Q03170 (BLP1_PSEAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase PSE-1
EC=3.5.2.6

Alternative name(s):
Beta-lactamase CARB-2
Carbenicillinase 2

Gene names

Name:	pse1
Synonyms:	carB2

Encoded on

Plasmid

Organism

Pseudomonas aeruginosa

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolyzes both carbenicillin and oxacillin.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.
Sequence caution	The sequence AAA25740.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 288	271	Beta-lactamase PSE-1		PRO_0000017046
Regions
	Region	229 – 231	3	Substrate binding By similarity
Sites
	Active site	65	1	Acyl-ester intermediate By similarity
Amino acid modifications
	Disulfide bond	72 ↔ 118		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q03170 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: BA618F058720F4C8	FASTA	288	31,348
	10 20 30 40 50 60 MKFLLAFSLL IPSVVFASSS KFQQVEQDVK AIEVSLSARI GVSVLDTQNG EYWDYNGNQR 70 80 90 100 110 120 FPLTSTFKTI ACAKLLYDAE QGKVNPNSTV EIKKADLVTY SPVIEKQVGQ AITLDDACFA 130 140 150 160 170 180 TMTTSDNTAA NIILSAVGGP KGVTDFLRQI GDKETRLDRI EPDLNEGKLG DLRDTTTPKA 190 200 210 220 230 240 IASTLNKFLF GSALSEMNQK KLESWMVNNQ VTGNLLRSVL PAGWNIADRS GAGGFGARSI 250 260 270 280 TAVVWSEHQA PIIVSIYLAQ TQASMAERND AIVKIGHSIF DVYTSQSR

References

[1]	"Sequence of the PSE-1 beta-lactamase gene." Huovinen P., Jacoby G.A. Antimicrob. Agents Chemother. 35:2428-2430(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z18955 Genomic DNA. Translation: CAA79480.1. M69058 Genomic DNA. Translation: AAA25741.1. M69058 Genomic DNA. Translation: AAA25740.1. Different initiation.
3D structure databases
ProteinModelPortal	Q03170.
SMR	Q03170. Positions 20-285.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLP1_PSEAI

Accession

Primary (citable) accession number: Q03170

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program