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Protein

Trefoil factor 3

Gene

Tff3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen) (By similarity).By similarity

GO - Biological processi

  • response to peptide hormone Source: RGD
  • wound healing Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Trefoil factor 3
Alternative name(s):
Intestinal trefoil factor
Short name:
rITF
Polypeptide P1.B
Short name:
rP1.B
Gene namesi
Name:Tff3
Synonyms:Itf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3847. Tff3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 8159Trefoil factor 3PRO_0000023467Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 59PROSITE-ProRule annotation
Disulfide bondi43 ↔ 58PROSITE-ProRule annotation
Disulfide bondi53 ↔ 70PROSITE-ProRule annotation
Disulfide bondi79 – 79InterchainPROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ03191.

Expressioni

Tissue specificityi

Expressed in goblet cells of the intestines, and colon, in paraventricular hypothalamus and supraoptic nuclei. Weakly expressed in gastric epithelial cells (at protein level). Expressed by goblet cells of small and large intestinal epithelia, kidney and stomach. Expressed in the paraventricular hypothalamus, arcuate nucleus and amygdala of the brain. Weakly expressed in gastric epithelial cells.5 Publications

Inductioni

Up-regulated by hypoxia in gastric epithelial cells. Up-regulated by hypoxia-inducible factor 1 alpha (HIF1A).1 Publication

Interactioni

Subunit structurei

Monomer. Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000060620.

Structurei

3D structure databases

ProteinModelPortaliQ03191.
SMRiQ03191. Positions 23-81.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 7444P-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 P-type (trefoil) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J0FX. Eukaryota.
ENOG41114KR. LUCA.
HOGENOMiHOG000121778.
HOVERGENiHBG004364.
InParanoidiQ03191.
OrthoDBiEOG70W3GT.
PhylomeDBiQ03191.
TreeFamiTF336092.

Family and domain databases

Gene3Di4.10.110.10. 1 hit.
InterProiIPR017994. P_trefoil_chordata.
IPR017957. P_trefoil_CS.
IPR000519. P_trefoil_dom.
[Graphical view]
PfamiPF00088. Trefoil. 1 hit.
[Graphical view]
PRINTSiPR00680. PTREFOIL.
SMARTiSM00018. PD. 1 hit.
[Graphical view]
SUPFAMiSSF57492. SSF57492. 1 hit.
PROSITEiPS00025. P_TREFOIL_1. 1 hit.
PS51448. P_TREFOIL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03191-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METRAFWTTL LLVLVAGSSC KAQEFVGLSP SQCMVPANVR VDCGYPTVTS
60 70 80
EQCNNRGCCF DSSIPNVPWC FKPLQETECT F
Length:81
Mass (Da):8,944
Last modified:October 1, 1994 - v1
Checksum:i915052312C7B517E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81T → I in AAC52439 (PubMed:8750886).Curated
Sequence conflicti8 – 81T → I in AAH78873 (PubMed:15489334).Curated
Sequence conflicti8 – 81T → I in AAB01063 (PubMed:7721858).Curated
Sequence conflicti35 – 351V → A in AAA42270 (PubMed:1763017).Curated
Sequence conflicti37 – 371A → T in AAA42270 (PubMed:1763017).Curated
Sequence conflicti44 – 441G → N in AAA42270 (PubMed:1763017).Curated
Sequence conflicti81 – 811F → LDPA in AAB24079 (PubMed:1439565).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80826 mRNA. Translation: AAA42270.1.
S49317 mRNA. Translation: AAB24079.2.
U48825 mRNA. Translation: AAC52439.1.
BC078873 mRNA. Translation: AAH78873.1.
X66956 mRNA. Translation: CAA47378.1.
U20984 Genomic DNA. Translation: AAB01063.1.
AF012534 mRNA. Translation: AAB71352.1.
PIRiA41441.
A56366.
JC5623.
S23963.
RefSeqiNP_037174.2. NM_013042.2.
UniGeneiRn.87477.

Genome annotation databases

GeneIDi25563.
KEGGirno:25563.
UCSCiRGD:3847. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80826 mRNA. Translation: AAA42270.1.
S49317 mRNA. Translation: AAB24079.2.
U48825 mRNA. Translation: AAC52439.1.
BC078873 mRNA. Translation: AAH78873.1.
X66956 mRNA. Translation: CAA47378.1.
U20984 Genomic DNA. Translation: AAB01063.1.
AF012534 mRNA. Translation: AAB71352.1.
PIRiA41441.
A56366.
JC5623.
S23963.
RefSeqiNP_037174.2. NM_013042.2.
UniGeneiRn.87477.

