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Q03141 (MARK3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
MAP/microtubule affinity-regulating kinase 3

EC=2.7.11.1
Alternative name(s):
ELKL motif kinase 2
Short name=EMK-2
MPK-10
Gene names
Name:Mark3
Synonyms:Emk2, Mpk10
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length753 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the specific phosphorylation of microtubule-associated proteins for tau, MAP2 and MAP4. Phosphorylates CDC25C. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by phosphorylation on Thr-211. Inhibited by phosphorylation on Thr-564 By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein By similarity.

Post-translational modification

Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-564 by PRKCZ/aPKC inhibits the kinase activity By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Contains 1 KA1 (kinase-associated) domain.

Contains 1 protein kinase domain.

Contains 1 UBA domain.

Sequence caution

The sequence AAH26445.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC35922.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q03141-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q03141-2)

Also known as: ELKL motif kinase 2 short form;

The sequence of this isoform differs from the canonical sequence as follows:
     615-638: Missing.
Isoform 3 (identifier: Q03141-3)

Also known as: ELKL motif kinase 2 long form;

The sequence of this isoform differs from the canonical sequence as follows:
     615-623: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 753753MAP/microtubule affinity-regulating kinase 3
PRO_0000086305

Regions

Domain56 – 307252Protein kinase
Domain326 – 36540UBA
Domain695 – 74450KA1
Nucleotide binding62 – 709ATP By similarity
Compositional bias374 – 41239Ser-rich

Sites

Active site1781Proton acceptor By similarity
Binding site851ATP By similarity

Amino acid modifications

Modified residue421Phosphoserine By similarity
Modified residue2111Phosphothreonine; by LKB1 By similarity
Modified residue3831Phosphoserine By similarity
Modified residue4001Phosphoserine By similarity
Modified residue4691Phosphoserine By similarity
Modified residue5491Phosphothreonine By similarity
Modified residue5641Phosphothreonine; by PKC/PRKCZ By similarity
Modified residue5981Phosphoserine By similarity
Modified residue6431Phosphoserine By similarity

Natural variations

Alternative sequence615 – 63824Missing in isoform 2.
VSP_016140
Alternative sequence615 – 6239Missing in isoform 3.
VSP_016141

Experimental info

Sequence conflict3331D → V in BAC35922. Ref.2
Sequence conflict4961N → S in AAF64456. Ref.1
Sequence conflict5111S → G in BAC35922. Ref.2
Sequence conflict6841D → G in BAC35922. Ref.2

Secondary structure

................ 753
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 8, 2005. Version 2.
Checksum: B597BDD0398617CF

FASTA75384,390
        10         20         30         40         50         60 
MSTRTPLPTV NERDTENHIS HGDGRQEVTS RTGRSGARCR NSIASCADEQ PHIGNYRLLK 

        70         80         90        100        110        120 
TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL FREVRIMKIL NHPNIVKLFE 

       130        140        150        160        170        180 
VIETEKTLYL IMEYASGGEV FDYLVAHGRM KEKEARAKFR QIVSAVQYCH QKRIVHRDLK 

       190        200        210        220        230        240 
AENLLLDADM NIKIADFGFS NEFTVGSKLD TFCGSPPYAA PELFQGKKYD GPEVDVWSLG 

       250        260        270        280        290        300 
VILYTLVSGS LPFDGQNLKE LRERVLRGKY RIPFYMSTDC ENLLKRFLVL NPVKRGTLEQ 

       310        320        330        340        350        360 
IMKDRWINAG HEEDELKPFV EPELDISDQK RIDIMVGMGY SQEEIQESLS KMKYDEITAT 

       370        380        390        400        410        420 
YLLLGRKSAE LDASDSSSSS NLSLAKVRPN SDLSNSTGQS PHHKGQRSVS SSQKQRRYSD 

       430        440        450        460        470        480 
HAGPAIPSVV AYPKRSQTST ADSDLKEDGI PSRKSSSSAV GGKGIAPASP MLGNAGNPNK 

       490        500        510        520        530        540 
ADIPERKKSP AVPSSNTASG GMTRRNTYVC SERCAADRHS VIQNGKENSA IPDERTPVAS 

       550        560        570        580        590        600 
THSISSATTP DRIRFPRGTA SRSTFHGQPR ERRTATYNGP PASPSLSHEA TPLSQTRSRG 

       610        620        630        640        650        660 
STNLFSKLTS KLTRRNMSFR FIKRLPTEYE RNGRYEGSSR NVSSEQKDEN REAKPRSLRF 

       670        680        690        700        710        720 
TWSMKTTSSM DPSDMMREIR KVLDANNCDY EQRERFLLFC VHGDGHAENL VQWEMEVCKL 

       730        740        750 
PRLSLNGVRF KRISGTSIAF KNIASKIANE LKL 

« Hide

Isoform 2 (ELKL motif kinase 2 short form) [UniParc].

