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Reviewed, UniProtKB/Swiss-Prot Q03178 (PIR1_YEAST)

Last modified December 15, 2009. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cell wall mannoprotein PIR1
Alternative name(s):
    Protein with internal repeats 1
    Covalently-linked cell wall protein 6
Gene names
Name: PIR1
Synonyms: CCW6
Ordered Locus Names: YKL164C
ORF Names: YKL618
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Component of the outer cell wall layer. Required for stability of the cell wall and for optimal growth. Required for resistance against several antifungal and cell wall-perturbing agents and for tolerance to heat shock. Ref.5 Ref.7 Ref.10

Subcellular location

Secreted › cell wall. Note: Covalently attached to the cell wall. Ref.5 Ref.4 Ref.12

Induction

Positively regulated by signaling through MPK1 in response to cell wall perturbation. Expression is also regulated by the SWI5 transcription factor. Ref.6 Ref.8 Ref.11

Domain

The PIR1/2/3 repeats are required for covalent linkage to the cell wall By similarity. Their number varies among different strains of S.cerevisiae.

Post-translational modification

Covalently linked to beta-1,3-glucan of the inner cell wall layer via an alkali-sensitive ester linkage between the gamma-carboxyl group of glutamic acids, arising from specific glutamines within the PIR1/2/3 repeats, and hydroxyl groups of glucoses of beta-1,3-glucan chains By similarity.

O-glycosylated. Extensively O-mannosylated. Ref.4

Miscellaneous

Present with 1170 molecules/cell in log phase SD medium. Ref.9

Sequence similarities

Belongs to the PIR protein family.

Contains 8 PIR1/2/3 repeats.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Ref.4
Propeptide19 – 6345
PRO_0000033254
Chain64 – 341278Cell wall mannoprotein PIR1
PRO_0000033255

Regions

Repeat64 – 8219PIR1/2/3 1
Repeat83 – 10119PIR1/2/3 2
Repeat102 – 12524PIR1/2/3 3
Repeat126 – 14419PIR1/2/3 4
Repeat145 – 16319PIR1/2/3 5
Repeat164 – 18219PIR1/2/3 6
Repeat183 – 20119PIR1/2/3 7
Repeat202 – 22019PIR1/2/3 8

Sites

Site63 – 642Cleavage; by KEX2
Site741Covalent attachment to cell wall glycan By similarity
Site931Covalent attachment to cell wall glycan By similarity
Site1121Covalent attachment to cell wall glycan By similarity
Site1361Covalent attachment to cell wall glycan By similarity
Site1551Covalent attachment to cell wall glycan By similarity
Site1741Covalent attachment to cell wall glycan By similarity
Site1931Covalent attachment to cell wall glycan By similarity
Site2121Covalent attachment to cell wall glycan By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q03178-1 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 15F0736244769C12

FASTA34134,622
        10         20         30         40         50         60 
MQYKKSLVAS ALVATSLAAY APKDPWSTLT PSATYKGGIT DYSSTFGIAV EPIATTASSK 

        70         80         90        100        110        120 
AKRAAAISQI GDGQIQATTK TTAAAVSQIG DGQIQATTKT KAAAVSQIGD GQIQATTKTT 

       130        140        150        160        170        180 
SAKTTAAAVS QIGDGQIQAT TKTKAAAVSQ IGDGQIQATT KTTAAAVSQI GDGQIQATTK 

       190        200        210        220        230        240 
TTAAAVSQIG DGQIQATTNT TVAPVSQITD GQIQATTLTS ATIIPSPAPA PITNGTDPVT 

       250        260        270        280        290        300 
AETCKSSGTL EMNLKGGILT DGKGRIGSIV ANRQFQFDGP PPQAGAIYAA GWSITPEGNL 

       310        320        330        340 
AIGDQDTFYQ CLSGNFYNLY DEHIGTQCNA VHLQAIDLLN C

« Hide

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References

« Hide 'large scale' references
[1]"Three yeast genes, PIR1, PIR2 and PIR3, containing internal tandem repeats, are related to each other, and PIR1 and PIR2 are required for tolerance to heat shock."
Toh-e A., Yasunaga S., Nisogi H., Tanaka K., Oguchi T., Matsui Y.
Yeast 9:481-494(1993) [PubMed: 8322511] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: RAY-3AD.
[2]"DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae."
Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
Yeast 10:S35-S40(1994) [PubMed: 8091859] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed: 8196765] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Specific labelling of cell wall proteins by biotinylation. Identification of four covalently linked O-mannosylated proteins of Saccharomyces cerevisiae."
Mrsa V., Seidl T., Gentzsch M., Tanner W.
Yeast 13:1145-1154(1997) [PubMed: 9301021] [Abstract]
Cited for: PROTEIN SEQUENCE OF 19-25 AND 64-84, CLEAVAGE BY KEX2, GLYCOSYLATION, SUBCELLULAR LOCATION.
[5]"Stress proteins on the yeast cell surface determine resistance to osmotin, a plant antifungal protein."
Yun D.-J., Zhao Y., Pardo J.M., Narasimhan M.L., Damsz B., Lee H., Abad L.R., D'Urzo M.P., Hasegawa P.M., Bressan R.A.
Proc. Natl. Acad. Sci. U.S.A. 94:7082-7087(1997) [PubMed: 9192695] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"Genome-wide analysis of gene expression regulated by the yeast cell wall integrity signalling pathway."
Jung U.S., Levin D.E.
Mol. Microbiol. 34:1049-1057(1999) [PubMed: 10594829] [Abstract]
Cited for: INDUCTION.
[7]"Role of NaOH-extractable cell wall proteins Ccw5p, Ccw6p, Ccw7p and Ccw8p (members of the Pir protein family) in stability of the Saccharomyces cerevisiae cell wall."
Mrsa V., Tanner W.
Yeast 15:813-820(1999) [PubMed: 10407261] [Abstract]
Cited for: FUNCTION.
[8]"Overlapping and distinct roles of the duplicated yeast transcription factors Ace2p and Swi5p."
Doolin M.-T., Johnson A.L., Johnston L.H., Butler G.
Mol. Microbiol. 40:422-432(2001) [PubMed: 11309124] [Abstract]
Cited for: INDUCTION.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Increased mortality of Saccharomyces cerevisiae cell wall protein mutants."
Teparic R., Stuparevic I., Mrsa V.
Microbiology 150:3145-3150(2004) [PubMed: 15470095] [Abstract]
Cited for: FUNCTION.
[11]"Characterization of the transcriptional response to cell wall stress in Saccharomyces cerevisiae."
Boorsma A., de Nobel H., ter Riet B., Bargmann B., Brul S., Hellingwerf K.J., Klis F.M.
Yeast 21:413-427(2004) [PubMed: 15116342] [Abstract]
Cited for: INDUCTION.
[12]"Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages."
Yin Q.Y., de Groot P.W.J., Dekker H.L., de Jong L., Klis F.M., de Koster C.G.
J. Biol. Chem. 280:20894-20901(2005) [PubMed: 15781460] [Abstract]
Cited for: SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[13]"Intragenic tandem repeats generate functional variability."
Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
Nat. Genet. 37:986-990(2005) [PubMed: 16086015] [Abstract]
Cited for: REPEATS.
+Additional computationally mapped references.

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Cross-references

Sequence databases

D13740 Genomic DNA. Translation: BAA02885.1.
Z26877 Genomic DNA. Translation: CAA81490.1.
Z28164 Genomic DNA. Translation: CAA82006.1.
PIRS33650.
RefSeqNP_012757.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP-4607N.
IntActQ03178. 27 interactions.
STRINGQ03178.

Proteomic databases

PeptideAtlasQ03178.

Genome annotation databases

EnsemblYKL164C; YKL164C; YKL164C; Saccharomyces cerevisiae. [Genome view]
GeneID853692.
KEGGsce:YKL164C.
NMPDRfig|4932.3.peg.3736.

Organism-specific databases

CYGDYKL164c.
SGDS000001647. PIR1.

Phylogenomic databases

HOGENOMHBG626440.
OMAVPAVSQI.
OrthoDBEOG97H76C.

Gene expression databases

ArrayExpressQ03178.
GenevestigatorQ03178.
GermOnlineYKL164C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR000420. Yeast_PIR.
[Graphical view]
PfamPF00399. PIR. 8 hits.
[Graphical view]
PROSITEPS00929. PIR_REPEAT_1. 8 hits.
PS50256. PIR_REPEAT_2. 8 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio974669.

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Entry information

Entry namePIR1_YEAST
AccessionPrimary (citable) accession number: Q03178
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: December 15, 2009
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents