Reviewed, UniProtKB/Swiss-Prot Q03146 (DDR1_MOUSE)

Names and origin

Protein names

Recommended name:
    Epithelial discoidin domain-containing receptor 1
      Short name=Epithelial discoidin domain receptor 1
    EC=2.7.10.1
Alternative name(s):
    Tyrosine kinase DDR
    Discoidin receptor tyrosine kinase
    Tyrosine-protein kinase CAK
    Cell adhesion kinase
    Protein-tyrosine kinase MPK-6
    CD167 antigen-like family member A
    CD_antigen=CD167a

Gene names

Name:	Ddr1
Synonyms:	Cak, Eddr1, Mpk6

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	911 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May be involved in cell-cell interactions and recognition By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Isoform 1 is predominant and is expressed in developing embryo and adult brain. Isoform 2 is expressed in various epithelial cells.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 1 F5/8 type C domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	branching involved in mammary gland duct morphogenesis Inferred from mutant phenotype. Source: MGI cell adhesion Inferred from electronic annotation. Source: InterPro ear development Inferred from mutant phenotype. Source: MGI embryo implantation Inferred from mutant phenotype. Source: MGI mammary gland alveolus development Inferred from mutant phenotype. Source: MGI negative regulation of cell proliferation Inferred from mutant phenotype. Source: MGI peptidyl-tyrosine phosphorylation Inferred from direct assay. Source: MGI regulation of cell growth Inferred from mutant phenotype. Source: MGI regulation of cell-matrix adhesion Inferred from mutant phenotype. Source: MGI response to protein stimulus Inferred from mutant phenotype. Source: MGI transmembrane receptor protein tyrosine kinase signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro transmembrane receptor protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q03146-1)

Also known as: CAK I;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q03146-2)

Also known as: CAK II;

The sequence of this isoform differs from the canonical sequence as follows:
     503-539: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 911	892	Epithelial discoidin domain-containing receptor 1		PRO_0000016743
Regions
	Topological domain	20 – 414	395	Extracellular Potential
	Transmembrane	415 – 441	27	Potential
	Topological domain	442 – 911	470	Cytoplasmic Potential
	Domain	32 – 186	155	F5/8 type C
	Domain	608 – 903	296	Protein kinase
	Nucleotide binding	614 – 622	9	ATP By similarity
	Compositional bias	379 – 413	35	Gly/Pro-rich
	Compositional bias	474 – 599	126	Gly/Pro-rich
Sites
	Active site	764	1	Proton acceptor By similarity
	Binding site	653	1	ATP By similarity
Amino acid modifications
	Modified residue	511	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	790	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	794	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	795	1	Phosphotyrosine; by autocatalysis By similarity
	Glycosylation	213	1	N-linked (GlcNAc...) Potential
	Glycosylation	262	1	N-linked (GlcNAc...) Potential
	Glycosylation	372	1	N-linked (GlcNAc...) Potential
	Glycosylation	392	1	N-linked (GlcNAc...) Potential
	Disulfide bond	32 ↔ 186		By similarity
Natural variations
	Alternative sequence	503 – 539	37	Missing in isoform 2.		VSP_002954

