Q03178 (PIR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell wall mannoprotein PIR1

Alternative name(s):
Covalently-linked cell wall protein 6
Protein with internal repeats 1

Gene names

Name:	PIR1
Synonyms:	CCW6
Ordered Locus Names:	YKL164C
ORF Names:	YKL618

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the outer cell wall layer. Required for stability of the cell wall and for optimal growth. Required for resistance against several antifungal and cell wall-perturbing agents and for tolerance to heat shock. Ref.6 Ref.8 Ref.11
Subcellular location	Secreted › cell wall. Note: Covalently attached to the cell wall. Ref.5 Ref.6 Ref.13
Induction	Positively regulated by signaling through MPK1 in response to cell wall perturbation. Expression is also regulated by the SWI5 transcription factor. Ref.7 Ref.9 Ref.12
Domain	The PIR1/2/3 repeats are required for covalent linkage to the cell wall By similarity. Their number varies among different strains of S.cerevisiae.
Post-translational modification	Covalently linked to beta-1,3-glucan of the inner cell wall layer via an alkali-sensitive ester linkage between the gamma-carboxyl group of glutamic acids, arising from specific glutamines within the PIR1/2/3 repeats, and hydroxyl groups of glucoses of beta-1,3-glucan chains By similarity. O-glycosylated. Extensively O-mannosylated. Ref.5
Miscellaneous	Present with 1170 molecules/cell in log phase SD medium. Ref.10
Sequence similarities	Belongs to the PIR protein family. Contains 8 PIR1/2/3 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Repeat Signal
PTM	Cleavage on pair of basic residues Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	fungal-type cell wall organization Inferred from mutant phenotype Ref.8. Source: SGD intracellular protein transport Inferred from physical interaction. Source: SGD
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW fungal-type cell wall Inferred from direct assay Ref.13Ref.6. Source: SGD intracellular Inferred from physical interaction. Source: GOC
Molecular function	structural constituent of cell wall Inferred from physical interaction Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.5
	Propeptide	19 – 63	45			PRO_0000033254
	Chain	64 – 341	278	Cell wall mannoprotein PIR1		PRO_0000033255
Regions
	Repeat	64 – 82	19	PIR1/2/3 1
	Repeat	83 – 101	19	PIR1/2/3 2
	Repeat	102 – 125	24	PIR1/2/3 3
	Repeat	126 – 144	19	PIR1/2/3 4
	Repeat	145 – 163	19	PIR1/2/3 5
	Repeat	164 – 182	19	PIR1/2/3 6
	Repeat	183 – 201	19	PIR1/2/3 7
	Repeat	202 – 220	19	PIR1/2/3 8
Sites
	Site	63 – 64	2	Cleavage; by KEX2
	Site	74	1	Covalent attachment to cell wall glycan By similarity
	Site	93	1	Covalent attachment to cell wall glycan By similarity
	Site	112	1	Covalent attachment to cell wall glycan By similarity
	Site	136	1	Covalent attachment to cell wall glycan By similarity
	Site	155	1	Covalent attachment to cell wall glycan By similarity
	Site	174	1	Covalent attachment to cell wall glycan By similarity
	Site	193	1	Covalent attachment to cell wall glycan By similarity
	Site	212	1	Covalent attachment to cell wall glycan By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q03178 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 15F0736244769C12	FASTA	341	34,622
	10 20 30 40 50 60 MQYKKSLVAS ALVATSLAAY APKDPWSTLT PSATYKGGIT DYSSTFGIAV EPIATTASSK 70 80 90 100 110 120 AKRAAAISQI GDGQIQATTK TTAAAVSQIG DGQIQATTKT KAAAVSQIGD GQIQATTKTT 130 140 150 160 170 180 SAKTTAAAVS QIGDGQIQAT TKTKAAAVSQ IGDGQIQATT KTTAAAVSQI GDGQIQATTK 190 200 210 220 230 240 TTAAAVSQIG DGQIQATTNT TVAPVSQITD GQIQATTLTS ATIIPSPAPA PITNGTDPVT 250 260 270 280 290 300 AETCKSSGTL EMNLKGGILT DGKGRIGSIV ANRQFQFDGP PPQAGAIYAA GWSITPEGNL 310 320 330 340 AIGDQDTFYQ CLSGNFYNLY DEHIGTQCNA VHLQAIDLLN C

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three yeast genes, PIR1, PIR2 and PIR3, containing internal tandem repeats, are related to each other, and PIR1 and PIR2 are required for tolerance to heat shock." Toh-e A., Yasunaga S., Nisogi H., Tanaka K., Oguchi T., Matsui Y. Yeast 9:481-494(1993) [PubMed: 8322511] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RAY-3AD.
[2]	"DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S35-S40(1994) [PubMed: 8091859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Specific labelling of cell wall proteins by biotinylation. Identification of four covalently linked O-mannosylated proteins of Saccharomyces cerevisiae." Mrsa V., Seidl T., Gentzsch M., Tanner W. Yeast 13:1145-1154(1997) [PubMed: 9301021] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-25 AND 64-84, CLEAVAGE BY KEX2, GLYCOSYLATION, SUBCELLULAR LOCATION.
[6]	"Stress proteins on the yeast cell surface determine resistance to osmotin, a plant antifungal protein." Yun D.-J., Zhao Y., Pardo J.M., Narasimhan M.L., Damsz B., Lee H., Abad L.R., D'Urzo M.P., Hasegawa P.M., Bressan R.A. Proc. Natl. Acad. Sci. U.S.A. 94:7082-7087(1997) [PubMed: 9192695] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Genome-wide analysis of gene expression regulated by the yeast cell wall integrity signalling pathway." Jung U.S., Levin D.E. Mol. Microbiol. 34:1049-1057(1999) [PubMed: 10594829] [Abstract] Cited for: INDUCTION.
[8]	"Role of NaOH-extractable cell wall proteins Ccw5p, Ccw6p, Ccw7p and Ccw8p (members of the Pir protein family) in stability of the Saccharomyces cerevisiae cell wall." Mrsa V., Tanner W. Yeast 15:813-820(1999) [PubMed: 10407261] [Abstract] Cited for: FUNCTION.
[9]	"Overlapping and distinct roles of the duplicated yeast transcription factors Ace2p and Swi5p." Doolin M.-T., Johnson A.L., Johnston L.H., Butler G. Mol. Microbiol. 40:422-432(2001) [PubMed: 11309124] [Abstract] Cited for: INDUCTION.
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Increased mortality of Saccharomyces cerevisiae cell wall protein mutants." Teparic R., Stuparevic I., Mrsa V. Microbiology 150:3145-3150(2004) [PubMed: 15470095] [Abstract] Cited for: FUNCTION.
[12]	"Characterization of the transcriptional response to cell wall stress in Saccharomyces cerevisiae." Boorsma A., de Nobel H., ter Riet B., Bargmann B., Brul S., Hellingwerf K.J., Klis F.M. Yeast 21:413-427(2004) [PubMed: 15116342] [Abstract] Cited for: INDUCTION.
[13]	"Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages." Yin Q.Y., de Groot P.W.J., Dekker H.L., de Jong L., Klis F.M., de Koster C.G. J. Biol. Chem. 280:20894-20901(2005) [PubMed: 15781460] [Abstract] Cited for: SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[14]	"Intragenic tandem repeats generate functional variability." Verstrepen K.J., Jansen A., Lewitter F., Fink G.R. Nat. Genet. 37:986-990(2005) [PubMed: 16086015] [Abstract] Cited for: REPEATS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13740 Genomic DNA. Translation: BAA02885.1. Z26877 Genomic DNA. Translation: CAA81490.1. Z28164 Genomic DNA. Translation: CAA82006.1. BK006944 Genomic DNA. Translation: DAA09001.1.
PIR	S33650.
RefSeq	NP_012757.1. NM_001179730.1.
3D structure databases
ProteinModelPortal	Q03178.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4607N.
IntAct	Q03178. 11 interactions.
MINT	MINT-507264.
STRING	Q03178.
Proteomic databases
PeptideAtlas	Q03178.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL164C; YKL164C; YKL164C.
GeneID	853692.
KEGG	sce:YKL164C.
NMPDR	fig|4932.3.peg.3736.
Organism-specific databases
CYGD	YKL164c.
SGD	S000001647. PIR1.
Phylogenomic databases
eggNOG	fuNOG08170.
GeneTree	EFGT00050000002654.
HOGENOM	HBG626440.
OMA	EGNLAIG.
OrthoDB	EOG4GMZ6K.
Gene expression databases
ArrayExpress	Q03178.
Genevestigator	Q03178.
GermOnline	YKL164C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000420. Yeast_PIR. [Graphical view]
Pfam	PF00399. PIR. 8 hits. [Graphical view]
PROSITE	PS00929. PIR_REPEAT_1. 8 hits. PS50256. PIR_REPEAT_2. 8 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	974669.

Entry information

Entry name

PIR1_YEAST

Accession

Primary (citable) accession number: Q03178
Secondary accession number(s): D6VX35

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program