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Protein

Beta-lactamase PSE-1

Gene

pse1

Organism
Pseudomonas aeruginosa
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes penicillin, ampicillin and carbenicillin but not other antibiotics including oxacillin, methicillin and cloxacillin.1 Publication

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by p-chloromercuribenzoate but not by cloxacillin.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Acyl-ester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase PSE-1 (EC:3.5.2.6)
Alternative name(s):
Beta-lactamase CARB-2
Carbenicillinase 2
Gene namesi
Name:pse11 Publication
Synonyms:carB2
Encoded oniPlasmid RPL112 Publications
OrganismiPseudomonas aeruginosa
Taxonomic identifieri287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Chaini18 – 288271Beta-lactamase PSE-1PRO_0000017046Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi72 ↔ 118By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

Constitutive (at protein level).1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ03170.
SMRiQ03170. Positions 20-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni229 – 2313Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q03170-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFLLAFSLL IPSVVFASSS KFQQVEQDVK AIEVSLSARI GVSVLDTQNG
60 70 80 90 100
EYWDYNGNQR FPLTSTFKTI ACAKLLYDAE QGKVNPNSTV EIKKADLVTY
110 120 130 140 150
SPVIEKQVGQ AITLDDACFA TMTTSDNTAA NIILSAVGGP KGVTDFLRQI
160 170 180 190 200
GDKETRLDRI EPDLNEGKLG DLRDTTTPKA IASTLNKFLF GSALSEMNQK
210 220 230 240 250
KLESWMVNNQ VTGNLLRSVL PAGWNIADRS GAGGFGARSI TAVVWSEHQA
260 270 280
PIIVSIYLAQ TQASMAERND AIVKIGHSIF DVYTSQSR
Length:288
Mass (Da):31,348
Last modified:October 1, 1994 - v1
Checksum:iBA618F058720F4C8
GO

Sequence cautioni

The sequence AAA25740.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z18955 Genomic DNA. Translation: CAA79480.1.
M69058 Genomic DNA. Translation: AAA25741.1.
M69058 Genomic DNA. Translation: AAA25740.1. Different initiation.
RefSeqiWP_001931474.1. NG_036333.1.
YP_009080944.1. NG_036333.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z18955 Genomic DNA. Translation: CAA79480.1.
M69058 Genomic DNA. Translation: AAA25741.1.
M69058 Genomic DNA. Translation: AAA25740.1. Different initiation.
RefSeqiWP_001931474.1. NG_036333.1.
YP_009080944.1. NG_036333.1.

3D structure databases

ProteinModelPortaliQ03170.
SMRiQ03170. Positions 20-285.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiQ03170.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Transposon: Tn1403.
    Plasmid: RPL11
  2. "Properties of the beta-lactamase specified by the Pseudomonas plasmid RPL11."
    Matthew M., Sykes R.B.
    J. Bacteriol. 132:341-345(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBSTRATE SPECIFICITY, ENZYME REGULATION, SUBUNIT, INDUCTION.
    Plasmid: RPL11

Entry informationi

Entry nameiBLP1_PSEAI
AccessioniPrimary (citable) accession number: Q03170
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 24, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.