Reviewed, UniProtKB/Swiss-Prot Q03124 (RSC9_YEAST)

Names and origin

Protein names

Recommended name:
    Chromatin structure-remodeling complex subunit RSC9
Alternative name(s):
    RSC complex subunit RSC9
    Remodel the structure of chromatin complex subunit 9

Gene names

Name:	RSC9
Ordered Locus Names:	YML127W
ORF Names:	YM4987.08

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	581 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit plays a role in transcriptional response to stress. It is involved in both repression and activation of mRNAs regulated by the target of rapamycin (TOR) kinases, and in the synthesis of rRNA. Ref.2 Ref.3 Ref.4 Ref.5 Ref.7 Ref.8 Ref.9
Subunit structure	Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin.
Subcellular location	Nucleus. Note: Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1. Ref.2 Ref.9
Miscellaneous	Present with 2610 molecules/cell in log phase SD medium. Ref.10
Sequence similarities	Belongs to the RSC9 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation rRNA processing
Cellular component	Nucleus
Molecular function	Chromatin regulator
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ATP-dependent chromatin remodeling Ref.4 Ref.7 Inferred from direct assay. Source: UniProtKB G2/M transition of mitotic cell cycle Inferred from mutant phenotype. Source: SGD RNA elongation from RNA polymerase II promoter Inferred from direct assay. Source: SGD nucleosome disassembly Inferred from direct assay. Source: SGD rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW rRNA transcription Ref.2 Inferred from mutant phenotype. Source: UniProtKB regulation of transcription from RNA polymerase II promoter Ref.2 Inferred from mutant phenotype. Source: SGD response to stress Ref.2 Inferred from direct assay. Source: UniProtKB
Cellular component	RSC complex Ref.2 Ref.6 Ref.7 Inferred from direct assay. Source: UniProtKB
Molecular function	chromatin binding Ref.2 Inferred from physical interaction. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RSC8	P43609	1	EBI-27977,EBI-23005

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 581	581	Chromatin structure-remodeling complex subunit RSC9		PRO_0000097475
Amino acid modifications
	Modified residue	22	1	Phosphoserine Ref.12
	Modified residue	44	1	Phosphoserine Ref.12
	Modified residue	513	1	Phosphoserine Ref.11
Experimental info
	Sequence conflict	60	1	Y → T AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q03124-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 04EA7901486656AB	FASTA	581	65,218
	10 20 30 40 50 60 MNSLASNTPL NGTPVSEAPA TSSEPVNMFE TMVANPIKVS RLQSNGVLTG PAANTKSIHY 70 80 90 100 110 120 SLANFNVFQS LPKETARGVD DLTRMEMALL SGIPEEIKWS LKKYLTYSNK APYMISLRTL 130 140 150 160 170 180 PDLLPLFKTF ILPLERIVEG LNKSSICDSK AMDSLQMGLN ALLILRNLAQ DTDSVQILVK 190 200 210 220 230 240 DREIKSFILF ILKKFQCVAT GDNKWQLYEG NATFFNELTH YTLDLMEAIS SYIAPAMKDD 250 260 270 280 290 300 HYFQTLVSIL NYTKDRYMVI SILRSLSRLL VRSKANEESA ADNLDHKTLS LIVSFLLLEC 310 320 330 340 350 360 DSELIIASLD FLYQYILPGS QRITELFKSK ECSLILEATL PNLLSYNIAT PDYHLLQKHK 370 380 390 400 410 420 IRLIKRLKPP APKEPPNLSE DLFQQLFKLN EPLRSTAWLR CCFEPVQEAE FTQISLWRSY 430 440 450 460 470 480 ESKFGQPVRE SGRKLLPAVE FIKNVSNAFN NAAAIVITDP VTGKKRFVIK GIQPRFKALG 490 500 510 520 530 540 IADGERESQV PISALKSKFL NDSKEITPAR QNSIPEVKFP QELSDVSKVA CTFLCLLSND 550 560 570 580 TDDGAGSAFC QRIRPLVLHK LADIPPLTLA LSEYMENTSG L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The genome-wide localization of Rsc9, a component of the RSC chromatin-remodeling complex, changes in response to stress." Damelin M., Simon I., Moy T.I., Wilson B., Komili S., Tempst P., Roth F.P., Young R.A., Cairns B.R., Silver P.A. Mol. Cell 9:563-573(2002) [PubMed: 11931764] [Abstract] Cited for: PROTEIN SEQUENCE OF 57-73 AND 401-418, FUNCTION, IDENTIFICATION IN THE RSC COMPLEX, SUBCELLULAR LOCATION.
[3]	"RSC, an essential, abundant chromatin-remodeling complex." Cairns B.R., Lorch Y., Li Y., Zhang M., Lacomis L., Erdjument-Bromage H., Tempst P., Du J., Laurent B.C., Kornberg R.D. Cell 87:1249-1260(1996) [PubMed: 8980231] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX, COMPOSITION OF THE RSC COMPLEX.
[4]	"Histone octamer transfer by a chromatin-remodeling complex." Lorch Y., Zhang M., Kornberg R.D. Cell 96:389-392(1999) [PubMed: 10025404] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[5]	"Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC." Moreira J.M.A., Holmberg S. EMBO J. 18:2836-2844(1999) [PubMed: 10329629] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[6]	"Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains." Cairns B.R., Schlichter A., Erdjument-Bromage H., Tempst P., Kornberg R.D., Winston F. Mol. Cell 4:715-723(1999) [PubMed: 10619019] [Abstract] Cited for: COMPOSITION OF THE RSC COMPLEX.
[7]	"Chromatin remodeling by RSC involves ATP-dependent DNA translocation." Saha A., Wittmeyer J., Cairns B.R. Genes Dev. 16:2120-2134(2002) [PubMed: 12183366] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[8]	"Yeast RSC function is required for organization of the cellular cytoskeleton via an alternative PKC1 pathway." Chai B., Hsu J.-M., Du J., Laurent B.C. Genetics 161:575-584(2002) [PubMed: 12072455] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[9]	"The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation." Hsu J.-M., Huang J., Meluh P.B., Laurent B.C. Mol. Cell. Biol. 23:3202-3215(2003) [PubMed: 12697820] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX, SUBCELLULAR LOCATION, INTERACTION OF THE RSC COMPLEX WITH HISTONES.
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-513, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22 AND SER-44, MASS SPECTROMETRY.
[13]	"Structural analysis of the yeast SWI/SNF chromatin remodeling complex." Smith C.L., Horowitz-Scherer R., Flanagan J.F., Woodcock C.L., Peterson C.L. Nat. Struct. Biol. 10:141-145(2003) [PubMed: 12524530] [Abstract] Cited for: 3D-STRUCTURE MODELING OF THE SWI/SNF COMPLEX, ELECTRON MICROSCOPY OF THE SWI/SNF COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z50178 Genomic DNA. Translation: CAA90556.1.
PIR	S58201.
RefSeq	NP_013579.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4517N.
IntAct	Q03124. 13 interactions.
STRING	Q03124.
Proteomic databases
PeptideAtlas	Q03124.
PRIDE	Q03124.
Genome annotation databases
Ensembl	YML127W; YML127W; YML127W; Saccharomyces cerevisiae. [Genome view]
GeneID	854912.
KEGG	sce:YML127W.
NMPDR	fig|4932.3.peg.4615.
Organism-specific databases
CYGD	YML127w.
SGD	S000004596. RSC9.
Phylogenomic databases
HOGENOM	Q03124.
OMA	LYQYILP
OrthoDB	EOG97H765
Gene expression databases
ArrayExpress	Q03124.
Genevestigator	Q03124.
GermOnline	YML127W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	977911.

Entry information

Entry name

RSC9_YEAST

Accession

Primary (citable) accession number: Q03124

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)