Q03163 (HMDH_CATRO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxy-3-methylglutaryl-coenzyme A reductase
Short name=HMG-CoA reductase
EC=1.1.1.34

Gene names

Name:

HMGR

Organism

Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)

Taxonomic identifier

4058 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Apocynaceae › Rauvolfioideae › Vinceae › Catharanthus

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 601	601	3-hydroxy-3-methylglutaryl-coenzyme A reductase		PRO_0000114437
Regions
	Transmembrane	36 – 58	23	Helical; Potential
	Transmembrane	86 – 106	21	Helical; Potential
	Region	107 – 179	73	Linker By similarity
	Region	180 – 601	422	Catalytic By similarity
Sites
	Active site	273	1	Charge relay system By similarity
	Active site	405	1	Charge relay system By similarity
	Active site	481	1	Charge relay system By similarity
	Active site	579	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	337	1	N-linked (GlcNAc...) Potential
	Glycosylation	583	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q03163 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: D49D00D4F5DB9113	FASTA	601	64,107
	10 20 30 40 50 60 MDSRRRSPTV TAKAAAGELP LAPHEGQNQQ PSIPRSSDVL PLPLYLANGV FFTLFFSVMY 70 80 90 100 110 120 FLLTRWREKI RNATPLHVVT LSELAALASL IASVIYLVSF FGLDFVQSLI YKPNNEGWEI 130 140 150 160 170 180 EEEILMVEDS RNGTNCTTLG CAVPPPSVPK IAPVVPQQPS KMVIIEKPAP LITPQNSEED 190 200 210 220 230 240 EDIIKAVVAG KIPSYSLESK LGDCKRAAGI RREALQRITG KSLEGLPLEG FDYASILGQC 250 260 270 280 290 300 CEMPVGYVQL PVGIAGPLLL DGREYMLPMA TTEGCLVAST NRGCKAILAS GGANSVLLRD 310 320 330 340 350 360 GMTRAPVVRF GTAKRAAELK FYMEDTQNFE TISVVFNKSS RFAKLQSVQC AIAGKNLYIR 370 380 390 400 410 420 FSCSTGDAMG MNMVSKGVQN VLEFLQTDYP DMDVLGISGN FCADKKPAAV NWIEGRGKSV 430 440 450 460 470 480 VCEAIIKEEI VKTVLKTEVA ALIELNMVKN LAGSAIAGAL GGFNAHASNI VSAIFIATGQ 490 500 510 520 530 540 DPAQNVESSQ CITMMEAVND GKDLHISVTM PSIEVGTVGG GTQLASQSAC LNLLGVKGAS 550 560 570 580 590 600 KDSPGANSRL LATIVAGSVL AGELSLMSAI SAGQLVRSHM KYNRSSKDIT NIASSQLESD S

References

[1]	"Nucleotide sequence of a cDNA encoding 3-hydroxy-3-methylglutaryl-CoA reductase from Catharanthus roseus." Maldenado-Mendoza I.E., Burnett R.J., Nessler C.L. Plant Physiol. 100:1613-1614(1992) [PubMed: 16653173] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Little Delicata. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96068 mRNA. Translation: AAA33108.1.
PIR	T09967.
3D structure databases
ProteinModelPortal	Q03163.
SMR	Q03163. Positions 176-576.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase. IPR023074. HMG_CoA_Rdtase_cat. IPR023076. HMG_CoA_Rdtase_CS. IPR004554. HMG_CoA_Rdtase_eu_arc. IPR023282. HMG_CoA_Rdtase_N. IPR009023. HMG_CoA_Rdtase_NAD(P)-bd. IPR009029. HMG_CoA_Rdtase_sub-bd. [Graphical view]
Gene3D	G3DSA:3.30.70.420. G3DSA:3.30.70.420. 1 hit. G3DSA:3.90.770.10. HMG-CoA_red. 2 hits. G3DSA:1.10.3270.10. HMG_CoA_Rdtase_N. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
SUPFAM	SSF55035. HMG_CoA_NAD_bind. 1 hit. SSF56542. HMG_CoA_sub_bind. 1 hit.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH_CATRO

Accession

Primary (citable) accession number: Q03163

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 28, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program