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Protein

Centromere protein C

Gene

CENPC

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and DNMT3B to both centromeric and pericentromeric satellite repeats and regulates the histone code in these regions.2 Publications

GO - Molecular functioni

  • centromeric DNA binding Source: InterPro
  • DNA binding Source: ProtInc

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • CENP-A containing nucleosome assembly Source: Reactome
  • chromosome segregation Source: UniProtKB
  • kinetochore assembly Source: UniProtKB
  • mitotic cell cycle Source: UniProtKB
  • mitotic nuclear division Source: UniProtKB-KW
  • sister chromatid cohesion Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-606279. Deposition of new CENPA-containing nucleosomes at the centromere.
R-HSA-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein C
Short name:
CENP-C
Alternative name(s):
Centromere autoantigen C
Centromere protein C 1
Short name:
CENP-C 1
Interphase centromere complex protein 7
Gene namesi
Name:CENPC
Synonyms:CENPC1, ICEN7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:1854. CENPC.

Subcellular locationi

GO - Cellular componenti

  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • condensed nuclear chromosome, centromeric region Source: BHF-UCL
  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • kinetochore Source: UniProtKB
  • nucleoplasm Source: Reactome
  • nucleus Source: HPA
  • pericentric heterochromatin Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26398.

Polymorphism and mutation databases

BioMutaiCENPC.
DMDMi296434446.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 943943Centromere protein CPRO_0000089474Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei73 – 731PhosphoserineCombined sources
Modified residuei96 – 961PhosphoserineCombined sources
Modified residuei130 – 1301PhosphothreonineCombined sources
Modified residuei146 – 1461PhosphoserineCombined sources
Modified residuei183 – 1831PhosphothreonineCombined sources
Modified residuei189 – 1891PhosphoserineCombined sources
Cross-linki217 – 217Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei225 – 2251PhosphoserineCombined sources
Modified residuei261 – 2611PhosphoserineCombined sources
Modified residuei316 – 3161PhosphoserineCombined sources
Modified residuei333 – 3331Phosphoserine; in variant Phe-341Combined sources
Modified residuei376 – 3761PhosphoserineBy similarity
Modified residuei397 – 3971PhosphoserineBy similarity
Modified residuei439 – 4391PhosphoserineCombined sources
Modified residuei528 – 5281PhosphoserineCombined sources
Modified residuei538 – 5381PhosphoserineCombined sources
Modified residuei684 – 6841PhosphoserineCombined sources
Modified residuei709 – 7091PhosphoserineCombined sources
Modified residuei710 – 7101PhosphoserineCombined sources
Modified residuei734 – 7341PhosphothreonineCombined sources
Modified residuei763 – 7631PhosphoserineCombined sources
Modified residuei773 – 7731PhosphoserineCombined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ03188.
MaxQBiQ03188.
PaxDbiQ03188.
PeptideAtlasiQ03188.
PRIDEiQ03188.

PTM databases

iPTMnetiQ03188.
PhosphoSiteiQ03188.

Expressioni

Gene expression databases

BgeeiENSG00000145241.
CleanExiHS_CENPC1.
ExpressionAtlasiQ03188. baseline and differential.
GenevisibleiQ03188. HS.

Organism-specific databases

HPAiHPA049740.
HPA058252.

Interactioni

Subunit structurei

Oligomer. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Binds to DAXX. Interacts directly with CENPA. Interacts with MEIKIN (By similarity).By similarity3 Publications

Protein-protein interaction databases

BioGridi107489. 34 interactions.
IntActiQ03188. 24 interactions.
MINTiMINT-4786577.
STRINGi9606.ENSP00000273853.

Structurei

3D structure databases

ProteinModelPortaliQ03188.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni737 – 75923MIF2 homology domain IIAdd
BLAST
Regioni890 – 94354MIF2 homology domain IIIAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi259 – 27315Nuclear localization signalSequence analysisAdd
BLAST
Motifi484 – 49916Nuclear localization signalSequence analysisAdd
BLAST
Motifi558 – 57417Nuclear localization signalSequence analysisAdd
BLAST
Motifi780 – 79819Nuclear localization signalSequence analysisAdd
BLAST

Domaini

The MIF2 homology domain II targets centromeres and binds the alpha satellite DNA in vivo. The MIF2 homology domain III can induce CENPC dimerization/oligomerization.1 Publication

