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Protein

Dehydrodolichyl diphosphate syntase complex subunit SRT1

Gene

SRT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

With NUS1, forms the dehydrodolichyl diphosphate syntase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Adds multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precusrosor of dolichol which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER).3 Publications

Catalytic activityi

(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = n diphosphate + ditrans,polycis-polyprenyl diphosphate (n = 10-55).1 Publication

Pathwayi

GO - Molecular functioni

  1. dehydrodolichyl diphosphate synthase activity Source: SGD
  2. prenyltransferase activity Source: SGD

GO - Biological processi

  1. protein glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14491.
YEAST:YMR101C-MONOMER.
ReactomeiREACT_188449. Synthesis of Dolichyl-phosphate.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dehydrodolichyl diphosphate syntase complex subunit SRT1Curated (EC:2.5.1.871 Publication)
Alternative name(s):
Ditrans,polycis-polyprenyl diphosphate synthase ((2E,6E)-farnesyl diphosphate specific)Curated
Gene namesi
Name:SRT1Imported
Ordered Locus Names:YMR101CImported
ORF Names:YM6543.08C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR101c.
SGDiS000004707. SRT1.

Subcellular locationi

Lipid droplet 1 Publication

GO - Cellular componenti

  1. lipid particle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Lipid droplet

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343Dehydrodolichyl diphosphate syntase complex subunit SRT1PRO_0000123760Add
BLAST

Expressioni

Inductioni

Expression is induced in the stationary phase.1 Publication

Gene expression databases

GenevestigatoriQ03175.

Interactioni

Subunit structurei

Forms an active dehydrodolichyl diphosphate syntase complex with NUS1.1 Publication

Protein-protein interaction databases

BioGridi35277. 6 interactions.
DIPiDIP-4439N.
IntActiQ03175. 1 interaction.
MINTiMINT-483050.
STRINGi4932.YMR101C.

Structurei

3D structure databases

ProteinModelPortaliQ03175.
SMRiQ03175. Positions 69-278.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UPP synthase family.Curated

Phylogenomic databases

eggNOGiCOG0020.
HOGENOMiHOG000006053.
InParanoidiQ03175.
KOiK11778.
OMAiMLWQVHE.
OrthoDBiEOG73BVQX.

Family and domain databases

Gene3Di3.40.1180.10. 1 hit.
InterProiIPR001441. UPP_synth-like.
IPR018520. UPP_synth-like_CS.
[Graphical view]
PANTHERiPTHR10291. PTHR10291. 1 hit.
PfamiPF01255. Prenyltransf. 1 hit.
[Graphical view]
SUPFAMiSSF64005. SSF64005. 1 hit.
TIGRFAMsiTIGR00055. uppS. 1 hit.
PROSITEiPS01066. UPP_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMPSIIQIQ FVALKRLLVE TKEQMCFAVK SIFQRVFAWV MSLSLFSWFY
60 70 80 90 100
VNLQNILIKA LRVGPVPEHV SFIMDGNRRY AKSRRLPVKK GHEAGGLTLL
110 120 130 140 150
TLLYICKRLG VKCVSAYAFS IENFNRPKEE VDTLMNLFTV KLDEFAKRAK
160 170 180 190 200
DYKDPLYGSK IRIVGDQSLL SPEMRKKIKK VEEITQDGDD FTLFICFPYT
210 220 230 240 250
SRNDMLHTIR DSVEDHLENK SPRINIRKFT NKMYMGFHSN KCELLIRTSG
260 270 280 290 300
HRRLSDYMLW QVHENATIEF SDTLWPNFSF FAMYLMILKW SFFSTIQKYN
310 320 330 340
EKNHSLFEKI HESVPSIFKK KKTAMSLYNF PNPPISVSVT GDE
Length:343
Mass (Da):40,200
Last modified:November 1, 1997 - v1
Checksum:i6B9E925663597AF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013498 Genomic DNA. Translation: BAA36578.1.
Z49807 Genomic DNA. Translation: CAA89902.1.
BK006946 Genomic DNA. Translation: DAA09998.1.
PIRiS55087.
RefSeqiNP_013819.1. NM_001182601.1.

Genome annotation databases

EnsemblFungiiYMR101C; YMR101C; YMR101C.
GeneIDi855127.
KEGGisce:YMR101C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013498 Genomic DNA. Translation: BAA36578.1.
Z49807 Genomic DNA. Translation: CAA89902.1.
BK006946 Genomic DNA. Translation: DAA09998.1.
PIRiS55087.
RefSeqiNP_013819.1. NM_001182601.1.

3D structure databases

ProteinModelPortaliQ03175.
SMRiQ03175. Positions 69-278.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35277. 6 interactions.
DIPiDIP-4439N.
IntActiQ03175. 1 interaction.
MINTiMINT-483050.
STRINGi4932.YMR101C.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR101C; YMR101C; YMR101C.
GeneIDi855127.
KEGGisce:YMR101C.

Organism-specific databases

CYGDiYMR101c.
SGDiS000004707. SRT1.

Phylogenomic databases

eggNOGiCOG0020.
HOGENOMiHOG000006053.
InParanoidiQ03175.
KOiK11778.
OMAiMLWQVHE.
OrthoDBiEOG73BVQX.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciMetaCyc:MONOMER-14491.
YEAST:YMR101C-MONOMER.
ReactomeiREACT_188449. Synthesis of Dolichyl-phosphate.

Miscellaneous databases

NextBioi978493.

Gene expression databases

GenevestigatoriQ03175.

Family and domain databases

Gene3Di3.40.1180.10. 1 hit.
InterProiIPR001441. UPP_synth-like.
IPR018520. UPP_synth-like_CS.
[Graphical view]
PANTHERiPTHR10291. PTHR10291. 1 hit.
PfamiPF01255. Prenyltransf. 1 hit.
[Graphical view]
SUPFAMiSSF64005. SSF64005. 1 hit.
TIGRFAMsiTIGR00055. uppS. 1 hit.
PROSITEiPS01066. UPP_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis."
    Sato M., Sato K., Nishikawa S., Hirata A., Kato J., Nakano A.
    Mol. Cell. Biol. 19:471-483(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PRELIMINARY CHARACTERIZATION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles in dolichol synthesis."
    Sato M., Fujisaki S., Sato K., Nishimura Y., Nakano A.
    Genes Cells 6:495-506(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, SUBCELLULAR LOCATION.
  5. "Precise bacterial polyprenol length control fails in Saccharomyces cerevisiae."
    Poznanski J., Szkopinska A.
    Biopolymers 86:155-164(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Mutation of Nogo-B receptor, a subunit of cis-prenyltransferase, causes a congenital disorder of glycosylation."
    Park E.J., Grabinska K.A., Guan Z., Stranecky V., Hartmannova H., Hodanova K., Baresova V., Sovova J., Jozsef L., Ondruskova N., Hansikova H., Honzik T., Zeman J., Hulkova H., Wen R., Kmoch S., Sessa W.C.
    Cell Metab. 20:448-457(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, FUNCTION, SUBUNIT.

Entry informationi

Entry nameiSRT1_YEAST
AccessioniPrimary (citable) accession number: Q03175
Secondary accession number(s): D6VZS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.