. 42 interactions.
MINTiMINT-472764.
STRINGi4932.YDR091C.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J16electron microscopy-B1-608[»]
ProteinModelPortaliQ03195.
SMRiQ03195. Positions 4-603.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 39334Fe-4S ferredoxin-type 1
Add
BLAST
Domaini46 – 75304Fe-4S ferredoxin-type 2
Add
BLAST
Domaini70 – 320251ABC transporter 1
Add
BLAST
Domaini345 – 568224ABC transporter 2
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1245.
GeneTreeiENSGT00390000015089.
HOGENOMiHOG000222803.
KOiK06174.
OMAiRYILHAK.
OrthoDBiEOG7G1VFV.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR003593. AAA+_ATPase.
IPR013283. ABC_E.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR007209. RNaseL-inhib_metal-bd_dom.
[Graphical view]
PfamiPF00005. ABC_tran. 2 hits.
PF00037. Fer4. 1 hit.
PF04068. RLI. 1 hit.
[Graphical view]
PRINTSiPR01868. ABCEFAMILY.
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03195-1 [UniParc]FASTAAdd to Basket

« Hide

MSDKNSRIAI VSADKCKPKK CRQECKRSCP VVKTGKLCIE VTPTSKIAFI    50
SEILCIGCGI CVKKCPFDAI QIINLPTNLE AHVTHRYSAN SFKLHRLPTP 100
RPGQVLGLVG TNGIGKSTAL KILAGKQKPN LGRFDDPPEW QEIIKYFRGS 150
ELQNYFTKML EDDIKAIIKP QYVDNIPRAI KGPVQKVGEL LKLRMEKSPE 200
DVKRYIKILQ LENVLKRDIE KLSGGELQRF AIGMSCVQEA DVYMFDEPSS 250
YLDVKQRLNA AQIIRSLLAP TKYVICVEHD LSVLDYLSDF VCIIYGVPSV 300
YGVVTLPASV REGINIFLDG HIPAENLRFR TEALQFRIAD ATEDLQNDSA 350
SRAFSYPSLK KTQGDFVLNV EEGEFSDSEI LVMMGENGTG KTTLIKLLAG 400
ALKPDEGQDI PKLNVSMKPQ KIAPKFPGTV RQLFFKKIRG QFLNPQFQTD 450
VVKPLRIDDI IDQEVQHLSG GELQRVAIVL ALGIPADIYL IDEPSAYLDS 500
EQRIICSKVI RRFILHNKKT AFIVEHDFIM ATYLADKVIV FEGIPSKNAH 550
ARAPESLLTG CNRFLKNLNV TFRRDPNSFR PRINKLDSQM DKEQKSSGNY 600
FFLDNTGI 608
Length:608
Mass (Da):68,340
Last modified:November 1, 1996 - v1
Checksum:i1E76EC3D0457963D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z50111 Genomic DNA. Translation: CAA90450.1.
AY723776 Genomic DNA. Translation: AAU09693.1.
BK006938 Genomic DNA. Translation: DAA11938.1.
PIRiS58091.
RefSeqiNP_010376.3. NM_001180399.3.

Genome annotation databases

EnsemblFungiiYDR091C; YDR091C; YDR091C.
GeneIDi851665.
KEGGisce:YDR091C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z50111 Genomic DNA. Translation: CAA90450.1 .
AY723776 Genomic DNA. Translation: AAU09693.1 .
BK006938 Genomic DNA. Translation: DAA11938.1 .
PIRi S58091.
RefSeqi NP_010376.3. NM_001180399.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3J16 electron microscopy - B 1-608 [» ]
ProteinModelPortali Q03195.
SMRi Q03195. Positions 4-603.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32148. 116 interactions.
DIPi DIP-4492N.
IntActi Q03195. 42 interactions.
MINTi MINT-472764.
STRINGi 4932.YDR091C.

Proteomic databases

MaxQBi Q03195.
PaxDbi Q03195.
PeptideAtlasi Q03195.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR091C ; YDR091C ; YDR091C .
GeneIDi 851665.
KEGGi sce:YDR091C.

Organism-specific databases

CYGDi YDR091c.
SGDi S000002498. RLI1.

Phylogenomic databases

eggNOGi COG1245.
GeneTreei ENSGT00390000015089.
HOGENOMi HOG000222803.
KOi K06174.
OMAi RYILHAK.
OrthoDBi EOG7G1VFV.

Enzyme and pathway databases

BioCyci YEAST:G3O-29696-MONOMER.

Miscellaneous databases

NextBioi 969280.
PROi Q03195.

Gene expression databases

Genevestigatori Q03195.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR003593. AAA+_ATPase.
IPR013283. ABC_E.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR007209. RNaseL-inhib_metal-bd_dom.
[Graphical view ]
Pfami PF00005. ABC_tran. 2 hits.
PF00037. Fer4. 1 hit.
PF04068. RLI. 1 hit.
[Graphical view ]
PRINTSi PR01868. ABCEFAMILY.
SMARTi SM00382. AAA. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 3 hits.
PROSITEi PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "The essential ATP-binding cassette protein RLI1 functions in translation by promoting preinitiation complex assembly."
    Dong J., Lai R., Nielsen K., Fekete C.A., Qiu H., Hinnebusch A.G.
    J. Biol. Chem. 279:42157-42168(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE MFC COMPLEX, MUTAGENESIS OF GLY-224; GLY-225; GLY-470; GLY-471 AND GLU-493, SUBCELLULAR LOCATION.
  7. "Functional link between ribosome formation and biogenesis of iron-sulfur proteins."
    Yarunin A., Panse V.G., Petfalski E., Dez C., Tollervey D., Hurt E.C.
    EMBO J. 24:580-588(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, ASSOCIATION WITH PRE-RIBOSOMAL PARTICLES, IDENTIFICATION IN THE MFC COMPLEX.
  8. "Biogenesis of cytosolic ribosomes requires the essential iron-sulphur protein Rli1p and mitochondria."
    Kispal G., Sipos K., Lange H., Fekete Z., Bedekovics T., Janaky T., Bassler J., Aguilar Netz D.J., Balk J., Rotte C., Lill R.
    EMBO J. 24:589-598(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, ASSOCIATION WITH PRE-RIBOSOMAL PARTICLES, IDENTIFICATION IN THE MFC COMPLEX.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-349, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRLI1_YEAST
AccessioniPrimary (citable) accession number: Q03195
Secondary accession number(s): D6VS78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6280 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

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