Reviewed, UniProtKB/Swiss-Prot Q03180 (PIR3_YEAST)

Names and origin

Protein names

Recommended name:
    Cell wall mannoprotein PIR3
Alternative name(s):
    Protein with internal repeats 3
    Covalently-linked cell wall protein 8

Gene names

Name:	PIR3
Synonyms:	CCW8
Ordered Locus Names:	YKL163W
ORF Names:	YKL617

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the outer cell wall layer. Required for stability of the cell wall and for optimal growth. Required for resistance against several antifungal and cell wall-perturbing agents. Ref.6 Ref.8 Ref.10
Subcellular location	Secreted › cell wall. Note: Covalently attached to the cell wall. Ref.6 Ref.8 Ref.12
Induction	Positively regulated by signaling through MPK1 in response to cell wall perturbation. Expression is regulated by the ACE2 and SWI5 transcription factors. Ref.7 Ref.9 Ref.11 Ref.14
Domain	The PIR1/2/3 repeats are required for covalent linkage to the cell wall By similarity. Their number varies among different strains of S.cerevisiae.
Post-translational modification	Covalently linked to beta-1,3-glucan of the inner cell wall layer via an alkali-sensitive ester linkage between the gamma-carboxyl group of glutamic acids, arising from specific glutamines within the PIR1/2/3 repeats, and hydroxyl groups of glucoses of beta-1,3-glucan chains By similarity. O-glycosylated. Extensively O-mannosylated. Ref.4
Sequence similarities	Belongs to the PIR protein family. Contains 8 PIR1/2/3 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Repeat Signal
PTM	Cleavage on pair of basic residues Glycoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall organization Ref.8 Inferred from mutant phenotype. Source: SGD
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW fungal-type cell wall Ref.6 Ref.8 Ref.12 Inferred from direct assay. Source: SGD
Molecular function	structural constituent of cell wall Ref.8 Inferred from physical interaction. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.4
	Propeptide	19 – 67	49			PRO_0000033256
	Chain	68 – 325	258	Cell wall mannoprotein PIR3		PRO_0000033257
Regions
	Repeat	68 – 91	24	PIR1/2/3 1
	Repeat	92 – 109	18	PIR1/2/3 2
	Repeat	110 – 127	18	PIR1/2/3 3
	Repeat	128 – 145	18	PIR1/2/3 4
	Repeat	146 – 163	18	PIR1/2/3 5
	Repeat	164 – 181	18	PIR1/2/3 6
	Repeat	182 – 199	18	PIR1/2/3 7
	Repeat	200 – 217	18	PIR1/2/3 8
Sites
	Site	67 – 68	2	Cleavage; by KEX2
	Site	76	1	Covalent attachment to cell wall glycan By similarity
	Site	102	1	Covalent attachment to cell wall glycan By similarity
	Site	120	1	Covalent attachment to cell wall glycan By similarity
	Site	138	1	Covalent attachment to cell wall glycan By similarity
	Site	156	1	Covalent attachment to cell wall glycan By similarity
	Site	174	1	Covalent attachment to cell wall glycan By similarity
	Site	192	1	Covalent attachment to cell wall glycan By similarity
	Site	209	1	Covalent attachment to cell wall glycan By similarity
Natural variations
	Natural variant	113	1	V → A in strain: RAY-3AD.
	Natural variant	117	1	T → S in strain: RAY-3AD.
	Natural variant	144	1	S → STAAAVSQITDGQVQAAKST AAAASQISDGQVQAAKS in strain: RAY-3AD.

Sequences

	Sequence		Length	Mass (Da)
	Q03180-1 [UniParc]. Last modified June 16, 2009. Version 3. Checksum: 7B5670510F8FD1A1	FASTA	325	33,004
	10 20 30 40 50 60 MQYKKPLVVS ALAATSLAAY APKDPWSTLT PSATYKGGIT DYSSSFGIAI EAVATSASSV 70 80 90 100 110 120 ASSKAKRAAS QIGDGQVQAA TTTAAVSKKS TAAAVSQITD GQVQAAKSTA AAVSQITDGQ 130 140 150 160 170 180 VQAAKSTAAA VSQITDGQVQ AAKSTAAAVS QITDGQVQAA KSTAAAASQI SDGQVQATTS 190 200 210 220 230 240 TKAAASQITD GQIQASKTTS GASQVSDGQV QATAEVKDAN DPVDVVSCNN NSTLSMSLSK 250 260 270 280 290 300 GILTDRKGRI GSIVANRQFQ FDGPPPQAGA IYAAGWSITP EGNLALGDQD TFYQCLSGDF 310 320 YNLYDKHIGS QCHEVYLQAI DLIDC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three yeast genes, PIR1, PIR2 and PIR3, containing internal tandem repeats, are related to each other, and PIR1 and PIR2 are required for tolerance to heat shock." Toh-e A., Yasunaga S., Nisogi H., Tanaka K., Oguchi T., Matsui Y. Yeast 9:481-494(1993) [PubMed: 8322511] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RAY-3AD.
[2]	"DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S35-S40(1994) [PubMed: 8091859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Specific labelling of cell wall proteins by biotinylation. Identification of four covalently linked O-mannosylated proteins of Saccharomyces cerevisiae." Mrsa V., Seidl T., Gentzsch M., Tanner W. Yeast 13:1145-1154(1997) [PubMed: 9301021] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-22, CLEAVAGE BY KEX2, GLYCOSYLATION.
[5]	"Partial purification and characterization of early and late endosomes from yeast. Identification of four novel proteins." Singer-Krueger B., Frank R., Crausaz F., Riezman H. J. Biol. Chem. 268:14376-14386(1993) [PubMed: 8314797] [Abstract] Cited for: PROTEIN SEQUENCE OF 68-77. Strain: RH732.
[6]	"Stress proteins on the yeast cell surface determine resistance to osmotin, a plant antifungal protein." Yun D.-J., Zhao Y., Pardo J.M., Narasimhan M.L., Damsz B., Lee H., Abad L.R., D'Urzo M.P., Hasegawa P.M., Bressan R.A. Proc. Natl. Acad. Sci. U.S.A. 94:7082-7087(1997) [PubMed: 9192695] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Genome-wide analysis of gene expression regulated by the yeast cell wall integrity signalling pathway." Jung U.S., Levin D.E. Mol. Microbiol. 34:1049-1057(1999) [PubMed: 10594829] [Abstract] Cited for: INDUCTION.
[8]	"Role of NaOH-extractable cell wall proteins Ccw5p, Ccw6p, Ccw7p and Ccw8p (members of the Pir protein family) in stability of the Saccharomyces cerevisiae cell wall." Mrsa V., Tanner W. Yeast 15:813-820(1999) [PubMed: 10407261] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]	"Overlapping and distinct roles of the duplicated yeast transcription factors Ace2p and Swi5p." Doolin M.-T., Johnson A.L., Johnston L.H., Butler G. Mol. Microbiol. 40:422-432(2001) [PubMed: 11309124] [Abstract] Cited for: INDUCTION.
[10]	"Increased mortality of Saccharomyces cerevisiae cell wall protein mutants." Teparic R., Stuparevic I., Mrsa V. Microbiology 150:3145-3150(2004) [PubMed: 15470095] [Abstract] Cited for: FUNCTION.
[11]	"Characterization of the transcriptional response to cell wall stress in Saccharomyces cerevisiae." Boorsma A., de Nobel H., ter Riet B., Bargmann B., Brul S., Hellingwerf K.J., Klis F.M. Yeast 21:413-427(2004) [PubMed: 15116342] [Abstract] Cited for: INDUCTION.
[12]	"Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages." Yin Q.Y., de Groot P.W.J., Dekker H.L., de Jong L., Klis F.M., de Koster C.G. J. Biol. Chem. 280:20894-20901(2005) [PubMed: 15781460] [Abstract] Cited for: SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[13]	"Intragenic tandem repeats generate functional variability." Verstrepen K.J., Jansen A., Lewitter F., Fink G.R. Nat. Genet. 37:986-990(2005) [PubMed: 16086015] [Abstract] Cited for: REPEATS.
[14]	"Mass spectrometric quantitation of covalently bound cell wall proteins in Saccharomyces cerevisiae." Yin Q.Y., de Groot P.W.J., de Jong L., Klis F.M., de Koster C.G. FEMS Yeast Res. 7:887-896(2007) [PubMed: 17617218] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D13742 Genomic DNA. Translation: BAA02887.1. Z26877 Genomic DNA. Translation: CAA81491.1. Z28163 Genomic DNA. Translation: CAA82005.1.
PIR	S37788.
RefSeq	NP_012758.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4752N.
IntAct	Q03180. 2 interactions.
STRING	Q03180.
2-D gel databases
COMPLUYEAST-2DPAGE	Q03180.
Genome annotation databases
GeneID	853693.
KEGG	sce:YKL163W.
Organism-specific databases
CYGD	YKL163w.
SGD	S000001646. PIR3.
Phylogenomic databases
HOGENOM	HBG626440.
OrthoDB	EOG983FN8.
Gene expression databases
Genevestigator	Q03180.
GermOnline	YKL163W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000420. Yeast_PIR. [Graphical view]
Pfam	PF00399. PIR. 7 hits. [Graphical view]
PROSITE	PS00929. PIR_REPEAT_1. 8 hits. PS50256. PIR_REPEAT_2. 8 hits. [Graphical view]
ProtoNet	Search...
Other Resources
PMAP-CutDB	Q03180.

Entry information

Entry name

PIR3_YEAST

Accession

Primary (citable) accession number: Q03180

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	June 16, 2009
Last modified:	December 15, 2009
This is version 81 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)