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Protein

Centromere protein C

Gene

CENPC

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and DNMT3B to both centromeric and pericentromeric satellite repeats and regulates the histone code in these regions.2 Publications

GO - Molecular functioni

  • centromeric DNA binding Source: GO_Central
  • DNA binding Source: ProtInc

GO - Biological processi

  • attachment of mitotic spindle microtubules to kinetochore Source: GO_Central
  • attachment of spindle microtubules to kinetochore involved in homologous chromosome segregation Source: GO_Central
  • cell division Source: UniProtKB-KW
  • CENP-A containing nucleosome assembly Source: Reactome
  • chromosome segregation Source: UniProtKB
  • kinetochore assembly Source: UniProtKB
  • mitotic cell cycle Source: UniProtKB
  • sister chromatid cohesion Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000145241-MONOMER.
ReactomeiR-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-606279. Deposition of new CENPA-containing nucleosomes at the centromere.
R-HSA-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein C
Short name:
CENP-C
Alternative name(s):
Centromere autoantigen C
Centromere protein C 1
Short name:
CENP-C 1
Interphase centromere complex protein 7
Gene namesi
Name:CENPC
Synonyms:CENPC1, ICEN7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:1854. CENPC.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome, centromeric region Source: BHF-UCL
  • condensed nuclear chromosome kinetochore Source: GO_Central
  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • kinetochore Source: UniProtKB
  • nucleoplasm Source: Reactome
  • nucleus Source: HPA
  • pericentric heterochromatin Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1060.
OpenTargetsiENSG00000145241.
PharmGKBiPA26398.

Polymorphism and mutation databases

BioMutaiCENPC.
DMDMi296434446.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000894741 – 943Centromere protein CAdd BLAST943

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei73PhosphoserineCombined sources1
Modified residuei96PhosphoserineCombined sources1
Modified residuei130PhosphothreonineCombined sources1
Modified residuei146PhosphoserineCombined sources1
Modified residuei183PhosphothreonineCombined sources1
Modified residuei189PhosphoserineCombined sources1
Cross-linki217Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei225PhosphoserineCombined sources1
Modified residuei261PhosphoserineCombined sources1
Modified residuei316PhosphoserineCombined sources1
Modified residuei333PhosphoserineCombined sources1
Modified residuei376PhosphoserineBy similarity1
Modified residuei397PhosphoserineBy similarity1
Modified residuei439PhosphoserineCombined sources1
Modified residuei528PhosphoserineCombined sources1
Modified residuei538PhosphoserineCombined sources1
Modified residuei684PhosphoserineCombined sources1
Modified residuei709PhosphoserineCombined sources1
Modified residuei710PhosphoserineCombined sources1
Modified residuei734PhosphothreonineCombined sources1
Modified residuei763PhosphoserineCombined sources1
Modified residuei773PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ03188.
MaxQBiQ03188.
PaxDbiQ03188.
PeptideAtlasiQ03188.
PRIDEiQ03188.

PTM databases

iPTMnetiQ03188.
PhosphoSitePlusiQ03188.

Expressioni

Gene expression databases

BgeeiENSG00000145241.
CleanExiHS_CENPC1.
ExpressionAtlasiQ03188. baseline and differential.
GenevisibleiQ03188. HS.

Organism-specific databases

HPAiHPA049740.
HPA058252.

Interactioni

Subunit structurei

Oligomer. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU (PubMed:16622419). The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Binds to DAXX (PubMed:9645950). Interacts with DNMT3B (PubMed:19482874). Interacts directly with CENPA (PubMed:19503796). Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1 (PubMed:27499292). Interacts with MEIKIN (By similarity).By similarity5 Publications

Protein-protein interaction databases

BioGridi107489. 34 interactors.
IntActiQ03188. 24 interactors.
MINTiMINT-4786577.
STRINGi9606.ENSP00000273853.

Structurei

3D structure databases

ProteinModelPortaliQ03188.
SMRiQ03188.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni737 – 759MIF2 homology domain IIAdd BLAST23
Regioni890 – 943MIF2 homology domain IIIAdd BLAST54

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi259 – 273Nuclear localization signalSequence analysisAdd BLAST15
Motifi484 – 499Nuclear localization signalSequence analysisAdd BLAST16
Motifi558 – 574Nuclear localization signalSequence analysisAdd BLAST17
Motifi780 – 798Nuclear localization signalSequence analysisAdd BLAST19

Domaini

The MIF2 homology domain II targets centromeres and binds the alpha satellite DNA in vivo. The MIF2 homology domain III can induce CENPC dimerization/oligomerization.1 Publication

Sequence similaritiesi

Belongs to the CENPC family.Curated

Phylogenomic databases

eggNOGiENOG410IEH1. Eukaryota.
ENOG41123PF. LUCA.
GeneTreeiENSGT00390000016737.
HOGENOMiHOG000090256.
HOVERGENiHBG050891.
InParanoidiQ03188.
KOiK11497.
OMAiDWWVVKS.
OrthoDBiEOG091G03MG.
PhylomeDBiQ03188.
TreeFamiTF101132.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR028386. CENP-C/Mif2/cnp3.
IPR028931. CENP-C_mid.
IPR028052. CENP_C_N_dom.
IPR025974. Mif2/CENP-C_cupin.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR16684. PTHR16684. 1 hit.
PfamiPF11699. CENP-C_C. 1 hit.
PF15620. CENP-C_mid. 1 hit.
PF15622. CENP_C_N. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q03188-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASGLDHLK NGYRRRFCRP SRARDINTEQ GQNVLEILQD CFEEKSLAND
60 70 80 90 100
FSTNSTKSVP NSTRKIKDTC IQSPSKECQK SHPKSVPVSS KKKEASLQFV
110 120 130 140 150
VEPSEATNRS VQAHEVHQKI LATDVSSKNT PDSKKISSRN INDHHSEADE
160 170 180 190 200
EFYLSVGSPS VLLDAKTSVS QNVIPSSAQK RETYTFENSV NMLPSSTEVS
210 220 230 240 250
VKTKKRLNFD DKVMLKKIEI DNKVSDEEDK TSEGQERKPS GSSQNRIRDS
260 270 280 290 300
EYEIQRQAKK SFSTLFLETV KRKSESSPIV RHAATAPPHS CPPDDTKLIE
310 320 330 340 350
DEFIIDESDQ SFASRSWITI PRKAGSLKQR TISPAESTAL LQGRKSREKH
360 370 380 390 400
HNILPKTLAN DKHSHKPHPV ETSQPSDKTV LDTSYALIGE TVNNYRSTKY
410 420 430 440 450
EMYSKNAEKP SRSKRTIKQK QRRKFMAKPA EEQLDVGQSK DENIHTSHIT
460 470 480 490 500
QDEFQRNSDR NMEEHEEMGN DCVSKKQMPP VGSKKSSTRK DKEESKKKRF
510 520 530 540 550
SSESKNKLVP EEVTSTVTKS RRISRRPSDW WVVKSEESPV YSNSSVRNEL
560 570 580 590 600
PMHHNSSRKS TKKTNQSSKN IRKKTIPLKR QKTATKGNQR VQKFLNAEGS
610 620 630 640 650
GGIVGHDEIS RCSLSEPLES DEADLAKKKN LDCSRSTRSS KNEDNIMTAQ
660 670 680 690 700
NVPLKPQTSG YTCNIPTESN LDSGEHKTSV LEESGPSRLN NNYLMSGKND
710 720 730 740 750
VDDEEVHGSS DDSKQSKVIP KNRIHHKLVL PSNTPNVRRT KRTRLKPLEY
760 770 780 790 800
WRGERIDYQG RPSGGFVISG VLSPDTISSK RKAKENIGKV NKKSNKKRIC
810 820 830 840 850
LDNDERKTNL MVNLGIPLGD PLQPTRVKDP ETREIILMDL VRPQDTYQFF
860 870 880 890 900
VKHGELKVYK TLDTPFFSTG KLILGPQEEK GKQHVGQDIL VFYVNFGDLL
910 920 930 940
CTLHETPYIL STGDSFYVPS GNYYNIKNLR NEESVLLFTQ IKR
Length:943
Mass (Da):106,834
Last modified:May 18, 2010 - v2
Checksum:i2AA5AF82BA88C809
GO
Isoform 2 (identifier: Q03188-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     539-542: PVYS → CLKC
     543-943: Missing.

