Reviewed, UniProtKB/Swiss-Prot Q03134 (FDH_EMENI)

Names and origin

Protein names

Recommended name:
    Probable formate dehydrogenase
    EC=1.2.1.2
Alternative name(s):
    NAD-dependent formate dehydrogenase
      Short name=FDH

Gene names

Name:	aciA
ORF Names:	AN6525

Organism

Emericella nidulans (Aspergillus nidulans)

Taxonomic identifier

162425 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Formate + NAD+ = CO2 + NADH.
Subunit structure	Homodimer By similarity.
Induction	Acetate induction mediated by amda regulatory gene.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FDH subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro formate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Probable formate dehydrogenase		PRO_0000076024
Sites
	Active site	271	1	By similarity
	Active site	324	1	Proton donor By similarity
Experimental info
	Sequence conflict	330 – 331	2	ID → LA in CAA77687. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q03134-1 [UniParc]. Last modified May 26, 2009. Version 2. Checksum: 3972984EBCC935DE	FASTA	377	41,575
	10 20 30 40 50 60 MVLYDGGSHA KDQPGLLGTT ENELGIRKWI EEQGHTLVTT SDKDGENSTF DKELVDAEVI 70 80 90 100 110 120 ITTPFHPGYL TAERLAKAKN LKLAVTAGIG SDHVDLDAAN KTNGGITVAE VTGSNVVSVA 130 140 150 160 170 180 EHVVMTILLL VRNFVPAHDQ IRNGDWNVAA VAKNEFDLEN KVVGTVGVGR IGERVLRRLK 190 200 210 220 230 240 PFDCKELLYY DYQPLRPEVE KEIGARRVDS LEEMVSQCDV VTINCPLHEK TRGLFNKELI 250 260 270 280 290 300 SKMKPGKSAL LYLIIPMLMY HKGSWLVNTA RGAIVVKEDV AEALKSGHLR GYGGDVWFPQ 310 320 330 340 350 360 PAPKEHPLRY AEHPWGGGNA TVPHMSGTSI DAQIRYANGT KAILDSYFSG RFDYQPQDLI 370 VHGGDYATKA YGQREKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the amdA-regulated aciA gene of Aspergillus nidulans." Saleeba J.A., Cobbett C.S., Hynes M.J. Mol. Gen. Genet. 235:349-358(1992) [PubMed: 1465107] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC 4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z11612 Genomic DNA. Translation: CAA77687.1. AACD01000109 Genomic DNA. Translation: EAA57865.1.
PIR	S30088.
3D structure databases
HSSP	HSSP built from PDB template 2NAC based on UniProtKB P33160.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.2.1.2. 3859.
Family and domain databases
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDH_EMENI

Accession

Primary (citable) accession number: Q03134
Secondary accession number(s): Q5AYV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	May 26, 2009
Last modified:	June 16, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)