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Protein

Growth factor receptor-bound protein 7

Gene

Grb7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration (By similarity). Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • negative regulation of translation Source: UniProtKB
  • positive regulation of cell migration Source: UniProtKB
  • signal transduction Source: MGI
  • stress granule assembly Source: UniProtKB

Keywordsi

Molecular functionRepressor, RNA-binding
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-1306955 GRB7 events in ERBB2 signaling
R-MMU-1433557 Signaling by SCF-KIT
R-MMU-186763 Downstream signal transduction
R-MMU-210993 Tie2 Signaling
R-MMU-8853659 RET signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Growth factor receptor-bound protein 7
Alternative name(s):
Epidermal growth factor receptor GRB-7
GRB7 adapter protein
Gene namesi
Name:Grb7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:102683 Grb7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi262Y → F: Abolishes interaction with FHL2. 1
Mutagenesisi263Y → F: Abolishes interaction with FHL2. 1
Mutagenesisi287Y → F: Abolishes interaction with FHL2. 1
Mutagenesisi483Y → F: Strongly reduced tyrosine phosphorylation. Abolishes interaction with FHL2. 1 Publication1
Mutagenesisi495Y → F: Strongly reduced tyrosine phosphorylation. Abolishes interaction with FHL2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001503451 – 535Growth factor receptor-bound protein 7Add BLAST535

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei187Phosphotyrosine; by FAK1By similarity1
Modified residuei341Phosphotyrosine; by FAK1By similarity1
Modified residuei364PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on serine and threonine residues in response to activation of receptor kinases. Phosphorylated on tyrosine residues by TEK/TIE2. Phosphorylated on tyrosine residues by PTK2/FAK1, and possibly also other kinases. Phosphorylation is enhanced by activation of receptor kinases. Tyrosine phosphorylation is essential for activation of down-stream protein kinases (By similarity). Phosphorylated on tyrosine residues in response to NTN1 signaling. Phosphorylation promotes stress granule disassembly during recovery after cellular stress.By similarity3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ03160
PaxDbiQ03160
PeptideAtlasiQ03160
PRIDEiQ03160

PTM databases

iPTMnetiQ03160
PhosphoSitePlusiQ03160

Expressioni

Gene expression databases

BgeeiENSMUSG00000019312
CleanExiMM_GRB7
ExpressionAtlasiQ03160 baseline and differential
GenevisibleiQ03160 MM

Interactioni

Subunit structurei

Homodimer. Interacts (via SH2 domain) with EGFR, ERBB2, ERBB3 (when phosphorylated), ERBB4 (when phosphorylated), EPHB1, INSR, FGFR1, PDGFRA (tyrosine phosphorylated) and PDGFRB (tyrosine phosphorylated). Interacts with SHC1. Interacts with RND1. Interacts (when tyrosine phosphorylated) with FHL2 and HAX1 (By similarity). Interacts (via SH2 domain) with RET and PTK2/FAK1. Interacts (when not phosphorylated) with ELAVL1. In stressed cells, but not in normal cells, part of a complex that contains at least GRB7, PTK2/FAK1, STAU1, ELAVL1 and TIA1. Interacts (via SH2 domain) with KIT (phosphorylated). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated).By similarity6 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: MGI
  • protein kinase binding Source: MGI
  • SH3/SH2 adaptor activity Source: MGI

Protein-protein interaction databases

BioGridi200047, 1 interactor
DIPiDIP-43965N
IntActiQ03160, 7 interactors
MINTiQ03160
STRINGi10090.ENSMUSP00000019456

Structurei

3D structure databases

ProteinModelPortaliQ03160
SMRiQ03160
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini99 – 185Ras-associatingPROSITE-ProRule annotationAdd BLAST87
Domaini228 – 341PHPROSITE-ProRule annotationAdd BLAST114
Domaini434 – 530SH2PROSITE-ProRule annotationAdd BLAST97

Domaini

The PH domain mediates interaction with membranes containing phosphoinositides.By similarity

Sequence similaritiesi

Belongs to the GRB7/10/14 family.Curated

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiKOG3751 Eukaryota
ENOG410XXC8 LUCA
GeneTreeiENSGT00550000074537
HOGENOMiHOG000231904
HOVERGENiHBG000468
InParanoidiQ03160
OMAiLSDENWG
OrthoDBiEOG091G135G
PhylomeDBiQ03160
TreeFamiTF317511

Family and domain databases

Gene3Di2.30.29.30, 1 hit
3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR015042 BPS-dom
IPR035032 Grb7
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000159 RA_dom
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR11243:SF25 PTHR11243:SF25, 1 hit
PfamiView protein in Pfam
PF08947 BPS, 1 hit
PF00169 PH, 1 hit
PF00788 RA, 1 hit
PF00017 SH2, 1 hit
PRINTSiPR00401 SH2DOMAIN
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00314 RA, 1 hit
SM00252 SH2, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
PS50200 RA, 1 hit
PS50001 SH2, 1 hit

Sequencei

Sequence statusi: Complete.

Q03160-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELDLSPTHL SSSPEDVCPT PATPPETPPP PDNPPPGDVK RSQPLPIPSS
60 70 80 90 100
RKLREEEFQA TSLPSIPNPF PELCSPPSQK PILGGSSGAR GLLPRDSSRL
110 120 130 140 150
CVVKVYSEDG ACRSVEVAAG ATARHVCEML VQRAHALSDE SWGLVESHPY
160 170 180 190 200
LALERGLEDH EFVVEVQEAW PVGGDSRFIF RKNFAKYELF KSPPHTLFPE
210 220 230 240 250
KMVSSCLDAQ TGISHEDLIQ NFLNAGSFPE IQGFLQLRGS GRGSGRKLWK
260 270 280 290 300
RFFCFLRRSG LYYSTKGTSK DPRHLQYVAD VNESNVYVVT QGRKLYGMPT
310 320 330 340 350
DFGFCVKPNK LRNGHKGLHI FCSEDEQSRT CWLAAFRLFK YGVQLYKNYQ
360 370 380 390 400
QAQSRHLRLS YLGSPPLRSV SDNTLVAMDF SGHAGRVIDN PREALSAAME
410 420 430 440 450
EAQAWRKKTN HRLSLPTTCS GSSLSAAIHR TQPWFHGRIS REESQRLIGQ
460 470 480 490 500
QGLVDGVFLV RESQRNPQGF VLSLCHLQKV KHYLILPSED EGCLYFSMDE
510 520 530
GQTRFTDLLQ LVEFHQLNRG ILPCLLRHCC ARVAL
Length:535
Mass (Da):59,959
Last modified:November 1, 1996 - v1
Checksum:iCD8C307864703645
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94450 mRNA Translation: AAA37733.1
AK145996 mRNA Translation: BAE26817.1
AK168438 mRNA Translation: BAE40343.1
AL591390 Genomic DNA Translation: CAM22007.1
CH466556 Genomic DNA Translation: EDL16144.1
BC003295 mRNA Translation: AAH03295.1
CCDSiCCDS25351.1
PIRiC46243
RefSeqiNP_034476.1, NM_010346.2
XP_006532296.1, XM_006532233.1
UniGeneiMm.276702

Genome annotation databases

EnsembliENSMUST00000019456; ENSMUSP00000019456; ENSMUSG00000019312
GeneIDi14786
KEGGimmu:14786
UCSCiuc007lgk.1 mouse

Similar proteinsi

Entry informationi

Entry nameiGRB7_MOUSE
AccessioniPrimary (citable) accession number: Q03160
Secondary accession number(s): Q3TH55
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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