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Q03175

- SRT1_YEAST

UniProt

Q03175 - SRT1_YEAST

Protein

Dehydrodolichyl diphosphate synthase SRT1

Gene

SRT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Cis-prenyl transferase involved in the dolichol synthesis, a family of long-chain polyprenols and which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER). Adds multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP).2 Publications

    Catalytic activityi

    (2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = n diphosphate + ditrans,polycis-polyprenyl diphosphate (n = 10-55).

    GO - Molecular functioni

    1. dehydrodolichyl diphosphate synthase activity Source: SGD
    2. prenyltransferase activity Source: SGD

    GO - Biological processi

    1. protein glycosylation Source: SGD

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14491.
    YEAST:YMR101C-MONOMER.
    ReactomeiREACT_188449. Synthesis of Dolichyl-phosphate.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dehydrodolichyl diphosphate synthase SRT1 (EC:2.5.1.87)
    Short name:
    DEDOL-PP synthase
    Alternative name(s):
    Ditrans,polycis-polyprenyl diphosphate synthase ((2E,6E)-farnesyl diphosphate specific)
    Gene namesi
    Name:SRT1
    Ordered Locus Names:YMR101C
    ORF Names:YM6543.08C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR101c.
    SGDiS000004707. SRT1.

    Subcellular locationi

    Lipid droplet 1 Publication

    GO - Cellular componenti

    1. lipid particle Source: SGD

    Keywords - Cellular componenti

    Lipid droplet

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 343343Dehydrodolichyl diphosphate synthase SRT1PRO_0000123760Add
    BLAST

    Expressioni

    Inductioni

    Expression is induced in the stationary phase.1 Publication

    Gene expression databases

    GenevestigatoriQ03175.

    Interactioni

    Protein-protein interaction databases

    BioGridi35277. 6 interactions.
    DIPiDIP-4439N.
    IntActiQ03175. 1 interaction.
    MINTiMINT-483050.
    STRINGi4932.YMR101C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ03175.
    SMRiQ03175. Positions 69-278.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the UPP synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG0020.
    HOGENOMiHOG000006053.
    KOiK11778.
    OMAiMLWQVHE.
    OrthoDBiEOG73BVQX.

    Family and domain databases

    Gene3Di3.40.1180.10. 1 hit.
    InterProiIPR001441. UPP_synth-like.
    IPR018520. UPP_synth-like_CS.
    [Graphical view]
    PANTHERiPTHR10291. PTHR10291. 1 hit.
    PfamiPF01255. Prenyltransf. 1 hit.
    [Graphical view]
    SUPFAMiSSF64005. SSF64005. 1 hit.
    TIGRFAMsiTIGR00055. uppS. 1 hit.
    PROSITEiPS01066. UPP_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q03175-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKMPSIIQIQ FVALKRLLVE TKEQMCFAVK SIFQRVFAWV MSLSLFSWFY    50
    VNLQNILIKA LRVGPVPEHV SFIMDGNRRY AKSRRLPVKK GHEAGGLTLL 100
    TLLYICKRLG VKCVSAYAFS IENFNRPKEE VDTLMNLFTV KLDEFAKRAK 150
    DYKDPLYGSK IRIVGDQSLL SPEMRKKIKK VEEITQDGDD FTLFICFPYT 200
    SRNDMLHTIR DSVEDHLENK SPRINIRKFT NKMYMGFHSN KCELLIRTSG 250
    HRRLSDYMLW QVHENATIEF SDTLWPNFSF FAMYLMILKW SFFSTIQKYN 300
    EKNHSLFEKI HESVPSIFKK KKTAMSLYNF PNPPISVSVT GDE 343
    Length:343
    Mass (Da):40,200
    Last modified:November 1, 1997 - v1
    Checksum:i6B9E925663597AF0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB013498 Genomic DNA. Translation: BAA36578.1.
    Z49807 Genomic DNA. Translation: CAA89902.1.
    BK006946 Genomic DNA. Translation: DAA09998.1.
    PIRiS55087.
    RefSeqiNP_013819.1. NM_001182601.1.

    Genome annotation databases

    EnsemblFungiiYMR101C; YMR101C; YMR101C.
    GeneIDi855127.
    KEGGisce:YMR101C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB013498 Genomic DNA. Translation: BAA36578.1 .
    Z49807 Genomic DNA. Translation: CAA89902.1 .
    BK006946 Genomic DNA. Translation: DAA09998.1 .
    PIRi S55087.
    RefSeqi NP_013819.1. NM_001182601.1.

    3D structure databases

    ProteinModelPortali Q03175.
    SMRi Q03175. Positions 69-278.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35277. 6 interactions.
    DIPi DIP-4439N.
    IntActi Q03175. 1 interaction.
    MINTi MINT-483050.
    STRINGi 4932.YMR101C.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR101C ; YMR101C ; YMR101C .
    GeneIDi 855127.
    KEGGi sce:YMR101C.

    Organism-specific databases

    CYGDi YMR101c.
    SGDi S000004707. SRT1.

    Phylogenomic databases

    eggNOGi COG0020.
    HOGENOMi HOG000006053.
    KOi K11778.
    OMAi MLWQVHE.
    OrthoDBi EOG73BVQX.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-14491.
    YEAST:YMR101C-MONOMER.
    Reactomei REACT_188449. Synthesis of Dolichyl-phosphate.

    Miscellaneous databases

    NextBioi 978493.

    Gene expression databases

    Genevestigatori Q03175.

    Family and domain databases

    Gene3Di 3.40.1180.10. 1 hit.
    InterProi IPR001441. UPP_synth-like.
    IPR018520. UPP_synth-like_CS.
    [Graphical view ]
    PANTHERi PTHR10291. PTHR10291. 1 hit.
    Pfami PF01255. Prenyltransf. 1 hit.
    [Graphical view ]
    SUPFAMi SSF64005. SSF64005. 1 hit.
    TIGRFAMsi TIGR00055. uppS. 1 hit.
    PROSITEi PS01066. UPP_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis."
      Sato M., Sato K., Nishikawa S., Hirata A., Kato J., Nakano A.
      Mol. Cell. Biol. 19:471-483(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PRELIMINARY CHARACTERIZATION.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles in dolichol synthesis."
      Sato M., Fujisaki S., Sato K., Nishimura Y., Nakano A.
      Genes Cells 6:495-506(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION, SUBCELLULAR LOCATION.
    5. "Precise bacterial polyprenol length control fails in Saccharomyces cerevisiae."
      Poznanski J., Szkopinska A.
      Biopolymers 86:155-164(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiSRT1_YEAST
    AccessioniPrimary (citable) accession number: Q03175
    Secondary accession number(s): D6VZS4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3