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Reviewed, UniProtKB/Swiss-Prot Q03168 (ASPP_AEDAE)

Last modified December 15, 2009. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Lysosomal aspartic protease
    EC=3.4.23.-
Gene names
ORF Names: AAEL006169
OrganismAedes aegypti (Yellowfever mosquito) (Culex aegypti) [Complete proteome]
Taxonomic identifier7159 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Culicinae › Culicini › Aedes › Stegomyia

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Protein attributes

Sequence length387 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

May degrade organelles involved in the biosynthesis and secretion of vitellogenin. Ref.3

Subunit structure

Homodimer.

Subcellular location

Lysosome Ref.3.

Sequence similarities

Belongs to the peptidase A1 family.

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Ontologies

Keywords
   Cellular componentLysosome
   DomainSignal
   Molecular functionAspartyl protease
Hydrolase
Protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentlysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionaspartic-type endopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818
Propeptide19 – 5335Removed in mature form
PRO_0000025933
Chain54 – 387334Lysosomal aspartic protease
PRO_0000025934

Sites

Active site861 By similarity
Active site2721 By similarity

Amino acid modifications

Glycosylation1231N-linked (GlcNAc...) Potential
Disulfide bond99 ↔ 106 By similarity
Disulfide bond263 ↔ 267 By similarity
Disulfide bond306 ↔ 343 By similarity

Experimental info

Sequence conflict161A → S in AAA29350. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q03168-1 [UniParc].

Last modified July 22, 2008. Version 2.
Checksum: B9AD73E444167EEB

FASTA38741,790
        10         20         30         40         50         60 
MLIKSIIALV CLAVLAQADF VRVQLHKTES ARQHFRNVDT EIKQLRLKYN AVSGPVPEPL 

        70         80         90        100        110        120 
SNYLDAQYYG AITIGTPPQS FKVVFDTGSS NLWVPSKECS FTNIACLMHN KYNAKKSSTF 

       130        140        150        160        170        180 
EKNGTAFHIQ YGSGSLSGYL STDTVGLGGV SVTKQTFAEA INEPGLVFVA AKFDGILGLG 

       190        200        210        220        230        240 
YSSISVDGVV PVFYNMFNQG LIDAPVFSFY LNRDPSAAEG GEIIFGGSDS NKYTGDFTYL 

       250        260        270        280        290        300 
SVDRKAYWQF KMDSVKVGDT EFCNNGCEAI ADTGTSLIAG PVSEVTAINK AIGGTPIMNG 

       310        320        330        340        350        360 
EYMVDCSLIP KLPKISFVLG GKSFDLEGAD YVLRVAQMGK TICLSGFMGI DIPPPNGPLW 

       370        380 
ILGDVFIGKY YTEFDMGNDR VGFATAV

« Hide

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References

« Hide 'large scale' references
[1]"Cloning of cDNA for mosquito lysosomal aspartic protease. Sequence analysis of an insect lysosomal enzyme similar to cathepsins D and E."
Cho W.L., Raikhel A.S.
J. Biol. Chem. 267:21823-21829(1992) [PubMed: 1400492] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 54-71.
[2]"Genome sequence of Aedes aegypti, a major arbovirus vector."
Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P. expand/collapse author list , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.
Science 316:1718-1723(2007) [PubMed: 17510324] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Liverpool.
[3]"Purification and characterization of a lysosomal aspartic protease with cathepsin D activity from the mosquito."
Cho W.-L., Dhadialla T.S., Raikhel A.S.
Insect Biochem. 21:165-176(1991)
Cited for: FUNCTION, SUBCELLULAR LOCATION.

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Cross-references

Sequence databases

M95187 mRNA. Translation: AAA29350.1.
CH477377 Genomic DNA. Translation: EAT42285.1.
PIRA45117.
RefSeqXP_001657556.1.
UniGeneAae.18587

3D structure databases

ModBaseSearch...

Protein family/group databases

MEROPSA01.009.

2-D gel databases

Siena-2DPAGEQ03168.

Genome annotation databases

GeneID5567565.
KEGGaag:AaeL_AAEL006169.
VectorBaseAAEL006169. Aedes aegypti.

Phylogenomic databases

InParanoidQ03168.
OMASGFMGID.
OrthoDBEOG99W2P3.

Family and domain databases

InterProIPR001461. Peptidase_A1.
IPR001969. Peptidase_aspartic_AS.
IPR009007. Peptidase_aspartic_catalytic.
IPR012848. Propep_A1.
[Graphical view]
Gene3DG3DSA:2.40.70.10. Pept_Aspartc_cat. 2 hits.
PANTHERPTHR13683. Peptidase_A1. 1 hit.
PfamPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSPR00792. PEPSIN.
PROSITEPS00141. ASP_PROTEASE. 2 hits.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameASPP_AEDAE
AccessionPrimary (citable) accession number: Q03168
Secondary accession number(s): Q177E0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 22, 2008
Last modified: December 15, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents