Q03175 (SRT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dehydrodolichyl diphosphate synthase SRT1
Short name=DEDOL-PP synthase
EC=2.5.1.87

Alternative name(s):
Ditrans,polycis-polyprenyl diphosphate synthase ((2E,6E)-farnesyl diphosphate specific)

Gene names

Name:	SRT1
Ordered Locus Names:	YMR101C
ORF Names:	YM6543.08C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cis-prenyl transferase involved in the dolichol synthesis, a family of long-chain polyprenols and which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER). Adds multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP). Ref.4 Ref.5
Catalytic activity	(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = n diphosphate + ditrans,polycis-polyprenyl diphosphate (n = 10-55).
Subcellular location	Lipid droplet Ref.4.
Induction	Expression is induced in the stationary phase. Ref.4
Sequence similarities	Belongs to the UPP synthase family.

Ontologies

Keywords
Cellular component	Lipid droplet
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein glycosylation Inferred from direct assay Ref.4. Source: SGD
Cellular_component	lipid particle Inferred from direct assay Ref.4. Source: SGD
Molecular_function	dehydrodolichyl diphosphate synthase activity Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Dehydrodolichyl diphosphate synthase SRT1		PRO_0000123760

Sequences

	Sequence		Length	Mass (Da)
	Q03175 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 6B9E925663597AF0	FASTA	343	40,200
	10 20 30 40 50 60 MKMPSIIQIQ FVALKRLLVE TKEQMCFAVK SIFQRVFAWV MSLSLFSWFY VNLQNILIKA 70 80 90 100 110 120 LRVGPVPEHV SFIMDGNRRY AKSRRLPVKK GHEAGGLTLL TLLYICKRLG VKCVSAYAFS 130 140 150 160 170 180 IENFNRPKEE VDTLMNLFTV KLDEFAKRAK DYKDPLYGSK IRIVGDQSLL SPEMRKKIKK 190 200 210 220 230 240 VEEITQDGDD FTLFICFPYT SRNDMLHTIR DSVEDHLENK SPRINIRKFT NKMYMGFHSN 250 260 270 280 290 300 KCELLIRTSG HRRLSDYMLW QVHENATIEF SDTLWPNFSF FAMYLMILKW SFFSTIQKYN 310 320 330 340 EKNHSLFEKI HESVPSIFKK KKTAMSLYNF PNPPISVSVT GDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis." Sato M., Sato K., Nishikawa S., Hirata A., Kato J., Nakano A. Mol. Cell. Biol. 19:471-483(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PRELIMINARY CHARACTERIZATION.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles in dolichol synthesis." Sato M., Fujisaki S., Sato K., Nishimura Y., Nakano A. Genes Cells 6:495-506(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION, SUBCELLULAR LOCATION.
[5]	"Precise bacterial polyprenol length control fails in Saccharomyces cerevisiae." Poznanski J., Szkopinska A. Biopolymers 86:155-164(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB013498 Genomic DNA. Translation: BAA36578.1. Z49807 Genomic DNA. Translation: CAA89902.1. BK006946 Genomic DNA. Translation: DAA09998.1.
PIR	S55087.
RefSeq	NP_013819.1. NM_001182601.1.
3D structure databases
ProteinModelPortal	Q03175.
SMR	Q03175. Positions 69-278.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4439N.
IntAct	Q03175. 1 interaction.
MINT	MINT-483050.
STRING	4932.YMR101C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR101C; YMR101C; YMR101C.
GeneID	855127.
KEGG	sce:YMR101C.
Organism-specific databases
CYGD	YMR101c.
SGD	S000004707. SRT1.
Phylogenomic databases
eggNOG	COG0020.
HOGENOM	HOG000006053.
KO	K11778.
OMA	PYTSRND.
OrthoDB	EOG46HKKZ.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14491. YEAST:YMR101C-MONOMER.
Gene expression databases
Genevestigator	Q03175.
GermOnline	YMR101C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.1180.10. 1 hit.
InterPro	IPR001441. UPP_synth-like. IPR018520. UPP_synth-like_CS. [Graphical view]
PANTHER	PTHR10291. PTHR10291. 1 hit.
Pfam	PF01255. Prenyltransf. 1 hit. [Graphical view]
SUPFAM	SSF64005. UPP_synth. 1 hit.
TIGRFAMs	TIGR00055. uppS. 1 hit.
PROSITE	PS01066. UPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978493.

Entry information

Entry name

SRT1_YEAST

Accession

Primary (citable) accession number: Q03175
Secondary accession number(s): D6VZS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 29, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program