3D structure databases

ProteinModelPortaliQ03191.
SMRiQ03191. Positions 23-81.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000060620.

Proteomic databases

PaxDbiQ03191.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25563.
KEGGirno:25563.
UCSCiRGD:3847. rat.

Organism-specific databases

CTDi7033.
RGDi3847. Tff3.

Phylogenomic databases

eggNOGiENOG410J0FX. Eukaryota.
ENOG41114KR. LUCA.
HOGENOMiHOG000121778.
HOVERGENiHBG004364.
InParanoidiQ03191.
OrthoDBiEOG70W3GT.
PhylomeDBiQ03191.
TreeFamiTF336092.

Miscellaneous databases

PROiQ03191.

Family and domain databases

Gene3Di4.10.110.10. 1 hit.
InterProiIPR017994. P_trefoil_chordata.
IPR017957. P_trefoil_CS.
IPR000519. P_trefoil_dom.
[Graphical view]
PfamiPF00088. Trefoil. 1 hit.
[Graphical view]
PRINTSiPR00680. PTREFOIL.
SMARTiSM00018. PD. 1 hit.
[Graphical view]
SUPFAMiSSF57492. SSF57492. 1 hit.
PROSITEiPS00025. P_TREFOIL_1. 1 hit.
PS51448. P_TREFOIL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of rat intestinal trefoil factor: tissue- and cell-specific member of the trefoil protein family."
    Suemori S., Lynch-Devaney K., Podolsky D.K.
    Proc. Natl. Acad. Sci. U.S.A. 88:11017-11021(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Intestine.
  2. "Trefoil peptide expression in intestinal adaptation and renewal."
    Poulsom R., Chinery R., Sarraf C., Lalani E.N., Stamp G., Elia G., Wright N.
    Scand. J. Gastroenterol. Suppl. 192:17-28(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Molecular and cellular analysis of rP1.B in the rat hypothalamus: in situ hybridization and immunohistochemistry of a new P-domain neuropeptide."
    Probst J.C., Skutella T., Mueller-Schmid A., Jirikowski G.F., Hoffmann W.
    Brain Res. Mol. Brain Res. 33:269-276(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Wistar.
    Tissue: Brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  5. "Localization of intestinal trefoil-factor mRNA in rat stomach and intestine by hybridization in situ."
    Chinery R., Poulson R., Rogers L.A., Jeffery R.E., Longcroft J.M., Hanby A.M., Wright N.A.
    Biochem. J. 285:5-8(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-80, TISSUE SPECIFICITY.
    Strain: Wistar.
    Tissue: Ileum.
  6. "Molecular cloning of the rat intestinal trefoil factor gene. Characterization of an intestinal goblet cell-associated promoter."
    Sands B.E., Ogata H., Lynch-Devaney K., Debeaumont M., Ezzell R.M., Podolsky D.K.
    J. Biol. Chem. 270:9353-9361(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
    Strain: Sprague-Dawley.
  7. "Characterization of a putative receptor for intestinal trefoil factor in rat small intestine: identification by in situ binding and ligand blotting."
    Tan X.D., Hsueh W., Chang H., Wei K.-R., Gonzalez-Crussi F.
    Biochem. Biophys. Res. Commun. 237:673-677(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 22-81, SUBCELLULAR LOCATION.
    Strain: Sprague-Dawley.
    Tissue: Small intestine.
  8. "Identification of human intestinal trefoil factor. Goblet cell-specific expression of a peptide targeted for apical secretion."
    Podolsky D.K., Lynch-Devaney K., Stow J.L., Oates P., Murgue B., Debeaumont M., Sands B.E., Mahida Y.R.
    J. Biol. Chem. 268:6694-6702(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  9. "Induction of trefoil factor (TFF)1, TFF2 and TFF3 by hypoxia is mediated by hypoxia inducible factor-1: implications for gastric mucosal healing."
    Hernandez C., Santamatilde E., McCreath K.J., Cervera A.M., Diez I., Ortiz-Masia D., Martinez N., Calatayud S., Esplugues J.V., Barrachina M.D.
    Br. J. Pharmacol. 156:262-272(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiTFF3_RAT
AccessioniPrimary (citable) accession number: Q03191
Secondary accession number(s): Q68FZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 8, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.