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Q03188 (CENPC_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Centromere protein C

Short name=CENP-C
Alternative name(s):
Centromere autoantigen C
Centromere protein C 1
Short name=CENP-C 1
Interphase centromere complex protein 7
Gene names
Name:CENPC
Synonyms:CENPC1, ICEN7
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length943 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and DNMT3B to both centromeric and pericentromeric satellite repeats and regulates the histone code in these regions. Ref.12 Ref.15

Subunit structure

Oligomer. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Binds to DAXX. Interacts directly with CENPA. Ref.8 Ref.11 Ref.12

Subcellular location

Nucleus. Chromosomecentromerekinetochore. Note: Localizes exclusively in the kinetochore domain of centromeres. Ref.9 Ref.12 Ref.15

Domain

The MIF2 homology domain II targets centromeres and binds the alpha satellite DNA in vivo. The MIF2 homology domain III can induce CENPC dimerization/oligomerization. Ref.11

Sequence similarities

Belongs to the CENPC family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 943943Centromere protein C
PRO_0000089474

Regions

Region737 – 75923MIF2 homology domain II
Region890 – 94354MIF2 homology domain III
Motif259 – 27315Nuclear localization signal Potential
Motif484 – 49916Nuclear localization signal Potential
Motif558 – 57417Nuclear localization signal Potential
Motif780 – 79819Nuclear localization signal Potential

Amino acid modifications

Modified residue961Phosphoserine Ref.14
Modified residue1301Phosphothreonine Ref.14
Modified residue1461Phosphoserine Ref.13
Modified residue1831Phosphothreonine Ref.13
Modified residue1891Phosphoserine Ref.13
Modified residue2251Phosphoserine Ref.6 Ref.14 Ref.16
Modified residue2611Phosphoserine Ref.10
Modified residue3161Phosphoserine Ref.10
Modified residue4391Phosphoserine Ref.14 Ref.16
Modified residue5381Phosphoserine Ref.13 Ref.14
Modified residue7091Phosphoserine Ref.16
Modified residue7101Phosphoserine Ref.16
Modified residue7341Phosphothreonine Ref.14
Modified residue7631Phosphoserine Ref.13 Ref.14
Modified residue7731Phosphoserine Ref.10 Ref.13 Ref.14

Natural variations

Natural variant3411L → F. Ref.1 Ref.3 Ref.4 Ref.10
Corresponds to variant rs11250 [ dbSNP | Ensembl ].
VAR_069295
Natural variant3891G → D. Ref.1
Corresponds to variant rs1056787 [ dbSNP | Ensembl ].
VAR_069296

Experimental info

Sequence conflict1791Q → K in AAA51974. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q03188 [UniParc].

Last modified May 18, 2010. Version 2.
Checksum: 2AA5AF82BA88C809

FASTA943106,834
        10         20         30         40         50         60 
MAASGLDHLK NGYRRRFCRP SRARDINTEQ GQNVLEILQD CFEEKSLAND FSTNSTKSVP 

        70         80         90        100        110        120 
NSTRKIKDTC IQSPSKECQK SHPKSVPVSS KKKEASLQFV VEPSEATNRS VQAHEVHQKI 

       130        140        150        160        170        180 
LATDVSSKNT PDSKKISSRN INDHHSEADE EFYLSVGSPS VLLDAKTSVS QNVIPSSAQK 

       190        200        210        220        230        240 
RETYTFENSV NMLPSSTEVS VKTKKRLNFD DKVMLKKIEI DNKVSDEEDK TSEGQERKPS 

       250        260        270        280        290        300 
GSSQNRIRDS EYEIQRQAKK SFSTLFLETV KRKSESSPIV RHAATAPPHS CPPDDTKLIE 

       310        320        330        340        350        360 
DEFIIDESDQ SFASRSWITI PRKAGSLKQR TISPAESTAL LQGRKSREKH HNILPKTLAN 

       370        380        390        400        410        420 
DKHSHKPHPV ETSQPSDKTV LDTSYALIGE TVNNYRSTKY EMYSKNAEKP SRSKRTIKQK 

       430        440        450        460        470        480 
QRRKFMAKPA EEQLDVGQSK DENIHTSHIT QDEFQRNSDR NMEEHEEMGN DCVSKKQMPP 

       490        500        510        520        530        540 
VGSKKSSTRK DKEESKKKRF SSESKNKLVP EEVTSTVTKS RRISRRPSDW WVVKSEESPV 

       550        560        570        580        590        600 
YSNSSVRNEL PMHHNSSRKS TKKTNQSSKN IRKKTIPLKR QKTATKGNQR VQKFLNAEGS 

       610        620        630        640        650        660 
GGIVGHDEIS RCSLSEPLES DEADLAKKKN LDCSRSTRSS KNEDNIMTAQ NVPLKPQTSG 

       670        680        690        700        710        720 
YTCNIPTESN LDSGEHKTSV LEESGPSRLN NNYLMSGKND VDDEEVHGSS DDSKQSKVIP 

       730        740        750        760        770        780 
KNRIHHKLVL PSNTPNVRRT KRTRLKPLEY WRGERIDYQG RPSGGFVISG VLSPDTISSK 

       790        800        810        820        830        840 
RKAKENIGKV NKKSNKKRIC LDNDERKTNL MVNLGIPLGD PLQPTRVKDP ETREIILMDL 

       850        860        870        880        890        900 
VRPQDTYQFF VKHGELKVYK TLDTPFFSTG KLILGPQEEK GKQHVGQDIL VFYVNFGDLL 

       910        920        930        940 
CTLHETPYIL STGDSFYVPS GNYYNIKNLR NEESVLLFTQ IKR 

« Hide

References

« Hide 'large scale' references
[1]"CENP-C, an autoantigen in scleroderma, is a component of the human inner kinetochore plate."
Saitoh H., Tomkiel J., Cooke C.A., Ratrie H. III, Maurer M., Rothfield N.F., Earnshaw W.C.
Cell 70:115-125(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS PHE-341 AND ASP-389.
Tissue: Placenta.
[2]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT PHE-341.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PHE-341.
Tissue: Blood.
[5]"Promoter characterization of centromere protein C reveals its participation in cell cycle regulation in late G1-phase and expression control by E2F-1, pRb, p107 and Sp-1."
Poppe M., Botz J., Hahm B., Dobat K., Eickelbaum W., Paweletz N., Arand M., Knehr M.
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
[6]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells."
Izuta H., Ikeno M., Suzuki N., Tomonaga T., Nozaki N., Obuse C., Kisu Y., Goshima N., Nomura F., Nomura N., Yoda K.
Genes Cells 11:673-684(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
[8]"The human CENP-A centromeric nucleosome-associated complex."
Foltz D.R., Jansen L.E.T., Black B.E., Bailey A.O., Yates J.R. III, Cleveland D.W.
Nat. Cell Biol. 8:458-469(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CENPA-NAC COMPLEX WITH CENPA; CENPH; CENPM; CENPN; CENPT AND CENPU.
[9]"Co-localization of CENP-C and CENP-H to discontinuous domains of CENP-A chromatin at human neocentromeres."
Alonso A., Fritz B., Hasson D., Abrusan G., Cheung F., Yoda K., Radlwimmer B., Ladurner A.G., Warburton P.E.
Genome Biol. 8:R148.1-R148.19(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-261; SER-316 AND SER-773, VARIANT [LARGE SCALE ANALYSIS] PHE-341, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"The C-terminal domain of CENP-C displays multiple and critical functions for mammalian centromere formation."
Trazzi S., Perini G., Bernardoni R., Zoli M., Reese J.C., Musacchio A., Della Valle G.
PLoS ONE 4:E5832-E5832(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN, SUBUNIT, INTERACTION WITH CENPA.
[12]"DNMT3B interacts with constitutive centromere protein CENP-C to modulate DNA methylation and the histone code at centromeric regions."
Gopalakrishnan S., Sullivan B.A., Trazzi S., Della Valle G., Robertson K.D.
Hum. Mol. Genet. 18:3178-3193(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH DNMT3B.
[13]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-146; THR-183; SER-189; SER-538; SER-763 AND SER-773, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[14]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96; THR-130; SER-225; SER-439; SER-538; THR-734; SER-763 AND SER-773, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[15]"Induced ectopic kinetochore assembly bypasses the requirement for CENP-A nucleosomes."
Gascoigne K.E., Takeuchi K., Suzuki A., Hori T., Fukagawa T., Cheeseman I.M.
Cell 145:410-422(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[16]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225; SER-439; SER-709 AND SER-710, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M95724 mRNA. Translation: AAA51974.1.
AC104806 Genomic DNA. No translation available.
AC109356 Genomic DNA. No translation available.
CH471057 Genomic DNA. Translation: EAX05545.1.
BC041117 mRNA. Translation: AAH41117.1.
AF151723 Genomic DNA. Translation: AAF73191.1.
CCDSCCDS47063.1.
PIRA42681.
RefSeqNP_001803.2. NM_001812.2.
UniGeneHs.479867.

3D structure databases

ProteinModelPortalQ03188.
SMRQ03188. Positions 908-936.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107489. 14 interactions.
IntActQ03188. 11 interactions.
MINTMINT-4786577.
STRING9606.ENSP00000273853.

PTM databases

PhosphoSiteQ03188.

Polymorphism databases

DMDM296434446.

Proteomic databases

MaxQBQ03188.
PaxDbQ03188.
PRIDEQ03188.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000273853; ENSP00000273853; ENSG00000145241.
GeneID1060.
KEGGhsa:1060.
UCSCuc003hdd.1. human.

Organism-specific databases

CTD1060.
GeneCardsGC04M068337.
H-InvDBHIX0024603.
HGNCHGNC:1854. CENPC.
HPAHPA049740.
MIM117141. gene.
neXtProtNX_Q03188.
PharmGKBPA26398.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG329217.
HOGENOMHOG000090256.
HOVERGENHBG050891.
InParanoidQ03188.
KOK11497.
OMAGYRRRFC.
OrthoDBEOG73FQQ8.
PhylomeDBQ03188.
TreeFamTF101132.

Enzyme and pathway databases

ReactomeREACT_115566. Cell Cycle.
REACT_21300. Mitotic M-M/G1 phases.

Gene expression databases

ArrayExpressQ03188.
BgeeQ03188.
CleanExHS_CENPC1.
GenevestigatorQ03188.

Family and domain databases

Gene3D2.60.120.10. 1 hit.
InterProIPR028386. CENP-C/Mif2/cnp3.
IPR028931. CENP-C_mid.
IPR028052. CENP_C_N_dom.
IPR025974. Mif2/CENP-C_cupin.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERPTHR16684. PTHR16684. 1 hit.
PfamPF11699. CENP-C_C. 1 hit.
PF15620. CENP-C_mid. 1 hit.
PF15622. CENP_C_N. 1 hit.
[Graphical view]
SUPFAMSSF51182. SSF51182. 1 hit.
ProtoNetSearch...

Other

ChiTaRSCENPC1. human.
GeneWikiCENPC1.
GenomeRNAi1060.
NextBio4436.
PROQ03188.
SOURCESearch...

Entry information

Entry nameCENPC_HUMAN
AccessionPrimary (citable) accession number: Q03188
Secondary accession number(s): Q8IW27, Q9P0M5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: May 18, 2010
Last modified: July 9, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM