Reviewed, UniProtKB/Swiss-Prot Q03113 (GNA12_HUMAN)

Names and origin

Protein names

Recommended name:
    Guanine nucleotide-binding protein subunit alpha-12
      Short name=G-protein subunit alpha-12
      Short name=G alpha-12

Gene names

Name:

GNA12

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
Subunit structure	G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with UBXD5.
Subcellular location	Membrane; Lipid-anchor.
Sequence similarities	Belongs to the G-alpha family. G(12) subfamily.

Ontologies

Keywords
Cellular component	Membrane
Ligand	GTP-binding Nucleotide-binding
Molecular function	Transducer
PTM	Lipoprotein Palmitate
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Traceable author statement. Source: ProtInc blood coagulation Traceable author statement. Source: ProtInc
Cellular component	plasma membrane Inferred from Experiment. Source: Reactome
Molecular function	GTP binding Inferred from electronic annotation. Source: InterPro GTPase activity Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: UniProtKB signal transducer activity Non-traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 381	380	Guanine nucleotide-binding protein subunit alpha-12		PRO_0000203770
Regions
	Nucleotide binding	64 – 71	8	GTP By similarity
	Nucleotide binding	227 – 231	5	GTP By similarity
	Nucleotide binding	296 – 299	4	GTP By similarity
Amino acid modifications
	Lipidation	11	1	S-palmitoyl cysteine By similarity
Experimental info
	Sequence conflict	126	1	K → E in AAM12615. Ref.2
	Sequence conflict	323	1	R → Q in AAA35867. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q03113-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 04C3F12B29255F89	FASTA	381	44,279
	10 20 30 40 50 60 MSGVVRTLSR CLLPAEAGGA RERRAGSGAR DAEREARRRS RDIDALLARE RRAVRRLVKI 70 80 90 100 110 120 LLLGAGESGK STFLKQMRII HGREFDQKAL LEFRDTIFDN ILKGSRVLVD ARDKLGIPWQ 130 140 150 160 170 180 YSENEKHGMF LMAFENKAGL PVEPATFQLY VPALSALWRD SGIREAFSRR SEFQLGESVK 190 200 210 220 230 240 YFLDNLDRIG QLNYFPSKQD ILLARKATKG IVEHDFVIKK IPFKMVDVGG QRSQRQKWFQ 250 260 270 280 290 300 CFDGITSILF MVSSSEYDQV LMEDRRTNRL VESMNIFETI VNNKLFFNVS IILFLNKMDL 310 320 330 340 350 360 LVEKVKTVSI KKHFPDFRGD PHRLEDVQRY LVQCFDRKRR NRSKPLFHHF TTAIDTENVR 370 380 FVFHAVKDTI LQENLKDIML Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression cDNA cloning of a transforming gene encoding the wild-type G alpha 12 gene product." Chan A.M.-L., Fleming T.P., McGovern E.S., Chedid M., Miki T., Aaronson S.A. Mol. Cell. Biol. 13:762-768(1993) [PubMed: 8423800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Socius, a novel binding partner of Galpha12/13, promotes the Galpha12-induced RhoA activation." Tateiwa K., Katoh H., Negishi M. Biochem. Biophys. Res. Commun. 337:615-620(2005) [PubMed: 16202387] [Abstract] Cited for: INTERACTION WITH UBXD5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L01694 mRNA. Translation: AAA35867.1. AF493901 mRNA. Translation: AAM12615.1. AC006028 Genomic DNA. Translation: AAP21870.1. AC004933 Genomic DNA. Translation: AAD05026.1. BC111464 mRNA. Translation: AAI11465.1.
PIR	A48071.
RefSeq	NP_031379.2.
UniGene	Hs.487341
3D structure databases
HSSP	HSSP built from PDB template 1CUL based on UniProtKB P04896.
SMR	Q03113. Positions 56-373.
ModBase	Search...
PTM databases
PhosphoSite	Q03113.
Genome annotation databases
Ensembl	ENSG00000146535. Homo sapiens. [Contig view]
GeneID	2768.
KEGG	hsa:2768.
Organism-specific databases
H-InvDB	HIX0006438.
HGNC	HGNC:4380. GNA12.
HPA	CAB010046.
MIM	604394. gene.
PharmGKB	PA28765.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q03113.
HOVERGEN	Q03113.
Enzyme and pathway databases
Reactome	REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	Q03113.
CleanEx	HS_GNA12.
GermOnline	ENSG00000146535. Homo sapiens.
Family and domain databases
InterPro	IPR001019. Gprotein_alph_bd. IPR000469. Gprotein_alpha12. [Graphical view]
PANTHER	PTHR10218. Gprotein_alph_bd. 1 hit.
Pfam	PF00503. G-alpha. 1 hit. [Graphical view]
PRINTS	PR00318. GPROTEINA. PR00440. GPROTEINA12.
ProDom	PD000281. Gprotein_alpha. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other Resources
NextBio	10888.
SOURCE	Search...

Entry information

Entry name

GNA12_HUMAN

Accession

Primary (citable) accession number: Q03113
Secondary accession number(s): Q2T9L1 , Q86UM8, Q8TD71, Q9UDU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 79 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)