Checksum: D76FBB9A27ED19C9
Show »

FASTA72981,400
Isoform 3 (ELKL motif kinase 2 long form) [UniParc].

Checksum: 2CCA8FA8AEE20360
Show »

FASTA74483,209

References

« Hide 'large scale' references
[1]"Mouse EMK2 (ELKL motif kinase 2)."
Darmon Y., Tornier C., Bessone S., Le Morvan V.
Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Strain: C57BL/6J.
Tissue: Embryo, Lung and Placenta.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 168-753 (ISOFORM 2).
Strain: FVB/N.
Tissue: Mammary gland.
[4]"An Eph-related receptor protein tyrosine kinase gene segmentally expressed in the developing mouse hindbrain."
Gilardi-Hebenstreit P., Nieto M.A., Frain M., Mattei M.-G., Chestier A., Wilkinson D.G., Charnay P.
Oncogene 7:2499-2506(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 180-234.
Strain: C57BL/6.
Tissue: Embryonic brain.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"Solution structure of the kinase-associated domain 1 of mouse microtubule-associated protein/microtubule affinity-regulating kinase 3."
Tochio N., Koshiba S., Kobayashi N., Inoue M., Yabuki T., Aoki M., Seki E., Matsuda T., Tomo Y., Motoda Y., Kobayashi A., Tanaka A., Hayashizaki Y., Terada T., Shirouzu M., Kigawa T., Yokoyama S.
Protein Sci. 15:2534-2543(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 659-753.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF240783 mRNA. Translation: AAF64456.1.
AK075742 mRNA. Translation: BAC35922.1. Different initiation.
AK132247 mRNA. Translation: BAE21056.1.
AK166157 mRNA. Translation: BAE38602.1.
BC026445 mRNA. Translation: AAH26445.1. Different initiation.
X57244 mRNA. Translation: CAA40520.1.
PIRS30496.
RefSeqNP_067491.2. NM_021516.4.
NP_073712.2. NM_022801.4.
XP_006515578.1. XM_006515515.1.
UniGeneMm.260504.
Mm.420865.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1UL7NMR-A659-753[»]
1V5SNMR-A674-753[»]
ProteinModelPortalQ03141.
SMRQ03141. Positions 1-366, 657-753.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201312. 6 interactions.
IntActQ03141. 1 interaction.

PTM databases

PhosphoSiteQ03141.

Proteomic databases

PaxDbQ03141.
PRIDEQ03141.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000075281; ENSMUSP00000074757; ENSMUSG00000007411. [Q03141-2]
ENSMUST00000084953; ENSMUSP00000082017; ENSMUSG00000007411. [Q03141-3]
GeneID17169.
KEGGmmu:17169.
UCSCuc007pdk.2. mouse. [Q03141-3]
uc007pdl.2. mouse. [Q03141-2]
uc007pdm.2. mouse. [Q03141-1]

Organism-specific databases

CTD4140.
MGIMGI:1341865. Mark3.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00730000110535.
HOGENOMHOG000233025.
HOVERGENHBG052453.
InParanoidQ03141.
KOK08798.
OMAEDGISSR.
OrthoDBEOG79CXXX.
PhylomeDBQ03141.
TreeFamTF315213.

Gene expression databases

ArrayExpressQ03141.
BgeeQ03141.
CleanExMM_MARK3.
GenevestigatorQ03141.

Family and domain databases

Gene3D3.30.310.80. 1 hit.
InterProIPR028375. KA1/Ssp2_C.
IPR001772. KA1_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR015940. UBA/transl_elong_EF1B_N_euk.
[Graphical view]
PfamPF02149. KA1. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMSSF103243. SSF103243. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS50032. KA1. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMARK3. mouse.
EvolutionaryTraceQ03141.
NextBio291458.
PROQ03141.
SOURCESearch...

Entry information

Entry nameMARK3_MOUSE
AccessionPrimary (citable) accession number: Q03141
Secondary accession number(s): Q3TM40 expand/collapse secondary AC list , Q3V1U3, Q8C6G9, Q8R375, Q9JKE4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 8, 2005
Last modified: April 16, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot