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Q03170 (BLP1_PSEAI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase PSE-1

EC=3.5.2.6
Alternative name(s):
Beta-lactamase CARB-2
Carbenicillinase 2
Gene names
Name:pse1
Synonyms:carB2
Encoded onPlasmid
OrganismPseudomonas aeruginosa
Taxonomic identifier287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Hydrolyzes both carbenicillin and oxacillin.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Sequence caution

The sequence AAA25740.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   PTMDisulfide bond
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 288271Beta-lactamase PSE-1
PRO_0000017046

Regions

Region229 – 2313Substrate binding By similarity

Sites

Active site651Acyl-ester intermediate By similarity

Amino acid modifications

Disulfide bond72 ↔ 118 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q03170 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: BA618F058720F4C8

FASTA28831,348
        10         20         30         40         50         60 
MKFLLAFSLL IPSVVFASSS KFQQVEQDVK AIEVSLSARI GVSVLDTQNG EYWDYNGNQR 

        70         80         90        100        110        120 
FPLTSTFKTI ACAKLLYDAE QGKVNPNSTV EIKKADLVTY SPVIEKQVGQ AITLDDACFA 

       130        140        150        160        170        180 
TMTTSDNTAA NIILSAVGGP KGVTDFLRQI GDKETRLDRI EPDLNEGKLG DLRDTTTPKA 

       190        200        210        220        230        240 
IASTLNKFLF GSALSEMNQK KLESWMVNNQ VTGNLLRSVL PAGWNIADRS GAGGFGARSI 

       250        260        270        280 
TAVVWSEHQA PIIVSIYLAQ TQASMAERND AIVKIGHSIF DVYTSQSR 

« Hide

References

[1]"Sequence of the PSE-1 beta-lactamase gene."
Huovinen P., Jacoby G.A.
Antimicrob. Agents Chemother. 35:2428-2430(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z18955 Genomic DNA. Translation: CAA79480.1.
M69058 Genomic DNA. Translation: AAA25741.1.
M69058 Genomic DNA. Translation: AAA25740.1. Different initiation.

3D structure databases

ProteinModelPortalQ03170.
SMRQ03170. Positions 20-285.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLP1_PSEAI
AccessionPrimary (citable) accession number: Q03170
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: July 9, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families