Sequence similaritiesi

Belongs to the CENPC family.Curated

Phylogenomic databases

eggNOGiENOG410IEH1. Eukaryota.
ENOG41123PF. LUCA.
GeneTreeiENSGT00390000016737.
HOGENOMiHOG000090256.
HOVERGENiHBG050891.
InParanoidiQ03188.
KOiK11497.
OMAiDWWVVKS.
OrthoDBiEOG091G03MG.
PhylomeDBiQ03188.
TreeFamiTF101132.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR028386. CENP-C/Mif2/cnp3.
IPR028931. CENP-C_mid.
IPR028052. CENP_C_N_dom.
IPR025974. Mif2/CENP-C_cupin.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR16684. PTHR16684. 1 hit.
PfamiPF11699. CENP-C_C. 1 hit.
PF15620. CENP-C_mid. 1 hit.
PF15622. CENP_C_N. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q03188-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASGLDHLK NGYRRRFCRP SRARDINTEQ GQNVLEILQD CFEEKSLAND
60 70 80 90 100
FSTNSTKSVP NSTRKIKDTC IQSPSKECQK SHPKSVPVSS KKKEASLQFV
110 120 130 140 150
VEPSEATNRS VQAHEVHQKI LATDVSSKNT PDSKKISSRN INDHHSEADE
160 170 180 190 200
EFYLSVGSPS VLLDAKTSVS QNVIPSSAQK RETYTFENSV NMLPSSTEVS
210 220 230 240 250
VKTKKRLNFD DKVMLKKIEI DNKVSDEEDK TSEGQERKPS GSSQNRIRDS
260 270 280 290 300
EYEIQRQAKK SFSTLFLETV KRKSESSPIV RHAATAPPHS CPPDDTKLIE
310 320 330 340 350
DEFIIDESDQ SFASRSWITI PRKAGSLKQR TISPAESTAL LQGRKSREKH
360 370 380 390 400
HNILPKTLAN DKHSHKPHPV ETSQPSDKTV LDTSYALIGE TVNNYRSTKY
410 420 430 440 450
EMYSKNAEKP SRSKRTIKQK QRRKFMAKPA EEQLDVGQSK DENIHTSHIT
460 470 480 490 500
QDEFQRNSDR NMEEHEEMGN DCVSKKQMPP VGSKKSSTRK DKEESKKKRF
510 520 530 540 550
SSESKNKLVP EEVTSTVTKS RRISRRPSDW WVVKSEESPV YSNSSVRNEL
560 570 580 590 600
PMHHNSSRKS TKKTNQSSKN IRKKTIPLKR QKTATKGNQR VQKFLNAEGS
610 620 630 640 650
GGIVGHDEIS RCSLSEPLES DEADLAKKKN LDCSRSTRSS KNEDNIMTAQ
660 670 680 690 700
NVPLKPQTSG YTCNIPTESN LDSGEHKTSV LEESGPSRLN NNYLMSGKND
710 720 730 740 750
VDDEEVHGSS DDSKQSKVIP KNRIHHKLVL PSNTPNVRRT KRTRLKPLEY
760 770 780 790 800
WRGERIDYQG RPSGGFVISG VLSPDTISSK RKAKENIGKV NKKSNKKRIC
810 820 830 840 850
LDNDERKTNL MVNLGIPLGD PLQPTRVKDP ETREIILMDL VRPQDTYQFF
860 870 880 890 900
VKHGELKVYK TLDTPFFSTG KLILGPQEEK GKQHVGQDIL VFYVNFGDLL
910 920 930 940
CTLHETPYIL STGDSFYVPS GNYYNIKNLR NEESVLLFTQ IKR
Length:943
Mass (Da):106,834
Last modified:May 18, 2010 - v2
Checksum:i2AA5AF82BA88C809
GO
Isoform 2 (identifier: Q03188-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     539-542: PVYS → CLKC
     543-943: Missing.

