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Protein

Formate dehydrogenase

Gene

aciA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the NAD+-dependent oxidation of formate to carbon dioxide. Formate oxidation is the final step in the methanol oxidation pathway in methylotrophic microorganisms. Has a role in the detoxification of exogenous formate in non-methylotrophic organisms.UniRule annotation

Catalytic activityi

Formate + NAD+ = CO2 + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei94 – 941Substrate; via amide nitrogenUniRule annotation
Binding sitei120 – 1201SubstrateUniRule annotation
Binding sitei196 – 1961NADUniRule annotation
Binding sitei257 – 2571NAD; via carbonyl oxygenUniRule annotation
Sitei259 – 2591Important for catalytic activityUniRule annotation
Binding sitei283 – 2831NADUniRule annotation
Sitei312 – 3121Important for catalytic activityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi175 – 1762NADUniRule annotation
Nucleotide bindingi231 – 2355NADUniRule annotation
Nucleotide bindingi312 – 3154NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

  • acetate metabolic process Source: ASPGD
  • formate catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Formate dehydrogenaseUniRule annotation (EC:1.2.1.2UniRule annotation)
Short name:
FDHUniRule annotation
Alternative name(s):
Acetate inducible protein A1 Publication
NAD-dependent formate dehydrogenaseUniRule annotation
Gene namesi
Name:aciA1 Publication
ORF Names:AN6525
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome I
  • UP000005890 Componenti: Partially assembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:AN6525.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: GO_Central
  • extracellular region Source: ASPGD
  • intracellular Source: ASPGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365Formate dehydrogenasePRO_0000076024Add
BLAST

Proteomic databases

PRIDEiQ03134.

Expressioni

Inductioni

Acetate induction mediated by the amdA regulatory gene.1 Publication

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ03134.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni3 – 120118CatalyticUniRule annotationAdd
BLAST
Regioni121 – 313193Coenzyme-bindingUniRule annotationAdd
BLAST
Regioni314 – 35946CatalyticUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FDH subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000136703.
InParanoidiQ03134.
KOiK00122.
OMAiELIYYDY.
OrthoDBiEOG092C30ZL.

Family and domain databases

CDDicd05302. FDH. 1 hit.
Gene3Di3.40.50.720. 2 hits.
HAMAPiMF_03210. Formate_dehydrogenase. 1 hit.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR029752. D-isomer_DH_CS1.
IPR006140. D-isomer_DH_NAD-bd.
IPR033689. FDH_NAD-dep.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q03134-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKVLMVLYD GGSHAKDQPG LLGTTENELG IRKWIEEQGH TLVTTSDKDG
60 70 80 90 100
ENSTFDKELV DAEVIITTPF HPGYLTAERL AKAKNLKLAV TAGIGSDHVD
110 120 130 140 150
LDAANKTNGG ITVAEVTGSN VVSVAEHVVM TILLLVRNFV PAHDQIRNGD
160 170 180 190 200
WNVAAVAKNE FDLENKVVGT VGVGRIGERV LRRLKPFDCK ELLYYDYQPL
210 220 230 240 250
RPEVEKEIGA RRVDSLEEMV SQCDVVTINC PLHEKTRGLF NKELISKMKP
260 270 280 290 300
GSWLVNTARG AIVVKEDVAE ALKSGHLRGY GGDVWFPQPA PKEHPLRYAE
310 320 330 340 350
HPWGGGNATV PHMSGTSIDA QIRYANGTKA ILDSYFSGRF DYQPQDLIVH
360
GGDYATKAYG QREKK
Length:365
Mass (Da):40,131
Last modified:November 16, 2011 - v3
Checksum:iC9AB2B4EFBFC540D
GO

Sequence cautioni

The sequence CAA77687 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence EAA57865 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11612 Genomic DNA. Translation: CAA77687.1. Sequence problems.
AACD01000109 Genomic DNA. Translation: EAA57865.1. Sequence problems.
BN001301 Genomic DNA. Translation: CBF70916.1.
PIRiS30088.
RefSeqiXP_664129.1. XM_659037.1.

Genome annotation databases

EnsemblFungiiCADANIAT00007297; CADANIAP00007297; CADANIAG00007297.
EAA57865; EAA57865; AN6525.2.
GeneIDi2870218.
KEGGiani:AN6525.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11612 Genomic DNA. Translation: CAA77687.1. Sequence problems.
AACD01000109 Genomic DNA. Translation: EAA57865.1. Sequence problems.
BN001301 Genomic DNA. Translation: CBF70916.1.
PIRiS30088.
RefSeqiXP_664129.1. XM_659037.1.

3D structure databases

ProteinModelPortaliQ03134.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ03134.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00007297; CADANIAP00007297; CADANIAG00007297.
EAA57865; EAA57865; AN6525.2.
GeneIDi2870218.
KEGGiani:AN6525.2.

Organism-specific databases

EuPathDBiFungiDB:AN6525.

Phylogenomic databases

HOGENOMiHOG000136703.
InParanoidiQ03134.
KOiK00122.
OMAiELIYYDY.
OrthoDBiEOG092C30ZL.

Family and domain databases

CDDicd05302. FDH. 1 hit.
Gene3Di3.40.50.720. 2 hits.
HAMAPiMF_03210. Formate_dehydrogenase. 1 hit.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR029752. D-isomer_DH_CS1.
IPR006140. D-isomer_DH_NAD-bd.
IPR033689. FDH_NAD-dep.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFDH_EMENI
AccessioniPrimary (citable) accession number: Q03134
Secondary accession number(s): C8V0K7, Q5AYV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 16, 2011
Last modified: September 7, 2016
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.