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Q03141

- MARK3_MOUSE

UniProt

Q03141 - MARK3_MOUSE

Protein

MAP/microtubule affinity-regulating kinase 3

Gene

Mark3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    Involved in the specific phosphorylation of microtubule-associated proteins for tau, MAP2 and MAP4. Phosphorylates CDC25C. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by phosphorylation on Thr-211. Inhibited by phosphorylation on Thr-564 By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei85 – 851ATPPROSITE-ProRule annotation
    Active sitei178 – 1781Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi62 – 709ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein serine/threonine kinase activity Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    MAP/microtubule affinity-regulating kinase 3 (EC:2.7.11.1)
    Alternative name(s):
    ELKL motif kinase 2
    Short name:
    EMK-2
    MPK-10
    Gene namesi
    Name:Mark3
    Synonyms:Emk2, Mpk10
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:1341865. Mark3.

    Subcellular locationi

    Cell membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 753753MAP/microtubule affinity-regulating kinase 3PRO_0000086305Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei42 – 421PhosphoserineBy similarity
    Modified residuei211 – 2111Phosphothreonine; by LKB1By similarity
    Modified residuei383 – 3831PhosphoserineBy similarity
    Modified residuei400 – 4001PhosphoserineBy similarity
    Modified residuei469 – 4691PhosphoserineBy similarity
    Modified residuei549 – 5491PhosphothreonineBy similarity
    Modified residuei564 – 5641Phosphothreonine; by PKC/PRKCZBy similarity
    Modified residuei598 – 5981PhosphoserineBy similarity
    Modified residuei643 – 6431PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-564 by PRKCZ/aPKC inhibits the kinase activity By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ03141.
    PRIDEiQ03141.

    PTM databases

    PhosphoSiteiQ03141.

    Expressioni

    Gene expression databases

    ArrayExpressiQ03141.
    BgeeiQ03141.
    CleanExiMM_MARK3.
    GenevestigatoriQ03141.

    Interactioni

    Protein-protein interaction databases

    BioGridi201312. 6 interactions.
    IntActiQ03141. 1 interaction.

    Structurei

    Secondary structure

    1
    753
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi672 – 68514
    Beta strandi689 – 6924
    Beta strandi698 – 7025
    Beta strandi704 – 7063
    Helixi707 – 7093
    Beta strandi711 – 7199
    Beta strandi721 – 73515
    Helixi737 – 75014

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1UL7NMR-A659-753[»]
    1V5SNMR-A674-753[»]
    ProteinModelPortaliQ03141.
    SMRiQ03141. Positions 1-366, 657-753.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ03141.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini56 – 307252Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini326 – 36540UBAPROSITE-ProRule annotationAdd
    BLAST
    Domaini695 – 74450KA1PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi374 – 41239Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 KA1 (kinase-associated) domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 UBA domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00730000110535.
    HOGENOMiHOG000233025.
    HOVERGENiHBG052453.
    InParanoidiQ03141.
    KOiK08798.
    OMAiTFHGQLR.
    OrthoDBiEOG79CXXX.
    PhylomeDBiQ03141.
    TreeFamiTF315213.