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (CAK I) [UniParc]. Last modified November 1, 1997. Version 2. Checksum: DBB7FE03DDD79510	FASTA	911	101,161
	10 20 30 40 50 60 MGTGTLSSLL LLLLLVTIGD ADMKGHFDPA KCRYALGMQD RTIPDSDISV SSSWSDSTAA 70 80 90 100 110 120 RHSRLESSDG DGAWCPAGPV FPKEEEYLQV DLRRLHLVAL VGTQGRHAGG LGKEFSRSYR 130 140 150 160 170 180 LRYSRDGRRW MDWKDRWGQE VISGNEDPGG VVLKDLGPPM VARLVRFYPR ADRVMSVCLR 190 200 210 220 230 240 VELYGCLWRD GLLSYTAPVG QTMQLSEVMV HLNDSTYDGY TAGGLQYGGL GQLADGVVGL 250 260 270 280 290 300 DDFRQSQELR VWPGYDYVGW SNQSFPTGYV EMEFEFDRLR TFQTMQVHCN NMHTLGARLP 310 320 330 340 350 360 GGVECRFKRG PAMAWEGEPV RHALGGSLGD PRARAISVPL GGHVGRFLQC RFLFAGPWLL 370 380 390 400 410 420 FSEISFISDV VNDSSDTFPP APWWPPGPPP TNFSSLELEP RGQQPVAKAE GSPTAILIGC 430 440 450 460 470 480 LVAIILLLLL IIALMLWRLH WRRLLSKAER RVLEEELTVH LSVPGDTILI NNRPGPREPP 490 500 510 520 530 540 PYQEPRPRGT PPHSAPCVPN GSALLLSNPA YRLLLATYAR PPRGPGPPTP AWAKPTNTQA 550 560 570 580 590 600 CSGDYMEPEK PGAPLLPPPP QNSVPHYAEA DIVTLQGVTG GNTYAVPALP PGAVGDGPPR 610 620 630 640 650 660 VDFPRSRLRF KEKLGEGQFG EVHLCEVEDP QDLVSSDFPI SVHKGHPLLV AVKILRPDAT 670 680 690 700 710 720 KNARNDFLKE VKIMSRLKDP NIIRLLGVCV QDDPLCMITD YMENGDLNQF LSARQLENKA 730 740 750 760 770 780 TQGLSGDTES DQGPTISYPM LLHVGAQIAS GMRYLATLNF VHRDLATRNC LVGENFTIKI 790 800 810 820 830 840 ADFGMSRNLY AGDYYRVQGR AVLPIRWMAW ECILMGKFTT ASDVWAFGVT LWEVLMLCRS 850 860 870 880 890 900 QPFGQLTDEQ VIENAGEFFR DQGRQVYLSR PPACPQTLYE LMLRCWSREP EQRPPFAQLH 910 RFLADDALNT V
	Isoform 2 (CAK II). Checksum: A649B2FCE360BA5B	FASTA	874	97,207
	10 20 30 40 50 60 MGTGTLSSLL LLLLLVTIGD ADMKGHFDPA KCRYALGMQD RTIPDSDISV SSSWSDSTAA 70 80 90 100 110 120 RHSRLESSDG DGAWCPAGPV FPKEEEYLQV DLRRLHLVAL VGTQGRHAGG LGKEFSRSYR 130 140 150 160 170 180 LRYSRDGRRW MDWKDRWGQE VISGNEDPGG VVLKDLGPPM VARLVRFYPR ADRVMSVCLR 190 200 210 220 230 240 VELYGCLWRD GLLSYTAPVG QTMQLSEVMV HLNDSTYDGY TAGGLQYGGL GQLADGVVGL 250 260 270 280 290 300 DDFRQSQELR VWPGYDYVGW SNQSFPTGYV EMEFEFDRLR TFQTMQVHCN NMHTLGARLP 310 320 330 340 350 360 GGVECRFKRG PAMAWEGEPV RHALGGSLGD PRARAISVPL GGHVGRFLQC RFLFAGPWLL 370 380 390 400 410 420 FSEISFISDV VNDSSDTFPP APWWPPGPPP TNFSSLELEP RGQQPVAKAE GSPTAILIGC 430 440 450 460 470 480 LVAIILLLLL IIALMLWRLH WRRLLSKAER RVLEEELTVH LSVPGDTILI NNRPGPREPP 490 500 510 520 530 540 PYQEPRPRGT PPHSAPCVPN GSACSGDYME PEKPGAPLLP PPPQNSVPHY AEADIVTLQG 550 560 570 580 590 600 VTGGNTYAVP ALPPGAVGDG PPRVDFPRSR LRFKEKLGEG QFGEVHLCEV EDPQDLVSSD 610 620 630 640 650 660 FPISVHKGHP LLVAVKILRP DATKNARNDF LKEVKIMSRL KDPNIIRLLG VCVQDDPLCM 670 680 690 700 710 720 ITDYMENGDL NQFLSARQLE NKATQGLSGD TESDQGPTIS YPMLLHVGAQ IASGMRYLAT 730 740 750 760 770 780 LNFVHRDLAT RNCLVGENFT IKIADFGMSR NLYAGDYYRV QGRAVLPIRW MAWECILMGK 790 800 810 820 830 840 FTTASDVWAF GVTLWEVLML CRSQPFGQLT DEQVIENAGE FFRDQGRQVY LSRPPACPQT 850 860 870 LYELMLRCWS REPEQRPPFA QLHRFLADDA LNTV

References

[1]	"Identification of two isoforms of the Cak receptor kinase that are coexpressed in breast tumor cell lines." Perez J.L., Jing S.Q., Wong T.W. Oncogene 12:1469-1477(1996) [PubMed: 8622863] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6.
[2]	"An Eph-related receptor protein tyrosine kinase gene segmentally expressed in the developing mouse hindbrain." Gilardi-Hebenstreit P., Nieto M.A., Frain M., Mattei M.-G., Chestier A., Wilkinson D.G., Charnay P. Oncogene 7:2499-2506(1992) [PubMed: 1281307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 766-822. Strain: C57BL/6. Tissue: Embryonic brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L57509 mRNA. Translation: AAB05209.1. X57240 mRNA. Translation: CAA40516.1.
IPI	IPI00129221. IPI00226685.
PIR	S30502.
RefSeq	NP_031610.1. NP_766550.1.
UniGene	Mm.5021
3D structure databases
SMR	Q03146. Positions 28-187, 45-275.
ModBase	Search...
Protein-protein interaction databases
STRING	Q03146.
PTM databases
PhosphoSite	Q03146.
Proteomic databases
PRIDE	Q03146.
Genome annotation databases
Ensembl	ENSMUST00000003628; ENSMUSP00000003628; ENSMUSG00000003534; Mus musculus. [Genome view] ENSMUST00000097333; ENSMUSP00000094945; ENSMUSG00000003534; Mus musculus. [Genome view] ENSMUST00000117301; ENSMUSP00000112570; ENSMUSG00000003534; Mus musculus. [Genome view] ENSMUST00000119825; ENSMUSP00000113062; ENSMUSG00000003534; Mus musculus. [Genome view]
GeneID	12305.
KEGG	mmu:12305.
UCSC	uc008cih.1. mouse.
Organism-specific databases
CTD	12305.
MGI	MGI:99216. Ddr1.
Phylogenomic databases
HOGENOM	HBG387802.
HOVERGEN	Q03146.
InParanoid	Q03146.
OMA	LNVRKGH.
OrthoDB	EOG9X9B2Z.
PhylomeDB	Q03146.
Enzyme and pathway databases
BRENDA	2.7.10.1. 244.
Gene expression databases
ArrayExpress	Q03146.
Bgee	Q03146.
CleanEx	MM_DDR1.
Genevestigator	Q03146.
GermOnline	ENSMUSG00000003534. Mus musculus.
Family and domain databases
InterPro	IPR000421. Coagulation_factor_5/8-type_C. IPR008979. Galactose-bd-like. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. IPR002011. Tyr_prot_kinase_rcpt_2_CS. [Graphical view]
PANTHER	PTHR23256. Tyr_prot_kinase. 1 hit.
Pfam	PF00754. F5_F8_type_C. 1 hit. [Graphical view]
SMART	SM00231. FA58C. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS01285. FA58C_1. 1 hit. PS01286. FA58C_2. 1 hit. PS50022. FA58C_3. 1 hit. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS00239. RECEPTOR_TYR_KIN_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	280834.
SOURCE	Search...

Entry information

Entry name

DDR1_MOUSE

Accession

Primary (citable) accession number: Q03146

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	November 1, 1997
Last modified:	February 9, 2010
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)