Note: No experimental confirmation available.
Show »
Length:542
Mass (Da):61,544
Checksum:iF5ADBF8C360F6382
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti179Q → K in AAA51974 (PubMed:1339310).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_069295341L → F.Combined sources3 PublicationsCorresponds to variant rs11250dbSNPEnsembl.1
Natural variantiVAR_069296389G → D.1 PublicationCorresponds to variant rs1056787dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_057280539 – 542PVYS → CLKC in isoform 2. 1 Publication4
Alternative sequenceiVSP_057281543 – 943Missing in isoform 2. 1 PublicationAdd BLAST401

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95724 mRNA. Translation: AAA51974.1.
AC104806 Genomic DNA. No translation available.
AC109356 Genomic DNA. No translation available.
CH471057 Genomic DNA. Translation: EAX05545.1.
BC030695 mRNA. Translation: AAH30695.1.
BC041117 mRNA. Translation: AAH41117.1.
AF151723 Genomic DNA. Translation: AAF73191.1.
CCDSiCCDS47063.1. [Q03188-1]
PIRiA42681.
RefSeqiNP_001803.2. NM_001812.2. [Q03188-1]
UniGeneiHs.479867.

Genome annotation databases

EnsembliENST00000273853; ENSP00000273853; ENSG00000145241. [Q03188-1]
ENST00000506882; ENSP00000426078; ENSG00000145241. [Q03188-2]
GeneIDi1060.
KEGGihsa:1060.
UCSCiuc003hdd.2. human. [Q03188-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95724 mRNA. Translation: AAA51974.1.
AC104806 Genomic DNA. No translation available.
AC109356 Genomic DNA. No translation available.
CH471057 Genomic DNA. Translation: EAX05545.1.
BC030695 mRNA. Translation: AAH30695.1.
BC041117 mRNA. Translation: AAH41117.1.
AF151723 Genomic DNA. Translation: AAF73191.1.
CCDSiCCDS47063.1. [Q03188-1]
PIRiA42681.
RefSeqiNP_001803.2. NM_001812.2. [Q03188-1]
UniGeneiHs.479867.

3D structure databases

ProteinModelPortaliQ03188.
SMRiQ03188.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107489. 34 interactors.
IntActiQ03188. 24 interactors.
MINTiMINT-4786577.
STRINGi9606.ENSP00000273853.

PTM databases

iPTMnetiQ03188.
PhosphoSitePlusiQ03188.

Polymorphism and mutation databases

BioMutaiCENPC.
DMDMi296434446.

Proteomic databases

EPDiQ03188.
MaxQBiQ03188.
PaxDbiQ03188.
PeptideAtlasiQ03188.
PRIDEiQ03188.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000273853; ENSP00000273853; ENSG00000145241. [Q03188-1]
ENST00000506882; ENSP00000426078; ENSG00000145241. [Q03188-2]
GeneIDi1060.
KEGGihsa:1060.
UCSCiuc003hdd.2. human. [Q03188-1]

Organism-specific databases

CTDi1060.
DisGeNETi1060.
GeneCardsiCENPC.
H-InvDBHIX0024603.
HGNCiHGNC:1854. CENPC.
HPAiHPA049740.
HPA058252.
MIMi117141. gene.
neXtProtiNX_Q03188.
OpenTargetsiENSG00000145241.
PharmGKBiPA26398.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEH1. Eukaryota.
ENOG41123PF. LUCA.
GeneTreeiENSGT00390000016737.
HOGENOMiHOG000090256.
HOVERGENiHBG050891.
InParanoidiQ03188.
KOiK11497.
OMAiDWWVVKS.
OrthoDBiEOG091G03MG.
PhylomeDBiQ03188.
TreeFamiTF101132.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000145241-MONOMER.
ReactomeiR-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-606279. Deposition of new CENPA-containing nucleosomes at the centromere.
R-HSA-68877. Mitotic Prometaphase.

Miscellaneous databases

GeneWikiiCENPC1.
GenomeRNAii1060.
PROiQ03188.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000145241.
CleanExiHS_CENPC1.
ExpressionAtlasiQ03188. baseline and differential.
GenevisibleiQ03188. HS.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR028386. CENP-C/Mif2/cnp3.
IPR028931. CENP-C_mid.
IPR028052. CENP_C_N_dom.
IPR025974. Mif2/CENP-C_cupin.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR16684. PTHR16684. 1 hit.
PfamiPF11699. CENP-C_C. 1 hit.
PF15620. CENP-C_mid. 1 hit.
PF15622. CENP_C_N. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCENPC_HUMAN
AccessioniPrimary (citable) accession number: Q03188
Secondary accession number(s): Q6PIR0, Q8IW27, Q9P0M5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: May 18, 2010
Last modified: November 30, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.