    Family and domain databases

    Gene3Di3.30.310.80. 1 hit.
    InterProiIPR028375. KA1/Ssp2_C.
    IPR001772. KA1_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    [Graphical view]
    PfamiPF02149. KA1. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    SM00165. UBA. 1 hit.
    [Graphical view]
    SUPFAMiSSF103243. SSF103243. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS50032. KA1. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50030. UBA. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q03141-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSTRTPLPTV NERDTENHIS HGDGRQEVTS RTGRSGARCR NSIASCADEQ    50
    PHIGNYRLLK TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL 100
    FREVRIMKIL NHPNIVKLFE VIETEKTLYL IMEYASGGEV FDYLVAHGRM 150
    KEKEARAKFR QIVSAVQYCH QKRIVHRDLK AENLLLDADM NIKIADFGFS 200
    NEFTVGSKLD TFCGSPPYAA PELFQGKKYD GPEVDVWSLG VILYTLVSGS 250
    LPFDGQNLKE LRERVLRGKY RIPFYMSTDC ENLLKRFLVL NPVKRGTLEQ 300
    IMKDRWINAG HEEDELKPFV EPELDISDQK RIDIMVGMGY SQEEIQESLS 350
    KMKYDEITAT YLLLGRKSAE LDASDSSSSS NLSLAKVRPN SDLSNSTGQS 400
    PHHKGQRSVS SSQKQRRYSD HAGPAIPSVV AYPKRSQTST ADSDLKEDGI 450
    PSRKSSSSAV GGKGIAPASP MLGNAGNPNK ADIPERKKSP AVPSSNTASG 500
    GMTRRNTYVC SERCAADRHS VIQNGKENSA IPDERTPVAS THSISSATTP 550
    DRIRFPRGTA SRSTFHGQPR ERRTATYNGP PASPSLSHEA TPLSQTRSRG 600
    STNLFSKLTS KLTRRNMSFR FIKRLPTEYE RNGRYEGSSR NVSSEQKDEN 650
    REAKPRSLRF TWSMKTTSSM DPSDMMREIR KVLDANNCDY EQRERFLLFC 700
    VHGDGHAENL VQWEMEVCKL PRLSLNGVRF KRISGTSIAF KNIASKIANE 750
    LKL 753
    Length:753
    Mass (Da):84,390
    Last modified:November 8, 2005 - v2
    Checksum:iB597BDD0398617CF
    GO
    Isoform 2 (identifier: Q03141-2) [UniParc]FASTAAdd to Basket

    Also known as: ELKL motif kinase 2 short form

    The sequence of this isoform differs from the canonical sequence as follows:
         615-638: Missing.

    Show »
    Length:729
    Mass (Da):81,400
    Checksum:iD76FBB9A27ED19C9
    GO
    Isoform 3 (identifier: Q03141-3) [UniParc]FASTAAdd to Basket

    Also known as: ELKL motif kinase 2 long form

    The sequence of this isoform differs from the canonical sequence as follows:
         615-623: Missing.

    Show »
    Length:744
    Mass (Da):83,209
    Checksum:i2CCA8FA8AEE20360
    GO

    Sequence cautioni

    The sequence AAH26445.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAC35922.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti333 – 3331D → V in BAC35922. (PubMed:16141072)Curated
    Sequence conflicti496 – 4961N → S in AAF64456. 1 PublicationCurated
    Sequence conflicti511 – 5111S → G in BAC35922. (PubMed:16141072)Curated
    Sequence conflicti684 – 6841D → G in BAC35922. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei615 – 63824Missing in isoform 2. 3 PublicationsVSP_016140Add
    BLAST
    Alternative sequencei615 – 6239Missing in isoform 3. 2 PublicationsVSP_016141

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF240783 mRNA. Translation: AAF64456.1.
    AK075742 mRNA. Translation: BAC35922.1. Different initiation.
    AK132247 mRNA. Translation: BAE21056.1.
    AK166157 mRNA. Translation: BAE38602.1.
    BC026445 mRNA. Translation: AAH26445.1. Different initiation.
    X57244 mRNA. Translation: CAA40520.1.
    CCDSiCCDS26181.1. [Q03141-3]
    CCDS26182.1. [Q03141-2]
    PIRiS30496.
    RefSeqiNP_067491.2. NM_021516.4. [Q03141-3]
    NP_073712.2. NM_022801.4. [Q03141-2]
    XP_006515578.1. XM_006515515.1. [Q03141-1]
    UniGeneiMm.260504.
    Mm.420865.

