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Q03194 (PMA4_NICPL) Reviewed, UniProtKB/Swiss-Prot

Last modified October 19, 2011. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Plasma membrane ATPase 4
EC=3.6.3.6
Alternative name(s):
Proton pump 4
Gene names
Name:PMA4
OrganismNicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco)
Taxonomic identifier4092 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length952 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activity

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Expressed at high levels in root, stem, leaf and flower.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 952952Plasma membrane ATPase 4
PRO_0000046293

Regions

Topological domain1 – 6464Cytoplasmic Potential
Transmembrane65 – 8420Helical; Name=1; Potential
Topological domain85 – 9612Extracellular Potential
Transmembrane97 – 11721Helical; Name=2; Potential
Topological domain118 – 246129Cytoplasmic Potential
Transmembrane247 – 26721Helical; Name=3; Potential
Topological domain268 – 27710Extracellular Potential
Transmembrane278 – 29922Helical; Name=4; Potential
Topological domain300 – 646347Cytoplasmic Potential
Transmembrane647 – 66822Helical; Name=5; Potential
Topological domain669 – 6735Extracellular Potential
Transmembrane674 – 69623Helical; Name=6; Potential
Topological domain697 – 71216Cytoplasmic Potential
Transmembrane713 – 73321Helical; Name=7; Potential
Topological domain734 – 75421Extracellular Potential
Transmembrane755 – 77521Helical; Name=8; Potential
Topological domain776 – 78712Cytoplasmic Potential
Transmembrane788 – 80821Helical; Name=9; Potential
Topological domain809 – 8179Extracellular Potential
Transmembrane818 – 83821Helical; Name=10; Potential
Topological domain839 – 952114Cytoplasmic Potential

Sites

Active site33214-aspartylphosphate intermediate By similarity
Metal binding5911Magnesium By similarity
Metal binding5951Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q03194 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: FD66F994796B3C6F

FASTA952105,189
        10         20         30         40         50         60 
MAKAISLEEI KNETVDLEKI PIEEVFEQLK CTREGLSADE GASRLQIFGP NKLEEKNESK 

        70         80         90        100        110        120 
ILKFLGFMWN PLSWVMEAAA VMAIALANGD GKPPDWQDFI GIICLLVINS TISFIEENNA 

       130        140        150        160        170        180 
GNAAAALMAG LAPKTKVLRD GRWSEQEAAI LVPGDIISVK LGDIIPADAR LLEGDPLKID 

       190        200        210        220        230        240 
QSALTGESLP VTKNPGDEVF SGSTCKQGEL EAVVIATGVH TFFGKAAHLV DSTNNVGHFQ 

       250        260        270        280        290        300 
KVLTAIGNFC ICSIAIGMLV EIIVMYPIQH RKYRDGIDNL LVLLIGGIPI AMPTVLSVTM 

       310        320        330        340        350        360 
AIGSHRLSQQ GAITKRMTAI EEMAGMDVLC SDKTGTLTLN KLSVDRNLVE VFAKGVDKEY 

       370        380        390        400        410        420 
VLLLAARASR VENQDAIDAC MVGMLADPKE ARAGIREVHF LPFNPVDKRT ALTYIDNNNN 

       430        440        450        460        470        480 
WHRASKGAPE QILDLCNAKE DVRRKVHSMM DKYAERGLRS LAVARRTVPE KSKESPGGRW 

       490        500        510        520        530        540 
EFVGLLPLFD PPRHDSAETI RRALNLGVNV KMITGDQLAI AKETGRRLGM GTNMYPSASL 

       550        560        570        580        590        600 
LGQDKDSAIA SLPIEELIEK ADGFAGVFPE HKYEIVKKLQ ERKHIVGMTG DGVNDAPALK 

       610        620        630        640        650        660 
KADIGIAVAD ATDAARGASD IVLTEPGLSV IISAVLTSRA IFQRMKNYTI YAVSITIRIV 

       670        680        690        700        710        720 
FGFMFIALIW KYDFSAFMVL IIAILNDGTI MTISKDRVKP SPMPDSWKLK EIFATGVVLG 

       730        740        750        760        770        780 
GYQALMTVVF FWAMHDTDFF SDKFGVKSLR NSDEEMMSAL YLQVSIISQA LIFVTRSRSW 

       790        800        810        820        830        840 
SFLERPGMLL VIAFMIAQLV ATLIAVYANW AFARVKGCGW GWAGVIWLYS IIFYLPLDIM 

       850        860        870        880        890        900 
KFAIRYILSG KAWNNLLDNK TAFTTKKDYG KEEREAQWAL AQRTLHGLQP PEATNLFNEK 

       910        920        930        940        950 
NSYRELSEIA EQAKRRAEMA RLRELHTLKG HVESVVKLKG LDIETIQQHY TV

« Hide

References

[1]"Identification and characterization of a second plasma membrane H(+)-ATPase gene subfamily in Nicotiana plumbaginifolia."
Moriau L., Bogaerts P., Jonniaux J.-L., Boutry M.
Plant Mol. Biol. 21:955-963(1993) [PubMed: 8490141] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Leaf.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X66737 mRNA. Translation: CAA47275.1.
PIRS33548.

3D structure databases

ProteinModelPortalQ03194.
SMRQ03194. Positions 15-847, 902-952.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR008250. ATPase_P-typ_ATPase-assoc-dom.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023300. ATPase_P-typ_cyto_domA.
IPR023299. ATPase_P-typ_cyto_domN.
IPR000695. ATPase_P-typ_H-transp.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR006534. ATPase_P-typ_PM_proton-efflux.
IPR023298. ATPase_P-typ_TM_dom.
IPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
[Graphical view]
Gene3DG3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 2 hits.
G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit.
G3DSA:1.20.1110.10. ATPase_P-typ_TM_dom. 3 hits.
PANTHERPTHR24093:SF61. PTHR24093:SF61. 1 hit.
PfamPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00120. HATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. HAD-like_dom. 1 hit.
TIGRFAMsTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePMA4_NICPL
AccessionPrimary (citable) accession number: Q03194
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 19, 2011
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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