Note: No experimental confirmation available.
Show »
Length:542
Mass (Da):61,544
Checksum:iF5ADBF8C360F6382
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti179 – 1791Q → K in AAA51974 (PubMed:1339310).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti341 – 3411L → F.Combined sources3 Publications
Corresponds to variant rs11250 [ dbSNP | Ensembl ].
VAR_069295
Natural varianti389 – 3891G → D.1 Publication
Corresponds to variant rs1056787 [ dbSNP | Ensembl ].
VAR_069296

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei539 – 5424PVYS → CLKC in isoform 2. 1 PublicationVSP_057280
Alternative sequencei543 – 943401Missing in isoform 2. 1 PublicationVSP_057281Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95724 mRNA. Translation: AAA51974.1.
AC104806 Genomic DNA. No translation available.
AC109356 Genomic DNA. No translation available.
CH471057 Genomic DNA. Translation: EAX05545.1.
BC030695 mRNA. Translation: AAH30695.1.
BC041117 mRNA. Translation: AAH41117.1.
AF151723 Genomic DNA. Translation: AAF73191.1.
CCDSiCCDS47063.1. [Q03188-1]
PIRiA42681.
RefSeqiNP_001803.2. NM_001812.2. [Q03188-1]
UniGeneiHs.479867.

Genome annotation databases

EnsembliENST00000273853; ENSP00000273853; ENSG00000145241. [Q03188-1]
ENST00000506882; ENSP00000426078; ENSG00000145241. [Q03188-2]
GeneIDi1060.
KEGGihsa:1060.
UCSCiuc003hdd.2. human. [Q03188-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95724 mRNA. Translation: AAA51974.1.
AC104806 Genomic DNA. No translation available.
AC109356 Genomic DNA. No translation available.
CH471057 Genomic DNA. Translation: EAX05545.1.
BC030695 mRNA. Translation: AAH30695.1.
BC041117 mRNA. Translation: AAH41117.1.
AF151723 Genomic DNA. Translation: AAF73191.1.
CCDSiCCDS47063.1. [Q03188-1]
PIRiA42681.
RefSeqiNP_001803.2. NM_001812.2. [Q03188-1]
UniGeneiHs.479867.

3D structure databases

ProteinModelPortaliQ03188.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107489. 34 interactions.
IntActiQ03188. 24 interactions.
MINTiMINT-4786577.
STRINGi9606.ENSP00000273853.

PTM databases

iPTMnetiQ03188.
PhosphoSiteiQ03188.

Polymorphism and mutation databases

BioMutaiCENPC.
DMDMi296434446.

Proteomic databases

EPDiQ03188.
MaxQBiQ03188.
PaxDbiQ03188.
PeptideAtlasiQ03188.
PRIDEiQ03188.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000273853; ENSP00000273853; ENSG00000145241. [Q03188-1]
ENST00000506882; ENSP00000426078; ENSG00000145241. [Q03188-2]
GeneIDi1060.
KEGGihsa:1060.
UCSCiuc003hdd.2. human. [Q03188-1]

Organism-specific databases

CTDi1060.
GeneCardsiCENPC.
H-InvDBHIX0024603.
HGNCiHGNC:1854. CENPC.
HPAiHPA049740.
HPA058252.
MIMi117141. gene.
neXtProtiNX_Q03188.
PharmGKBiPA26398.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEH1. Eukaryota.
ENOG41123PF. LUCA.
GeneTreeiENSGT00390000016737.
HOGENOMiHOG000090256.
HOVERGENiHBG050891.
InParanoidiQ03188.
KOiK11497.
OMAiDWWVVKS.
OrthoDBiEOG091G03MG.
PhylomeDBiQ03188.
TreeFamiTF101132.

Enzyme and pathway databases

ReactomeiR-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-606279. Deposition of new CENPA-containing nucleosomes at the centromere.
R-HSA-68877. Mitotic Prometaphase.

Miscellaneous databases

GeneWikiiCENPC1.
GenomeRNAii1060.
PROiQ03188.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000145241.
CleanExiHS_CENPC1.
ExpressionAtlasiQ03188. baseline and differential.
GenevisibleiQ03188. HS.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR028386. CENP-C/Mif2/cnp3.
IPR028931. CENP-C_mid.
IPR028052. CENP_C_N_dom.
IPR025974. Mif2/CENP-C_cupin.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR16684. PTHR16684. 1 hit.
PfamiPF11699. CENP-C_C. 1 hit.
PF15620. CENP-C_mid. 1 hit.
PF15622. CENP_C_N. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCENPC_HUMAN
AccessioniPrimary (citable) accession number: Q03188
Secondary accession number(s): Q6PIR0, Q8IW27, Q9P0M5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: May 18, 2010
Last modified: September 7, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.