    Genome annotation databases

    EnsembliENSMUST00000075281; ENSMUSP00000074757; ENSMUSG00000007411. [Q03141-2]
    ENSMUST00000084953; ENSMUSP00000082017; ENSMUSG00000007411. [Q03141-3]
    GeneIDi17169.
    KEGGimmu:17169.
    UCSCiuc007pdk.2. mouse. [Q03141-3]
    uc007pdl.2. mouse. [Q03141-2]
    uc007pdm.2. mouse. [Q03141-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF240783 mRNA. Translation: AAF64456.1 .
    AK075742 mRNA. Translation: BAC35922.1 . Different initiation.
    AK132247 mRNA. Translation: BAE21056.1 .
    AK166157 mRNA. Translation: BAE38602.1 .
    BC026445 mRNA. Translation: AAH26445.1 . Different initiation.
    X57244 mRNA. Translation: CAA40520.1 .
    CCDSi CCDS26181.1. [Q03141-3 ]
    CCDS26182.1. [Q03141-2 ]
    PIRi S30496.
    RefSeqi NP_067491.2. NM_021516.4. [Q03141-3 ]
    NP_073712.2. NM_022801.4. [Q03141-2 ]
    XP_006515578.1. XM_006515515.1. [Q03141-1 ]
    UniGenei Mm.260504.
    Mm.420865.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1UL7 NMR - A 659-753 [» ]
    1V5S NMR - A 674-753 [» ]
    ProteinModelPortali Q03141.
    SMRi Q03141. Positions 1-366, 657-753.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201312. 6 interactions.
    IntActi Q03141. 1 interaction.

    PTM databases

    PhosphoSitei Q03141.

    Proteomic databases

    PaxDbi Q03141.
    PRIDEi Q03141.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000075281 ; ENSMUSP00000074757 ; ENSMUSG00000007411 . [Q03141-2 ]
    ENSMUST00000084953 ; ENSMUSP00000082017 ; ENSMUSG00000007411 . [Q03141-3 ]
    GeneIDi 17169.
    KEGGi mmu:17169.
    UCSCi uc007pdk.2. mouse. [Q03141-3 ]
    uc007pdl.2. mouse. [Q03141-2 ]
    uc007pdm.2. mouse. [Q03141-1 ]

    Organism-specific databases

    CTDi 4140.
    MGIi MGI:1341865. Mark3.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00730000110535.
    HOGENOMi HOG000233025.
    HOVERGENi HBG052453.
    InParanoidi Q03141.
    KOi K08798.
    OMAi TFHGQLR.
    OrthoDBi EOG79CXXX.
    PhylomeDBi Q03141.
    TreeFami TF315213.

    Miscellaneous databases

    ChiTaRSi MARK3. mouse.
    EvolutionaryTracei Q03141.
    NextBioi 291458.
    PROi Q03141.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q03141.
    Bgeei Q03141.
    CleanExi MM_MARK3.
    Genevestigatori Q03141.

    Family and domain databases

    Gene3Di 3.30.310.80. 1 hit.
    InterProi IPR028375. KA1/Ssp2_C.
    IPR001772. KA1_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    [Graphical view ]
    Pfami PF02149. KA1. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    SM00165. UBA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103243. SSF103243. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS50032. KA1. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50030. UBA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse EMK2 (ELKL motif kinase 2)."
      Darmon Y., Tornier C., Bessone S., Le Morvan V.
      Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
      Strain: C57BL/6J.
      Tissue: Embryo, Lung and Placenta.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 168-753 (ISOFORM 2).
      Strain: FVB/N.
      Tissue: Mammary gland.
    4. "An Eph-related receptor protein tyrosine kinase gene segmentally expressed in the developing mouse hindbrain."
      Gilardi-Hebenstreit P., Nieto M.A., Frain M., Mattei M.-G., Chestier A., Wilkinson D.G., Charnay P.
      Oncogene 7:2499-2506(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 180-234.
      Strain: C57BL/6.
      Tissue: Embryonic brain.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "Solution structure of the kinase-associated domain 1 of mouse microtubule-associated protein/microtubule affinity-regulating kinase 3."
      Tochio N., Koshiba S., Kobayashi N., Inoue M., Yabuki T., Aoki M., Seki E., Matsuda T., Tomo Y., Motoda Y., Kobayashi A., Tanaka A., Hayashizaki Y., Terada T., Shirouzu M., Kigawa T., Yokoyama S.
      Protein Sci. 15:2534-2543(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 659-753.

    Entry informationi

    Entry nameiMARK3_MOUSE
    AccessioniPrimary (citable) accession number: Q03141
    Secondary accession number(s): Q3TM40
    , Q3V1U3, Q8C6G9, Q8R375, Q9JKE4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3